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Parasites in prehistoric populations of central Europe: review and recent results
Oral Sassions I Parasitology Interwrriona147 (Suppl.) (1998) 133-281
174
O-0232
EPIDEMIOLOGY OF INFECTIONWITH TOXOPLASMA GONDII AT FERTILE WOMEN ARSIC V., MITROVIC S., KRANJ&&ZEC JOVANOVI V., MILOBRAT’OVI6
O_O234
PROSPECTIVE GONDII JINAN
I., DZAMIC A.,
AREA,
Zhongtang
The protozoan Toxoplasma gondii is usually harmless, opportunistic parasite in humans but beside that it continues to cause significant morbidity and mo+Iity. While generally most postnatal infection of healthy immunocompetam persons da not cause any clinical manifestations. in small proportion
symptoms as fever, lethargi and lymphadenitis may occur. However, an infection leads to the formation of cysts and thus to immunity. Coleffed sera from 2835 woman in reproductive period ticb were tested oh anti-Toxoplasma gondii antibodis between 1994. and 1997. were examined. IgG and IgM antibodies we determined by ELISA (Toxonosika IgGIi and Toxonostika IghlrI Organon Tehnika, Boxtel, NL). Antitoxoplasma antibodys (IgG and/or IgM) were detected in 1335 persons (47,IK). Only IgG antibodies were detected in 1213 persons (42,8%) and the part of Igh4 only antibodis were detected in 52 cases (l,82%). Both IgG and IgM were detected in 99 cases (3.5%). In the next step srmm with positive IgM antibodies were examined for activ toxoplasmosisinfection. Our expectationscorrespond with this epidemiologicalstudie‘s results and once again it show that congenital toxoplasmosis can be markedlly reduced by serologic srweillance of fertile and pregnant women.
Zhao.
Department Jinan
OF TOXOPLASMA WOMEN
IN
CHINA
FR Hao,
of
260012,PR
To understand of pregnant making
of health
randomly
to the of
(118).
reapectlvely.
cohort
Their
endomstrlum, and
dead
mrological
fatua
during
labour,still were 7.01, firat
birth
or after
birth
signSficantly
the
rate
of
than
a
that
and lFA:(l)
and the
no evideaos or
etc) for
the
infection
up
lochia.
placenta.
astiological
were carefully
and analywd. defects of
of the third
group
f&t
OR
the
group.
rea,,ectivsly.
Differencea not
were
growth the
rate
active
w*re folloaed
fluid,
rata
The
between The
was third
of %7.4%. gondii
of congenital
were
valuea
significant.
retardation
lnfectin.
Toxoplauna
incidence
prwnant
apontw,eo”abortion,premature
transmiwion
congenital
for
according
(a) r-t
group
with
data
Bol¶
into 8 groupa
o”tcopI~
intrauterine
congenital of
not group
The
total
infection
wa
Tonqdasmosln
at
was 0.177,.
Recentor women
problem8
of the
this problem
is
active one
population.
Toroplarma
of
the
OF CENTRAL RESULTS
gondii
infection
important public
An intervention
haa bean formulated
O-0235 PARASITES
encountered aczcrding to the sex and intensity ofthe contamination..
of
second
correlated
Conc1u&m:
tatistkal meaningful differences were not
wa# employed.
congenital
delivery
rate.
gondli
the
thir area.
amniotic
of
8.76 apd 9.34
and
pregnant
place from which they were collected. Oocysts were shown in 4.6% ofthe cays and 34.6% of them were positive after dyetest(>1/16).withre0arddtheapeandtheenviromentfromwhichthey
provide
IgM ELJSA
and congenital
higher
8.44,
Univsr~ity,
of Toxopkumr
to
or newborns,
diagnods
The incidence
0.87$6,
sucrose and the serokgii dye test Our main object ~85 to detect the cc-cystmicroscopicaily and by detectiw antibodk against T. gondii , using sabin f&man dye test. Our findings on ths test cats wers anatyxed according to the sex , ageand M
study
( blood,
RaavILl:
frequency
In this study 107 cets were sekted randomly from North west of Iran. Stool specimsnts of the test animals were tested by direct examination , wing
in
Pregnancy
collected
showed
Tabriz University of Medical Scincer.
that
were divided
by IgG,
Samplea
significantly
CONTAMNATION OF CATS WITHTOXOPLASMA GONDll IN NORTH WEST OF IRAN (1s - 96).
policy
4ectc.d.
aerodiagnosls
incidence
O-0233
m
Infection(nsz): (0)put infection(147);
infectlen
Jia.
Msdlorl
the barmfulnau
aome.n,
Matbod#:Prospective aome.n.
and CX
Shandong
China
Pupore: decision
YZ Pan,
Epidemiology,
infection
the
wllec3ed(hospital and nonhospital c&ction).S diierences were found (pcO.05) , butsign~nt
STUDY
OF PREGNANT
D.
Instituteof Microbiology and Immunology, School of Medicine, University of Belgrade, Belgrade, Yugoslavia
Kazemi A Jamsty R. Tabrk Iran Dept d Pare&logy. Keywords:Tor&asmagondii,Cat.
COHORT
INFECTION
Program
of
health against
in the area.
IN PREHISTORIC POPULATIONS EUROPE: REVIEW AND RECENT
As&k H, Auer H, Picher 0 Department of Medical Parasitology, Hygiene, University of Vienna, Austria
Clinical
Institute
of
During the past years we had several opportunities to examine various human remains of prehistoric periods of Central Europe for parasites, the most famous of them being the spectacular wellpreserved Neolithic glacier mummy (at least 5200 years old), found in the Tyrolese Alps in an al&de of 3200 m. The body was investigated for ectoparasites, and samples from the jejunum as well as from the colon were examined for intestinal parasites. Moreover, we have examined and have continuously been studying large numbers of samples of prehistoric human faeces excellently preserved in salt-mineral which were left in the mines by prehistoric Alpine salt miners in the first millenium BC. After rehydration with 0.3% trisodium phosphate and/or fixation of the samples in SAF various diagnostic procedures were applied including staining, microscopy and teSts for coproantigens. The colon of the Neolithic mummy proved to contain large numbers of eggs of Ttichunk rrichium; they represent by far the oldest evidence of whipworm in a human corpse. In the excrements of the salt miners eggs of Ascuti fumbticoides, Trichurk rrichium, Taenia, and, most probably, Dicmcoelium dendriricum , were found. These findings allow several conclusions on the epidemiology of parasitic diseases in Central Europe 2000 to 5000 years ago.
174
O-0232
EPIDEMIOLOGY OF INFECTIONWITH TOXOPLASMA GONDII AT FERTILE WOMEN ARSIC V., MITROVIC S., KRANJ&&ZEC JOVANOVI V., MILOBRAT’OVI6
O_O234
PROSPECTIVE GONDII JINAN
I., DZAMIC A.,
AREA,
Zhongtang
The protozoan Toxoplasma gondii is usually harmless, opportunistic parasite in humans but beside that it continues to cause significant morbidity and mo+Iity. While generally most postnatal infection of healthy immunocompetam persons da not cause any clinical manifestations. in small proportion
symptoms as fever, lethargi and lymphadenitis may occur. However, an infection leads to the formation of cysts and thus to immunity. Coleffed sera from 2835 woman in reproductive period ticb were tested oh anti-Toxoplasma gondii antibodis between 1994. and 1997. were examined. IgG and IgM antibodies we determined by ELISA (Toxonosika IgGIi and Toxonostika IghlrI Organon Tehnika, Boxtel, NL). Antitoxoplasma antibodys (IgG and/or IgM) were detected in 1335 persons (47,IK). Only IgG antibodies were detected in 1213 persons (42,8%) and the part of Igh4 only antibodis were detected in 52 cases (l,82%). Both IgG and IgM were detected in 99 cases (3.5%). In the next step srmm with positive IgM antibodies were examined for activ toxoplasmosisinfection. Our expectationscorrespond with this epidemiologicalstudie‘s results and once again it show that congenital toxoplasmosis can be markedlly reduced by serologic srweillance of fertile and pregnant women.
Zhao.
Department Jinan
OF TOXOPLASMA WOMEN
IN
CHINA
FR Hao,
of
260012,PR
To understand of pregnant making
of health
randomly
to the of
(118).
reapectlvely.
cohort
Their
endomstrlum, and
dead
mrological
fatua
during
labour,still were 7.01, firat
birth
or after
birth
signSficantly
the
rate
of
than
a
that
and lFA:(l)
and the
no evideaos or
etc) for
the
infection
up
lochia.
placenta.
astiological
were carefully
and analywd. defects of
of the third
group
f&t
OR
the
group.
rea,,ectivsly.
Differencea not
were
growth the
rate
active
w*re folloaed
fluid,
rata
The
between The
was third
of %7.4%. gondii
of congenital
were
valuea
significant.
retardation
lnfectin.
Toxoplauna
incidence
prwnant
apontw,eo”abortion,premature
transmiwion
congenital
for
according
(a) r-t
group
with
data
Bol¶
into 8 groupa
o”tcopI~
intrauterine
congenital of
not group
The
total
infection
wa
Tonqdasmosln
at
was 0.177,.
Recentor women
problem8
of the
this problem
is
active one
population.
Toroplarma
of
the
OF CENTRAL RESULTS
gondii
infection
important public
An intervention
haa bean formulated
O-0235 PARASITES
encountered aczcrding to the sex and intensity ofthe contamination..
of
second
correlated
Conc1u&m:
tatistkal meaningful differences were not
wa# employed.
congenital
delivery
rate.
gondli
the
thir area.
amniotic
of
8.76 apd 9.34
and
pregnant
place from which they were collected. Oocysts were shown in 4.6% ofthe cays and 34.6% of them were positive after dyetest(>1/16).withre0arddtheapeandtheenviromentfromwhichthey
provide
IgM ELJSA
and congenital
higher
8.44,
Univsr~ity,
of Toxopkumr
to
or newborns,
diagnods
The incidence
0.87$6,
sucrose and the serokgii dye test Our main object ~85 to detect the cc-cystmicroscopicaily and by detectiw antibodk against T. gondii , using sabin f&man dye test. Our findings on ths test cats wers anatyxed according to the sex , ageand M
study
( blood,
RaavILl:
frequency
In this study 107 cets were sekted randomly from North west of Iran. Stool specimsnts of the test animals were tested by direct examination , wing
in
Pregnancy
collected
showed
Tabriz University of Medical Scincer.
that
were divided
by IgG,
Samplea
significantly
CONTAMNATION OF CATS WITHTOXOPLASMA GONDll IN NORTH WEST OF IRAN (1s - 96).
policy
4ectc.d.
aerodiagnosls
incidence
O-0233
m
Infection(nsz): (0)put infection(147);
infectlen
Jia.
Msdlorl
the barmfulnau
aome.n,
Matbod#:Prospective aome.n.
and CX
Shandong
China
Pupore: decision
YZ Pan,
Epidemiology,
infection
the
wllec3ed(hospital and nonhospital c&ction).S diierences were found (pcO.05) , butsign~nt
STUDY
OF PREGNANT
D.
Instituteof Microbiology and Immunology, School of Medicine, University of Belgrade, Belgrade, Yugoslavia
Kazemi A Jamsty R. Tabrk Iran Dept d Pare&logy. Keywords:Tor&asmagondii,Cat.
COHORT
INFECTION
Program
of
health against
in the area.
IN PREHISTORIC POPULATIONS EUROPE: REVIEW AND RECENT
As&k H, Auer H, Picher 0 Department of Medical Parasitology, Hygiene, University of Vienna, Austria
Clinical
Institute
of
During the past years we had several opportunities to examine various human remains of prehistoric periods of Central Europe for parasites, the most famous of them being the spectacular wellpreserved Neolithic glacier mummy (at least 5200 years old), found in the Tyrolese Alps in an al&de of 3200 m. The body was investigated for ectoparasites, and samples from the jejunum as well as from the colon were examined for intestinal parasites. Moreover, we have examined and have continuously been studying large numbers of samples of prehistoric human faeces excellently preserved in salt-mineral which were left in the mines by prehistoric Alpine salt miners in the first millenium BC. After rehydration with 0.3% trisodium phosphate and/or fixation of the samples in SAF various diagnostic procedures were applied including staining, microscopy and teSts for coproantigens. The colon of the Neolithic mummy proved to contain large numbers of eggs of Ttichunk rrichium; they represent by far the oldest evidence of whipworm in a human corpse. In the excrements of the salt miners eggs of Ascuti fumbticoides, Trichurk rrichium, Taenia, and, most probably, Dicmcoelium dendriricum , were found. These findings allow several conclusions on the epidemiology of parasitic diseases in Central Europe 2000 to 5000 years ago.