- Email: [email protected]
Physiology and genetics of candidate probiotic strains relevant to animal health and production
S50
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
time and the equipment cost. Both microorganisms were immobilized on delignified BSG. Electron microscope spectroscopy was used for the observation of cells immobilization. High conversion rate of BSG starch to bioethanol was achieved by this biological, low-cost and environmentally friendly method that could be applied in the utilization of starchy agro-industrial wastes.
play an important locus specific role in the initial stages of stem cell commitment to the cardiac lineage. Elucidating the molecular pathways involved in the gene cascade during the commitment of stem cells into the cardiac lineage is essential for developing techniques to generate large quantities of functional heart cells to be used in therapy.
doi:10.1016/j.copbio.2011.05.130
doi:10.1016/j.copbio.2011.05.132
OP60
OP62
NT-3 and IGFBP-2 increase the expansion of umbilical cord blood hematopoietic progenitors
Development of a dental biomaterials cloud computing concept
Betul Celebi 1 , Diego Mantovani 1 , Nicolas Pineault 2
Emilija Ilija Ristova 1 , Zoran Panov 2 , Vladan Borko Andonovik 1
1
1
Laboratory for Biomaterials and Bioengineering, Laval University, Canada 2 Hema-Quebec R&D Department, Quebec City, Canada E-mail address: [email protected] (B. Celebi) Limited numbers of hematopoietic progenitors in Cord Blood (CB) unit is a major limitation to their clinical use. Expansion of CB cells in co-cultures with mesenchymal stem cells (MSC) is an effective strategy to increase the cell dose available. We recently identified IGFBP-2 and NT-3 as two factors secreted by MSCs that might account for the pro-expansion activity of MSCs. Determine whether addition of IGFBP-2 and/or NT-3 to CB cultures can mimic the pro-expansion effects of MSCs. CB CD34+ cells were cultured in serum-free medium with or without NT-3 and/or IGFBP-2 for 6 days in presence of other cytokines favouring myeloid and MK progenitor expansions. Total cell expansion was significantly increased (1.3-fold, P = 0.01) when IGFBP2 and NT-3 were added to cultures. Myeloid progenitor (CFU-C) expansions were also increased by 1.3fold (89.5 ± 7.5 versus 67.2 ± 1.9, P = 0.05) and 1.5-fold (99.2 ± 23.7 versus 67.2 ± 1.9) in the presence of NT-3 and IGFBP-2 + NT-3. IGFBP-2 + NT-3 also increased the expansion of MK progenitors (1.3-fold), though this effect was not significant. These results support the concept that IGFBP-2 and NT-3 may mediate some of the pro-expansion activity of MSCs on hematopoietic progenitors doi:10.1016/j.copbio.2011.05.131
OP61 The role of histone acetyltransferases in cardiomyogenesis
Faculty of Mechanical Engineering-Vinica, Macedonia, University “Goce Delcev” Stip, Macedonia 2 Faculty of Natural and Technical Science-Stip, Macedonia, University “Goce Delcev” Stip, Macedonia E-mail address: [email protected] (E.I. Ristova) A developed digital library that includes dental biomaterials and materials for medical devices is a good topic for scientists to consider moving some data to the Clouds. This theoretical investigation describes possibilities of using the modern Cloud computing paradigm in conjunction with restorative dental biomaterials. In order to gather together the broad knowledge from this interdisciplinary field, it is necessary to have a strategy and road map for how and when to use cloud services within a company that deals with dental biomaterials. Masses of data resulting from experiments and other estimations are organized into more effective technologies for intelligent content creation and management. An effort has been made to close the knowledge gap between the innovators who design medical devices and the engineers who best understand materials and their capabilities. Small and Medium Business companies are increasingly realizing that simply by tapping into the Clouds they can gain fast access to the best business applications or drastically boost their infrastructure resources, all at negligible cost. Apart from the cost and time-sharing, the use of Clouds help companies share data and other research related information with other companies (partners, suppliers and competitors). doi:10.1016/j.copbio.2011.05.133
Poster Presentations
Ayse Elif Yilbas, Hymn Mach, Qiao Li Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada E-mail address: [email protected] (A.E. Yilbas) Cardiovascular diseases are among the leading causes of death worldwide. Currently, there are no effective treatment options for repairing the damaged heart tissue following cardiovascular incidents including myocardial infarctions. Therefore, a cell based therapy utilizing cardiomyocytes generated from stem cells will be a promising approach. However, the molecular mechanisms regulating stem cell differentiation into cardiomyocytes is not fully understood. Using chromatin immunoprecipitation, we have observed that p300 interacts with the enhancer and promoter regions of the transcription factor GATA-4 at a specific locus. Moreover, the histone acetyltransferase activity of p300 correlated with histone acetylation temporally during cardiomyogenesis in murine embryonic carcinoma (EC) and stem cells (ES). Thus p300 seems to
Animal Biotechnology Section A1 Physiology and genetics of candidate probiotic strains relevant to animal health and production Francesca Cecchini, Edo D’agaro, Marisa Manzano Department of Food Science, University of Udine, Via Sondrio 2/A, 33100 Udine, Italy E-mail address: [email protected] (F. Cecchini) Probiotic microorganism inclusion in feeds is emerging as a popular and potentially valuable tool in the practice of human and veterinary medicine and animal production. Recent research work has contributed to clarify the appropriate probiotic organisms, appropriate doses and conditions that are potentially treatable with probiotic strains in several farm animal species. The appealing
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
properties of probiotics include the ability to reduce antibiotic use, the apparently very high index of safety, and the public’s perception about ‘natural’ or ‘alternative’ therapies. Probiotic organisms must survive the acidic environment of the stomach and resist to the digestion process, adhere to the intestinal lining, colonize the intestinal tract and produce beneficial factors and inhibit pathogens (disease-causing bacteria). Other properties such as immunomodulation and modulation of metabolic activities are also desirable. Probiotics must be identified at the strain level from faecal and intestinal samples by means of molecular tools (DGGE, Real-time PCR analysis) and testing must be performed on individual strains. doi:10.1016/j.copbio.2011.05.134
A2 Mechanical test simulations using FEA of sheep tibia verified by the engineering data of the biomechanical tests Ibrahim Kutay Yilmazcoban 1 , Nursel Kiratli Yilmazcoban 2 , Ahmet Tasci 1 , Aykut Kahraman 1 , Isa Unal 1 1
Sakarya University, Mechanical Engineering Department, Sakarya, Turkey 2 Sakarya University, Environmental Engineering Department, Sakarya, Turkey E-mail address: [email protected] (A. Tasci) To understand the body behaviour and motion mechanics, primarily the skeleton system should be understood correctly. To determine the bone properties and a reference guide, basic mechanical tests are required. In this study, tibia bones were used for the basic mechanical tests. To get more reliable and usable data, many tests should be performed. To decrease the difficulty of the test conditions and time, and to obtain more results, CAD models of bones were simulated in Finite Element Analysis (FEA). For a simple approach, sheep tibias, similar to human humerus bones, were used for the tensile, compression and three point bending tests. To define a reliable biomechanical test in a computer simulation, mechanical properties are required. The properties of sheep tibia bones obtained from the tests were used to prepare the FEA models. Finally the tibia bones test results of forces and stresses were compared to computer simulation results and the differences shown. The study gives different test results as reference data and demonstrates that FEA modeling of bones are helpful and supportive for the human or animal biomechanics in real life conditions. doi:10.1016/j.copbio.2011.05.135
A3 Tensile tests and finite element analysis of different animal skins Ibrahim Kutay Yilmazcoban 1 , Nursel Kiratli Yilmazcoban 2 , Tuncay Avci 1 , Yasar Kahraman 1 , Sedat Iric 1 1
Mechanical Eng Dept, Engineering Faculty, Sakarya University, Sakarya, Turkey 2 Environmental Eng. Dept, Engineering Faculty, Sakarya University, Sakarya, Turkey E-mail address: [email protected] (I.K. Yilmazcoban) To determine the flexibility and mechanical properties of skins, basic tensile test of different animal skins were carried out. To get an enough skin properties and understand behavior of it, lots
S51
of tensile tests carried out. To see the stresses, strains and forces of skin while in various conditions in daily life with the help of test results, different animal skins’ tensile tests were calculated by Finite Element Analysis (FEA). For the skin tensile tests, skin sample of sheep, cows and chickens were used. To define a setup for real life conditions in computer simulations, mechanical properties are required. The properties of skins were received from the tensile tests. The FEA models were prepared up to the mechanical test results of the sheep, cows and chickens skin samples. The tensile test results and computer simulation results of different animal skin samples were compared by forces and stresses and the differences were shown. The results of this study give considerable correlation between the test results and the FEA modeling of skins. Simulation and test results are helpful and a reference guide for the human or the animal biomechanics in real life conditions in different activities. doi:10.1016/j.copbio.2011.05.136
A4 Different four PCR-multiplex systems via twenty microsatellite loci in goat Ozgecan Korkmaz Agaoglu 1 , Bengi Cinar Kul 2 , Bilal Akyuz 3 , Emel Ozkan 4 , Okan Ertugrul 2 1 Department of Animal Science, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey 2 Department of Genetic, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey 3 Department of Animal Science, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey 4 Department of Animal Science, Agricultural Faculty, Namik Kemal University, Tekirdag, Turkey
E-mail address: [email protected] (O.K. Agaoglu) Fluorescent based automated fragment analysis with multiplexing is a cost-effective, time-effective and labor-effective way to increase the throughput for simultaneously typing of numerous microsatellite markers. It is aimed to develop different multiplex systems via twenty microsatellites in goat for genetic characterization. PCR with the amplification of DNA used per reaction the amount of DNA, MgCl2 , primer and dNTPs concentrations and primers binding to each of temperatures (Ta) have been optimized. All microsatellites used in this study successfully amplified in four multiplex sets designed considering annealing temperature, product size and Beckman Coulter for spesific dye label. Multiplexing reduces the time, labor and cost of microsatellite typing. Multiplex PCR amplifications are technically more difficult than single-locus amplifications. However, optimization of the method used successfully established, multiplex-PCR system is more appropriate. The applicability of these systems was demonstrated for population structure and genetic diversity in goat. doi:10.1016/j.copbio.2011.05.137
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
time and the equipment cost. Both microorganisms were immobilized on delignified BSG. Electron microscope spectroscopy was used for the observation of cells immobilization. High conversion rate of BSG starch to bioethanol was achieved by this biological, low-cost and environmentally friendly method that could be applied in the utilization of starchy agro-industrial wastes.
play an important locus specific role in the initial stages of stem cell commitment to the cardiac lineage. Elucidating the molecular pathways involved in the gene cascade during the commitment of stem cells into the cardiac lineage is essential for developing techniques to generate large quantities of functional heart cells to be used in therapy.
doi:10.1016/j.copbio.2011.05.130
doi:10.1016/j.copbio.2011.05.132
OP60
OP62
NT-3 and IGFBP-2 increase the expansion of umbilical cord blood hematopoietic progenitors
Development of a dental biomaterials cloud computing concept
Betul Celebi 1 , Diego Mantovani 1 , Nicolas Pineault 2
Emilija Ilija Ristova 1 , Zoran Panov 2 , Vladan Borko Andonovik 1
1
1
Laboratory for Biomaterials and Bioengineering, Laval University, Canada 2 Hema-Quebec R&D Department, Quebec City, Canada E-mail address: [email protected] (B. Celebi) Limited numbers of hematopoietic progenitors in Cord Blood (CB) unit is a major limitation to their clinical use. Expansion of CB cells in co-cultures with mesenchymal stem cells (MSC) is an effective strategy to increase the cell dose available. We recently identified IGFBP-2 and NT-3 as two factors secreted by MSCs that might account for the pro-expansion activity of MSCs. Determine whether addition of IGFBP-2 and/or NT-3 to CB cultures can mimic the pro-expansion effects of MSCs. CB CD34+ cells were cultured in serum-free medium with or without NT-3 and/or IGFBP-2 for 6 days in presence of other cytokines favouring myeloid and MK progenitor expansions. Total cell expansion was significantly increased (1.3-fold, P = 0.01) when IGFBP2 and NT-3 were added to cultures. Myeloid progenitor (CFU-C) expansions were also increased by 1.3fold (89.5 ± 7.5 versus 67.2 ± 1.9, P = 0.05) and 1.5-fold (99.2 ± 23.7 versus 67.2 ± 1.9) in the presence of NT-3 and IGFBP-2 + NT-3. IGFBP-2 + NT-3 also increased the expansion of MK progenitors (1.3-fold), though this effect was not significant. These results support the concept that IGFBP-2 and NT-3 may mediate some of the pro-expansion activity of MSCs on hematopoietic progenitors doi:10.1016/j.copbio.2011.05.131
OP61 The role of histone acetyltransferases in cardiomyogenesis
Faculty of Mechanical Engineering-Vinica, Macedonia, University “Goce Delcev” Stip, Macedonia 2 Faculty of Natural and Technical Science-Stip, Macedonia, University “Goce Delcev” Stip, Macedonia E-mail address: [email protected] (E.I. Ristova) A developed digital library that includes dental biomaterials and materials for medical devices is a good topic for scientists to consider moving some data to the Clouds. This theoretical investigation describes possibilities of using the modern Cloud computing paradigm in conjunction with restorative dental biomaterials. In order to gather together the broad knowledge from this interdisciplinary field, it is necessary to have a strategy and road map for how and when to use cloud services within a company that deals with dental biomaterials. Masses of data resulting from experiments and other estimations are organized into more effective technologies for intelligent content creation and management. An effort has been made to close the knowledge gap between the innovators who design medical devices and the engineers who best understand materials and their capabilities. Small and Medium Business companies are increasingly realizing that simply by tapping into the Clouds they can gain fast access to the best business applications or drastically boost their infrastructure resources, all at negligible cost. Apart from the cost and time-sharing, the use of Clouds help companies share data and other research related information with other companies (partners, suppliers and competitors). doi:10.1016/j.copbio.2011.05.133
Poster Presentations
Ayse Elif Yilbas, Hymn Mach, Qiao Li Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada E-mail address: [email protected] (A.E. Yilbas) Cardiovascular diseases are among the leading causes of death worldwide. Currently, there are no effective treatment options for repairing the damaged heart tissue following cardiovascular incidents including myocardial infarctions. Therefore, a cell based therapy utilizing cardiomyocytes generated from stem cells will be a promising approach. However, the molecular mechanisms regulating stem cell differentiation into cardiomyocytes is not fully understood. Using chromatin immunoprecipitation, we have observed that p300 interacts with the enhancer and promoter regions of the transcription factor GATA-4 at a specific locus. Moreover, the histone acetyltransferase activity of p300 correlated with histone acetylation temporally during cardiomyogenesis in murine embryonic carcinoma (EC) and stem cells (ES). Thus p300 seems to
Animal Biotechnology Section A1 Physiology and genetics of candidate probiotic strains relevant to animal health and production Francesca Cecchini, Edo D’agaro, Marisa Manzano Department of Food Science, University of Udine, Via Sondrio 2/A, 33100 Udine, Italy E-mail address: [email protected] (F. Cecchini) Probiotic microorganism inclusion in feeds is emerging as a popular and potentially valuable tool in the practice of human and veterinary medicine and animal production. Recent research work has contributed to clarify the appropriate probiotic organisms, appropriate doses and conditions that are potentially treatable with probiotic strains in several farm animal species. The appealing
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
properties of probiotics include the ability to reduce antibiotic use, the apparently very high index of safety, and the public’s perception about ‘natural’ or ‘alternative’ therapies. Probiotic organisms must survive the acidic environment of the stomach and resist to the digestion process, adhere to the intestinal lining, colonize the intestinal tract and produce beneficial factors and inhibit pathogens (disease-causing bacteria). Other properties such as immunomodulation and modulation of metabolic activities are also desirable. Probiotics must be identified at the strain level from faecal and intestinal samples by means of molecular tools (DGGE, Real-time PCR analysis) and testing must be performed on individual strains. doi:10.1016/j.copbio.2011.05.134
A2 Mechanical test simulations using FEA of sheep tibia verified by the engineering data of the biomechanical tests Ibrahim Kutay Yilmazcoban 1 , Nursel Kiratli Yilmazcoban 2 , Ahmet Tasci 1 , Aykut Kahraman 1 , Isa Unal 1 1
Sakarya University, Mechanical Engineering Department, Sakarya, Turkey 2 Sakarya University, Environmental Engineering Department, Sakarya, Turkey E-mail address: [email protected] (A. Tasci) To understand the body behaviour and motion mechanics, primarily the skeleton system should be understood correctly. To determine the bone properties and a reference guide, basic mechanical tests are required. In this study, tibia bones were used for the basic mechanical tests. To get more reliable and usable data, many tests should be performed. To decrease the difficulty of the test conditions and time, and to obtain more results, CAD models of bones were simulated in Finite Element Analysis (FEA). For a simple approach, sheep tibias, similar to human humerus bones, were used for the tensile, compression and three point bending tests. To define a reliable biomechanical test in a computer simulation, mechanical properties are required. The properties of sheep tibia bones obtained from the tests were used to prepare the FEA models. Finally the tibia bones test results of forces and stresses were compared to computer simulation results and the differences shown. The study gives different test results as reference data and demonstrates that FEA modeling of bones are helpful and supportive for the human or animal biomechanics in real life conditions. doi:10.1016/j.copbio.2011.05.135
A3 Tensile tests and finite element analysis of different animal skins Ibrahim Kutay Yilmazcoban 1 , Nursel Kiratli Yilmazcoban 2 , Tuncay Avci 1 , Yasar Kahraman 1 , Sedat Iric 1 1
Mechanical Eng Dept, Engineering Faculty, Sakarya University, Sakarya, Turkey 2 Environmental Eng. Dept, Engineering Faculty, Sakarya University, Sakarya, Turkey E-mail address: [email protected] (I.K. Yilmazcoban) To determine the flexibility and mechanical properties of skins, basic tensile test of different animal skins were carried out. To get an enough skin properties and understand behavior of it, lots
S51
of tensile tests carried out. To see the stresses, strains and forces of skin while in various conditions in daily life with the help of test results, different animal skins’ tensile tests were calculated by Finite Element Analysis (FEA). For the skin tensile tests, skin sample of sheep, cows and chickens were used. To define a setup for real life conditions in computer simulations, mechanical properties are required. The properties of skins were received from the tensile tests. The FEA models were prepared up to the mechanical test results of the sheep, cows and chickens skin samples. The tensile test results and computer simulation results of different animal skin samples were compared by forces and stresses and the differences were shown. The results of this study give considerable correlation between the test results and the FEA modeling of skins. Simulation and test results are helpful and a reference guide for the human or the animal biomechanics in real life conditions in different activities. doi:10.1016/j.copbio.2011.05.136
A4 Different four PCR-multiplex systems via twenty microsatellite loci in goat Ozgecan Korkmaz Agaoglu 1 , Bengi Cinar Kul 2 , Bilal Akyuz 3 , Emel Ozkan 4 , Okan Ertugrul 2 1 Department of Animal Science, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey 2 Department of Genetic, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey 3 Department of Animal Science, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey 4 Department of Animal Science, Agricultural Faculty, Namik Kemal University, Tekirdag, Turkey
E-mail address: [email protected] (O.K. Agaoglu) Fluorescent based automated fragment analysis with multiplexing is a cost-effective, time-effective and labor-effective way to increase the throughput for simultaneously typing of numerous microsatellite markers. It is aimed to develop different multiplex systems via twenty microsatellites in goat for genetic characterization. PCR with the amplification of DNA used per reaction the amount of DNA, MgCl2 , primer and dNTPs concentrations and primers binding to each of temperatures (Ta) have been optimized. All microsatellites used in this study successfully amplified in four multiplex sets designed considering annealing temperature, product size and Beckman Coulter for spesific dye label. Multiplexing reduces the time, labor and cost of microsatellite typing. Multiplex PCR amplifications are technically more difficult than single-locus amplifications. However, optimization of the method used successfully established, multiplex-PCR system is more appropriate. The applicability of these systems was demonstrated for population structure and genetic diversity in goat. doi:10.1016/j.copbio.2011.05.137