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P.167 The adequate choice of bone graft for reconstruction in maxillofacial region
Posters P.166 Salivary urea, creatinine, ascorbic and oxalic acid in CRF patients A. Jenca1 , J. Jencova1 , M. Mydlik2 , K. Derziova1 . 1 Department of stomatology and maxillofacial surgery, 2 Department of ˇ arik University in Koˇsice, Slovakia nephrology, Pavol Jozef Saf´ Oxalic acid is well-known uremic toxin. Aim of the study was the investigation of salivary secretion of oxalic (OA) and ascorbic acid (AA) in 37 patients (pts) with various glomerular filtration rate (GFR). Among them were 13 pts with normal GFR who presented a control group (CG) and 24 pts suffered from chronic renal failure (CRF) of various origin. Whole saliva was collected without and with exogeneous stimulation. In both groups of pts plasma (Pl) and salivary (Sl) OA were determined, by spectrophotometric method using oxalate oxidase, and Pl and Sl AA, by spectrophotometric method. Direct relationships (R) between Pl AA and Sl AA (r = 0.71, p < 0.01), between Pl urea and Sl urea (r = 0.78, p < 0.01), and between Sl pH and Sl urea (r = 0.53, p < 0.01) in CRF pts were found. Indirect R between Pl OA and Sl OA (r=−0.51, p < 0.05), between Sl pH and Sl OA (r=−0.41, p < 0.05), and between Sl creatinine and Sl OA (r=−0.40, p < 0.05) were found in the same group of pts. Conclusion: (1) Salivary urea and creatinine were increased in CRF pts. (2) Despite high level of Pl OA in CRF pts, the concentration of OA in saliva was in the normal range. (3) The Sl AA concentration was very low and it was in direct R with Pl AA in CRF pts. (4) The value of Sl pH in CRF pts was significantly alkaline.
P.167 The adequate choice of bone graft for reconstruction in maxillofacial region M. Orencak1 , A. Rosocha2 , J. Jencova1 , Y. Ismail1 . 1 Department of stomatology and maxillofacial surgery, 2 Tissue bank of ˇ arik University in Koˇsice, Medical faculty, Pavol Jozef Saf´ Slovakia Aim: Various bone-graft techniques have been utilized by oral and maxillofacial surgeons for the reconstruction of the maxilla and mandible over the past decades. Insufficient attention has been given to the development of bone-grafting techniques that would have the end result of increasing the bone density of the restored area. Material and Methods: Loss of bone in orofacial region as a result of bone pathology or trauma require surgical therapy using various types of materials and tissues. Each defect poses a unique set of problems that reconstructive surgical intervantion must adress. In the period 1998–2004, 240 patients were treated at our clinic. In 128 cases autografts were used and in 112 cases the allografts were used. In 75% of cases supporting of osteointegration with autogenic osteoblasts was used. Result: Advantage of using combination with autogenic osteoblasts was more than 90% and the period of healing activated and time decreases to 27%. Bone grafts serve a combined mechanical and biologic function, depending on the desired clinical outcome, one function may be more important than the other. A complex relationship exists at the host–graft interface, and the desired clinical result, is ensured. Conclusion: Bone grafting is a surgical procedure by which new bone or replacement material are placed into spaces between or around broken bone (fractures) or holes in bone (defects) to aid in healing. The types of bone grafts available for the maxilla and mandible are the autogenous, allogeneic, alloplastic, xenogeneic and isograft.
New technologies 175 P.168 Oral mucous tissue engineering: Design, characterization and application into athymic mice I. Pe˜na, L.M. Junquera, A. Meana, J.C. De Vicente. Diparminpto Cirug´ıa Oral y Maxilofacial, Hospital Universitario Central de Asturias, Oviedo, Spain Introduction: Oral and Maxillofacial surgeons are in need of supply oral mucous tissue losses. We have developed a technique that allows us to get an equivalent within 30 days. We present histological and immunohistochemical characteristics of this equivalent before and 7 days, 15 days, 21 days after grafting it into athymic mice. Material and Method: Previous informed assent, a 5×5 mm oral mucous biopsy and a 27 ml blood sample are obtained from same patient. Fibroblasts and keratinocytes are isolated from biopsy and cultured separately. After both culture’s confluence, corium is developed using plasma from patient’s blood sample. Once the equivalent is developed, sowing both cellular lines over fibrin gel, it is grafted into immunodeficient mice. The histological and immunohistochemical characterization of the equivalent takes place before graft, 7 days, 14 days and 21 days after. A histological (with haematoxylin-eosin), and immunohistochemical study with markers p63 (stem cell marker), Ki-67 (cell proliferation marker), keratin 5 (basal cell marker in polystratified epithelia), keratin 13 (suprabasal cell and cell differentiation marker), AE1/AE3 (keratin type I and II marker), collagen IV and laminin (both basal membrane markers), is done. Results: We have developed an equivalent with histological similarities to oral mucous basal layer. It maintains the proliferation capacity to regenerate the epithelium even if there are both differentiated and non-differentiated cells. There are basal and suprabasal layers at day 15. The keratinocytes can produce a basal membrane and they maintain their anchorage to it through days.
P.169 Ultrasonography of tongue and oral floor – Traditional and using up-to-date modifications I. Rozylo-Kalinowska1 , T.K. Rozylo2 , M. Jurkiewicz-Mazurek2 . 1 2nd Department of Medical Radiology, Medical University of Lublin, Poland; 2 Department of Dental and Maxillofacial Radiology, Medical University of Lublin, Poland Ultrasonographic evaluation of pathologies of tongue and oral floor is difficult due to relatively low prevalence of severe lesions of these strucures, limited conact of general radiologists with such patologies commonly believed to be the domain of dentists as well as poor availability of appropriate machines such as special intraoral ultrasound probes. Traditionally tongue and oral floor tissues are examined ultrasonographically using extraoral approach via oral base. The aim of the study was to determine possibilities of imaging of tongue and oral floor by means of traditional ultrasonography as well as using modifications of the method such as application of stand-off gel pad and three-dimensional imaging. Ultrasonography was performed using linear probes in 15 patients with neoplastic or inflammatory pathologies of tongue and/or oral floor by means of Sonoline G-50 (Siemens) and EUB410 (Hitachi) machines. Furthermore, in 10 healthy young adults three-dimensional ultrasonographic images of the tongue were obtained using a special probe for Voluson 730 Pro (General Electric) ultrasonographic machine and stand-off gel pad. It was proved that ultrasonographic probes designed for volumetric acquisition allow for reconstruction of ultrasonographic images in many planes or their presentation as three-dimensional images, which is advantageous in imaging of the tongue and oral floor. It
P.167 The adequate choice of bone graft for reconstruction in maxillofacial region M. Orencak1 , A. Rosocha2 , J. Jencova1 , Y. Ismail1 . 1 Department of stomatology and maxillofacial surgery, 2 Tissue bank of ˇ arik University in Koˇsice, Medical faculty, Pavol Jozef Saf´ Slovakia Aim: Various bone-graft techniques have been utilized by oral and maxillofacial surgeons for the reconstruction of the maxilla and mandible over the past decades. Insufficient attention has been given to the development of bone-grafting techniques that would have the end result of increasing the bone density of the restored area. Material and Methods: Loss of bone in orofacial region as a result of bone pathology or trauma require surgical therapy using various types of materials and tissues. Each defect poses a unique set of problems that reconstructive surgical intervantion must adress. In the period 1998–2004, 240 patients were treated at our clinic. In 128 cases autografts were used and in 112 cases the allografts were used. In 75% of cases supporting of osteointegration with autogenic osteoblasts was used. Result: Advantage of using combination with autogenic osteoblasts was more than 90% and the period of healing activated and time decreases to 27%. Bone grafts serve a combined mechanical and biologic function, depending on the desired clinical outcome, one function may be more important than the other. A complex relationship exists at the host–graft interface, and the desired clinical result, is ensured. Conclusion: Bone grafting is a surgical procedure by which new bone or replacement material are placed into spaces between or around broken bone (fractures) or holes in bone (defects) to aid in healing. The types of bone grafts available for the maxilla and mandible are the autogenous, allogeneic, alloplastic, xenogeneic and isograft.
New technologies 175 P.168 Oral mucous tissue engineering: Design, characterization and application into athymic mice I. Pe˜na, L.M. Junquera, A. Meana, J.C. De Vicente. Diparminpto Cirug´ıa Oral y Maxilofacial, Hospital Universitario Central de Asturias, Oviedo, Spain Introduction: Oral and Maxillofacial surgeons are in need of supply oral mucous tissue losses. We have developed a technique that allows us to get an equivalent within 30 days. We present histological and immunohistochemical characteristics of this equivalent before and 7 days, 15 days, 21 days after grafting it into athymic mice. Material and Method: Previous informed assent, a 5×5 mm oral mucous biopsy and a 27 ml blood sample are obtained from same patient. Fibroblasts and keratinocytes are isolated from biopsy and cultured separately. After both culture’s confluence, corium is developed using plasma from patient’s blood sample. Once the equivalent is developed, sowing both cellular lines over fibrin gel, it is grafted into immunodeficient mice. The histological and immunohistochemical characterization of the equivalent takes place before graft, 7 days, 14 days and 21 days after. A histological (with haematoxylin-eosin), and immunohistochemical study with markers p63 (stem cell marker), Ki-67 (cell proliferation marker), keratin 5 (basal cell marker in polystratified epithelia), keratin 13 (suprabasal cell and cell differentiation marker), AE1/AE3 (keratin type I and II marker), collagen IV and laminin (both basal membrane markers), is done. Results: We have developed an equivalent with histological similarities to oral mucous basal layer. It maintains the proliferation capacity to regenerate the epithelium even if there are both differentiated and non-differentiated cells. There are basal and suprabasal layers at day 15. The keratinocytes can produce a basal membrane and they maintain their anchorage to it through days.
P.169 Ultrasonography of tongue and oral floor – Traditional and using up-to-date modifications I. Rozylo-Kalinowska1 , T.K. Rozylo2 , M. Jurkiewicz-Mazurek2 . 1 2nd Department of Medical Radiology, Medical University of Lublin, Poland; 2 Department of Dental and Maxillofacial Radiology, Medical University of Lublin, Poland Ultrasonographic evaluation of pathologies of tongue and oral floor is difficult due to relatively low prevalence of severe lesions of these strucures, limited conact of general radiologists with such patologies commonly believed to be the domain of dentists as well as poor availability of appropriate machines such as special intraoral ultrasound probes. Traditionally tongue and oral floor tissues are examined ultrasonographically using extraoral approach via oral base. The aim of the study was to determine possibilities of imaging of tongue and oral floor by means of traditional ultrasonography as well as using modifications of the method such as application of stand-off gel pad and three-dimensional imaging. Ultrasonography was performed using linear probes in 15 patients with neoplastic or inflammatory pathologies of tongue and/or oral floor by means of Sonoline G-50 (Siemens) and EUB410 (Hitachi) machines. Furthermore, in 10 healthy young adults three-dimensional ultrasonographic images of the tongue were obtained using a special probe for Voluson 730 Pro (General Electric) ultrasonographic machine and stand-off gel pad. It was proved that ultrasonographic probes designed for volumetric acquisition allow for reconstruction of ultrasonographic images in many planes or their presentation as three-dimensional images, which is advantageous in imaging of the tongue and oral floor. It