- Email: [email protected]
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Poster P4:: Wednesday Posters than others. Moreover, 60% cells exhibited typical neuronal morphology, stained positively for markers of GABAergic neurons, GABA⫹ and GAD67⫹ after induction. Conclusions: The human immortalized neural progenitor cells hSN12W-TERT is a potentially powerful in vitro model of GABAergic neuron differentiation. P4-360
THETA OSCILLATIONS ARE PHASE LOCKED AFTER CHOLINESTERASE INHIBITORS IN FRONTAL AREA IN ALZHEIMER’S DISEASE
¨ niz2, Erol Bas¸ar2, Gorsev G. Yener1, Bahar A. Gu¨ntekin2, Adile T. O 1 Dokuz Eylul University; Departments of Neurology and Neural Sciences; Izmir, Multidisciplinary Brain Dynamics Research Center, Turkey; 2Dokuz Eylul University, Multidisciplinary Brain Dynamics ¨ BI˙TAK Brain Dynamics Research Unit, Research Center, Izmir, TU Ankara, Turkey. Contact e-mail: [email protected] Background: Theta oscillations are involved in mnemonic processes. Alzheimer’s disease (AD) is a common amnestic disorder. The current cholinesterase inhibitors may improve cognitive processes and affect the brain oscillations. Objective(s): To compare phase locking of theta event
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related oscillations in non-treated AD and treated AD and healthy elderly controls in frontal areas. Methods: Phase locking of theta (4-7Hz) event related oscillations of 22 mild probable AD patients (11 non-treated and 11 treated with cholinesterase inhibitors) and 20 healthy elderly controls were compared by using conventional visual oddball paradigm. Conclusions: At F3 location, theta responses of healthy subjects show stronger phase locking to stimulation than non-treated AD patients and the treated AD group has theta phase locking as strong as the control subjects. The F4 theta responses were not statistically significant between the groups. Our findings imply that the theta responses at F3 location are highly instable in comparison to F4 in non-treated mild AD patients and cholinergic agents may modulate event related theta oscillatory activities in the frontal regions. P4-361
EFFECT OF VITAMIN B6 ON AGING-INDUCED CHANGES OF RAT BRAIN GLUTAMIC ACID DECARBOXYLASE ACTIVITY
Manouchehr Messripour, Islamic Azad University of Khorasgan, Isfahan, Iran (Islamic Republic of). Contact e-mail: [email protected] Background: Aging induces several alterations in brain structures and functions. Age-related changes in the synthesis and concentration of several neurotransmitters in the brain are described. A decrease in gammaaminobutyric acid (GABA) is also believed to contribute to Alzheimer’s disease (AD) development. GABA is major inhibitory neurotransmitter in the CNS, and its synthetic enzyme, glutamic acid decarboxylase (GAD), is thought to be a specific marker of CABAergic neurons. Objective(s): Because GAD is a pyridoxal phosphate -dependent enzyme, it is of interest to investigate the effect of vitamin B6 administration on adult and aged rat brain. Methods: Wistar rats (3 and 30 months old) were used. The animals were injected with 100 mg vitamin B6 /kg body weight /day (pyridoxine hydrochloride free base in saline) for 30 days. Control groups received saline only. In each experiment, five rats were killed by decapitation and brains were removed and homogenized in 10 ml ice- glass-cooled phosphate buffer (pH 7.4) .The homogenate was centrifuged at 70000g for 60 min at 4°C and the activity of GAD was measured in the supernatant by a Warburg manometeric method. The reaction was started by addition of glutamate into the supernatant (about 5 mg protein) at 37°C and CO2 production was measured for 15 min. Results: The specific activity of GAD in the brain supernatant of young and aged animals were 0.127 (SD⫽0.014) and 0.059 (SD⫽0.003) microlitre CO2 formation /min/ mg protein respectively. The differences were statistically significant (p⬍0.005). The lower brain GAD activity of aged animals is interpreted as being consistent with lower GABA production in aging brain. Administration of vitamin B6 increased the GAD activities in the brain supernatant of both young and aged animals by approximately 55 and 188% respectively. The vitamin B6 induced activation of GAD in aged rat brain was significantly higher than that of young animals. Conclusion: It is suggested that vitamin B6 may restore the brain GAD activity during aging. This suggestion is supported by the evidence that the putative role of GABA may be considered as a therapeutic target for controlling age-related symptomes. P4-362
THE EXPERIMENTAL RESEARCH ON NEUROTROPHIC EFFECT OF ANALOG OF APP 5-MER PEPTIDE
Shu Li Sheng, Jie Yao, Zhi Wei Zhao, Zhi Juan Ji, Neuro-Biochemistry Laboratory, Beijing Xuan-Wu Hospital, Capital University of Medical Sciences, Beijing, China. Contact e-mail: [email protected] Background: APP 17-mer peptide is an active fragment of amyloid precursor protein(APP). It could promote neuronal growth and differentiation, enhence axonal growth and differentiation. The peptide exerts its function by biding the cell surface through the domain containing RERMS sequence(APP328-332). Objective(s): APP328-332(APP
THETA OSCILLATIONS ARE PHASE LOCKED AFTER CHOLINESTERASE INHIBITORS IN FRONTAL AREA IN ALZHEIMER’S DISEASE
¨ niz2, Erol Bas¸ar2, Gorsev G. Yener1, Bahar A. Gu¨ntekin2, Adile T. O 1 Dokuz Eylul University; Departments of Neurology and Neural Sciences; Izmir, Multidisciplinary Brain Dynamics Research Center, Turkey; 2Dokuz Eylul University, Multidisciplinary Brain Dynamics ¨ BI˙TAK Brain Dynamics Research Unit, Research Center, Izmir, TU Ankara, Turkey. Contact e-mail: [email protected] Background: Theta oscillations are involved in mnemonic processes. Alzheimer’s disease (AD) is a common amnestic disorder. The current cholinesterase inhibitors may improve cognitive processes and affect the brain oscillations. Objective(s): To compare phase locking of theta event
S623
related oscillations in non-treated AD and treated AD and healthy elderly controls in frontal areas. Methods: Phase locking of theta (4-7Hz) event related oscillations of 22 mild probable AD patients (11 non-treated and 11 treated with cholinesterase inhibitors) and 20 healthy elderly controls were compared by using conventional visual oddball paradigm. Conclusions: At F3 location, theta responses of healthy subjects show stronger phase locking to stimulation than non-treated AD patients and the treated AD group has theta phase locking as strong as the control subjects. The F4 theta responses were not statistically significant between the groups. Our findings imply that the theta responses at F3 location are highly instable in comparison to F4 in non-treated mild AD patients and cholinergic agents may modulate event related theta oscillatory activities in the frontal regions. P4-361
EFFECT OF VITAMIN B6 ON AGING-INDUCED CHANGES OF RAT BRAIN GLUTAMIC ACID DECARBOXYLASE ACTIVITY
Manouchehr Messripour, Islamic Azad University of Khorasgan, Isfahan, Iran (Islamic Republic of). Contact e-mail: [email protected] Background: Aging induces several alterations in brain structures and functions. Age-related changes in the synthesis and concentration of several neurotransmitters in the brain are described. A decrease in gammaaminobutyric acid (GABA) is also believed to contribute to Alzheimer’s disease (AD) development. GABA is major inhibitory neurotransmitter in the CNS, and its synthetic enzyme, glutamic acid decarboxylase (GAD), is thought to be a specific marker of CABAergic neurons. Objective(s): Because GAD is a pyridoxal phosphate -dependent enzyme, it is of interest to investigate the effect of vitamin B6 administration on adult and aged rat brain. Methods: Wistar rats (3 and 30 months old) were used. The animals were injected with 100 mg vitamin B6 /kg body weight /day (pyridoxine hydrochloride free base in saline) for 30 days. Control groups received saline only. In each experiment, five rats were killed by decapitation and brains were removed and homogenized in 10 ml ice- glass-cooled phosphate buffer (pH 7.4) .The homogenate was centrifuged at 70000g for 60 min at 4°C and the activity of GAD was measured in the supernatant by a Warburg manometeric method. The reaction was started by addition of glutamate into the supernatant (about 5 mg protein) at 37°C and CO2 production was measured for 15 min. Results: The specific activity of GAD in the brain supernatant of young and aged animals were 0.127 (SD⫽0.014) and 0.059 (SD⫽0.003) microlitre CO2 formation /min/ mg protein respectively. The differences were statistically significant (p⬍0.005). The lower brain GAD activity of aged animals is interpreted as being consistent with lower GABA production in aging brain. Administration of vitamin B6 increased the GAD activities in the brain supernatant of both young and aged animals by approximately 55 and 188% respectively. The vitamin B6 induced activation of GAD in aged rat brain was significantly higher than that of young animals. Conclusion: It is suggested that vitamin B6 may restore the brain GAD activity during aging. This suggestion is supported by the evidence that the putative role of GABA may be considered as a therapeutic target for controlling age-related symptomes. P4-362
THE EXPERIMENTAL RESEARCH ON NEUROTROPHIC EFFECT OF ANALOG OF APP 5-MER PEPTIDE
Shu Li Sheng, Jie Yao, Zhi Wei Zhao, Zhi Juan Ji, Neuro-Biochemistry Laboratory, Beijing Xuan-Wu Hospital, Capital University of Medical Sciences, Beijing, China. Contact e-mail: [email protected] Background: APP 17-mer peptide is an active fragment of amyloid precursor protein(APP). It could promote neuronal growth and differentiation, enhence axonal growth and differentiation. The peptide exerts its function by biding the cell surface through the domain containing RERMS sequence(APP328-332). Objective(s): APP328-332(APP