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P141 Overexpression of cyclin E and centrosome hyperamplification in oral squamous cell carcinomas
174 cell adhesion molecules in the subcellular extractions of cancer cells by immunoblotting. Results: The basal expression level of p53R2 showed no association with the mutational status of p53. The invasive potential of cancer cells also showed no association with the mutational status of p53. However, the cancer cell lines with higher p53R2 expression were more invasive potential (p < 0.05). RNAi-mediated p53R2 reduction decreased the invasion index (p < 0.01), and inhibited nuclear translocation of beta-catenin. Discussion: These results suggested that expression of p53R2 could be associated with the invasion of cancer cell. We indicated that p53R2 might promote cancer invasion via the beta-catenin pathway. Keywords: p53R2, invasion, RNAi, cell adhesion molecule P141 Overexpression of cyclin E and centrosome hyperamplification in oral squamous cell carcinomas C. Yang *, L. Yongfa, Z. Yan, Y. Yanni. Guiyang Medical College, China Objective: It is currently considered that Cdk2 activated by Cyclin E might be the key event for the initial of centrosome duplication. This study is an attempt to elucidate the possible role of Cyclin E in centrosome amplification of oral squamous cell carcinoma (OSCC) in vivo. Methods: Formalin-fixed, paraffin-embedded tissues of 12 cases of normal oral epithelium and 46 cases of OSCC were investigated for centrosome status by using indirect Immunofluorescence double staining with antibodies to centrosome protein gamma-tubulin and keratinizing squamous cell protein cytokeratin, while the expression of Cyclin E protein in the same tissues was investigated by Immunohistochemistry. The correlation between the expression of Cyclin E and centrosome amplification in OSCC were statistically analyzed by SPSS12.0. Results: All normal oral epithelium showed normal size and number of centrosomes in epithelium cells, while 37 out of 46 cases (80.43%) of OSCC showed the evidence of centrosome amplification characterized by huge size and/or supernumerary centrosomes in a fraction of tumor cells. Negative staining of Cyclin E was also seen in all normal oral epithelium, while positive staining of Cyclin E was seen in 30 out of 46 cases (65.63%) of OSCC. The percentage of OSCC with centrosome amplification in the group of positive Cyclin E staining was 90% (27/30), which was higher than that in the group of negative Cyclin E staining 62.5% (10/16) (c2 = 5.014, P < 0.05). Centrosome hyperamplification was associated with Cyclin E overexpression (Spearman r=0.330, p < 0.05) and the Odds Ratio of Risk Estimate was 5.400 (1.130, 25.809) in OSCC investigated. Conclusions: Up-regulated Cyclin E is likely to a contributing factor towards centrosome amplification in tumour cells, which may be one of the potential mechanisms for centrosome amplification in OSCC. Keywords: oral squamous cell carcinoma (OSCC), centrosome, Cyclin E, oral cancer P142 Expression of Ki-67 and endoglin (CD 105) proteins in stages I and II squamous cell carcinoma of oral tongue and floor of the mouth: association with demographic, clinical and histopathological factors prognostic analysis
Poster abstracts, Saturday 19 May Methods: Fifty-three patients were treated at Cancer Hospital of Pernambuco, between May 1996 and February 2002 and were analyzed by using immunohistochemical staining. The markers and the other factors were correlated with tumoral recurrence and overall survival. Results: Perineural (p = 0.0038) and vascular invasion (p = 0.0070), male sex (p = 0.0371) and morphologic aspect exophytic-ulcerative(p = 0.0016) had impact in overall survival. Only perineural invasion (p = 0.009) was statistically significant for recurrence of disease. Endoglin expression in tumor tissue was significantly higher than in normal healthy mucosa (p = 0.0201). Conclusion: Perineural and vascular invasion, male sex and morphologic aspect were consistently prognostic factors in this study. In addition, levels of endoglin expression were higher in tumor specimens, what indicate that endoglin is a powerful marker of neovascularization in solid malignancies. Keywords: Ki-67, Endoglin, Prognosis, Oral cancer P143 Angiogenesis and prognosis in oral cavity cancer a study of 53 patients F. Matos1 *, L. Brand˜ ao2 , F. Dias3 , R. Lima3 , S. Vasconcellos1 , 1 T. Santiago , M. do Carmo Carvalho4 . 1 IMIP/PE, Brazil, 2 USP, Brazil, 3 INCA/RJ, Brazil, 4 UFPE, Brazil Introduction: Angiogenesis is a mechanism which is essential for tumor growth and metastatis. Endoglin (CD105) is a proliferation-associated protein expressed in angiogenic endothelial cells and used as a marker of neovascularization in solid malignancies. The purpose of this retrospective study was to evaluate the prognostic role of CD105 and association with other factors in squamous cell carcinoma of oral tongue and floor of the mouth, stage I and II. Methods: We retrospectively reviewed 53 patients treated at Cancer Hospital of Pernambuco, between May 1996 and February 2002. The records of these cases were reviewed to determine demographic (age, sex and race), clinical (duration of the affection and primary site) and histopathological (morphologic aspect, grade of the tumors, perineural invasion and vascular invasion) factors. Paraffin-embedded sections were investigated quantitatively by immunohistochemistry using monoclonal antibody against CD105. The marker and the other factors were correlated with tumoral recurrence, overall survival and disease free survival. Results: Endoglin expression in tumor tissue was significant higher than in the normal mucosa (p = 0.0201). Perineural (p = 0.0038) and vascular invasion (p = 0.007), male sex (p = 0.0371) and morphologic aspect ulcerative-exophytic (p = 0.0016) had impact in overall survival. Only perineural invasion (p = 0.009) was statistically significant for recurrence of disease. Conclusion: Perineural and vascular invasion, male sex and morphologic aspect were consistently prognostic factors in this study. Endoglin expression is higher in tissues with squamous cell carcinoma compared with healthy oral mucosa. This finding suggests that endoglin is a powerful marker of neovascularization in solid malignancies. Keywords: Angiogenesis, CD 105, Prognosis, Oral Cancer P144 Cellular proliferation in oral cavity cancer, stage I and II prognostic analysis
F. Matos1 *, L. Brand˜ ao2 , F. Dias3 , R. Lima3 , S. Vasconcellos1 , T. Santiago1 , M. do Carmo Carvalho4 . 1 IMIP/PE, Brazil, 2 USP, Brazil, 3 INCA/RJ, Brazil, 4 UFPE, Brazil
F. Matos1 *, L. Brand˜ ao2 , F. Dias3 , R. Lima3 , S. Vasconcellos1 , T. Santiago1 , M. do Carmo Carvalho4 . 1 IMIP/PE, Brazil, 2 USP, Brazil, 3 INCA/RJ, Brazil, 4 UFPE, Brazil
Objective: This study retrospectively examines the prognostic role of Ki-67 and endoglin (CD 105) and association with other factors in squamous cell carcinoma of oral tongue and floor of the mouth.
Introduction: Ki-67 is used as a marker of cellular proliferation, expressed in all phases of the cellular cicle except in GO. The purpose of this retrospective study was to evaluate the prognostic role of Ki-67 and association with other factors in
Poster abstracts, Saturday 19 May Methods: Fifty-three patients were treated at Cancer Hospital of Pernambuco, between May 1996 and February 2002 and were analyzed by using immunohistochemical staining. The markers and the other factors were correlated with tumoral recurrence and overall survival. Results: Perineural (p = 0.0038) and vascular invasion (p = 0.0070), male sex (p = 0.0371) and morphologic aspect exophytic-ulcerative(p = 0.0016) had impact in overall survival. Only perineural invasion (p = 0.009) was statistically significant for recurrence of disease. Endoglin expression in tumor tissue was significantly higher than in normal healthy mucosa (p = 0.0201). Conclusion: Perineural and vascular invasion, male sex and morphologic aspect were consistently prognostic factors in this study. In addition, levels of endoglin expression were higher in tumor specimens, what indicate that endoglin is a powerful marker of neovascularization in solid malignancies. Keywords: Ki-67, Endoglin, Prognosis, Oral cancer P143 Angiogenesis and prognosis in oral cavity cancer a study of 53 patients F. Matos1 *, L. Brand˜ ao2 , F. Dias3 , R. Lima3 , S. Vasconcellos1 , 1 T. Santiago , M. do Carmo Carvalho4 . 1 IMIP/PE, Brazil, 2 USP, Brazil, 3 INCA/RJ, Brazil, 4 UFPE, Brazil Introduction: Angiogenesis is a mechanism which is essential for tumor growth and metastatis. Endoglin (CD105) is a proliferation-associated protein expressed in angiogenic endothelial cells and used as a marker of neovascularization in solid malignancies. The purpose of this retrospective study was to evaluate the prognostic role of CD105 and association with other factors in squamous cell carcinoma of oral tongue and floor of the mouth, stage I and II. Methods: We retrospectively reviewed 53 patients treated at Cancer Hospital of Pernambuco, between May 1996 and February 2002. The records of these cases were reviewed to determine demographic (age, sex and race), clinical (duration of the affection and primary site) and histopathological (morphologic aspect, grade of the tumors, perineural invasion and vascular invasion) factors. Paraffin-embedded sections were investigated quantitatively by immunohistochemistry using monoclonal antibody against CD105. The marker and the other factors were correlated with tumoral recurrence, overall survival and disease free survival. Results: Endoglin expression in tumor tissue was significant higher than in the normal mucosa (p = 0.0201). Perineural (p = 0.0038) and vascular invasion (p = 0.007), male sex (p = 0.0371) and morphologic aspect ulcerative-exophytic (p = 0.0016) had impact in overall survival. Only perineural invasion (p = 0.009) was statistically significant for recurrence of disease. Conclusion: Perineural and vascular invasion, male sex and morphologic aspect were consistently prognostic factors in this study. Endoglin expression is higher in tissues with squamous cell carcinoma compared with healthy oral mucosa. This finding suggests that endoglin is a powerful marker of neovascularization in solid malignancies. Keywords: Angiogenesis, CD 105, Prognosis, Oral Cancer P144 Cellular proliferation in oral cavity cancer, stage I and II prognostic analysis
F. Matos1 *, L. Brand˜ ao2 , F. Dias3 , R. Lima3 , S. Vasconcellos1 , T. Santiago1 , M. do Carmo Carvalho4 . 1 IMIP/PE, Brazil, 2 USP, Brazil, 3 INCA/RJ, Brazil, 4 UFPE, Brazil
F. Matos1 *, L. Brand˜ ao2 , F. Dias3 , R. Lima3 , S. Vasconcellos1 , T. Santiago1 , M. do Carmo Carvalho4 . 1 IMIP/PE, Brazil, 2 USP, Brazil, 3 INCA/RJ, Brazil, 4 UFPE, Brazil
Objective: This study retrospectively examines the prognostic role of Ki-67 and endoglin (CD 105) and association with other factors in squamous cell carcinoma of oral tongue and floor of the mouth.
Introduction: Ki-67 is used as a marker of cellular proliferation, expressed in all phases of the cellular cicle except in GO. The purpose of this retrospective study was to evaluate the prognostic role of Ki-67 and association with other factors in