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P.6.086 Dependence of semax long-lasting effects onthe time of its neonatal administration
P6, Ot,~er topics into halves by a transparent partition with holes permitting seeing next compartment and perceiving odors from it, but preventing any physical penetration. Through 5 days a receptive oestrus female of the same genotype was placed on the other side of the partition. The vision and smell of the female activate the male's behavior near the partition. Following are the behavioral indices measured during the first 10 min of the test: the number of approaches to the partition and the time spent near the partition. Further the partition was removed and next parameters of the male's behavioral reaction was registered during 20 min: the number of sniffing of the female and its duration, the number and duration of mounting's attempts. Statistical analysis was made by use of Spearmen's correlation method. Results and conclusions: Between values of the behavioral parameters near the partition and values of the physical contacts indices were obtained reliable positive correlation: the number of approaches the partition and the number of female sniffing (R=0.53, p=0.05); the time spent near the partition and the durations of sniffing (R=0.61, p=0.02), mounting's attempts (R=0.61, p=0.02) and its durations (R=0.55, p=0.04). The results are enough to consider the test is as adequate method for estimation of male mice sexual motivation. This modified 30 min behavioral test setting "-Femalebehind partition" allows evaluate intensity and stability in time sexual interest of male mice. The test is convenient and sensitive to alterations in male's sexual motivation during any kinds of stresses [1]. As shown recent pharmacological studies, the test can differentiates drugs on effective at initial and/or final phase of sexual motivation [2]. The work was supported by the RFBR (grant N 01-04-49402).
References [1] Amikishiwa A.V, Ovsyukova M.V. 2003. Effects of alternative social experience on sexual function of male mica Bull. Exper. Biol. and Mad. (Pus). 12, 686-689. [2] Amilcishiwa A.V. 2004. Contribution of GABA-A and B receptors in beginning and maintenance of sexual motivation in male mice. Proceeding of the 8th International Confa'ence ;%~ress and bdaaviouFh S~, Petersburg; Russia.
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Dependence of semax long-lasting effects on the time of its neonatal adminisb'ation
E.A. Sebentsova 1 , N.G. Levitskaya 1, L.A. Andreeva 1, L.Y. Alfeeva 1, N.E Myasoedo@, A.A. Kamensk'y 2, 17~st#ute
of Molecular Genetics 2?_45, Department of Chemistry of P~siological Actioe Substances, Moscow, Russian Federation; 2Lomonosoo Moscow State Unioers#y, Department of Human and Animal "pfrysiology, Moscow, IRusxian Federation A great amount of evidence has demonstrated that ACTK/MSHlike peptides have pronounced neurotrophic and neuroprotective effects on central and peripheral nervous systems. It has been shown that these peptides influence the developing nervous system during the first three weeks of life in the rat [1]. Heptapeptide Semax (MEI-WPGP) is the analogue of ACTH(4-10) with longlasting activity and great biological potency [2]. Studies on adult animals and humans have shown that this peptide possesses antihypoxic, antistroke and neurotrophic activity. Now the peptide is successfully used in treatment of patients with pathologies related to brain circulation dysfunction and with different intellectualmnestic problems of CNS. Our previous studies showed that Se~ neonatal administration on postnatal days 8-21 changed behaviour of rats at the age of 4 - 8 weeks. The aim of the present
S393
work was to study the behavioural effects of chronic Semax injections depending on the time of its neonatal administration. The work was carried out on white rat pups. Peptide (0,05 rrg/kg, i@) was injected daily during the first, second or second-third postnatal weeks. Control animals in the same nest received equal volume of saline on the same days. When the animals had reached the age of 1 month they were subjected to a series of test to measure their behavioural performance. It was shown that all animals treated with Semax during early postnatal life exhibited the decrease anxiety level in elevated plus-maze (on the 30 and 52 days of life) and improvement of passive avoidance behaviour (on the 39-42 days of life) in comparison to corresponding control. In addition, the rats received peptide injections during second or second-third weeks of life showed increased motor and exploratory activity on the 32, 50 and 56 days of life in open field test. Animals treated with Semax on the first postnatal week exhibited the decrease the behavioural parameters. Thus, long-term changes in rat behaviour induced by chronic Semax administration depend on the time of its neonatal injections. The data obtained allow to conclude that neonatal Semax administration modulate the development of nervous system. In addition we can suggest that peptide influence on the development of adaptive behaviour. The work was supported by Russian Found of 13aaio Research (Qrant 04-04-48511).
References [1] Ack= ~ B=a~ 4 ana S ~ a F~, P~ptiaes, 19S5, 6<2), 41-49, [2] Ashmarin IP, Necavibatko VII, Lwitskaya NG, et al Neurosci. Res. Commtm. 1995. 16. 105-112.
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Attempted suicide, psychopathology and genetic background - results of a family based association study in Hungary
S. Fekete j , P. Osvath j , P. Zill 2, B. Bondy 2, 1Unioersity of"pets, Medical Faculty, ,Psychiatry, Peos, Hungary; 2 Unioersi~2 of MunicK "psychiatry Cl, Neuroc~emiatry, MunicK Germaray Baekgroun& Suicidal behavior is a multiply determined act and it constitutes a heterogeneous group of behaviors, ranging from fatal violent attempts to attempts with low lethality rates, being usually impulsive. There is compelling evidence that suicidal behavior has a heritable component, although there seems to be a significant interaction between genes and environment in relation to the function of the serotonergic and noradrenergic system. The specific genes that contribute to suicide risk independently of associated psychiatric disorders are still unknown. Most studies have focused on genes of the serontonergic system as they are under partial genetic control and both suicidal behavior and impulsive aggression have been related to them (Mann, 2003). Thus genes coding for proteins of the serotonergic pathways are leading candidates for investigating possible associations. M e t h o & We studied three serotonergic gene polymorphisms (5-HTTLPR S/L, TPH A779C, 5-HT1B G861C) in a sample of 114 unrelated suicide attemptem (mainly non-violent methods), in 112 patients, 23 siblings and 56 healthy controls. Genotyping was done using polymerase chain reaction (PCR) based assa~. The primary analysis compared allde and genotype frequencies between patients and controls and tested for presence of linkage disequilibrium using the family based association test (FBAT). The genotypes were further related to clinical characteristics and to pwchological traits.
References [1] Amikishiwa A.V, Ovsyukova M.V. 2003. Effects of alternative social experience on sexual function of male mica Bull. Exper. Biol. and Mad. (Pus). 12, 686-689. [2] Amilcishiwa A.V. 2004. Contribution of GABA-A and B receptors in beginning and maintenance of sexual motivation in male mice. Proceeding of the 8th International Confa'ence ;%~ress and bdaaviouFh S~, Petersburg; Russia.
•
Dependence of semax long-lasting effects on the time of its neonatal adminisb'ation
E.A. Sebentsova 1 , N.G. Levitskaya 1, L.A. Andreeva 1, L.Y. Alfeeva 1, N.E Myasoedo@, A.A. Kamensk'y 2, 17~st#ute
of Molecular Genetics 2?_45, Department of Chemistry of P~siological Actioe Substances, Moscow, Russian Federation; 2Lomonosoo Moscow State Unioers#y, Department of Human and Animal "pfrysiology, Moscow, IRusxian Federation A great amount of evidence has demonstrated that ACTK/MSHlike peptides have pronounced neurotrophic and neuroprotective effects on central and peripheral nervous systems. It has been shown that these peptides influence the developing nervous system during the first three weeks of life in the rat [1]. Heptapeptide Semax (MEI-WPGP) is the analogue of ACTH(4-10) with longlasting activity and great biological potency [2]. Studies on adult animals and humans have shown that this peptide possesses antihypoxic, antistroke and neurotrophic activity. Now the peptide is successfully used in treatment of patients with pathologies related to brain circulation dysfunction and with different intellectualmnestic problems of CNS. Our previous studies showed that Se~ neonatal administration on postnatal days 8-21 changed behaviour of rats at the age of 4 - 8 weeks. The aim of the present
S393
work was to study the behavioural effects of chronic Semax injections depending on the time of its neonatal administration. The work was carried out on white rat pups. Peptide (0,05 rrg/kg, i@) was injected daily during the first, second or second-third postnatal weeks. Control animals in the same nest received equal volume of saline on the same days. When the animals had reached the age of 1 month they were subjected to a series of test to measure their behavioural performance. It was shown that all animals treated with Semax during early postnatal life exhibited the decrease anxiety level in elevated plus-maze (on the 30 and 52 days of life) and improvement of passive avoidance behaviour (on the 39-42 days of life) in comparison to corresponding control. In addition, the rats received peptide injections during second or second-third weeks of life showed increased motor and exploratory activity on the 32, 50 and 56 days of life in open field test. Animals treated with Semax on the first postnatal week exhibited the decrease the behavioural parameters. Thus, long-term changes in rat behaviour induced by chronic Semax administration depend on the time of its neonatal injections. The data obtained allow to conclude that neonatal Semax administration modulate the development of nervous system. In addition we can suggest that peptide influence on the development of adaptive behaviour. The work was supported by Russian Found of 13aaio Research (Qrant 04-04-48511).
References [1] Ack= ~ B=a~ 4 ana S ~ a F~, P~ptiaes, 19S5, 6<2), 41-49, [2] Ashmarin IP, Necavibatko VII, Lwitskaya NG, et al Neurosci. Res. Commtm. 1995. 16. 105-112.
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Attempted suicide, psychopathology and genetic background - results of a family based association study in Hungary
S. Fekete j , P. Osvath j , P. Zill 2, B. Bondy 2, 1Unioersity of"pets, Medical Faculty, ,Psychiatry, Peos, Hungary; 2 Unioersi~2 of MunicK "psychiatry Cl, Neuroc~emiatry, MunicK Germaray Baekgroun& Suicidal behavior is a multiply determined act and it constitutes a heterogeneous group of behaviors, ranging from fatal violent attempts to attempts with low lethality rates, being usually impulsive. There is compelling evidence that suicidal behavior has a heritable component, although there seems to be a significant interaction between genes and environment in relation to the function of the serotonergic and noradrenergic system. The specific genes that contribute to suicide risk independently of associated psychiatric disorders are still unknown. Most studies have focused on genes of the serontonergic system as they are under partial genetic control and both suicidal behavior and impulsive aggression have been related to them (Mann, 2003). Thus genes coding for proteins of the serotonergic pathways are leading candidates for investigating possible associations. M e t h o & We studied three serotonergic gene polymorphisms (5-HTTLPR S/L, TPH A779C, 5-HT1B G861C) in a sample of 114 unrelated suicide attemptem (mainly non-violent methods), in 112 patients, 23 siblings and 56 healthy controls. Genotyping was done using polymerase chain reaction (PCR) based assa~. The primary analysis compared allde and genotype frequencies between patients and controls and tested for presence of linkage disequilibrium using the family based association test (FBAT). The genotypes were further related to clinical characteristics and to pwchological traits.