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Peliosis hepatis mimicking a large solitary tumor of the liver
234
Papers read by title
I c11/015
A MATHEMATICAL
FORMULA TO ALLOW COMPARISON OF BIOCHEMICAL
DATA FROM DIFFERENT LABORATORIES FOR HEPATOLOGICAL RESEARCH
PELIOSIS SOLITARY
I
HEPATIS MIMICKING TUMOR OF THE LIVER
A
LARGE
MS. Bassoni, P. Loria, S. Azzoni, M. Bertolotti and N. Carulli Department of Internal Medicine, University of Modena, Modena, Italy Hepatological research often deals with changes induced by treatment on biochemical parameters. In multicentric studies biochemical determinations are usually performed in different places. Since every laboratory gives a different normal range for parameters, it is not advisable to compare data from different laboratories without any algebraical correction. In this context measured data are generally expressed as a multiple of the superior limit of the normal range. This method is not mathematically
correct because it does not consider the
“scaling factor”, related to the different amplitude of the normal range. For this reason we propose a new equation that allows researchers to correct biochemical data coming from different laboratories. The equation is
resulf=
We report two cases of Peliisis Hepatis. The first case refers to a 63 years old man who underwent right nephrectomy for transitional cell carcinoma. Before surgery spiral computed tomography (PICKER 5000V) revealed a large space occupying lesion, 10X12 cm in the right hepatic lobe with unhomogeneous low density lesion with peripheral enhancement after contrast infusion. On the basis of this finding, the most likely diagnosis was that of a benign neoplasmatic mass. The histological examination of a liver biopsy taken during surgery was compatible with Peliosis Hepatis. The second case concerns a 80 years okl man who was admitted to our hospital because of anemia. The spiral CT revealed .a large unhomogeneous space occupying lesion 15X16 cm in the right hepatic lobe with findings identical to the ones of the first case. These findings were considered pathognomonic for Peliisis Hepatis. Liver biopsy confirmed the diagnosis. Conclusions: We report these cases taking in consideration that peliisis hepatis is, according to the current literature, a very rare entity causing diagnostic problems, specially when it appears as a single large space occupying mass.
(vulue-minr~,).(max,,-min,,)+min ) (maxlab- min,, W,
where “value”is the value determined in the patient, rnim#b and mausa are respectively the minimum and maximum value of the normal range in the laboratory doing the test, while millnr and maxnf are those of the normal range chosen as reference. That formula can be easily used by a computer. The application
Department of Radiology, *Ist Department of Internal Medicine, **Department of Pathology, Sismanogliin General Hospital, Athens, Greece
of the formula proposed allows data conversion avoiding a
systematical error that can be committed if the scaling factor is not considered. It may therefore be recommended when handling data in hepatological research.
RE?UTILIZATION OF “C-THYMlDlNE YOUNG AND ADULT MICE
M THE LIVER OF
H. iivn& P. iivnC*. L. Sobotka*. Z. Cervinkova V. PaliEka* Department of Physiology and *Institute of Clinical Biochemistry, Charles University, Faculty of Medicine, Hradec KrBlovC, Czech Republic The aim was to study reutilization of DNA nucleotide bases in the liver and spleen of young and adult mice. Methods: The experiments were performed on 128 mice, each group had 16 animals: 8 young (body weight 20-27 g) and 8 adult (42-49 g). All animals were given 14C-thymidine (Th) s. c. (3 pCi every 24 hours during 72 hours). Control groups were sacrificed 24 hours (INT24) and/or 72 hours after finishing of Th application (INT72). Laparotomy (LAP), partial hepatectomy (PH), splenectomy (SE), PH + SE PHSE), LAP + hydrocortisone application (1.3 mg per 10 g of body weight; LAPH), PH + hydrocortisone application (PHH) were performed 24 h after finishing of Th application in experimental groups. Animals were sacrificed 48 h after operations. Specific activity of liver and spleen DNA (SADNA) were estimated. Data was statistically treated using Jandel Scientific software. Results: Selected results are presented as mean and SEM in dpm lo3 per mg of DNA. There was no difference in liver SADNA between controls and experimental groups in young mice (all results were between 6.06 and 6.48). Liver SADNA in adult groups were: 3.15ti.6 in INT24, 2.03ti.15 in INT72, 2.1i0.18 in LAP, 4.18kO.42 in PH, 2.1f0.24 in LAPH, 3.72+0.40 in PHH, 2.54ti.30 in SE and 4.2f0.42 PHSE group. Conclusion: There are evident differences in reutilization of DNA nucleotide bases in young and adult animals. The redistribution of 14C-thymidine is more evident in adult mice, especially after PF,. and is not ‘suppressed by SE and hydrocortisone admuustration.
PREVALENCE OF HGV IN HEALTH CARE WORKERS G.Lun&i. R.Cardone. A.Orlandi. M.Oanissanti. M.Parziale*, A.Paaano. Laboratorio di C’irologia, *Servizio di Medicina Prevenfiva. IRCCS Ospedale Maggiore, Milano, ItaIy. Objective: To estimate the prevalence of HGV, a recently identified member of the Flaviviridae, in a group at potential high risk of blood borne infections. Study desitzn: 600 health care workers from the Ospedale Maggiore of Milan were investigated for the presence of HGV antibodies to the E2 protein (anti HGenv, Boehringer Mannheim) and HGV RNA by PCR. Results: 101 (16.8%) people were found to be anti HGV positive and 25 (4.1%) HGV RNA positive. In the same group the HCV prevalene was 3.3%. Conclusion: Our data demonstre an elevated rate of HGV prevalence among health workers, increasing with age, years and unit of work (haemodialysis, surgery). Although the pathogenicity of Hepatitis G virus is still unclear, the HGV prevalence can be studied as an effective marker of exposition to blood borne infections.
Papers read by title
I c11/015
A MATHEMATICAL
FORMULA TO ALLOW COMPARISON OF BIOCHEMICAL
DATA FROM DIFFERENT LABORATORIES FOR HEPATOLOGICAL RESEARCH
PELIOSIS SOLITARY
I
HEPATIS MIMICKING TUMOR OF THE LIVER
A
LARGE
MS. Bassoni, P. Loria, S. Azzoni, M. Bertolotti and N. Carulli Department of Internal Medicine, University of Modena, Modena, Italy Hepatological research often deals with changes induced by treatment on biochemical parameters. In multicentric studies biochemical determinations are usually performed in different places. Since every laboratory gives a different normal range for parameters, it is not advisable to compare data from different laboratories without any algebraical correction. In this context measured data are generally expressed as a multiple of the superior limit of the normal range. This method is not mathematically
correct because it does not consider the
“scaling factor”, related to the different amplitude of the normal range. For this reason we propose a new equation that allows researchers to correct biochemical data coming from different laboratories. The equation is
resulf=
We report two cases of Peliisis Hepatis. The first case refers to a 63 years old man who underwent right nephrectomy for transitional cell carcinoma. Before surgery spiral computed tomography (PICKER 5000V) revealed a large space occupying lesion, 10X12 cm in the right hepatic lobe with unhomogeneous low density lesion with peripheral enhancement after contrast infusion. On the basis of this finding, the most likely diagnosis was that of a benign neoplasmatic mass. The histological examination of a liver biopsy taken during surgery was compatible with Peliosis Hepatis. The second case concerns a 80 years okl man who was admitted to our hospital because of anemia. The spiral CT revealed .a large unhomogeneous space occupying lesion 15X16 cm in the right hepatic lobe with findings identical to the ones of the first case. These findings were considered pathognomonic for Peliisis Hepatis. Liver biopsy confirmed the diagnosis. Conclusions: We report these cases taking in consideration that peliisis hepatis is, according to the current literature, a very rare entity causing diagnostic problems, specially when it appears as a single large space occupying mass.
(vulue-minr~,).(max,,-min,,)+min ) (maxlab- min,, W,
where “value”is the value determined in the patient, rnim#b and mausa are respectively the minimum and maximum value of the normal range in the laboratory doing the test, while millnr and maxnf are those of the normal range chosen as reference. That formula can be easily used by a computer. The application
Department of Radiology, *Ist Department of Internal Medicine, **Department of Pathology, Sismanogliin General Hospital, Athens, Greece
of the formula proposed allows data conversion avoiding a
systematical error that can be committed if the scaling factor is not considered. It may therefore be recommended when handling data in hepatological research.
RE?UTILIZATION OF “C-THYMlDlNE YOUNG AND ADULT MICE
M THE LIVER OF
H. iivn& P. iivnC*. L. Sobotka*. Z. Cervinkova V. PaliEka* Department of Physiology and *Institute of Clinical Biochemistry, Charles University, Faculty of Medicine, Hradec KrBlovC, Czech Republic The aim was to study reutilization of DNA nucleotide bases in the liver and spleen of young and adult mice. Methods: The experiments were performed on 128 mice, each group had 16 animals: 8 young (body weight 20-27 g) and 8 adult (42-49 g). All animals were given 14C-thymidine (Th) s. c. (3 pCi every 24 hours during 72 hours). Control groups were sacrificed 24 hours (INT24) and/or 72 hours after finishing of Th application (INT72). Laparotomy (LAP), partial hepatectomy (PH), splenectomy (SE), PH + SE PHSE), LAP + hydrocortisone application (1.3 mg per 10 g of body weight; LAPH), PH + hydrocortisone application (PHH) were performed 24 h after finishing of Th application in experimental groups. Animals were sacrificed 48 h after operations. Specific activity of liver and spleen DNA (SADNA) were estimated. Data was statistically treated using Jandel Scientific software. Results: Selected results are presented as mean and SEM in dpm lo3 per mg of DNA. There was no difference in liver SADNA between controls and experimental groups in young mice (all results were between 6.06 and 6.48). Liver SADNA in adult groups were: 3.15ti.6 in INT24, 2.03ti.15 in INT72, 2.1i0.18 in LAP, 4.18kO.42 in PH, 2.1f0.24 in LAPH, 3.72+0.40 in PHH, 2.54ti.30 in SE and 4.2f0.42 PHSE group. Conclusion: There are evident differences in reutilization of DNA nucleotide bases in young and adult animals. The redistribution of 14C-thymidine is more evident in adult mice, especially after PF,. and is not ‘suppressed by SE and hydrocortisone admuustration.
PREVALENCE OF HGV IN HEALTH CARE WORKERS G.Lun&i. R.Cardone. A.Orlandi. M.Oanissanti. M.Parziale*, A.Paaano. Laboratorio di C’irologia, *Servizio di Medicina Prevenfiva. IRCCS Ospedale Maggiore, Milano, ItaIy. Objective: To estimate the prevalence of HGV, a recently identified member of the Flaviviridae, in a group at potential high risk of blood borne infections. Study desitzn: 600 health care workers from the Ospedale Maggiore of Milan were investigated for the presence of HGV antibodies to the E2 protein (anti HGenv, Boehringer Mannheim) and HGV RNA by PCR. Results: 101 (16.8%) people were found to be anti HGV positive and 25 (4.1%) HGV RNA positive. In the same group the HCV prevalene was 3.3%. Conclusion: Our data demonstre an elevated rate of HGV prevalence among health workers, increasing with age, years and unit of work (haemodialysis, surgery). Although the pathogenicity of Hepatitis G virus is still unclear, the HGV prevalence can be studied as an effective marker of exposition to blood borne infections.