- Email: [email protected]
Pericytes of ciliary processes capillaries in rats SEM study
Monday, 5:30-100 P.M., Sep 21, 1992 Palazu, Dei Congressi
X ICER Abstracts 137
10 ACTION
OF
OYNAHI
AN
NA-K-ATPASE
INHIBITOR
ON
AQUEOUS
CS
--Rodrigues,
M.L.V.,
Department ty of Brazil.
of lledicine
Althoug upon
glycosides
aqueous
humor,
dictory
results. this
the
of
group
the
eye
of
was
groups
20
punctured
registered queous
humor
was
the
results,
subject
7.0
and
the
show
-
received a
with of
cnamber
ar
intraocu
n
hydrody
by
the
contra
compared
anterior
The estimated
act of
rabbits
were
The
manometrically.
FaculPaulc-
3ao
theoretically,
this
and
rabbits.
__
hydrod\;namics
of
Digoxin,
E.
of
and on
four
doses
control
studies
siudy,
different
can,
pressure
Romao A-
Otolaringology University
Preto,
intraocular
A.
--.L--Land
cardiac
the
In
Lauretti-Filho -Ophthalmology Ribeirao
infusion
pressure
was
amic5
of
the
under
constant
a-
pressure. From tally
we
significant
concluded
that
differences
were
observed
between
with
received
the
in the
control
Na-K-ATPase
-
intra
no
statisti-
cular
0
group
and
LEVEL
OF
pressure the
groups
inhibitor.
138
11 CARBONIC
ANHIDRASE
AQUEOUS
HU1:OR
Araujo
Zinc,
A.C.N.,
the It with
human In
drase
H.L.V.,
is
body
a
almost of
most
is
I
Sou~a,~-~_,
PERICYTES
I
Dept.
a
several
Its humor,
of
growth
carbonic
of
of
carbonic
inhibition
reduces
and,
in
isoenzyre.
humans,
anhy the
rroocular
subsequently,
We
mor
studied
and
blood.
chlorphenamide not
the
inhibitor)
in
effect on
Results
of the
showed
interferes aqueous
dichlorphenamide
level
on
of that, zinc
(carbonic
zinc
in
for
aqueovs
our
amount
in
hu-
sample,
di -
blood,
T.Tenkova’, of Anatomy
PROCESSES CAPILLARIES IN RATS SEM STUDY M.Petrov’, T.Fujiwara’, Sh.Tsukahara3 and Histology, Varna Medical University’, Bulgaria of Medicine, Lab. Animal Center2, Dept. of Yamanashi Medical College3 JAPAN
For the first time the perycites (P) of ciliary processes (CP) capillaries were demonstrated scanning electron microscopy. Their surface view was visualized after removal of ciliary epithelium and stromel connective tissue by 8N HCL digestion. The P of subepithelial capillaries in CP posesses an oval body, long branching processes along the capillary wall or embracing transversly the vessel, organized in a complicated, thick network. The P were frequently observed on the opposite side of the vessels facing the epithelial cells. The postcapillary venule-P compared to that of subepithelial capillaries had a large and more flat tened bodies with thin and branching processes attached tightly to the endothelial cells. Some fenestrations were observed on the pericytic projections too. These P did not show any preference to the site of their location and were situated rather scaresly in comprasion to the P of the subepithelial capillaries. Deep stromal capillary P demonstrated features simillar to the P of lymphatic vessels-an elongated body, parallel to the long axis, with long, thin branching processes. We suggest that CP capillaries had different types of P. These variations seem to be dependent on the location of the capillary bed.
an-.
found
prf25SUre.
anhydrase
OF CILIARY
Ehime School Ophthalmology
ele-
normal
metalloenzyme forms
processes
aqueous
trace
to
component in
pure.
abundant
necessary
essential
under
ciliary
is
second
body,
constitutes
the
duction
the
human
humans.
hydrase the
Rodrigues,
is
vrich
in
141
14
I N
ZINC
E.
merit of
A"!D
3LOOD
AND
Fare,
Romao,
INHIBITION
140
13
A comparision of mitomycin C with 5-fluorouracil as an aqueous protein concentration adjunct to trabeculectomy: change K. Kawase. K. Nishimura. T . Yamamoto, S. Jikihara & Y. School of Kitazawa. Dept. of Gphthalmol., Gifu Univ. Medicine, Gifu-shi, Japan Possible mitomycin C (MMC) over 5superiority of fluorouracil (5-FU) as an adjunct to trabeculectomy has recently been reported by several authors. In order to compared their effects on the blood-aqueous barrier by measuring aqueous protein concentration in human Subjects trabeculectomy cases treated with either drug. were selected from a total body of 51 cases (65 eye*) treated with MMC and 15 cases’ (20 with 5-FU eyes) according to the following selection criteria to match type of glaucoma, postoperative anterior the two groups: chamber depth, age and other factors known to influence aqueous protein concentration. Thirteen eyes of 13 cases and 7 in MMC group (48.7 + 14.3 yrs, mean + SD) eyes of 7 cases in 5-FU group (58.0 + 13.1 yrs) were thus selected. In the MMC group, 0.2mg MMC was applied for 5 minutes intraoperatively. In 5-FU group, 5mg 5-FU W&¶ subconjunctivally injected repeatedly for the postoperative 2 weeks. We measured the aqueous protein concentration preoperatively and 1,2,3,5,7,14,28 days postoperatively with Laser Flare-Cell Meter@(FC-1000, Kowa, Japan). Mean aqueous protein concentration in the MMC group was significantly lower than that of the 5-FU group on the 2nd postoperative day (128.2 mg/dl vs 399.9; pco.05, Mann-Whitney U test). These results suggest that 5-FU has less favorable effects on BAB than MMC in early postoperative period.
IiUtiOR
but
humor.
142
15 AQUALITY
ASSEWOFXWDOnI
ZED CONTROL GLAUCoIII\
OF OPEW-A#GLE Orzalesi N. Rossetti L. Eye Clinic, University of Milan, S.Paolo Hospital, Milan, Italy TREATMKNT
TRIALS
ON
MEDICAL
SOURCE
OF AQUEOUS
Dept. of Ophthalmology, Institute
of
Biomedical
HUMOR
HYDROGEN
Univ. of Maryland,
PEROXIDE
Baltimore,
Maryland
USA
Sciences,
Hydrogen peroxide is one of the central member of the group of active oxygen dexivativc3. It has remluy IKea dcmalstreted tc be plemt in the normal lenses of most of the animal species ia amounts - lO+f. Aqueous humor of patients with some cataracts also have ekvated levels of peroxkkofthesimilarorder. Thesuurceoftheaqwusperox&ofthe cataract patialb, however, remeinl tmlesdval. Tltcsc llnwt@hs were U~touuunineifitcanbcderivedbyalePksse~rnthe eataraetous lenses. After 3, 6 & 11 days of feeding 35% gala&w diet, the animals were anesthetized with kekMneHC!L aad aqueous withdrawn for Hz& analysis. Subsequeotly, the animals were sacriftcul aad lenses isolated and extra&d with hichloroacetic acid (10%). H&J was estimated in the aqwous and lens extract be reacting them with I-“C-aketoglufarie acid and measuring C& formed by dec&oxylation. The levelSOfH*~inhecontrolaquausmdltnswae5f3and190~U) pM. InulcgaIaetmfcdanimrdsPfter11days,tlteaqucouslevcls incmaacdtoIbf4wdthe~s~~dccraasdb69f20IJII. These changestookpke-asafimedonoftimc. Treamwtofgalwosefed euimals with quereihiD (60 mgklayh) and smbieil (2 IQ&/day/rat) preventsdegsiastthe~ intheaqueowmdtbefaUintbelensH,~ contents. Thus, the attenuation of catamct pmva~ts against the A& leakage from the lens. The results indicate that the lens could be a significant swrce of aqueous peroxide.
A meta-analysis and a qualitative overview of published RCTs was undertaken to assess the effectiveness of medical treatment on outcome of patients with POAG. 102 RCTs were published between 1975 and 1991, totalling about 5000 patients. Only 16/102 were properly designed (i.e. an active treatment vs a placebo) to answer the questlnn. Pooling data from suitable studies (~6) a moderate yet statistically significant reduction in mean IOP was shown (-4.9 mmHg). Only 3 RCTs had, however, data on long-term visual field changes and their statistical combination failed to show a significant protective effect (O&0.75.95% C.I.=O.42-1.35) of active treatment. The methodology of the 102 RCTs was also reviewed. The analysis showed serious methodological drawbacks. In 37% RCTs no assessment of visual field abnormalities was made, in 34% RCTs more than 15% patients were lost to follow-up and 53% RCTd excluded them from the final analysis. In addition only 4% RCTs were planned following a priori estimate of sample size, 76% RCTs reported no information on treatment adherence and in 69% trials nt) information about the method of randomization was mentioned. Therefore the vast majority of published trials seems to be of limited value to guide clinical practice.
s.45
X ICER Abstracts 137
10 ACTION
OF
OYNAHI
AN
NA-K-ATPASE
INHIBITOR
ON
AQUEOUS
CS
--Rodrigues,
M.L.V.,
Department ty of Brazil.
of lledicine
Althoug upon
glycosides
aqueous
humor,
dictory
results. this
the
of
group
the
eye
of
was
groups
20
punctured
registered queous
humor
was
the
results,
subject
7.0
and
the
show
-
received a
with of
cnamber
ar
intraocu
n
hydrody
by
the
contra
compared
anterior
The estimated
act of
rabbits
were
The
manometrically.
FaculPaulc-
3ao
theoretically,
this
and
rabbits.
__
hydrod\;namics
of
Digoxin,
E.
of
and on
four
doses
control
studies
siudy,
different
can,
pressure
Romao A-
Otolaringology University
Preto,
intraocular
A.
--.L--Land
cardiac
the
In
Lauretti-Filho -Ophthalmology Ribeirao
infusion
pressure
was
amic5
of
the
under
constant
a-
pressure. From tally
we
significant
concluded
that
differences
were
observed
between
with
received
the
in the
control
Na-K-ATPase
-
intra
no
statisti-
cular
0
group
and
LEVEL
OF
pressure the
groups
inhibitor.
138
11 CARBONIC
ANHIDRASE
AQUEOUS
HU1:OR
Araujo
Zinc,
A.C.N.,
the It with
human In
drase
H.L.V.,
is
body
a
almost of
most
is
I
Sou~a,~-~_,
PERICYTES
I
Dept.
a
several
Its humor,
of
growth
carbonic
of
of
carbonic
inhibition
reduces
and,
in
isoenzyre.
humans,
anhy the
rroocular
subsequently,
We
mor
studied
and
blood.
chlorphenamide not
the
inhibitor)
in
effect on
Results
of the
showed
interferes aqueous
dichlorphenamide
level
on
of that, zinc
(carbonic
zinc
in
for
aqueovs
our
amount
in
hu-
sample,
di -
blood,
T.Tenkova’, of Anatomy
PROCESSES CAPILLARIES IN RATS SEM STUDY M.Petrov’, T.Fujiwara’, Sh.Tsukahara3 and Histology, Varna Medical University’, Bulgaria of Medicine, Lab. Animal Center2, Dept. of Yamanashi Medical College3 JAPAN
For the first time the perycites (P) of ciliary processes (CP) capillaries were demonstrated scanning electron microscopy. Their surface view was visualized after removal of ciliary epithelium and stromel connective tissue by 8N HCL digestion. The P of subepithelial capillaries in CP posesses an oval body, long branching processes along the capillary wall or embracing transversly the vessel, organized in a complicated, thick network. The P were frequently observed on the opposite side of the vessels facing the epithelial cells. The postcapillary venule-P compared to that of subepithelial capillaries had a large and more flat tened bodies with thin and branching processes attached tightly to the endothelial cells. Some fenestrations were observed on the pericytic projections too. These P did not show any preference to the site of their location and were situated rather scaresly in comprasion to the P of the subepithelial capillaries. Deep stromal capillary P demonstrated features simillar to the P of lymphatic vessels-an elongated body, parallel to the long axis, with long, thin branching processes. We suggest that CP capillaries had different types of P. These variations seem to be dependent on the location of the capillary bed.
an-.
found
prf25SUre.
anhydrase
OF CILIARY
Ehime School Ophthalmology
ele-
normal
metalloenzyme forms
processes
aqueous
trace
to
component in
pure.
abundant
necessary
essential
under
ciliary
is
second
body,
constitutes
the
duction
the
human
humans.
hydrase the
Rodrigues,
is
vrich
in
141
14
I N
ZINC
E.
merit of
A"!D
3LOOD
AND
Fare,
Romao,
INHIBITION
140
13
A comparision of mitomycin C with 5-fluorouracil as an aqueous protein concentration adjunct to trabeculectomy: change K. Kawase. K. Nishimura. T . Yamamoto, S. Jikihara & Y. School of Kitazawa. Dept. of Gphthalmol., Gifu Univ. Medicine, Gifu-shi, Japan Possible mitomycin C (MMC) over 5superiority of fluorouracil (5-FU) as an adjunct to trabeculectomy has recently been reported by several authors. In order to compared their effects on the blood-aqueous barrier by measuring aqueous protein concentration in human Subjects trabeculectomy cases treated with either drug. were selected from a total body of 51 cases (65 eye*) treated with MMC and 15 cases’ (20 with 5-FU eyes) according to the following selection criteria to match type of glaucoma, postoperative anterior the two groups: chamber depth, age and other factors known to influence aqueous protein concentration. Thirteen eyes of 13 cases and 7 in MMC group (48.7 + 14.3 yrs, mean + SD) eyes of 7 cases in 5-FU group (58.0 + 13.1 yrs) were thus selected. In the MMC group, 0.2mg MMC was applied for 5 minutes intraoperatively. In 5-FU group, 5mg 5-FU W&¶ subconjunctivally injected repeatedly for the postoperative 2 weeks. We measured the aqueous protein concentration preoperatively and 1,2,3,5,7,14,28 days postoperatively with Laser Flare-Cell Meter@(FC-1000, Kowa, Japan). Mean aqueous protein concentration in the MMC group was significantly lower than that of the 5-FU group on the 2nd postoperative day (128.2 mg/dl vs 399.9; pco.05, Mann-Whitney U test). These results suggest that 5-FU has less favorable effects on BAB than MMC in early postoperative period.
IiUtiOR
but
humor.
142
15 AQUALITY
ASSEWOFXWDOnI
ZED CONTROL GLAUCoIII\
OF OPEW-A#GLE Orzalesi N. Rossetti L. Eye Clinic, University of Milan, S.Paolo Hospital, Milan, Italy TREATMKNT
TRIALS
ON
MEDICAL
SOURCE
OF AQUEOUS
Dept. of Ophthalmology, Institute
of
Biomedical
HUMOR
HYDROGEN
Univ. of Maryland,
PEROXIDE
Baltimore,
Maryland
USA
Sciences,
Hydrogen peroxide is one of the central member of the group of active oxygen dexivativc3. It has remluy IKea dcmalstreted tc be plemt in the normal lenses of most of the animal species ia amounts - lO+f. Aqueous humor of patients with some cataracts also have ekvated levels of peroxkkofthesimilarorder. Thesuurceoftheaqwusperox&ofthe cataract patialb, however, remeinl tmlesdval. Tltcsc llnwt@hs were U~touuunineifitcanbcderivedbyalePksse~rnthe eataraetous lenses. After 3, 6 & 11 days of feeding 35% gala&w diet, the animals were anesthetized with kekMneHC!L aad aqueous withdrawn for Hz& analysis. Subsequeotly, the animals were sacriftcul aad lenses isolated and extra&d with hichloroacetic acid (10%). H&J was estimated in the aqwous and lens extract be reacting them with I-“C-aketoglufarie acid and measuring C& formed by dec&oxylation. The levelSOfH*~inhecontrolaquausmdltnswae5f3and190~U) pM. InulcgaIaetmfcdanimrdsPfter11days,tlteaqucouslevcls incmaacdtoIbf4wdthe~s~~dccraasdb69f20IJII. These changestookpke-asafimedonoftimc. Treamwtofgalwosefed euimals with quereihiD (60 mgklayh) and smbieil (2 IQ&/day/rat) preventsdegsiastthe~ intheaqueowmdtbefaUintbelensH,~ contents. Thus, the attenuation of catamct pmva~ts against the A& leakage from the lens. The results indicate that the lens could be a significant swrce of aqueous peroxide.
A meta-analysis and a qualitative overview of published RCTs was undertaken to assess the effectiveness of medical treatment on outcome of patients with POAG. 102 RCTs were published between 1975 and 1991, totalling about 5000 patients. Only 16/102 were properly designed (i.e. an active treatment vs a placebo) to answer the questlnn. Pooling data from suitable studies (~6) a moderate yet statistically significant reduction in mean IOP was shown (-4.9 mmHg). Only 3 RCTs had, however, data on long-term visual field changes and their statistical combination failed to show a significant protective effect (O&0.75.95% C.I.=O.42-1.35) of active treatment. The methodology of the 102 RCTs was also reviewed. The analysis showed serious methodological drawbacks. In 37% RCTs no assessment of visual field abnormalities was made, in 34% RCTs more than 15% patients were lost to follow-up and 53% RCTd excluded them from the final analysis. In addition only 4% RCTs were planned following a priori estimate of sample size, 76% RCTs reported no information on treatment adherence and in 69% trials nt) information about the method of randomization was mentioned. Therefore the vast majority of published trials seems to be of limited value to guide clinical practice.
s.45