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Patterns of axonal collateralization of single corticocortical projection neurons in the rat presubiculum
S190
Abstracts
findings suggest that in addition to its effects on spatial cognition and LTP, PUFA supplementation also facilitates neurogenesis in SAMP. doi:10.1016/j.neures.2009.09.1023
P2-n10 Patterns of axonal collateralization of single corticocortical projection neurons in the rat presubiculum Yoshiko Honda 1 , Takahiro Furuta 2 , Takeshi Kaneko 2 , Hideshi Shibata 3 , Hiroshi Sasaki 1 1
Dept Anat, Tokyo Women’s Med Univ, Tokyo, Japan; 2 Dept Morphol Brain Sci, Kyoto Univ, Kyoto, Japan; 3 Lab Vet Anat, Tokyo Univ Agri & Tech, Tokyo, Japan We studied the axonal morphology of single presubicular neurons in the rat, with a viral vector expressing membrane-targeted GFP. A single pyramidal neuron in layer III of the presubiculum (PreS) had six axon collaterals, three of which reached layer III of the medial entorhinal area (MEA), one passed through the dorsal hippocampal commissure, and two recurrently reached layers V and VI of PreS. Another single pyramidal neuron in layer V had four axon collaterals that projected to layers V and VI of the retrosplenial cortex and one recurrent collateral that reached layer VI of PreS. A single non-pyramidal layer VI neuron had four axon collaterals; one innervated layers V and VI of MEA, one entered the white matter, and two recurrently reached layers V and VI of PreS. Our data demonstrate for the first time some characteristic patterns of axonal collateralization of single corticocortical projection neurons in each layer of the rat presubiculum. doi:10.1016/j.neures.2009.09.1024
P2-n13 The role of parietal-prefrontal interaction in episodic memory Charles R.E. Wilson, David Gaffan Dept Experimental Psychology, Oxford University, UK Activations in posterior parietal cortex (PPC) are frequently seen in functional neuroimaging studies of human episodic memory, yet the precise role of parietal cortex and its interactions with other structures remain unclear (Olson & Berryhill, 2009). We investigated the role of interaction between PPC and prefrontal cortex (PFC) in episodic memory using the crossed unilateral disconnection technique successful in studying PFC-IT interaction (Wilson et al., 2008; Browning et al., 2008). Three rhesus monkeys learned the object-in-place scenes task, a test of episodic memory (Gaffan, 1994). The monkeys received a crossed unilateral disconnection of PPC from PFC in two stages, all receiving the unilateral PPC ablation first. Monkeys with only unilateral PPC ablation were unimpaired at the task, and demonstrated no behavioural disorders, such as visuospatial neglect, often associated with such lesions. Monkeys with the completed disconnection demonstrated a mild impairment in the scenes task, although this impairment was much less extensive than that following disconnection of IT from PFC (Browning et al., 2005). doi:10.1016/j.neures.2009.09.1027
P2-n14 Behavioral assessment of spatial and non-spatial relational memory in Capuchin monkeys (Cebus libidinosus) Maria Clotilde Henriques Tavares 1 , Antonella Gasbarri 2 , Larissa Carneiro 1 , Carolina Arruda 1 , Lorena Silva 1 , Isabel Waga 1 , Lais Coelho 1 , Carlos Tomaz 1 1
P2-n11 Behavioral roles of the striatonigral neural pathway in reinforcement learning Ryoji Fukabori 1 , Nobuyuki Kai 1 , Kenta Kobayashi 1 , Kana Okada 1 , Yasunobu Yasoshima 2 , Yuji Tsutsui 3 , Kazuto Kobayashi 1 1 Dept Mol Genet, Fukushima Medical University School of Medicine, Fukushima, Japan; 2 Dept Behav Physiol, Osaka University, Osaka, Japan; 3 Dept Human Support System, Fukushima University, Fukushima, Japan
We assayed the behavioral performance of the mutant mice lacking the striatonigral neurons by using an operant conditioning task with visual discrimination, because striatal dopamine-deprivation is known to impair this behavior. The mutant mice were generated that express the human interleukin-2 receptor alpha-subunit, a target for the recombinant immunotoxin, under the control of dopamine D1 receptor (D1R) gene. The immunotoxin injection eliminated the majority of the striatonigral neurons containing D1R in the mutants with no influence on other striatal neurons. In the operant conditioning task, the correct response time was delayed in the immunotoxin-injected mutant mice, whereas the correct response ratio was not changed in these mice. These results suggest that the striatonigral neurons play a role in response timing but have no effect on selection of true-false response. doi:10.1016/j.neures.2009.09.1025
Fukushima University, Japan We examined the relationship between rats’ auditory memory and central cholinergic system using the DCD task, which is a new paradigm of measuring the short-term memory of rats (Tsutsui et al., 2007). In this task, a tone stimulus of either 2 KHz(L) or 8 KHz(H) was presented to rats for 3 seconds. After either retention interval of 0, 1, 3, 5, or 7 seconds, rats were required to press the right lever in response to H-tone and the left lever in response to L-tone to gain a food pellet reward. After the training of the DCD task, rats received an IP injection of anticholinergic drugs. Although scopolamine(SCOP) at 0.1 mg/kg did not affect their performance, SCOP at 0.2 mg/kg decreased the correct response compared to the saline condition. Moreover, a unilateral radiofrequency lesion of the nucleus basalis magnocellularis (NBM) decreased the percentage of correct responses, whereas the sham lesion did not. These results suggest that rats’ auditory memory is affected by central cholinergic neurons, in particular, a pathway from the NBM to the cortex have an important role in the auditory memory system. doi:10.1016/j.neures.2009.09.1026
2
University of L’Aquila, Italy
Spatial and non-spatial information representations of the environment is essential to primate survival. This study tested both types of memory in 8 adult captive capuchin monkeys. Two different kinds of food reward were given to them before each trial as condition cue to signal the location of food rewards hidden inside boxes presented as an inner or outer square. Animals were tested when the baited array was constant or varied randomly between trials. Monkeys were able to use spatial memory to predict the location of food on both arrays and learned the inner square faster than the outer one, suggesting that they seem to organize their search trajectory and minimize the memory demand of the task when resources are clustered. Capuchins were also able to use the food rewards given as a cue to predict the baited array. These findings indicate that they can integrate where and what information and utilize relational information beyond the spatial domain. doi:10.1016/j.neures.2009.09.1028
P2-n15 Single neurons in monkey dorsal raphe nucleus responded in multi-trial reward schedule task with different reward amount Kiyonori Inaba 1 , Takashi Mizuhiki 1 , Koji Toda 1 , Shigeru Ozaki 1 , Kanako Yaguchi 1 , Munetaka Shidara 1,2 1 2
P2-n12 Rats’ auditory memory and central cholinergic system: effects of anticholinergic drugs and unilateral lesion to the nucleus basalis magnocellularis Yuji Tsutsui
University of Brasilia, Brasilia, Brazil;
Grad. Sch. of Comprehensive Human Sci., Univ of Tsukuba, Japan; Neurosci. Res. Inst., AIST, Ibaraki, Japan
Recent studies revealed that single neurons in dorsal raphe nucleus (DR) carry information on reward. We reported that the neurons in/around DR of rhesus monkey showed responses that were reward contingent during multi-trial reward schedules (JNNS meeting in Tokyo, 2008). We now ask whether they encode signals related to reward amount. We manipulated both schedule length (1, 2 and 3 trials) and reward amount (1, 2 and 3 drops of liquid) in modified version of reward schedules. During the task the length and brightness of visual cue indicated the schedule progress and reward amount, respectively. The error rate was modulated both by schedule progress and reward amount. We recorded 12 neurons from DR. The activity of 6/12 neurons were significantly different in relation to reward amount, suggesting that DR might have information related to reward amount. doi:10.1016/j.neures.2009.09.1029
P2-n16 Effects of reward proximity and amount on neural activity in the rostral anterior cingulate cortex Koji Toda 1,2 , Takashi Mizuhiki 1 , Yasuko Sugase-Miyamoto 3 , Kiyonori Inaba 1 , Shigeru Ozaki 1 , Barry J. Richmond 4 , Munetaka Shidara 1 1
Grad Sch Comprehensive Human Science, Univ Tsukuba, Japan; Japan; 3 Neurosci Res Inst, AIST, Japan; 4 NIMH, NIH, USA
2
JSPS,
Anterior cingulate cortex (ACC) is thought to be related to reward expectancy. We ask how sub-regions of the ACC differ in their reward representation. To study two
Abstracts
findings suggest that in addition to its effects on spatial cognition and LTP, PUFA supplementation also facilitates neurogenesis in SAMP. doi:10.1016/j.neures.2009.09.1023
P2-n10 Patterns of axonal collateralization of single corticocortical projection neurons in the rat presubiculum Yoshiko Honda 1 , Takahiro Furuta 2 , Takeshi Kaneko 2 , Hideshi Shibata 3 , Hiroshi Sasaki 1 1
Dept Anat, Tokyo Women’s Med Univ, Tokyo, Japan; 2 Dept Morphol Brain Sci, Kyoto Univ, Kyoto, Japan; 3 Lab Vet Anat, Tokyo Univ Agri & Tech, Tokyo, Japan We studied the axonal morphology of single presubicular neurons in the rat, with a viral vector expressing membrane-targeted GFP. A single pyramidal neuron in layer III of the presubiculum (PreS) had six axon collaterals, three of which reached layer III of the medial entorhinal area (MEA), one passed through the dorsal hippocampal commissure, and two recurrently reached layers V and VI of PreS. Another single pyramidal neuron in layer V had four axon collaterals that projected to layers V and VI of the retrosplenial cortex and one recurrent collateral that reached layer VI of PreS. A single non-pyramidal layer VI neuron had four axon collaterals; one innervated layers V and VI of MEA, one entered the white matter, and two recurrently reached layers V and VI of PreS. Our data demonstrate for the first time some characteristic patterns of axonal collateralization of single corticocortical projection neurons in each layer of the rat presubiculum. doi:10.1016/j.neures.2009.09.1024
P2-n13 The role of parietal-prefrontal interaction in episodic memory Charles R.E. Wilson, David Gaffan Dept Experimental Psychology, Oxford University, UK Activations in posterior parietal cortex (PPC) are frequently seen in functional neuroimaging studies of human episodic memory, yet the precise role of parietal cortex and its interactions with other structures remain unclear (Olson & Berryhill, 2009). We investigated the role of interaction between PPC and prefrontal cortex (PFC) in episodic memory using the crossed unilateral disconnection technique successful in studying PFC-IT interaction (Wilson et al., 2008; Browning et al., 2008). Three rhesus monkeys learned the object-in-place scenes task, a test of episodic memory (Gaffan, 1994). The monkeys received a crossed unilateral disconnection of PPC from PFC in two stages, all receiving the unilateral PPC ablation first. Monkeys with only unilateral PPC ablation were unimpaired at the task, and demonstrated no behavioural disorders, such as visuospatial neglect, often associated with such lesions. Monkeys with the completed disconnection demonstrated a mild impairment in the scenes task, although this impairment was much less extensive than that following disconnection of IT from PFC (Browning et al., 2005). doi:10.1016/j.neures.2009.09.1027
P2-n14 Behavioral assessment of spatial and non-spatial relational memory in Capuchin monkeys (Cebus libidinosus) Maria Clotilde Henriques Tavares 1 , Antonella Gasbarri 2 , Larissa Carneiro 1 , Carolina Arruda 1 , Lorena Silva 1 , Isabel Waga 1 , Lais Coelho 1 , Carlos Tomaz 1 1
P2-n11 Behavioral roles of the striatonigral neural pathway in reinforcement learning Ryoji Fukabori 1 , Nobuyuki Kai 1 , Kenta Kobayashi 1 , Kana Okada 1 , Yasunobu Yasoshima 2 , Yuji Tsutsui 3 , Kazuto Kobayashi 1 1 Dept Mol Genet, Fukushima Medical University School of Medicine, Fukushima, Japan; 2 Dept Behav Physiol, Osaka University, Osaka, Japan; 3 Dept Human Support System, Fukushima University, Fukushima, Japan
We assayed the behavioral performance of the mutant mice lacking the striatonigral neurons by using an operant conditioning task with visual discrimination, because striatal dopamine-deprivation is known to impair this behavior. The mutant mice were generated that express the human interleukin-2 receptor alpha-subunit, a target for the recombinant immunotoxin, under the control of dopamine D1 receptor (D1R) gene. The immunotoxin injection eliminated the majority of the striatonigral neurons containing D1R in the mutants with no influence on other striatal neurons. In the operant conditioning task, the correct response time was delayed in the immunotoxin-injected mutant mice, whereas the correct response ratio was not changed in these mice. These results suggest that the striatonigral neurons play a role in response timing but have no effect on selection of true-false response. doi:10.1016/j.neures.2009.09.1025
Fukushima University, Japan We examined the relationship between rats’ auditory memory and central cholinergic system using the DCD task, which is a new paradigm of measuring the short-term memory of rats (Tsutsui et al., 2007). In this task, a tone stimulus of either 2 KHz(L) or 8 KHz(H) was presented to rats for 3 seconds. After either retention interval of 0, 1, 3, 5, or 7 seconds, rats were required to press the right lever in response to H-tone and the left lever in response to L-tone to gain a food pellet reward. After the training of the DCD task, rats received an IP injection of anticholinergic drugs. Although scopolamine(SCOP) at 0.1 mg/kg did not affect their performance, SCOP at 0.2 mg/kg decreased the correct response compared to the saline condition. Moreover, a unilateral radiofrequency lesion of the nucleus basalis magnocellularis (NBM) decreased the percentage of correct responses, whereas the sham lesion did not. These results suggest that rats’ auditory memory is affected by central cholinergic neurons, in particular, a pathway from the NBM to the cortex have an important role in the auditory memory system. doi:10.1016/j.neures.2009.09.1026
2
University of L’Aquila, Italy
Spatial and non-spatial information representations of the environment is essential to primate survival. This study tested both types of memory in 8 adult captive capuchin monkeys. Two different kinds of food reward were given to them before each trial as condition cue to signal the location of food rewards hidden inside boxes presented as an inner or outer square. Animals were tested when the baited array was constant or varied randomly between trials. Monkeys were able to use spatial memory to predict the location of food on both arrays and learned the inner square faster than the outer one, suggesting that they seem to organize their search trajectory and minimize the memory demand of the task when resources are clustered. Capuchins were also able to use the food rewards given as a cue to predict the baited array. These findings indicate that they can integrate where and what information and utilize relational information beyond the spatial domain. doi:10.1016/j.neures.2009.09.1028
P2-n15 Single neurons in monkey dorsal raphe nucleus responded in multi-trial reward schedule task with different reward amount Kiyonori Inaba 1 , Takashi Mizuhiki 1 , Koji Toda 1 , Shigeru Ozaki 1 , Kanako Yaguchi 1 , Munetaka Shidara 1,2 1 2
P2-n12 Rats’ auditory memory and central cholinergic system: effects of anticholinergic drugs and unilateral lesion to the nucleus basalis magnocellularis Yuji Tsutsui
University of Brasilia, Brasilia, Brazil;
Grad. Sch. of Comprehensive Human Sci., Univ of Tsukuba, Japan; Neurosci. Res. Inst., AIST, Ibaraki, Japan
Recent studies revealed that single neurons in dorsal raphe nucleus (DR) carry information on reward. We reported that the neurons in/around DR of rhesus monkey showed responses that were reward contingent during multi-trial reward schedules (JNNS meeting in Tokyo, 2008). We now ask whether they encode signals related to reward amount. We manipulated both schedule length (1, 2 and 3 trials) and reward amount (1, 2 and 3 drops of liquid) in modified version of reward schedules. During the task the length and brightness of visual cue indicated the schedule progress and reward amount, respectively. The error rate was modulated both by schedule progress and reward amount. We recorded 12 neurons from DR. The activity of 6/12 neurons were significantly different in relation to reward amount, suggesting that DR might have information related to reward amount. doi:10.1016/j.neures.2009.09.1029
P2-n16 Effects of reward proximity and amount on neural activity in the rostral anterior cingulate cortex Koji Toda 1,2 , Takashi Mizuhiki 1 , Yasuko Sugase-Miyamoto 3 , Kiyonori Inaba 1 , Shigeru Ozaki 1 , Barry J. Richmond 4 , Munetaka Shidara 1 1
Grad Sch Comprehensive Human Science, Univ Tsukuba, Japan; Japan; 3 Neurosci Res Inst, AIST, Japan; 4 NIMH, NIH, USA
2
JSPS,
Anterior cingulate cortex (ACC) is thought to be related to reward expectancy. We ask how sub-regions of the ACC differ in their reward representation. To study two