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P.3 Molecular markers as prognostic indicators for local recurrence in oral squamous cell carcinoma
146
Poster Sessions
IP.21 CD40 increases oral keratinocyte migration in vitro M. Vfllarroel-Dorrego, P.M. Spelght, A.W. Barrett. :Oral
and Maxillofaeial Pathology Unit, Eastman Dental Institute, Untversrty College London, Umvers~ty of London, UK. :Dental School, Umvers~dad Central de Venezuela. Caracas. Venezuela
and multivariate analysis showed that these three parameters were independent prognostic factors but that neither p53 nor MIB1 expression was of prognostic value. Conclusion: Patients with T 1 / T 2 0 S C C whose turnouts show high Cyclm D1 and low p21 expressmn at the ITF or those w~th close or positive surgical margins are at higher risk of local recurrence and therefore should be followed carefully to allow early trealrnent of any relapse of their disease.
Introduction: CD40 is a member of the tumour necrosis
factor receptor superfiamily. It is expressed principally by "'professional" antigen presenting cells and plays an important costmlulatory role m T cell activation by binding its llgand CD40L. Approxtmately 80% of oral squamous cell carcinomas (OSCC) are CD40+, as are most oral keratmocyte cell lines. The fimction of CD40 is not only concerned with the immune response; hgatlon of CD40 also regulates cell prollferatlor~ chfferentlatlon and possibly cell rmgratlon. Whilst there are data on the mfluence of some adhesion molecules (malady mtegrms) and thetr llgaslds on the nngratlon of OSCC keratmocytes, the slgmficaslce of CD40 m flus respect is unknown. The am1 of this study was to evalnate the role of CD40 m the migration of oral keratmocytes m vitro. Materials and Methods: A CD40-negatlve keratmocyte line (OSC-19), derived from a metastatic OSCC and kindly donated by Dr. Shuachl Kawashm (University of Kanazawa, Japan), was transfected using the pcDNA3-CD40 plasmad (kindly donated by Dr. Arls E11opoulos University of Btrmmgharn, UK and Dr. Dmmy Huylebroeck Umversaty of Leuven, Belgium). Cell migration was measured on parental, null transfectant control (OSC-19/control) and transfectant (OSC-19/CD40+) cell lanes using TramwellTM membranes coated with fibronectm or Matragel matrax for 4 hours. Results: OSC-19/CD40+ rmgratlon to fibronectm was statastlcally sagmficantly higher than that of OSC-19 (p 0.038) or OSC-19/control (p 0.017) cells. Migration towards the Matragel matrix was statistically hagher 111 OSC-19/CD40+ when these cells were compared to OSC-19 (p=0.004) and OSC-19/control cells (p = 0.013). Conclusion: Enhanced migration of O8C-19/CD40+ suggests that expressaon of CD40 may be anvolved m motlhty of oral keratmocytes
•1
Molecular markers as prognostic indicators for local recurrence in oral squamous cell carcinoma F.A.M. Sawalr, C.R. Irwan, S.S. Napier. :Faculty of Dentzst~y. Untver~ty of Jordan, Amman. Jordan. 2School of Dennstrv. Queen ~ Unwer~ty, Belfast. Ireland Introduction: Expression of p53, Cyclm D1 and p21 (WAF1)
and the prohferatlon marker K>67 (MIB1) was evaluated m oral squamous cell carcinoma (OSCC) to test whether the expressaon of these markers at the mvaslve turnout front (ITF) could predict the development of local recurrence. Materials and Methods: Archlved paraffin-embedded specm~ens from 51 patients with TI/T2 tmnours were stained mamm~ohlstochermcally and analysed quantitatively. Local recurrence-free survwal was tested by Kaplan-Meler survwal plots (log-ras~ test) using the medias1 values to define low and hagh expression groups and with Cox's proportional hazards model in which the expression scores were entered as continuous variables. Results: The assessment of expresmon of all markers was t~ghly rehable, while umvarmte analysis showed that pahents with clear surgical margins, with low Cyclm D1 and high p21 expression at the ITF had the best local recurrence-free survival
~Copy number gains on 22q13 in adenoid cystic carcinoma (ACC) of the salivary gland C. Hofel, K. Freler, S. Joos, C. Flechtenmacher, A. Watch, B. Burke, E Devens, S. Oh1, P. Llchter. :Khntkfiir Mund-,
Ktefer- zmd Geslchtschtrurgte, Umvers~taet Hetdelberg, Germany. :Abtetlung Molekulare Genettk, Deutsches Krebsforschzmgszentrum, Germany. SPathologtsches Instttut, Unwersttaet Hetdelberg, Germcmy, 4patholog,sches Instttut, Unwersttaet Fretburg, Germw~y Introduction: Adenoid cystic carcinoma (ACC) of the salivary
gland as characterazed by slow but inevitable local progression mad late-onset terminal hematogenous metastasas. Materials and Methods: In order to detect novel unbalanced chromosomal regions we screened 27 ACC w~th chromosomal comparative genormc hybrlchzatlon (CGH). Results: The most common aberrahon was copy number gain of 22q13 (9 cases) followed by gains of 16p (7 cases). To fitrther delineate the prevalence of 22ql 3 copy number gains m ACC fluorescence m-sltu hybradlzataon (FISH) was performed for 5 bacterial/phage artificial chromosome (BAC/PAC) probes from the 22q13 regmn with 57 ACC on a hssue rmcroarray (TMA). The results of the FISH asmlysls showed an overall prevalence of copy number gains on 22q13 m 30% of the tumors. C o n d n s | o n : We therefore assume that m some cases copy number gain of 22q13 is mvoNed m tmhal pathogenems of ACC.
I
•
H
P
V
infection in individuals with and without oral squamous cell carcinoma in Mexico: preliminary findings.
G. Anaya-Saavedra, V. Ran~ez-Amador, E. IngoyenCamacho, C. Garcia-Cuellar, M. Gmdo-Junenez, O.K. Luna, A. Mosqueda-Taylor, A. Garcla-Carranca. :Umversldad
Autonoma ?~Ietropohtana-Xochtmllco, ~,Iexlco. etnstttuto Naczonal de Cancerologta, _Mexlco Introduction: High-risk paplllomavaruses (HPV) are assoc>
ated with oral squamous cell carcinoma (OSCC), but the reported prevalence values have been shown great variation, depending on the populatxma, detectmn assays and sample collection tectmaques. In Mexico, there is a lack of 11Yormatlon related with the prevalence of HPV m the oral mucosa. Te aam of the present study is to evaluate the association between HPV types w~th OSCC and other risk factors (sexual behavior, tobacco and alcohol). Materials and Methods: Ongoing case-control study. Parhc1pant subjects were OSCC patmnts who attended the Inshtuto Nacional de Cancerologia in Mexico City, for the first visit. Control subjects, paired by age and sex were selected from a General Hospital in the same geographical area. Age, gender, employment, use of tobacco, alcohol and sexual behavior (number of partners, oral and anal sexual practmes and condom use) were registered. The samples were scraped from the buccal mucosa with a cytobrush and DNA was extracted. The presence
Poster Sessions
IP.21 CD40 increases oral keratinocyte migration in vitro M. Vfllarroel-Dorrego, P.M. Spelght, A.W. Barrett. :Oral
and Maxillofaeial Pathology Unit, Eastman Dental Institute, Untversrty College London, Umvers~ty of London, UK. :Dental School, Umvers~dad Central de Venezuela. Caracas. Venezuela
and multivariate analysis showed that these three parameters were independent prognostic factors but that neither p53 nor MIB1 expression was of prognostic value. Conclusion: Patients with T 1 / T 2 0 S C C whose turnouts show high Cyclm D1 and low p21 expressmn at the ITF or those w~th close or positive surgical margins are at higher risk of local recurrence and therefore should be followed carefully to allow early trealrnent of any relapse of their disease.
Introduction: CD40 is a member of the tumour necrosis
factor receptor superfiamily. It is expressed principally by "'professional" antigen presenting cells and plays an important costmlulatory role m T cell activation by binding its llgand CD40L. Approxtmately 80% of oral squamous cell carcinomas (OSCC) are CD40+, as are most oral keratmocyte cell lines. The fimction of CD40 is not only concerned with the immune response; hgatlon of CD40 also regulates cell prollferatlor~ chfferentlatlon and possibly cell rmgratlon. Whilst there are data on the mfluence of some adhesion molecules (malady mtegrms) and thetr llgaslds on the nngratlon of OSCC keratmocytes, the slgmficaslce of CD40 m flus respect is unknown. The am1 of this study was to evalnate the role of CD40 m the migration of oral keratmocytes m vitro. Materials and Methods: A CD40-negatlve keratmocyte line (OSC-19), derived from a metastatic OSCC and kindly donated by Dr. Shuachl Kawashm (University of Kanazawa, Japan), was transfected using the pcDNA3-CD40 plasmad (kindly donated by Dr. Arls E11opoulos University of Btrmmgharn, UK and Dr. Dmmy Huylebroeck Umversaty of Leuven, Belgium). Cell migration was measured on parental, null transfectant control (OSC-19/control) and transfectant (OSC-19/CD40+) cell lanes using TramwellTM membranes coated with fibronectm or Matragel matrax for 4 hours. Results: OSC-19/CD40+ rmgratlon to fibronectm was statastlcally sagmficantly higher than that of OSC-19 (p 0.038) or OSC-19/control (p 0.017) cells. Migration towards the Matragel matrix was statistically hagher 111 OSC-19/CD40+ when these cells were compared to OSC-19 (p=0.004) and OSC-19/control cells (p = 0.013). Conclusion: Enhanced migration of O8C-19/CD40+ suggests that expressaon of CD40 may be anvolved m motlhty of oral keratmocytes
•1
Molecular markers as prognostic indicators for local recurrence in oral squamous cell carcinoma F.A.M. Sawalr, C.R. Irwan, S.S. Napier. :Faculty of Dentzst~y. Untver~ty of Jordan, Amman. Jordan. 2School of Dennstrv. Queen ~ Unwer~ty, Belfast. Ireland Introduction: Expression of p53, Cyclm D1 and p21 (WAF1)
and the prohferatlon marker K>67 (MIB1) was evaluated m oral squamous cell carcinoma (OSCC) to test whether the expressaon of these markers at the mvaslve turnout front (ITF) could predict the development of local recurrence. Materials and Methods: Archlved paraffin-embedded specm~ens from 51 patients with TI/T2 tmnours were stained mamm~ohlstochermcally and analysed quantitatively. Local recurrence-free survwal was tested by Kaplan-Meler survwal plots (log-ras~ test) using the medias1 values to define low and hagh expression groups and with Cox's proportional hazards model in which the expression scores were entered as continuous variables. Results: The assessment of expresmon of all markers was t~ghly rehable, while umvarmte analysis showed that pahents with clear surgical margins, with low Cyclm D1 and high p21 expression at the ITF had the best local recurrence-free survival
~Copy number gains on 22q13 in adenoid cystic carcinoma (ACC) of the salivary gland C. Hofel, K. Freler, S. Joos, C. Flechtenmacher, A. Watch, B. Burke, E Devens, S. Oh1, P. Llchter. :Khntkfiir Mund-,
Ktefer- zmd Geslchtschtrurgte, Umvers~taet Hetdelberg, Germany. :Abtetlung Molekulare Genettk, Deutsches Krebsforschzmgszentrum, Germany. SPathologtsches Instttut, Unwersttaet Hetdelberg, Germcmy, 4patholog,sches Instttut, Unwersttaet Fretburg, Germw~y Introduction: Adenoid cystic carcinoma (ACC) of the salivary
gland as characterazed by slow but inevitable local progression mad late-onset terminal hematogenous metastasas. Materials and Methods: In order to detect novel unbalanced chromosomal regions we screened 27 ACC w~th chromosomal comparative genormc hybrlchzatlon (CGH). Results: The most common aberrahon was copy number gain of 22q13 (9 cases) followed by gains of 16p (7 cases). To fitrther delineate the prevalence of 22ql 3 copy number gains m ACC fluorescence m-sltu hybradlzataon (FISH) was performed for 5 bacterial/phage artificial chromosome (BAC/PAC) probes from the 22q13 regmn with 57 ACC on a hssue rmcroarray (TMA). The results of the FISH asmlysls showed an overall prevalence of copy number gains on 22q13 m 30% of the tumors. C o n d n s | o n : We therefore assume that m some cases copy number gain of 22q13 is mvoNed m tmhal pathogenems of ACC.
I
•
H
P
V
infection in individuals with and without oral squamous cell carcinoma in Mexico: preliminary findings.
G. Anaya-Saavedra, V. Ran~ez-Amador, E. IngoyenCamacho, C. Garcia-Cuellar, M. Gmdo-Junenez, O.K. Luna, A. Mosqueda-Taylor, A. Garcla-Carranca. :Umversldad
Autonoma ?~Ietropohtana-Xochtmllco, ~,Iexlco. etnstttuto Naczonal de Cancerologta, _Mexlco Introduction: High-risk paplllomavaruses (HPV) are assoc>
ated with oral squamous cell carcinoma (OSCC), but the reported prevalence values have been shown great variation, depending on the populatxma, detectmn assays and sample collection tectmaques. In Mexico, there is a lack of 11Yormatlon related with the prevalence of HPV m the oral mucosa. Te aam of the present study is to evaluate the association between HPV types w~th OSCC and other risk factors (sexual behavior, tobacco and alcohol). Materials and Methods: Ongoing case-control study. Parhc1pant subjects were OSCC patmnts who attended the Inshtuto Nacional de Cancerologia in Mexico City, for the first visit. Control subjects, paired by age and sex were selected from a General Hospital in the same geographical area. Age, gender, employment, use of tobacco, alcohol and sexual behavior (number of partners, oral and anal sexual practmes and condom use) were registered. The samples were scraped from the buccal mucosa with a cytobrush and DNA was extracted. The presence