- Email: [email protected]
P1078 Vaccination of healthcare workers against hepatitis B virus in a teaching hospital
S290 512 which shows 30 times increase in titer when 3 I/M injection of 50 mg of conjugate were given to animals. Discussion: This finding reveals that I/M injection of covalently bound purified Vi-CPS to a T-dependent proteins elicited much higher levels of antibodies than Vi-CPS alone besides, this conjugate it induce a booster responses in human. But, unfortunately the level of conjugation in this process is low due to les number of protein binding sites on Vi-molecules. Though the Vi-CPS conjugates elicits higher levels of antibodies, immunoprophylactic activities of the conjugate must be as curtained by clinical evaluation and more research should be conducted to increase the degree of conjugation of the two macromolecules. P1077 Immunogenicity of tetanus component in monovalent and combined vaccines W. Janaszek-Seydlitz, B. Bucholc, U. Czajka, A. Wiatrzyk (Warsaw, PL) Objectives: Vaccination with combined vaccines is without any doubt beneficial, allowing for decreasing number of injections and costs connected with vaccination. However, there is a possibility that the interaction between components of combined vaccines may influence their immunogenicity and reactogenicity. The immunogenicity of tetanus component in monovalent tetanus toxoid adsorbed and combined vaccines DTP and DTP+IPV was evaluated on Balb/c mice. Methods: Animals: Six weeks old Balb/c mice were immunised with tetanus toxoid adsorbed; DTP or DTP+IPV. Animals (10 mice in each group) were injected subcutaneously three times with dose 0.25 ml at 6 weeks intervals. Control was unvaccinated. Enzyme-linked immunosorbent assay (ELISA): The antibody responses to tetanus toxoid in sera 14 days after the immunisation were determined using in house ELISA method. The level of antibodies was expressed in arbitrary units. Production of IFN-g and IL-5: The levels of IFN-g and IL-5 were evaluated in supernatants of cell culture of splenocytes stimulated by non-adsorbed tetanus toxoid or PHA. Results: Geometric mean titre for anti-tetanus antibodies evaluated 14 days after the immunisation in animals vaccinated with tetanus toxoid adsorbed was lower (2650.2 a.u./mL) than in animals vaccinated with DTP (3968.6 a.u./mL) or DTP+IPV (4070.7 a.u./mL). There was no statistical differences between groups of animals receiving only DTP or simultaneously DTP and IPV. The mean concentration of IFN-g in supernatants from specific stimulated cell cultures derived from animals vaccinated with tetanus toxoid was 720.45 pg/mL, from animals vaccinated with DTP – 2579.7 pg/mL and from animals vaccinated with DTP+IPV 1812.05 pg/mL. The mean concentrations of IL-5 are about 20-times lower than concentration of IFN-g in cell cultures of splenocytes derived from all evaluated groups of animals. Conclusions: Whole-cell pertussis component influences as adjuvant on tetanus component in combined vaccines. Simultaneously administrated IPV vaccine not influences on immunogenicity of tetanus component. Pertussis component significantly increases level of specific tetanus antibodies and production of IFN-g in cell cultures derived from vaccinated animals. P1078 Vaccination of healthcare workers against hepatitis B virus in a teaching hospital C. Ataman Hatipoglu, M.A. Yetkin, F. Ergin, K. Ipekkan, F.S. Erdinc, C. Bulut, N. Tulek, G. Demir, Y. Yigit, A.P. Demiroz (Ankara, Samsun, TR) Objectives: Hepatitis B virus infection which can be prevented by active immunisation is one of the common health problems among the healthcare workers (HCW). The aim of this study was to vaccinate our HCWs in order to protect them against hepatitis B virus. Methods: A total of 1009 HCWs who were working in Ankara Teaching and Research Hospital during January 2002-June 2006 were included into the study. HBsAg and antiHBs results, demographic data (gender,
17th ECCMID / 25th ICC, Posters age, occupation and department) and immune status against hepatitis B virus were recorded for each HCW. HCWs that were negative for HBsAg and antiHBs were included into the vaccination programme. Results: Among 1009 HCWs, 448 were male (44.4%), 561 female (55.6%) and the mean age was 32.02±7.74 years. Of the HCWs, 209 were working as doctors (20.7%), 246 as nurses (24.4%), 322 as domestic workers (31.9%) and 232 (23.0%) as other works (laboratory technician, radiology technician, officer, etc). Thirty-one percent of HCWs were working in the surgical departments whereas 20.6% of them in internal medicine departments. The rest of them were working in laboratories, emergency department, outpatient clinics and various other parts of the hospital with a rate of 8.4%, 7.4%, 7.3% and 25.5%, respectively. HBsAg was negative in all HCWs. AntiHBs was positive in 439 (43.5%) and negative in 570 (56.5%). A total of 570 HCWs were included in the vaccination programme. One hundred and fifteen (20.2%) were vaccinated with three-dose regimen in 0, 1 and 6 months, 349 (61.2%) with four-dose regimen in 0, 1, 2 and 12 months and 106 (18.6%) with single booster dose with yeast derived hepatitis B vaccine (Euvax-B, LG Chemical, S. Corea). Four hundred and sixtynine of 1009 HCWs had a history of vaccination before, 170 (36.3%) of them had negative value of antiHBs at the time of the study. These 170 HCWs were vaccinated with a single dose hepatitis B vaccine. One hundred and six of them became positive for anti HBs after this vaccination. A complete vaccination schedule was applied to the remaining 64 HCWs who were negative after a single dose. Three hundred and sixty HCWs (63.1%) were compatible with the vaccination programme whereas 210 (36.9%) of them were incompatible. Conclusion: We think that systematic surveillance and vaccination against hepatitis B will reduce the risk of transmission of this infection to the HCWs. P1079 Comparing the immunogenecity of hepatitis B vaccine in two different methods of intramuscular and intradermal injection in infants of Bandar Abbas city, Iran P. Davoodian, M. Jamshidi, Z. Shamandi, K. Kargar, K. Mahouri (Bandar Abbas, IR) Objectives: The efficacy and safety of recombinant DNA hepatitis B vaccine is well known. However, few data are available on comparasion of different injection methods of interadermal and intra muscular. This study aimed to make a comparasion between immunogenicity of these two methods of injection in infants. Methods: In a clinical trial 47 cases entered the study who were infants randomly selected and received Intra-dermal(ID) immunisation against Hepatitis B at the ages of 0, 1.5 and 9 months old. The blood samples were obtained from our cases at the age of one year. The control group was consisted of 100 blood samples which were obtained from 1 year old infants who had given blood sample for other purposes. These infants were immunised according to the national vaccination routines applying intra-mascular(IM) injection method, 3 times (0, 1.5 and 9 months old), against HBV. After collecting the blood samples from case and control groups all samples were tested with ELISA method to determine antiHBs Ab level. Results: 84% of cases in IM and 87/3% in ID method had protective immunity (p = 0.791). In IM method 41.7% of cases had complete immunity whereas only 19.5% of those with ID method had complete immunity against HBV (p = 0.024). There was no relation between gender and Ab level in any of the injection methods. Conclusion: We found out that both IM and ID methods have the same immunogenecity feature but IM method resulted in more complete immunity in infants.
17th ECCMID / 25th ICC, Posters age, occupation and department) and immune status against hepatitis B virus were recorded for each HCW. HCWs that were negative for HBsAg and antiHBs were included into the vaccination programme. Results: Among 1009 HCWs, 448 were male (44.4%), 561 female (55.6%) and the mean age was 32.02±7.74 years. Of the HCWs, 209 were working as doctors (20.7%), 246 as nurses (24.4%), 322 as domestic workers (31.9%) and 232 (23.0%) as other works (laboratory technician, radiology technician, officer, etc). Thirty-one percent of HCWs were working in the surgical departments whereas 20.6% of them in internal medicine departments. The rest of them were working in laboratories, emergency department, outpatient clinics and various other parts of the hospital with a rate of 8.4%, 7.4%, 7.3% and 25.5%, respectively. HBsAg was negative in all HCWs. AntiHBs was positive in 439 (43.5%) and negative in 570 (56.5%). A total of 570 HCWs were included in the vaccination programme. One hundred and fifteen (20.2%) were vaccinated with three-dose regimen in 0, 1 and 6 months, 349 (61.2%) with four-dose regimen in 0, 1, 2 and 12 months and 106 (18.6%) with single booster dose with yeast derived hepatitis B vaccine (Euvax-B, LG Chemical, S. Corea). Four hundred and sixtynine of 1009 HCWs had a history of vaccination before, 170 (36.3%) of them had negative value of antiHBs at the time of the study. These 170 HCWs were vaccinated with a single dose hepatitis B vaccine. One hundred and six of them became positive for anti HBs after this vaccination. A complete vaccination schedule was applied to the remaining 64 HCWs who were negative after a single dose. Three hundred and sixty HCWs (63.1%) were compatible with the vaccination programme whereas 210 (36.9%) of them were incompatible. Conclusion: We think that systematic surveillance and vaccination against hepatitis B will reduce the risk of transmission of this infection to the HCWs. P1079 Comparing the immunogenecity of hepatitis B vaccine in two different methods of intramuscular and intradermal injection in infants of Bandar Abbas city, Iran P. Davoodian, M. Jamshidi, Z. Shamandi, K. Kargar, K. Mahouri (Bandar Abbas, IR) Objectives: The efficacy and safety of recombinant DNA hepatitis B vaccine is well known. However, few data are available on comparasion of different injection methods of interadermal and intra muscular. This study aimed to make a comparasion between immunogenicity of these two methods of injection in infants. Methods: In a clinical trial 47 cases entered the study who were infants randomly selected and received Intra-dermal(ID) immunisation against Hepatitis B at the ages of 0, 1.5 and 9 months old. The blood samples were obtained from our cases at the age of one year. The control group was consisted of 100 blood samples which were obtained from 1 year old infants who had given blood sample for other purposes. These infants were immunised according to the national vaccination routines applying intra-mascular(IM) injection method, 3 times (0, 1.5 and 9 months old), against HBV. After collecting the blood samples from case and control groups all samples were tested with ELISA method to determine antiHBs Ab level. Results: 84% of cases in IM and 87/3% in ID method had protective immunity (p = 0.791). In IM method 41.7% of cases had complete immunity whereas only 19.5% of those with ID method had complete immunity against HBV (p = 0.024). There was no relation between gender and Ab level in any of the injection methods. Conclusion: We found out that both IM and ID methods have the same immunogenecity feature but IM method resulted in more complete immunity in infants.