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Performance of pediatric nucleus multichannel cochlear implant users with partial electrode insertion
P150
Otolaryngology Head and Neck Surgery August 1995
Scientific Sessions- - Tuesday
pared with pediatric cholesteatomas with use of cell culture analysis, RT-PCR analysis, and immunohistochemistry. Cell cultures of fibroblasts from the matrix of 12 cholesteatomas were evaluated for their ability to migrate, attach to, and invade basement membranes. The adult and pediatric cultures were equally invasive. RT-PCR analysis of 24 cholesteatomas was performed and revealed no difference in the cytokine/lymphokine profile present in the adult and pediatric cholesteatomas. Immunohistochemical expression Of cell surface peptidases was found to be similar in five adult and in six pediatric cholesteatomas. We propose that the pediatric cholesteatoma is not different from the adult cholesteatoma at the cellular level. Poster 70
C-Jun and p53 in Human Middle Ear Cholesteatoma Role in Keratinocyte Proliferation, Differentiation, and Programmed Ceil Death HIDEO SHINODA, MD (presenter), and CHENG-CHUN HUANG, PHD, New York, N.Y.
Cholesteatoma is usually associated with an inflammatory reaction that occurs in the middle ear cavity, leading to proliferation and differentiation of keratinocytes in response. Moreover, keratin debris in the outer epithelial layer in cholesteatoma maintains the inflammatory state of the middle ear cavity. In this study we investigated cellular differentiation and proliferation of keratinocytes during the growth of cholesteatoma by immunohistochemical staining with polyclonal anti-c-jun protein and monoclonal anti-p53 protein antibody, c-Jun functions as a transcription factor. P53 protein is a negative regulator of cellular proliferation related to the apoptotic pathway, inducing DNA damage. The cholesteatoma tissue incubated with anti-c-jun antibody showed the staining of all layers of cholesteatoma epithelium. In normal skin, c-jun was lightly stained in the basal layer only. The immunolocalization shows that p53 protein was present in the nuclei of keratinocytes in granular layers of cholesteatoma epithelium. Normal external ear canal skins and normal human skins were slightly stained in the granular layer of epidermis. The present finding suggests that cjun and p53 protein could serve as markers for kertinocyte proliferation, differentiation, and apoptosis during development of cholesteatoma. Poster 71
Early Speech Changes in Children with Mullichannel Cochlear Implants GABRIEL O. TE, MD (presenter), FRANKLIN M. RIZER,MD, ARNOLD G. SCHURING, MD, and WILLIAM H. LIPPY,MD, Warren, Ohio
Multiple studies have demonstrated that cochlear implants provide an improved auditory signal and enhance the development of speech perception and production skills for profoundly deaf children. However, exactly when these early speech skills begin to occur remains unclear. To explore this
issue, we followed four prelingually deaf children who received implants consecutively within a month for a period of 1 year with particular attention to the first few months of rehabilitation. We found immediate speech gains as early as the first day of implant tune-up. Speech production continued to improve rapidly throughout the first 4 months but exhibited a generally slower rate of progress in some of the speech production skills at 1 year. We also found vowel production skills to be the easiest to achieve with word pattern recognition and consonant voicing of intermediate difficulty. Consonant placing and manner of consonant production were the hardest measures to achieve. Speech perception tested at 1 year after implant markedly improved in three of the four children. These early speech changes stress the need to maximize the capability of the cochlear implant by instituting immediate and intensive speech rehabilitation efforts for prelingually deaf children. Poster 72
Performance of Pediatric Nucleus Multichannel Cochlear Implant Users With Partial Electrode Insertion MARK SEHGAL, MD (presenter), KAREN ILERKIRK, PHD, and RICHARD T, MIYAMOTO. MD, Indianapolis, Ind,
This investigation examines the speech perception performance of six children with ossified cochleas who received partial insertions of the Nucleus 22 channel cochlear implant. All the partial insertion subjects were fit with their Nucleus cochlear implant between the ages of 2 to 5 years and have used their device for at least 1.5 years; two of these subjects had used their devices even longer, up to 4.5 years. The partial insertion subjects' preimplant and 1.5 years postimplant performance on a battery of speech perception tests was compared with the average performance of agematched control subjects who received full insertions of their electrode array. More extended comparisons were made for the two partial insertion subjects who had used their cochlear imptants for a longer period of time. The results revealed that the subjects with partial electrode insertions perform similarly to the control group at preimplant and 1.5 years postimplant. Furthermore, the partial insertion subjects showed continued improvements in speech perception performance with increased device experience past 1.5 years, again similar to the full-insertion control group. The present results suggest that partial insertion of a multichannel implant device is an appropriate and feasible approach to the surgical management and auditory rehabilitation of children with complete ossification of the cochlea. (Work supported by NI-NIDCD.)
Otolaryngology Head and Neck Surgery August 1995
Scientific Sessions- - Tuesday
pared with pediatric cholesteatomas with use of cell culture analysis, RT-PCR analysis, and immunohistochemistry. Cell cultures of fibroblasts from the matrix of 12 cholesteatomas were evaluated for their ability to migrate, attach to, and invade basement membranes. The adult and pediatric cultures were equally invasive. RT-PCR analysis of 24 cholesteatomas was performed and revealed no difference in the cytokine/lymphokine profile present in the adult and pediatric cholesteatomas. Immunohistochemical expression Of cell surface peptidases was found to be similar in five adult and in six pediatric cholesteatomas. We propose that the pediatric cholesteatoma is not different from the adult cholesteatoma at the cellular level. Poster 70
C-Jun and p53 in Human Middle Ear Cholesteatoma Role in Keratinocyte Proliferation, Differentiation, and Programmed Ceil Death HIDEO SHINODA, MD (presenter), and CHENG-CHUN HUANG, PHD, New York, N.Y.
Cholesteatoma is usually associated with an inflammatory reaction that occurs in the middle ear cavity, leading to proliferation and differentiation of keratinocytes in response. Moreover, keratin debris in the outer epithelial layer in cholesteatoma maintains the inflammatory state of the middle ear cavity. In this study we investigated cellular differentiation and proliferation of keratinocytes during the growth of cholesteatoma by immunohistochemical staining with polyclonal anti-c-jun protein and monoclonal anti-p53 protein antibody, c-Jun functions as a transcription factor. P53 protein is a negative regulator of cellular proliferation related to the apoptotic pathway, inducing DNA damage. The cholesteatoma tissue incubated with anti-c-jun antibody showed the staining of all layers of cholesteatoma epithelium. In normal skin, c-jun was lightly stained in the basal layer only. The immunolocalization shows that p53 protein was present in the nuclei of keratinocytes in granular layers of cholesteatoma epithelium. Normal external ear canal skins and normal human skins were slightly stained in the granular layer of epidermis. The present finding suggests that cjun and p53 protein could serve as markers for kertinocyte proliferation, differentiation, and apoptosis during development of cholesteatoma. Poster 71
Early Speech Changes in Children with Mullichannel Cochlear Implants GABRIEL O. TE, MD (presenter), FRANKLIN M. RIZER,MD, ARNOLD G. SCHURING, MD, and WILLIAM H. LIPPY,MD, Warren, Ohio
Multiple studies have demonstrated that cochlear implants provide an improved auditory signal and enhance the development of speech perception and production skills for profoundly deaf children. However, exactly when these early speech skills begin to occur remains unclear. To explore this
issue, we followed four prelingually deaf children who received implants consecutively within a month for a period of 1 year with particular attention to the first few months of rehabilitation. We found immediate speech gains as early as the first day of implant tune-up. Speech production continued to improve rapidly throughout the first 4 months but exhibited a generally slower rate of progress in some of the speech production skills at 1 year. We also found vowel production skills to be the easiest to achieve with word pattern recognition and consonant voicing of intermediate difficulty. Consonant placing and manner of consonant production were the hardest measures to achieve. Speech perception tested at 1 year after implant markedly improved in three of the four children. These early speech changes stress the need to maximize the capability of the cochlear implant by instituting immediate and intensive speech rehabilitation efforts for prelingually deaf children. Poster 72
Performance of Pediatric Nucleus Multichannel Cochlear Implant Users With Partial Electrode Insertion MARK SEHGAL, MD (presenter), KAREN ILERKIRK, PHD, and RICHARD T, MIYAMOTO. MD, Indianapolis, Ind,
This investigation examines the speech perception performance of six children with ossified cochleas who received partial insertions of the Nucleus 22 channel cochlear implant. All the partial insertion subjects were fit with their Nucleus cochlear implant between the ages of 2 to 5 years and have used their device for at least 1.5 years; two of these subjects had used their devices even longer, up to 4.5 years. The partial insertion subjects' preimplant and 1.5 years postimplant performance on a battery of speech perception tests was compared with the average performance of agematched control subjects who received full insertions of their electrode array. More extended comparisons were made for the two partial insertion subjects who had used their cochlear imptants for a longer period of time. The results revealed that the subjects with partial electrode insertions perform similarly to the control group at preimplant and 1.5 years postimplant. Furthermore, the partial insertion subjects showed continued improvements in speech perception performance with increased device experience past 1.5 years, again similar to the full-insertion control group. The present results suggest that partial insertion of a multichannel implant device is an appropriate and feasible approach to the surgical management and auditory rehabilitation of children with complete ossification of the cochlea. (Work supported by NI-NIDCD.)