Perfusion culture of hybridoma cells recycling higher-molecular-weight components

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Abstracts of the Articles Printed in Hakkokogaku Kaishi

A bacterium, R C O - 4 M , that c a n grow on cyclohexylacetic acid as the sole carbon source was isolated from soil by e n r i c h m e n t cultures. T h e isolate was identified as a species of the genus Micrococcus by taxonomic studies. T h e optimal culture conditions of this organism were as follows: the growth m e d i u m consisted of cyclohexylacetic acid 0.15%, K N O a 0.25%, K H 2 P O a 0.1%, MgSO4"7H~O 0.002%, FeCla.6H~O 0.005%, CaC12.2H~O 0.002%, a n d yeast extract 0.01%, p H 8.0. T h e culture was incubated at 30°C for 3-7 d on a shaker. Strain R C O - 4 M grew on a n u m b e r of types of alicyclic compounds. A m o n g the alicyclic carboxylic acids tested, cyelohexylacetic acid a n d cyclohexylbutyric acid were the best substrates. T h e o r g a n i s m grew on cyclohexylpropionic acid, a n d grew a little on both cyclohexanecarboxylic acid a n d cyclopentanecarboxylic acid. This isolate also grew on cyclohexanone a n d cyclohexanol. Strain R C O - 4 M grew well on all of the n-alkylcyclohexanes h a v i n g 6-14 carbon atoms in the n-alkyl group, after a long lag time. * Corresponding author

[J. Ferment. Technol.,

Perfusion Culture of Hybridoma Cells Recycling Higher,Molecular-Weight Components. MICHIYUKI TOKASHIKI, ~ KIMIHIKO I-IAMAMOTO, a n d YOSHIHARU TAKAZAWA (Tokyo Research Center,

Biotechnology Research Laboratory, Teijin Limited, 4-3-2 Asahigaoka, Hino, Tokyo 191, Japan) H a k k o k o g a k u 66: 31-35. 1988. A perfusion culture was developed in which the cell-free culture s u p e r n a t a n t was ultrafiltered, a n d the retentate was recycled to the culture vessel. O n e m o u s e - m o u s e h y b r i d o m a line a n d three m o u s e - h u m a n h y b r i d o m a lines were cultured by this method. I n the culture of the m o u s e - m o u s e h y b r i d o m a 4C10B6 line using s e r u m s u p p l e m e n t e d m e d i u m , the concentration of monoclonal antibody in the culture vessel was increased in proportion to culture time, b u t cell density decreased as time passed. I n the cultures of 3 m o u s e - h u m a n h y b r i d o m a lines using serum-free m e d i u m , the concentrations of monoclonal antibodies in culture vessels increased in proportion to culture time to 20 times that of a conventional perfusion culture, a n d viable cell densities were not less t h a n those of conventional perfusion cultures. * Corresponding author

Vol. 66, 1988]

Instructions to Authors

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Instructions to Authors for the Journal of Fermentation Technology General Policy The Journal of Fermentation Technology is devoted to the advancement and dissemination of knowledge concerning applied biology, which covers all the technological aspects of microbiology, plant and animal cell culture, enzymology, genetics, in addition to fermentation technology, biochemical engineering, food technology and the related field. Basic research which is intended for application is also welcome. Research articles submitted must be reports of unpublished original research, which are not being considered for publication elsewhere.

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