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Pathogen load and expression of immune-related genes in mesenteric lymph-nodes of pigs experimentally infected with Salmonella typhimurium
New Biotechnology · Volume 27S · April 2010
RUNX2 might be involved in the establishment of OC lesion in pigs. doi:10.1016/j.nbt.2010.01.076
[P1.70] Pathogen load and expression of immune-related genes in mesenteric lymph-nodes of pigs experimentally infected with Salmonella typhimurium R.P. Martins ∗ , M. Collado-Romero, C. Arce, J.J. Garrido University of Córdoba, Spain
Salmonellosis in swine has recently attracted the attention of research efforts due to its potential as a zoonotic agent. Salmonella typhimurium is often present in mesenteric lymph-nodes (MLN) and a relevant transcriptional response in these organs has been reported during the infection of pigs by this pathogen. So, this work aimed to analyze the pathogen load and changes in the expression of immune-related genes in mesenteric lymph nodes of pigs experimentally infected with S. typhimurium.
POSTERS ABSTRACTS 1
Sixteen piglets free of Salmonella were used in this study. Four control pigs were necropsied two hours before the experimental infection and the remaining animals were orally challenged with 108 cfu of S. typhimurium. Afterwards, four infected pigs were necropsied at 1 day post infection (dpi), 2 dpi and 6 dpi. Samples of MLN from all animals were aseptically collected and immediately frozen in liquid nitrogen for DNA and RNA isolation. Transcriptional changes were evaluated by qPCR. The expression of mRNA of 34 immune-related genes was quantified and the resulting data were analyzed by ANOVA and Duncan post-hoc test. Finally, the access of the S. typhimurium load in MLN was evaluated by TaqMan real-time PCR assays. Fourteen immune-related genes showed significant changes in expression in infected animals as compared to controls (Figure 1). Two of them (CCL28 and TLR8) just showed significant differences among the three infected groups. The quantification of Salmonella revealed a higher load at 2 dpi when compared to 1 dpi and 6 dpi. The infection by S. typhimurium resulted in an up regulation of genes related to the innate/inflammatory response mainly at 2 dpi and the decrease in the pathogen load at 6 dpi could be attributed to this transcriptional response. Furthermore, the decrease in expression of some genes could consist in a mechanism employed by the bacteria to maintain itself in the MLN of infected animals.
Figure 1 Fold change in expression in infected pigs as compared to controls. doi:10.1016/j.nbt.2010.01.077
www.elsevier.com/locate/nbt S49
RUNX2 might be involved in the establishment of OC lesion in pigs. doi:10.1016/j.nbt.2010.01.076
[P1.70] Pathogen load and expression of immune-related genes in mesenteric lymph-nodes of pigs experimentally infected with Salmonella typhimurium R.P. Martins ∗ , M. Collado-Romero, C. Arce, J.J. Garrido University of Córdoba, Spain
Salmonellosis in swine has recently attracted the attention of research efforts due to its potential as a zoonotic agent. Salmonella typhimurium is often present in mesenteric lymph-nodes (MLN) and a relevant transcriptional response in these organs has been reported during the infection of pigs by this pathogen. So, this work aimed to analyze the pathogen load and changes in the expression of immune-related genes in mesenteric lymph nodes of pigs experimentally infected with S. typhimurium.
POSTERS ABSTRACTS 1
Sixteen piglets free of Salmonella were used in this study. Four control pigs were necropsied two hours before the experimental infection and the remaining animals were orally challenged with 108 cfu of S. typhimurium. Afterwards, four infected pigs were necropsied at 1 day post infection (dpi), 2 dpi and 6 dpi. Samples of MLN from all animals were aseptically collected and immediately frozen in liquid nitrogen for DNA and RNA isolation. Transcriptional changes were evaluated by qPCR. The expression of mRNA of 34 immune-related genes was quantified and the resulting data were analyzed by ANOVA and Duncan post-hoc test. Finally, the access of the S. typhimurium load in MLN was evaluated by TaqMan real-time PCR assays. Fourteen immune-related genes showed significant changes in expression in infected animals as compared to controls (Figure 1). Two of them (CCL28 and TLR8) just showed significant differences among the three infected groups. The quantification of Salmonella revealed a higher load at 2 dpi when compared to 1 dpi and 6 dpi. The infection by S. typhimurium resulted in an up regulation of genes related to the innate/inflammatory response mainly at 2 dpi and the decrease in the pathogen load at 6 dpi could be attributed to this transcriptional response. Furthermore, the decrease in expression of some genes could consist in a mechanism employed by the bacteria to maintain itself in the MLN of infected animals.
Figure 1 Fold change in expression in infected pigs as compared to controls. doi:10.1016/j.nbt.2010.01.077
www.elsevier.com/locate/nbt S49