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P838 Features of lipid peroxidation and antioxidant status of patients with climacteric syndrome
Poster presentations / International Journal of Gynecology & Obstetrics 107S2 (2009) S413–S729
P838 Features of lipid peroxidation and antioxidant status of patients with climacteric syndrome N. Podgornova1 , R. Chandra-D’Mello2 , G. Grechkanev1 . 1 Nizhegorod State Medical Academy, Nizhny Novgorod, Russia, 2 Dr. Rajani’s Clinic, Baku, Azerbaijan Aim: To study the status of lipid peroxidation (LP) and antioxidant defense system (AODS) in patients with climacteric syndrome. Material and Methods: We examined 40 patients with climacteric syndrome. For initial assessment of the intensity of free radical oxidation we used the screening method with induced chemiluminescence of the serum. As activators we used a 0.05 M solution of ferrous sulphate and a 2% solution of hydrogen peroxide. Measurement of intensity of the luminescence in 30 seconds was carried out by measuring indicators such as Imax in mV/sec, S in mV/sec, tg 2a. We also investigated the levels of molecular products of LP viz. diene conjugates (DC), triene conjugates (TC), Schiff bases (SB) by spectrophotometry. Activity of antioxidant enzymes such as catalase and superoxide dismutase were also assayed by spectrophotometry. Results: The data revealed that indicators of free-radical oxidation – Imax and S, measured by induced chemiluminescence, initially differed from normal for 60% of the patients. Thus the indicator Imax, which reflects the activity of POL, was equal to 2.56±0.17 mV/sec (the normal range being 1.5–2.0 mV/sec). Indicator S also was raised at 16.03±0.02 mv/sec (normal range 12.5–15.5 mV/sec). The indicator tg 2a was on at level 0.59±0.01 (normal range – 0.28–0.55). The quantitative analysis of molecular products of LP has revealed that levels of primary products – DC & TC have been moderately raised, and those of final products – SB were unchanged. The levels of enzymes possessing antioxidant activity were lowered. Conclusion: Our study reveals that the homeostasis of patients with climacteric syndrome is deranged primarily due to hyperactive lipoperoxidase and lowered potential of AODS. Such a combination is favorable for the progression of the disease and hence requires pathogenically justified treatment. P839 Several risk factors in relation to endometrial adenocarcinoma diagnosis features V. Antovska1 , G. Dimitrov2 , A. Stefanija3 , G. Kiproska4 , T. Nikolova5 . 1 Department of Urogynecology, University Clinic of Gyn and Obst., Skopje, 2 University Clinic of Gynecology and Obstetrics, Skopje, 3 University Clinic of Gynecology and Obstetrics, Skopje, 4 University Clinic of Gynecology and Obstetrics, Skopje, 5 doctor on Specialisation Both endogenous and exogenous estrogen high levels and the events inducing or counteracting these have been related to endometrial cancer risk; nevertheless their realationship to certain aspects of the endometrial cancer diagnosis features could still be debated. The aim of this study is to investigate the relatinship between some clinical signs having risk factor significance, as duration of the abnormal uterine bleeding, age and menarche, menopausal age, parity degree and certain diagnostic aspects as age at diagnosis, FIGO stage disease, histological grade. Our population-based study included 40 aged 40–78 years (aveage 58.9 years diagnosed and sugical treated between 2005–2008 for endometrial adenocarcinoma. 6 patients were not menopaused. Data from the medical files was used. The statistical analysis used the calculation of the correlation coefficient (r). Our results showed the existence of a strong correlation between the parity degree and the patients age at the moment of diagnosis (r = 0.80), a medium correlation between the duration of the abnormal uterine bleeding and both FIGO surgical stage (r = 0.52) and histological grade (r = 0.61), as well as between the age at menarche and the patients age at diagnosis(r = 0.59). We found no correlation between
S651
the menopausal age and the patients age at diagnosis9 (r = 0.18).The results are cosistent with the idea that the accepted risk factors are of different importance that must be carefully assessed together with prognostic factors. P840 The effects of red clover derived isoflavones on serum lipids, bone turnover and bone density in postmenopausal women J. Dotlic1 , M. Terzic1 , T. Mihailovic2 , S. Maricic3 . 1 Department of Ob/Gyn, School of Medicine, University of Belgrade, 11000 Belgrade, Visegradska 26, Serbia, 2 Ultramedica Clinic, 11000 Belgrade, Vojvode Dobrnjca 34, Serbia, 3 General Health Center “Savski Venac”, 11000 Belgrade, Pasterova 1, Serbia Objectives: The aim of the study was to investigate the effects of red clover derived isoflavones on serum lipid levels and biochemical markers of bone turnover and bone mineral density in postmenopausal women. Materials and Methods: The study involved 53 healthy postmenopausal women, divided in two groups. The first group of 29 women received red clover derived isoflavones while the second, control group of 24 women was without medications. We investigated total cholesterol, cholesterol fractions and triglycerides in blood of patients in both groups, before treatment and in four months periods throughout 12 months. The effects on bone structure were assessed by the level of bone composing markers (osteocalcine, alkaline phosphatase), evaluated prior to therapy initiation and in six months periods throughout one year, and by osteodensitometry which was performed twice: before isoflavone commencement and after twelve months. Results: Both total serum cholesterol and LDL cholesterol, as well as the level of triglycerides, were decreased significantly in the group receiving phytoestrogens. On the other hand, HDL cholesterol level showed a significant increase. During phytoestrogen intake, a significant increase of both bone composition markers and osteodensity was observed. Conclusions: Red clover phytoestrogen supplementation in postmenopausal women showed favorable metabolic effects on serum lipids. Furthermore, these isoflavones exerted positive effects on bone structure and bone turnover, thus preventing the progression of osteoporosis. P841 Evaluation of the quality of life of women using an estradiol and drospirenone combined oral HRT G. Galazios1 , E. Kalamara1 , P. Tsikouras1 , A. Dafopoulos1 , V. Liberis1 , G. Kolios2 . 1 Dept Ob/Gyn Duth, 2 Dept of Farmacology Duth Objective: The aim of this study was to estimate the beneficial effects of drospirenone/estradiol combination, in postmenopausal women, concerning the improvement of Quality of Life (QoL). Study: A non-comparative follow-up study was conducted in the Dept of O/G, Medical School, DUTH. 61 women took part in the study after consent. All of them were up to 1 year menopausal, presented climasteric symptoms, had intact uterus and present no contraindications. A drospirenone (2 mg) and estradiol (1 mg) combination (HRT) was given to all of them. Women completed a form, containing demographic and symptoms’ characteristics and had a follow up at 3 and 6 months after inclusion. The main outcome of the study was the QoL, assessed by means of Women’ Health Questionnaire (WHQ). WHQ’s factors were compared through different time points using non-parametric Friedman’s test for paired samples. Categorical data were compared using Mc Newmar’s test. Results: Response rate for the WHQ was 100% at baseline and 3 months later and 97% at the 6 months visit. According to patients and physicians, tolerability and therapeutic success of HRT was excellent for all of them (n = 61) and almost all (n = 60) respectively. Most common symptoms were headache, breast tenderness and
P838 Features of lipid peroxidation and antioxidant status of patients with climacteric syndrome N. Podgornova1 , R. Chandra-D’Mello2 , G. Grechkanev1 . 1 Nizhegorod State Medical Academy, Nizhny Novgorod, Russia, 2 Dr. Rajani’s Clinic, Baku, Azerbaijan Aim: To study the status of lipid peroxidation (LP) and antioxidant defense system (AODS) in patients with climacteric syndrome. Material and Methods: We examined 40 patients with climacteric syndrome. For initial assessment of the intensity of free radical oxidation we used the screening method with induced chemiluminescence of the serum. As activators we used a 0.05 M solution of ferrous sulphate and a 2% solution of hydrogen peroxide. Measurement of intensity of the luminescence in 30 seconds was carried out by measuring indicators such as Imax in mV/sec, S in mV/sec, tg 2a. We also investigated the levels of molecular products of LP viz. diene conjugates (DC), triene conjugates (TC), Schiff bases (SB) by spectrophotometry. Activity of antioxidant enzymes such as catalase and superoxide dismutase were also assayed by spectrophotometry. Results: The data revealed that indicators of free-radical oxidation – Imax and S, measured by induced chemiluminescence, initially differed from normal for 60% of the patients. Thus the indicator Imax, which reflects the activity of POL, was equal to 2.56±0.17 mV/sec (the normal range being 1.5–2.0 mV/sec). Indicator S also was raised at 16.03±0.02 mv/sec (normal range 12.5–15.5 mV/sec). The indicator tg 2a was on at level 0.59±0.01 (normal range – 0.28–0.55). The quantitative analysis of molecular products of LP has revealed that levels of primary products – DC & TC have been moderately raised, and those of final products – SB were unchanged. The levels of enzymes possessing antioxidant activity were lowered. Conclusion: Our study reveals that the homeostasis of patients with climacteric syndrome is deranged primarily due to hyperactive lipoperoxidase and lowered potential of AODS. Such a combination is favorable for the progression of the disease and hence requires pathogenically justified treatment. P839 Several risk factors in relation to endometrial adenocarcinoma diagnosis features V. Antovska1 , G. Dimitrov2 , A. Stefanija3 , G. Kiproska4 , T. Nikolova5 . 1 Department of Urogynecology, University Clinic of Gyn and Obst., Skopje, 2 University Clinic of Gynecology and Obstetrics, Skopje, 3 University Clinic of Gynecology and Obstetrics, Skopje, 4 University Clinic of Gynecology and Obstetrics, Skopje, 5 doctor on Specialisation Both endogenous and exogenous estrogen high levels and the events inducing or counteracting these have been related to endometrial cancer risk; nevertheless their realationship to certain aspects of the endometrial cancer diagnosis features could still be debated. The aim of this study is to investigate the relatinship between some clinical signs having risk factor significance, as duration of the abnormal uterine bleeding, age and menarche, menopausal age, parity degree and certain diagnostic aspects as age at diagnosis, FIGO stage disease, histological grade. Our population-based study included 40 aged 40–78 years (aveage 58.9 years diagnosed and sugical treated between 2005–2008 for endometrial adenocarcinoma. 6 patients were not menopaused. Data from the medical files was used. The statistical analysis used the calculation of the correlation coefficient (r). Our results showed the existence of a strong correlation between the parity degree and the patients age at the moment of diagnosis (r = 0.80), a medium correlation between the duration of the abnormal uterine bleeding and both FIGO surgical stage (r = 0.52) and histological grade (r = 0.61), as well as between the age at menarche and the patients age at diagnosis(r = 0.59). We found no correlation between
S651
the menopausal age and the patients age at diagnosis9 (r = 0.18).The results are cosistent with the idea that the accepted risk factors are of different importance that must be carefully assessed together with prognostic factors. P840 The effects of red clover derived isoflavones on serum lipids, bone turnover and bone density in postmenopausal women J. Dotlic1 , M. Terzic1 , T. Mihailovic2 , S. Maricic3 . 1 Department of Ob/Gyn, School of Medicine, University of Belgrade, 11000 Belgrade, Visegradska 26, Serbia, 2 Ultramedica Clinic, 11000 Belgrade, Vojvode Dobrnjca 34, Serbia, 3 General Health Center “Savski Venac”, 11000 Belgrade, Pasterova 1, Serbia Objectives: The aim of the study was to investigate the effects of red clover derived isoflavones on serum lipid levels and biochemical markers of bone turnover and bone mineral density in postmenopausal women. Materials and Methods: The study involved 53 healthy postmenopausal women, divided in two groups. The first group of 29 women received red clover derived isoflavones while the second, control group of 24 women was without medications. We investigated total cholesterol, cholesterol fractions and triglycerides in blood of patients in both groups, before treatment and in four months periods throughout 12 months. The effects on bone structure were assessed by the level of bone composing markers (osteocalcine, alkaline phosphatase), evaluated prior to therapy initiation and in six months periods throughout one year, and by osteodensitometry which was performed twice: before isoflavone commencement and after twelve months. Results: Both total serum cholesterol and LDL cholesterol, as well as the level of triglycerides, were decreased significantly in the group receiving phytoestrogens. On the other hand, HDL cholesterol level showed a significant increase. During phytoestrogen intake, a significant increase of both bone composition markers and osteodensity was observed. Conclusions: Red clover phytoestrogen supplementation in postmenopausal women showed favorable metabolic effects on serum lipids. Furthermore, these isoflavones exerted positive effects on bone structure and bone turnover, thus preventing the progression of osteoporosis. P841 Evaluation of the quality of life of women using an estradiol and drospirenone combined oral HRT G. Galazios1 , E. Kalamara1 , P. Tsikouras1 , A. Dafopoulos1 , V. Liberis1 , G. Kolios2 . 1 Dept Ob/Gyn Duth, 2 Dept of Farmacology Duth Objective: The aim of this study was to estimate the beneficial effects of drospirenone/estradiol combination, in postmenopausal women, concerning the improvement of Quality of Life (QoL). Study: A non-comparative follow-up study was conducted in the Dept of O/G, Medical School, DUTH. 61 women took part in the study after consent. All of them were up to 1 year menopausal, presented climasteric symptoms, had intact uterus and present no contraindications. A drospirenone (2 mg) and estradiol (1 mg) combination (HRT) was given to all of them. Women completed a form, containing demographic and symptoms’ characteristics and had a follow up at 3 and 6 months after inclusion. The main outcome of the study was the QoL, assessed by means of Women’ Health Questionnaire (WHQ). WHQ’s factors were compared through different time points using non-parametric Friedman’s test for paired samples. Categorical data were compared using Mc Newmar’s test. Results: Response rate for the WHQ was 100% at baseline and 3 months later and 97% at the 6 months visit. According to patients and physicians, tolerability and therapeutic success of HRT was excellent for all of them (n = 61) and almost all (n = 60) respectively. Most common symptoms were headache, breast tenderness and