- Email: [email protected]
Parallel polynucleotide sequencing method (WH Wong, US)
DNA sequence of the invention, an expression vector comprising the DNA sequence of the invention, and a CBDlenzyme hybrid.
Fermentation r Preparation of D-amino acids by direct fermentative means (IG Fotheringham et al, US)
Monsanto Co N” WO 98114602. PCT. The present invention relates to materials and method for production of natural and unnatural D-amino acids. In particular, the present invention relates to a fermentation method for the production of D-amino acids using recombinant host cells. (...)
Biopesticides > Biological insect control agents expressing insectspecific mite toxin genes, methods and compositions (L Miller et a/, US)
University of Georgia Research Foundation Inc and American Cyanamid Co N” WO 98114578, PCT. Provided herein are genetically engineered baculoviruses which express insectspecific toxins, preferably paralytic neurotoxins, under the regulatory control of strong promoters expressed early after infection and in a wide variety of insect cell. (...I
> Process for biological removal of sulphide (AJ Janssen and CJ Buisman, NL)
Thiopaq Sulfur Systems BV N” 845288, EP. The invention provides a process for the removal of sulphides from a gas stream by scrubbing the gas with an aqueous washing liquid and treating the washing liquid with sulphide-oxidising bacteria in the presence of an electron acceptor and reusing the treated liquid as a washing liquid, wherein the scrubbing and the bacterial treatment are carried out in the same reactor and nitrate is used as the electron acceptor. The process is especially useful for desulphurising natural gas at high pressure. 50 BIOFUTUR181 l Septembre1998
> Microbiological method for eliminating halogenated ethenes (P Koziollek et al, DE)
Fraunhofer-Gesellschaft zur FGrderung der Angewandten Forschung EV and the inventors N” WO 98/l 5338, PCT. The invention relates to microbiological elimination of halogenated ethenes. Said method is characterized by the fact that bacteria strains which degrade halogenated ethene and grow with ethene as a source of carbon and energy are preadapted with ethene as a source of carbon and energy for reformation and for energy supply and broughi into contact with the halogenated ethenes to be eliminated in an aerobic bioreactor. > A Rhodococcus reductase and its desulfurization of fuel (KA Gray et al,
flavin use in fossil US)
Energy Biosystems Corp N” WO 98/l 7787, PCT. The invention provides a novel flavin reductase, which has been isolated from Rhodococcus strain sp. IGTS8 and purified to homogeneity. The flavin reductase is of use in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds. The method comprises the steps of: contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of the flavin reductase of the present invention, thereby forming a fossil fuel and aqueous phase mixture; maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
m
lngknierie proteines
des
> Renilla luciferase and green fluorescent protein fusion genes (A Szalay et al, US)
Loma Linda University N” WO 98/14605, PCT. A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the (( humanized )) Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the ccRenillaGFP fusion protein )), which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFI? The Renillu-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.
Biocapteurs > Use of biosensors to diagnose plant diseases (L Etienne et al, FR, CH, DE)
Novartis AG and the inventors N” WO 98/21571, PCT. z Nanoparticles (TG Ewart and CA)
biosensor GT Bogle,
NoAB Immunoassay Inc and the inventors N” WO 98121587, PCT.
Analyse du genome > Method for detecting point mutations in DNA utilizing fluorescence energy transfer (LI Parkhurst et a/, US)
University of Nebraska at Lincoln N” WO 98/i 8965, PCT. A method for detecting point mutations in DNA using a fluorescently labeled oligomerit probe and fluorescence resonance energy transfer (FRET) is disclosed. The selected probe is initially labeled at each end with a fluorescence dye, which act together as a donor/acceptor pair for FRET. The fluorescence emission from the dyes changes dramatically from the duplex stage, wherein the probe is hybridized to the complementary strand of DNA, to the single strand stage, when the probe is melted to become detached from the DNA. (...)
MBthodes d’amplification z Microfabricated thermal nucleic
acid
isoam-
plification methods US)
devices (M Burns
and et al,
The Regent of the University of Michigan and Becton Dickinson and Co N” WO 98/22625, PCT. Disclosed are methods and compositions for isothermal amplification of nucleic acids in a microfabricated susbstrate. Methods and compositions for the analysis of isothermally amplified nucleic acids in a microfabricated substrate are disclosed as well. The microfabricated substrates and isothermal amplification and detection methods provided are envisioned for use in various diagnostic methods, particularly those connected with diseases characterized by altered gene sequences or gene expression.
Divers > Method for determining ion channel activity of a substance (PW Gage et a/, A’J)
The Australian National University and the inventors N” WO 98/l 3514, PCT. > Methods for determining sequence information in polynucleotides using mass spectrometry (LA Haff and IP Smirnov, US)
Perseptive Biosystems Inc N” WO 98/14616, PCT. The invention relates to method for determining sequence information in polynucleotides by combining the recent disparate technologies of mass spectromep and polynucleotide hybridization, amplification, extension and/or ligation techniques. (...) > Cleaning of conduits, especially of a photobioreactor ( DP Jenkins, GB)
Applied Photosynthetics Ltd and G Jenkins N” WO 98/l 5362, PCT. > Parallel polynucleotide sequencing method (WH Wong, US)
The Chinese University of Hong Kong N” WO 98/l 5644, PCT. The invention is directed to a method for sequencing multiple target polynucleotide segments in parallel, and to compositions and kits therefor. (...)
> Magnetically activated cell sorting for production of proteins (DL Seigel, US) The Trustees of the University of Pennsylvania N” WO 98/l 6541, PCT. The invention includes methods of isolating DNA and a protein encoded thereby, the protein being capable of binding to an antigen-bearing moiety. The methods comprise generating a DNA library expressing the protein in a virus vector. A magnetic label is added to cells expressing the antigen-bearing moiety and the cells are incubated with virus expressing the protein in the presence of an excess of unlabeled cells which do not express the antigen-bearing moiety to form a mixture, wherein the virus binds to the magnetically labeled cells. Cells having virus bound thereon are isolated from the mixture and DNA encoding the protein and the protein are obtained therefrom. > System to detect small molecule/peptide interac-
tion (LM Kauvar and EW Napolitano, US) Terrapin Technologies Inc N” WO 98116835, PCT. > Methods for generating and screening novel metabolic pathways (TC Peterson et a/, US) Chromaxome Corp N” WO 98/l 7811, PCT. The present invention relates to a novel drug discovery system for generating and screening molecular diversity. The system provides methods for mixing and cloning genetic materials from a plurality of species of organisms in combinatorial gene expression libraries to generate novel metabolic pathways and classes of compounds. (...I > Pro&d& de separation et de concentration de petites mol&ules g I’aide de pompes enzymatiques membranaires : tiacteur, dparateur/concentreur (B Maisterrena, FR) Universitb Claude-Bernard Lyon I et I’inventeur N” WO 98118904, PCT.
> Methods for preparing arrays of single-stranded DNA (AD Charles, GB) Zeneca Ltd and the inventor N” WO 98/l 8961, PCT.
Radiation-activated catalyst (RAG.), autocatalytic reactions, and protective groups are employed to achieve a highly sensitive, high resolution, radiation directed combinatorial synthesis of pattern arrays of diverse polymers. When irradiated, RAG produce catalysts that can react with enhancers, such as those involved in autocatalytic reactions. The autocatalytic reactions produce at least one product that removes protecting groups from synthesis intermediates. (...)
z Electrodes linked via conductive oligomers to nucleic acids (JF Kayyem et al, US) Clinical Micro Sensors and the inventors N” WO 98/20162, PCT. The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electrcln transfer moieties and eltctrodes to produce a new class of biomaterials, and to methods of making and using them.
> Genes encoding telomerase proteins (LA Harrington and MO Robinson, US) Amgen Inc and Amgen Canada Inc N” WO 98/21343, PCT.
z Chemical amplification for the synthesis of oatterned arrays (JE Beecher et al, US) Affymetrix Inc and the inventors N” WO 9:3/20967, PCT
Les brevets peuvent ltre obtenus ti l‘lnstitut national de la proprl6te mdustrlelle (Inpi), 26 rue de SaintPBtersbourg. 75800 Paris cedex 08. oti des coores oeuvent atre fournres sur simple demande. selon les tarifs en vigueur.
LYOPHILISAT b ) ) b )
RECHERCHE MISE AU POINT ESSAI PILOTE PRODUCTION INDUSTRIELLE FAGONNAGE
DU CHERCHEUR A L’INDUSTRIEL,DU TESTA LA PRODUCTION - Z.A. La Gandonne - Rue du Remoula’ire - 13300 SALON DE PROVENCE - FRANCE TfXO4.90.53.57.58 - Fax 04.90.5X62.08
BIOFUTUR
181
l
Seotembre
1998
51
Fermentation r Preparation of D-amino acids by direct fermentative means (IG Fotheringham et al, US)
Monsanto Co N” WO 98114602. PCT. The present invention relates to materials and method for production of natural and unnatural D-amino acids. In particular, the present invention relates to a fermentation method for the production of D-amino acids using recombinant host cells. (...)
Biopesticides > Biological insect control agents expressing insectspecific mite toxin genes, methods and compositions (L Miller et a/, US)
University of Georgia Research Foundation Inc and American Cyanamid Co N” WO 98114578, PCT. Provided herein are genetically engineered baculoviruses which express insectspecific toxins, preferably paralytic neurotoxins, under the regulatory control of strong promoters expressed early after infection and in a wide variety of insect cell. (...I
> Process for biological removal of sulphide (AJ Janssen and CJ Buisman, NL)
Thiopaq Sulfur Systems BV N” 845288, EP. The invention provides a process for the removal of sulphides from a gas stream by scrubbing the gas with an aqueous washing liquid and treating the washing liquid with sulphide-oxidising bacteria in the presence of an electron acceptor and reusing the treated liquid as a washing liquid, wherein the scrubbing and the bacterial treatment are carried out in the same reactor and nitrate is used as the electron acceptor. The process is especially useful for desulphurising natural gas at high pressure. 50 BIOFUTUR181 l Septembre1998
> Microbiological method for eliminating halogenated ethenes (P Koziollek et al, DE)
Fraunhofer-Gesellschaft zur FGrderung der Angewandten Forschung EV and the inventors N” WO 98/l 5338, PCT. The invention relates to microbiological elimination of halogenated ethenes. Said method is characterized by the fact that bacteria strains which degrade halogenated ethene and grow with ethene as a source of carbon and energy are preadapted with ethene as a source of carbon and energy for reformation and for energy supply and broughi into contact with the halogenated ethenes to be eliminated in an aerobic bioreactor. > A Rhodococcus reductase and its desulfurization of fuel (KA Gray et al,
flavin use in fossil US)
Energy Biosystems Corp N” WO 98/l 7787, PCT. The invention provides a novel flavin reductase, which has been isolated from Rhodococcus strain sp. IGTS8 and purified to homogeneity. The flavin reductase is of use in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds. The method comprises the steps of: contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of the flavin reductase of the present invention, thereby forming a fossil fuel and aqueous phase mixture; maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
m
lngknierie proteines
des
> Renilla luciferase and green fluorescent protein fusion genes (A Szalay et al, US)
Loma Linda University N” WO 98/14605, PCT. A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the (( humanized )) Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the ccRenillaGFP fusion protein )), which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFI? The Renillu-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.
Biocapteurs > Use of biosensors to diagnose plant diseases (L Etienne et al, FR, CH, DE)
Novartis AG and the inventors N” WO 98/21571, PCT. z Nanoparticles (TG Ewart and CA)
biosensor GT Bogle,
NoAB Immunoassay Inc and the inventors N” WO 98121587, PCT.
Analyse du genome > Method for detecting point mutations in DNA utilizing fluorescence energy transfer (LI Parkhurst et a/, US)
University of Nebraska at Lincoln N” WO 98/i 8965, PCT. A method for detecting point mutations in DNA using a fluorescently labeled oligomerit probe and fluorescence resonance energy transfer (FRET) is disclosed. The selected probe is initially labeled at each end with a fluorescence dye, which act together as a donor/acceptor pair for FRET. The fluorescence emission from the dyes changes dramatically from the duplex stage, wherein the probe is hybridized to the complementary strand of DNA, to the single strand stage, when the probe is melted to become detached from the DNA. (...)
MBthodes d’amplification z Microfabricated thermal nucleic
acid
isoam-
plification methods US)
devices (M Burns
and et al,
The Regent of the University of Michigan and Becton Dickinson and Co N” WO 98/22625, PCT. Disclosed are methods and compositions for isothermal amplification of nucleic acids in a microfabricated susbstrate. Methods and compositions for the analysis of isothermally amplified nucleic acids in a microfabricated substrate are disclosed as well. The microfabricated substrates and isothermal amplification and detection methods provided are envisioned for use in various diagnostic methods, particularly those connected with diseases characterized by altered gene sequences or gene expression.
Divers > Method for determining ion channel activity of a substance (PW Gage et a/, A’J)
The Australian National University and the inventors N” WO 98/l 3514, PCT. > Methods for determining sequence information in polynucleotides using mass spectrometry (LA Haff and IP Smirnov, US)
Perseptive Biosystems Inc N” WO 98/14616, PCT. The invention relates to method for determining sequence information in polynucleotides by combining the recent disparate technologies of mass spectromep and polynucleotide hybridization, amplification, extension and/or ligation techniques. (...) > Cleaning of conduits, especially of a photobioreactor ( DP Jenkins, GB)
Applied Photosynthetics Ltd and G Jenkins N” WO 98/l 5362, PCT. > Parallel polynucleotide sequencing method (WH Wong, US)
The Chinese University of Hong Kong N” WO 98/l 5644, PCT. The invention is directed to a method for sequencing multiple target polynucleotide segments in parallel, and to compositions and kits therefor. (...)
> Magnetically activated cell sorting for production of proteins (DL Seigel, US) The Trustees of the University of Pennsylvania N” WO 98/l 6541, PCT. The invention includes methods of isolating DNA and a protein encoded thereby, the protein being capable of binding to an antigen-bearing moiety. The methods comprise generating a DNA library expressing the protein in a virus vector. A magnetic label is added to cells expressing the antigen-bearing moiety and the cells are incubated with virus expressing the protein in the presence of an excess of unlabeled cells which do not express the antigen-bearing moiety to form a mixture, wherein the virus binds to the magnetically labeled cells. Cells having virus bound thereon are isolated from the mixture and DNA encoding the protein and the protein are obtained therefrom. > System to detect small molecule/peptide interac-
tion (LM Kauvar and EW Napolitano, US) Terrapin Technologies Inc N” WO 98116835, PCT. > Methods for generating and screening novel metabolic pathways (TC Peterson et a/, US) Chromaxome Corp N” WO 98/l 7811, PCT. The present invention relates to a novel drug discovery system for generating and screening molecular diversity. The system provides methods for mixing and cloning genetic materials from a plurality of species of organisms in combinatorial gene expression libraries to generate novel metabolic pathways and classes of compounds. (...I > Pro&d& de separation et de concentration de petites mol&ules g I’aide de pompes enzymatiques membranaires : tiacteur, dparateur/concentreur (B Maisterrena, FR) Universitb Claude-Bernard Lyon I et I’inventeur N” WO 98118904, PCT.
> Methods for preparing arrays of single-stranded DNA (AD Charles, GB) Zeneca Ltd and the inventor N” WO 98/l 8961, PCT.
Radiation-activated catalyst (RAG.), autocatalytic reactions, and protective groups are employed to achieve a highly sensitive, high resolution, radiation directed combinatorial synthesis of pattern arrays of diverse polymers. When irradiated, RAG produce catalysts that can react with enhancers, such as those involved in autocatalytic reactions. The autocatalytic reactions produce at least one product that removes protecting groups from synthesis intermediates. (...)
z Electrodes linked via conductive oligomers to nucleic acids (JF Kayyem et al, US) Clinical Micro Sensors and the inventors N” WO 98/20162, PCT. The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electrcln transfer moieties and eltctrodes to produce a new class of biomaterials, and to methods of making and using them.
> Genes encoding telomerase proteins (LA Harrington and MO Robinson, US) Amgen Inc and Amgen Canada Inc N” WO 98/21343, PCT.
z Chemical amplification for the synthesis of oatterned arrays (JE Beecher et al, US) Affymetrix Inc and the inventors N” WO 9:3/20967, PCT
Les brevets peuvent ltre obtenus ti l‘lnstitut national de la proprl6te mdustrlelle (Inpi), 26 rue de SaintPBtersbourg. 75800 Paris cedex 08. oti des coores oeuvent atre fournres sur simple demande. selon les tarifs en vigueur.
LYOPHILISAT b ) ) b )
RECHERCHE MISE AU POINT ESSAI PILOTE PRODUCTION INDUSTRIELLE FAGONNAGE
DU CHERCHEUR A L’INDUSTRIEL,DU TESTA LA PRODUCTION - Z.A. La Gandonne - Rue du Remoula’ire - 13300 SALON DE PROVENCE - FRANCE TfXO4.90.53.57.58 - Fax 04.90.5X62.08
BIOFUTUR
181
l
Seotembre
1998
51