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P.8.086 The effects of chronic psychological stress on carbohydrate metabolism in rat
P8 Other topics
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Comparison of four different antipsychotics on MK-801-induced hyperlocomotion in mice
Y. Su 1 *, T.M. Si 2. ]htstitute of Mental Health, Peking University,
China, Department of Psychopharmalolgy, Beijing, China; 2htstitute of Mental Health, Peking University, China, Department of Psychopharmacology, China P u r p o s e of the study: To compare the effects of four antipsy-
chotics on hyperlocomotion induced by MK-801. Materials a n d methods: Animals: Male Kunming mice, weighing 20 25 g at the time of testing. Drugs and drug administration: (+)-MK-801 hydrogen maleate was dissolved in physical saline. Haloperidol, risperidone, clozapine were dissolved in approximately 5 microliter acetic acid and diluted with glucose (5%) to the desired concentration. Quetiapine was dissolved in 0.1N hydrochloric acid and diluted with saline. The antagonists were all given 5 min before MK-801 administration. All substances were given intraperitoneally in a volume of 10 ml/kg. The selected MK-801 dose of 0.25mg/kg was based on previous reports and preliminary test. Apparatus: Locomotor activity was measured by an automated video tracking system. The system consisted of four activity chambers (25 × 25 × 40 cm), a video collection card, a computer and analysis software. The total distances of mice in a selected period could be computed via software. After drug administration, mice were immediately put into the test chambers to record the locomotor activity for 90min. Results: Haloperidol (0.1, 0.3mg/kg), risperidone (0.025, 0.050, 0.075mg/kg), clozapine (1.0, 1.5, 2.0mg/kg) all reduced MK-801-induced hyperactivity. Haloperidol did so only at a dose (0.1 mg/kg) that also decreased spontaneous activity, whereas risperidone and clozapine reduced MK-801-induced hyperactivity at a lower dose that had no effect on spontaneous activity (0.025 mg/kg and 1.0 mg/kg respectively). According to timecourse effect curve, the latency of peak levels of hyperlocomotion by MK-801 (0.25mg/kg) was delayed by quetiapine (2.5, 5.0mg/kg), but total distances in 90min period were not reduced. Lower dose of clozapine (1.0mg/kg) antagonized MK801-induced hyperlocomotion especially during the 0 30 minute time interval. Lower dose of haloperidol (0.1 mg/kg) inhibited hyperlocomotion especially during the 60 90 minute time interval. Lower dose of risperidone (0.025mg/kg) could reduce hyperacitivity in the whole 90 minute test period. Conclusions: The effects of four different antipsychotic agents on MK-801-induced hyperlocomotion are different which may be involved with different pharmacological mechanisms of them and other time-by-drug interaction factors. Risperidone is more effective than other antipsychotics on the MK-801-induced hyperlocomotion.
References [1] Nisson, M., Waters, S., Waters N., et al. 2001. A behavioural pattern analysis of hypoglutamatergic mice effects of four different antipsychotic agents. J Neural Transm. 108:1181 1196. [2] Verma A., Kulkarni S.K. 1992. Modulation of MK-801 response by dopaminergic agents in mice. Psychopharmacology 107: 431436. [3] Olney J.W., Newcomer J.W., Farber N.B. 1999. NMDA receptor hypofunction model of schizophrenia. J Psychiatr Res 33:523 533.
~ T h e
$643
effects of chronic psychological stress on carbohydrate metabolism in rat
H. Zardouz*. Shaheed Beheshti University of Medical Sciences, Neuroscience Research Centre, Tehran, Islamic Republic of Iran
Several studies have shown that stress has major effects on carbohydrate metabolism. There are also some evidences suggesting that stress may induce type 1 and type 2 diabetes mellitus in animals and also human subjects. The present study has investigated the role of chronic psychological stress on carbohydrate metabolism in male rats. Animals exposed to different stressors twice daily for 1 h. Blood samples were taken on 1st, 15th and 30th days of experimental period by tail snipping, then oral glucose tolerance test (OGTT) was done. Stressed rats did not gain weight compared to controls. Fasting plasma glucose levels on 15th and 30th days showed significant increase in stressed rats compared with the controls. The plasma levels of glucose after performing oral glucose tolerance test was significantly higher on 15th and 30th days of experiment in stressed group. Fasting plasma insulin showed significant decrease on 15th and 30th days of experiment in stressed rats. Similar reduction in the plasma levels of insulin was observed following oral glucose tolerance test in stressed rats. The results show that psychological stress can affect glucose metabolism and this effect may be accompanied by a reduction in plasma insulin levels.
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Quality of life and subthreshold affective symptomatology
A. Goracci*, M. Martinucci, U. Scalcione, C. Sbaragli, R Castrogiovanni. University of Siena, Neuroscience Division of
Psychiatry, Siena, Italy Introduction: Quality of Life (QoL) in patients affected by psy-
chiatric disorders is widely investigated in several recent studies; but there are few studies about the relationships between quality of life and subthreshold psychiatric symptomatology (Goracci A, 2005). According to the more and more frequently application of mood and anxiety spectrum model, we used the Structured Clinical Interview for Bipolar and Anxiety Spectrum to assess subthreshold symptomatology. The aim of this study was to correlate bipolar and anxiety spectrum with quality of life in a sample of healthy and drug-free subjects. M e t h o d s : A sample of 233 physically healthy subjects, without psychiatric disorders, was recruited in the general population; their ages ranged from 22 55 years of age, with a mean of 33.5. Patients rated themselves on the "Quality of Life Enjoyment and Satisfaction Questionnaire" (Q-LES-Q) and the "Structured Clinical Interview for Bipolar Spectrum" (MOODS-SR) (Cassano et al., 1999); 44 of them filled the questionnaires for panicagoraphobic spectrum (PAS-SR), social-phobic spectrum (SHYSR) and obsessive-compulsive spectrum (OBS-SR). Results: We found significant negative correlations between the "Physical Health" domain of Q-LES-Q and the PAS-SR and the MOODS-SR (p < 0.05; p < 0.01); the "Subjective Feeling" domain of Q-LES-Q was negatively correlated with the PAS-SR, the SHYSR and the MOODS-SR, especially with the area referred to depressive symptoms (p <0.05; p <0.01, p <0.01); the "Work" domain was negatively correlated with the SHY-SR and the MOODS-SR, especially with the area referred to depressive symptoms (p < 0.01; p < 0.05); the "School/Course Work" domain with
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Comparison of four different antipsychotics on MK-801-induced hyperlocomotion in mice
Y. Su 1 *, T.M. Si 2. ]htstitute of Mental Health, Peking University,
China, Department of Psychopharmalolgy, Beijing, China; 2htstitute of Mental Health, Peking University, China, Department of Psychopharmacology, China P u r p o s e of the study: To compare the effects of four antipsy-
chotics on hyperlocomotion induced by MK-801. Materials a n d methods: Animals: Male Kunming mice, weighing 20 25 g at the time of testing. Drugs and drug administration: (+)-MK-801 hydrogen maleate was dissolved in physical saline. Haloperidol, risperidone, clozapine were dissolved in approximately 5 microliter acetic acid and diluted with glucose (5%) to the desired concentration. Quetiapine was dissolved in 0.1N hydrochloric acid and diluted with saline. The antagonists were all given 5 min before MK-801 administration. All substances were given intraperitoneally in a volume of 10 ml/kg. The selected MK-801 dose of 0.25mg/kg was based on previous reports and preliminary test. Apparatus: Locomotor activity was measured by an automated video tracking system. The system consisted of four activity chambers (25 × 25 × 40 cm), a video collection card, a computer and analysis software. The total distances of mice in a selected period could be computed via software. After drug administration, mice were immediately put into the test chambers to record the locomotor activity for 90min. Results: Haloperidol (0.1, 0.3mg/kg), risperidone (0.025, 0.050, 0.075mg/kg), clozapine (1.0, 1.5, 2.0mg/kg) all reduced MK-801-induced hyperactivity. Haloperidol did so only at a dose (0.1 mg/kg) that also decreased spontaneous activity, whereas risperidone and clozapine reduced MK-801-induced hyperactivity at a lower dose that had no effect on spontaneous activity (0.025 mg/kg and 1.0 mg/kg respectively). According to timecourse effect curve, the latency of peak levels of hyperlocomotion by MK-801 (0.25mg/kg) was delayed by quetiapine (2.5, 5.0mg/kg), but total distances in 90min period were not reduced. Lower dose of clozapine (1.0mg/kg) antagonized MK801-induced hyperlocomotion especially during the 0 30 minute time interval. Lower dose of haloperidol (0.1 mg/kg) inhibited hyperlocomotion especially during the 60 90 minute time interval. Lower dose of risperidone (0.025mg/kg) could reduce hyperacitivity in the whole 90 minute test period. Conclusions: The effects of four different antipsychotic agents on MK-801-induced hyperlocomotion are different which may be involved with different pharmacological mechanisms of them and other time-by-drug interaction factors. Risperidone is more effective than other antipsychotics on the MK-801-induced hyperlocomotion.
References [1] Nisson, M., Waters, S., Waters N., et al. 2001. A behavioural pattern analysis of hypoglutamatergic mice effects of four different antipsychotic agents. J Neural Transm. 108:1181 1196. [2] Verma A., Kulkarni S.K. 1992. Modulation of MK-801 response by dopaminergic agents in mice. Psychopharmacology 107: 431436. [3] Olney J.W., Newcomer J.W., Farber N.B. 1999. NMDA receptor hypofunction model of schizophrenia. J Psychiatr Res 33:523 533.
~ T h e
$643
effects of chronic psychological stress on carbohydrate metabolism in rat
H. Zardouz*. Shaheed Beheshti University of Medical Sciences, Neuroscience Research Centre, Tehran, Islamic Republic of Iran
Several studies have shown that stress has major effects on carbohydrate metabolism. There are also some evidences suggesting that stress may induce type 1 and type 2 diabetes mellitus in animals and also human subjects. The present study has investigated the role of chronic psychological stress on carbohydrate metabolism in male rats. Animals exposed to different stressors twice daily for 1 h. Blood samples were taken on 1st, 15th and 30th days of experimental period by tail snipping, then oral glucose tolerance test (OGTT) was done. Stressed rats did not gain weight compared to controls. Fasting plasma glucose levels on 15th and 30th days showed significant increase in stressed rats compared with the controls. The plasma levels of glucose after performing oral glucose tolerance test was significantly higher on 15th and 30th days of experiment in stressed group. Fasting plasma insulin showed significant decrease on 15th and 30th days of experiment in stressed rats. Similar reduction in the plasma levels of insulin was observed following oral glucose tolerance test in stressed rats. The results show that psychological stress can affect glucose metabolism and this effect may be accompanied by a reduction in plasma insulin levels.
•
Quality of life and subthreshold affective symptomatology
A. Goracci*, M. Martinucci, U. Scalcione, C. Sbaragli, R Castrogiovanni. University of Siena, Neuroscience Division of
Psychiatry, Siena, Italy Introduction: Quality of Life (QoL) in patients affected by psy-
chiatric disorders is widely investigated in several recent studies; but there are few studies about the relationships between quality of life and subthreshold psychiatric symptomatology (Goracci A, 2005). According to the more and more frequently application of mood and anxiety spectrum model, we used the Structured Clinical Interview for Bipolar and Anxiety Spectrum to assess subthreshold symptomatology. The aim of this study was to correlate bipolar and anxiety spectrum with quality of life in a sample of healthy and drug-free subjects. M e t h o d s : A sample of 233 physically healthy subjects, without psychiatric disorders, was recruited in the general population; their ages ranged from 22 55 years of age, with a mean of 33.5. Patients rated themselves on the "Quality of Life Enjoyment and Satisfaction Questionnaire" (Q-LES-Q) and the "Structured Clinical Interview for Bipolar Spectrum" (MOODS-SR) (Cassano et al., 1999); 44 of them filled the questionnaires for panicagoraphobic spectrum (PAS-SR), social-phobic spectrum (SHYSR) and obsessive-compulsive spectrum (OBS-SR). Results: We found significant negative correlations between the "Physical Health" domain of Q-LES-Q and the PAS-SR and the MOODS-SR (p < 0.05; p < 0.01); the "Subjective Feeling" domain of Q-LES-Q was negatively correlated with the PAS-SR, the SHYSR and the MOODS-SR, especially with the area referred to depressive symptoms (p <0.05; p <0.01, p <0.01); the "Work" domain was negatively correlated with the SHY-SR and the MOODS-SR, especially with the area referred to depressive symptoms (p < 0.01; p < 0.05); the "School/Course Work" domain with