- Email: [email protected]
PCR detection of genetically modified maize and soya in mildly and highly processed foods
S96
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
of 2 months, in 2011. Ten samples from each store were collected and transferred to the laboratory. For Salmonella isolation, conventional culture methods were performed. For confirmation and S. enteritidis serovar determination, a PCR reaction assay was used, with Prot6E gene primers (Prot6e-5 and Prot6e-6), specific for S. enteritidis serovar. 37 samples out of 100 showed positive results for Salmonella spp. presence, while 24 out of them were contaminated with Salmonella enteritidis. The main conclusion was that table eggs represent a potential risk considering salmonellosis, especially due to the existence of S. enteritidis serovar. doi:10.1016/j.copbio.2011.05.298
threshold level. However, the current situation about the foods we consume is pretty uncertain. In this present study detection of GM maize and soya in food products obtained from local retail shops was performed. The samples included, maize flour, soya flour, canned maize, bread, cake, meat products and similar soya and maize containing foods. The samples were analyzed for CaMV 35S promoter and nos terminator sequences which are the most common sequences inserted in GM crops. The samples positive for both or either sequences were evaluated as GM positive. The negative maize and soya samples were detected for zein and lectin respectively for the determination of detectible DNA in the sample. The results indicated that 10 of 30 samples detected, were positive for either or both of the novel sequences.
I23
doi:10.1016/j.copbio.2011.05.300
Relationship of somatic cell count with milk yield and composition in Holstein and Romanian Spotted cow crossbreed population
I25
Lucian Ionel Ilie 1 , Anca Maria Galis 2 , Laurentiu Tudor 1 1
Veterinary Medicine Department, University of Agricultural Sciences and Veterinary Medicine of Bucharest, Romania 2 Animal Science Department, University of Agricultural Sciences and Veterinary Medicine of Bucharest, Romania E-mail address: [email protected] (L.I. Ilie)
The objective of this study was to analyze the relationship between somatic cell count (SCC), milk yield (MY), fat and protein percentage (FP/PP), fat and protein yield (FY/PY), using analysis of variance and correlation analysis in Romanian crossbreed cows. The records were obtained from ten commercial herds, located in different counties near Bucharest, during September 2010 and January 2011. The cows presented different ages, being at first, second, third, fourth or later parity, this specific fact being used as a variation factor considering the studied parameters. MY, FP, PP, FY and PY showed variations, with the highest values for cows at first and second parity, while the lowest values were associated with cows at fourth and later parity, the situation for SCC being totally opposite. Analysis of variance showed highly significant effect of SCC values on MY and composition. The correlations were highly negative between SCC, MY and PY, and highly positive for SCC, FY, FP and PP. There was no significant relationship between FP and PY (P > 0.05). The results of the present study provide relevant data for assessing milk production in an area considered important for milk supply in Bucharest. doi:10.1016/j.copbio.2011.05.299
I24
Encapsulation of food flavors via coacervation method Perihan Merve Buldur, Fatma Nese Kok Molecular Biology and Genetics Department, Istanbul Technical University, Istanbul, Turkey E-mail address: [email protected] (P.M. Buldur) Flavors are used in many areas, such as food, cosmetic, pharmaceutical and detergent industries to increase taste and/or odor. These highly valuable compounds lose their effect beginning from the production step due to their volatile and oxidation labile nature. The decreases in properties continue during the transfer and storage of the product. Encapsulation of flavors into different materials (e.g. gelatin, gum arabic, whey proteins, and pectin) is a well known method to solve this problem. In this study, the potential of coacervation method in the efficient encapsulation of food flavors were studied. The model flavors, d-limonene and 2-phenylethanol, were encapsulated into different ratio of gum arabic/gelatin mixtures. Prepared microcapsules were crosslinked by a natural, non-toxic agent, namely genipin. The microbeads were then collected and dried by lyophilization. Preparation conditions and different crosslinking agent concentrations were studied for their effect on coacervation properties and flavor retention during drying and storage. Microbeads were visualized in fluorescence microscopy using nile red as lipid staining dye. It was seen that increasing the gelatin and gum arabic concentration in the medium to more than 40% and 50%, respectively, caused aggregation of the newly formed microbeads. Optimum ratio was found to be 2:3 for gum arabic/gelatin. doi:10.1016/j.copbio.2011.05.301
PCR detection of genetically modified maize and soya in mildly and highly processed foods
I26
Ozge Ozgen Arun, Funda Yilmaz
Selection of lactic acid bacteria strains producing exopolysaccharides
Besin Hijyeni ve Teknolojisi Anabilim Dali, Veteriner Fakultesi, Istanbul, Turkey
Ionela Sarbu 1 , Tatiana Vassu 1 , Ileana Stoica 1 , Emanuel Vamanu 2 , Diana Roxana Pelinescu 1
E-mail address: [email protected] (O.O. Arun) Despite the announced advances, genetically modified organisms (GMO) have received a very strong consumer reaction since they were first commercialized. For this purpose numerous countries set up their official regulations on labeling of GMOs and GMO derivate foods. Finally regulation on traceability and labeling of GM foods, have come into force in our country. The Regulation stipulates the labeling of foods that contain GM material above the
1
Genetics department, Faculty of Biology, Bucharest, Romania Biotechnology Department, Faculty of Biotechnology, University of Agronomical Science and Veterinary Medicine, Bucharest, Romania 2
E-mail address: [email protected] (D.R. Pelinescu) Production of exopolysaccharides (EPS) generated a high interest due to their important industrial application and beneficial effects on human health. Main goal of our study was selection of
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
of 2 months, in 2011. Ten samples from each store were collected and transferred to the laboratory. For Salmonella isolation, conventional culture methods were performed. For confirmation and S. enteritidis serovar determination, a PCR reaction assay was used, with Prot6E gene primers (Prot6e-5 and Prot6e-6), specific for S. enteritidis serovar. 37 samples out of 100 showed positive results for Salmonella spp. presence, while 24 out of them were contaminated with Salmonella enteritidis. The main conclusion was that table eggs represent a potential risk considering salmonellosis, especially due to the existence of S. enteritidis serovar. doi:10.1016/j.copbio.2011.05.298
threshold level. However, the current situation about the foods we consume is pretty uncertain. In this present study detection of GM maize and soya in food products obtained from local retail shops was performed. The samples included, maize flour, soya flour, canned maize, bread, cake, meat products and similar soya and maize containing foods. The samples were analyzed for CaMV 35S promoter and nos terminator sequences which are the most common sequences inserted in GM crops. The samples positive for both or either sequences were evaluated as GM positive. The negative maize and soya samples were detected for zein and lectin respectively for the determination of detectible DNA in the sample. The results indicated that 10 of 30 samples detected, were positive for either or both of the novel sequences.
I23
doi:10.1016/j.copbio.2011.05.300
Relationship of somatic cell count with milk yield and composition in Holstein and Romanian Spotted cow crossbreed population
I25
Lucian Ionel Ilie 1 , Anca Maria Galis 2 , Laurentiu Tudor 1 1
Veterinary Medicine Department, University of Agricultural Sciences and Veterinary Medicine of Bucharest, Romania 2 Animal Science Department, University of Agricultural Sciences and Veterinary Medicine of Bucharest, Romania E-mail address: [email protected] (L.I. Ilie)
The objective of this study was to analyze the relationship between somatic cell count (SCC), milk yield (MY), fat and protein percentage (FP/PP), fat and protein yield (FY/PY), using analysis of variance and correlation analysis in Romanian crossbreed cows. The records were obtained from ten commercial herds, located in different counties near Bucharest, during September 2010 and January 2011. The cows presented different ages, being at first, second, third, fourth or later parity, this specific fact being used as a variation factor considering the studied parameters. MY, FP, PP, FY and PY showed variations, with the highest values for cows at first and second parity, while the lowest values were associated with cows at fourth and later parity, the situation for SCC being totally opposite. Analysis of variance showed highly significant effect of SCC values on MY and composition. The correlations were highly negative between SCC, MY and PY, and highly positive for SCC, FY, FP and PP. There was no significant relationship between FP and PY (P > 0.05). The results of the present study provide relevant data for assessing milk production in an area considered important for milk supply in Bucharest. doi:10.1016/j.copbio.2011.05.299
I24
Encapsulation of food flavors via coacervation method Perihan Merve Buldur, Fatma Nese Kok Molecular Biology and Genetics Department, Istanbul Technical University, Istanbul, Turkey E-mail address: [email protected] (P.M. Buldur) Flavors are used in many areas, such as food, cosmetic, pharmaceutical and detergent industries to increase taste and/or odor. These highly valuable compounds lose their effect beginning from the production step due to their volatile and oxidation labile nature. The decreases in properties continue during the transfer and storage of the product. Encapsulation of flavors into different materials (e.g. gelatin, gum arabic, whey proteins, and pectin) is a well known method to solve this problem. In this study, the potential of coacervation method in the efficient encapsulation of food flavors were studied. The model flavors, d-limonene and 2-phenylethanol, were encapsulated into different ratio of gum arabic/gelatin mixtures. Prepared microcapsules were crosslinked by a natural, non-toxic agent, namely genipin. The microbeads were then collected and dried by lyophilization. Preparation conditions and different crosslinking agent concentrations were studied for their effect on coacervation properties and flavor retention during drying and storage. Microbeads were visualized in fluorescence microscopy using nile red as lipid staining dye. It was seen that increasing the gelatin and gum arabic concentration in the medium to more than 40% and 50%, respectively, caused aggregation of the newly formed microbeads. Optimum ratio was found to be 2:3 for gum arabic/gelatin. doi:10.1016/j.copbio.2011.05.301
PCR detection of genetically modified maize and soya in mildly and highly processed foods
I26
Ozge Ozgen Arun, Funda Yilmaz
Selection of lactic acid bacteria strains producing exopolysaccharides
Besin Hijyeni ve Teknolojisi Anabilim Dali, Veteriner Fakultesi, Istanbul, Turkey
Ionela Sarbu 1 , Tatiana Vassu 1 , Ileana Stoica 1 , Emanuel Vamanu 2 , Diana Roxana Pelinescu 1
E-mail address: [email protected] (O.O. Arun) Despite the announced advances, genetically modified organisms (GMO) have received a very strong consumer reaction since they were first commercialized. For this purpose numerous countries set up their official regulations on labeling of GMOs and GMO derivate foods. Finally regulation on traceability and labeling of GM foods, have come into force in our country. The Regulation stipulates the labeling of foods that contain GM material above the
1
Genetics department, Faculty of Biology, Bucharest, Romania Biotechnology Department, Faculty of Biotechnology, University of Agronomical Science and Veterinary Medicine, Bucharest, Romania 2
E-mail address: [email protected] (D.R. Pelinescu) Production of exopolysaccharides (EPS) generated a high interest due to their important industrial application and beneficial effects on human health. Main goal of our study was selection of