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P77 Nyctanthes arbor tristis extract restores the cartilage damage during experimental arthritis
174
Indian Journal of Rheumatology 2006 December; Vol. 1, No. 3
Posters
Methods: The second generation ELISA kit was used to determine anti-CCP. Controls–healthy (HC) = 114; Bhigwan undifferentiated arthritis (IA-U) = 102; Pune referral RA (ACR) = 150. RF (nephelometry) Cut-off: 40 IU/ml. Multivariable linear and logistic regressions were done. Results: Table 1 Sensitivity and specificity for anti-CCP Bhigwan RA (n = 42) Sensitivity 62% Sepcificity1 98% Specificity2 96%
Referral RA (n = 150) 84% 98% 96%
1. Using healthy controls1; and Using patients with IA-U2. 2. 71% rural seropositive tested anti-CCP positive. 65% and 29% of rural incidence RA patients were positive for anti-CCP and RF, respectively.
Conclusion: This study demonstrates the sensitivity and specificity of anti-CCP primarily from a population study and further endorses its diagnostic value. Acknowledgments: Sponsored by CRD: R&D Lab and Arthritis Research and Care Foundation.
P77
Nyctanthes arbor tristis extract restores the cartilage damage during experimental arthritis B Rathore, AA Mahdi, BN Paul1, PN Saxena2, SK Das3 Department of Biochemistry and Rheumatology3, King George’s Medical University, Lucknow, India, 1Immunobiology Laboratory, Industrial Toxicology Research Centre, MG Marg, Lucknow, India, 2Department of Zoology, Dr. B R Ambedkar University, Agra, India.
Introduction: The pathogenesis of rheumatoid arthritis is unclear but activated synovial fibroblasts appear to play a major role in disease. It is speculated that their ability to induce and perpetuate inflammation is associated with the production of pro-inflammatory cytokines which in turn causes inflamed synovium. Objectives: To evaluate the therapeutic effect of a herbal extract in an experimental model of rheumatoid arthritis. Methods: Arthritis was induced in mice by injecting the Freund’s Complete Adjuvant in the foot pad. A booster dose was given on the day 12th. Nyctanthes arbor tristis extract was administered orally from the day of induction of disease. On the day 47th animals were sacrificed and the ankle joint was processed for the histopathology. Results: Infiltration of inflammatory cells, neovascularisation and cartilage damage was found negligible in the extract treated animals as compared to the arthritic one. The cell lining was also observed intact in Nyctanthes arbor tristis extract treated animals as compared to the control animals. Conclusion: Our findings open up a new window to explore and scientifically evaluate herbal therapeutic approach for rheumatoid arthritis.
Indian Journal of Rheumatology 2006 December; Vol. 1, No. 3
Posters
Methods: The second generation ELISA kit was used to determine anti-CCP. Controls–healthy (HC) = 114; Bhigwan undifferentiated arthritis (IA-U) = 102; Pune referral RA (ACR) = 150. RF (nephelometry) Cut-off: 40 IU/ml. Multivariable linear and logistic regressions were done. Results: Table 1 Sensitivity and specificity for anti-CCP Bhigwan RA (n = 42) Sensitivity 62% Sepcificity1 98% Specificity2 96%
Referral RA (n = 150) 84% 98% 96%
1. Using healthy controls1; and Using patients with IA-U2. 2. 71% rural seropositive tested anti-CCP positive. 65% and 29% of rural incidence RA patients were positive for anti-CCP and RF, respectively.
Conclusion: This study demonstrates the sensitivity and specificity of anti-CCP primarily from a population study and further endorses its diagnostic value. Acknowledgments: Sponsored by CRD: R&D Lab and Arthritis Research and Care Foundation.
P77
Nyctanthes arbor tristis extract restores the cartilage damage during experimental arthritis B Rathore, AA Mahdi, BN Paul1, PN Saxena2, SK Das3 Department of Biochemistry and Rheumatology3, King George’s Medical University, Lucknow, India, 1Immunobiology Laboratory, Industrial Toxicology Research Centre, MG Marg, Lucknow, India, 2Department of Zoology, Dr. B R Ambedkar University, Agra, India.
Introduction: The pathogenesis of rheumatoid arthritis is unclear but activated synovial fibroblasts appear to play a major role in disease. It is speculated that their ability to induce and perpetuate inflammation is associated with the production of pro-inflammatory cytokines which in turn causes inflamed synovium. Objectives: To evaluate the therapeutic effect of a herbal extract in an experimental model of rheumatoid arthritis. Methods: Arthritis was induced in mice by injecting the Freund’s Complete Adjuvant in the foot pad. A booster dose was given on the day 12th. Nyctanthes arbor tristis extract was administered orally from the day of induction of disease. On the day 47th animals were sacrificed and the ankle joint was processed for the histopathology. Results: Infiltration of inflammatory cells, neovascularisation and cartilage damage was found negligible in the extract treated animals as compared to the arthritic one. The cell lining was also observed intact in Nyctanthes arbor tristis extract treated animals as compared to the control animals. Conclusion: Our findings open up a new window to explore and scientifically evaluate herbal therapeutic approach for rheumatoid arthritis.