Pathogenesis of Alopecia areata totalis

131 THE EFFECT OF TESTOSTERONEON FOLLICULAECELLS OF HAMSTER -AN ULTRASTRUCTURALSTUDY-

EFFECTS OF 1,25(OH)2D3 CELLS IN VITRO

ON HUMAN HAIR FOLLICLE

S.Takahashi and M.Morohashi.Departmentof Dermatology,Faculryof Medicine, Tayama Medical and PharmaceuticalUniv., Toyama

Y.Sadamoto,S.Arase,R.Kuwana,and K.Takeda. Department of Dermatology,Tokushima Medical Coll.,Tokushima.

The occulusion of pilosebaceous duct is well known as one of the pathogenesis of comedo formation.This occulusion is mainly caused by abnormal keratinizacion of infundibulum,buf its precise mechanism has not been yet elucidated. In order to clarify the effect of androgen on this abnormal keratinization, we examined the ulcrastruccural changes of keratinizing condition on infundibulum of male hamster treated with androgen one to three weeks using electron microscope. At three weeks, the appearance of lipid vacuoles in the cells from upper granular to horny layer, the coarseness of keratohyalin granules,and the decrease in number of Conofilaments and lamellar bodies in the cells at the infundibulum were seen. From the present findings the abnormal follicular kerarinizarion at 2 to 3 weeks treated with testosterone was considered to be similar to the findings of early change of experimental cornedones on rabbit ear treated with oleic acid. These findings may suggest that testosterone exerts an influence on abnormal kerarinization of follicular epiehelium ae the early stage of comedo formacian.

Th$ effects of 1,25-dihvdroxvvitamin D3WD3) on the and differentiation of cultured human follicle cells(HFC) were studied.VD3 markedly affected the growth of HFC.It caused a dose-dependent decrease in both plating efficiency and colony growth,and inhibited DNA synthesis of HFC.VD3 also caused an increase in cell size, stimulated cornified envelope formation and induced marvholosical chanses of HFC showins terminal differentia&on.HFC was c&firmed,but indir&tly,to be target cells VD3 seems to be a potent inhibitor of proliferation as weli as a potent stimulator of terminal differentiation of HFC.Our findings may give helpful clues to consider the mechanism of alopecia seen in patients with vitamin D-dependent rickets type 2.

EFFECTS OF ON CULTURED

OIHYDROTESTOSTERONE PAPILLA CELLS

AND

ITS

RECEPTORS

I

S. Nishiyama(Depart?ent K. Katsuoka. K.Inamula. Kitasato Univ.) Dermatology, K. Sakabe(Oepartment of Anatomy, Tokai Univ.) T. Ogawa(Kanebo K.K., Cosmetic Laboratory)

..~~

OR.Kuwana,

S.Arase,

ON CULTURED

Y.Sadamoto

RELATIONSHIP

BETWEEN MALE-PATTERN

AND THICKNESS

Of

Papilla cells(P.C) grown by the isolation and cultivation of human hair papilla in the anagen phase show a characteristic form and behavior. The differences of their collagen syrlthesis from that of dermal fibroblasts(0.F) have already been found and reported. This report will describe a study of the androgen receptors of P.C and the effects of androgen on P.C in vitro. Cytochemical staining of P.C using dihydrotestosterone (DHT)-peroxidase conjugate gave positive reactions in the nucleus of P.C. This clearly indicates the existence of different androgen receptors in P.C from those of O.F. similar studies of estrogen receptors showed no While, significant results between P.C and D.F. Further cultivation of P.C with the addition of DHT for two weeks showed an increased cell count and thymidine uptake compared with the control. Above results suggest that the possible effects of androgen through papilla cells in hair papilla are part of the hormonal mechanism of the hair cycle.

EFFECT OF MINOXIDIL CELLS

growth

0

S. Toshitani. Fukuoka

Department

University,

of Dermatology,

A. Nagasawa.

In 1954, R. Young concluded relation

between

of the measure

male-pattern

cases with

that the thickness of the scalp has no

baldness

of the promotion

of the scalp-tension, In the average, the average Casey

as the result

ray.

of hair regrowth

by the looseness

50 cases with

the baldness

the scalp of the cases with non-baldness

showed

the result

baldness

in the vertex.

below the average value 0.3. male-pattern

was thicker

In the sinciput

to the contrary.

were observed

with overwhelmingly

that the thickness of the. vertex minus

might foretell

and 50

by the echography.

than that of the cases with baldness Male-pattern

and non-baldness

we investigated

the non-baldness

School of Medicine,

Takeda Shinryosho

in the scalp by soft Grew

the viewpoint

From

BALDNESS

OF THE SCALP

These results bladness

with

majority

in the

that of the sinciputequal led to speculation

the significant

that we

difference.

HUMAN HAIR FOLLICLE Pathogenesls of Alopeaa areatatotalis

and K.Takeda S. Okabe.

Department of Dermatology, School of Medicine, The University of Tokushima. Tokushima, Japan. Minoxidil(MX),whether applied topically or taken orally, has been said to increase the thickness and length of hairs. Knowing the direct action of MX on scalp hairs,we investigated the effect of MX on hair follicle cells(HFC)in vitro. HFC were derived from plucked anagen hair follicles and cultured according to the method previously reported by us. Growth of HFC was not stimulated by MX at all concentrations tested of 1,lO and 100 pg/ml. MX had no effect on the differentiation of HFC. Terminal differentiation such as stratification of cells,appearance of large denucleated cells and cornified envelope formation was seen at MX level of 10 pg/ml as in control. We found no evidence that MX activates the proliferation or modulates the differentiation of HFC in vitro.

Yokote.

Department

of Dermatology.

I consider maintenance

Alopecia factors

areata play

cia areata

totahs.Thele

peripheral

circulation

totalis

from

an important

part

IS an autonomic in the

causesa stress the family cm

Hraka

General

Hospltai

Japan

They

focus

This

is a

brnd

restrictlon.

and.

excessively

stereotype.

The are his

Also

when

of attension

upon

his

family

Alopecm

effort

members

areata

to a case, leaves

him

His condition

therapy. disorder,

and

there

The Alope-

IS poor

Alopecia also

circle.

are concern

on a treatment

of Alopecia

officious

Family

As a result,

is a w&us

as well as case

family

of

m the pychosomatic imbalance.

There

on their

Family

interference.

hair.

standpoint

about

Alope-

of Alopecia.

tot&s

are

he feels

a double

annoyed

alone,

he focuses

shows

no sign

the

by

thar

spotlight

of improvement.