Plasma deoxycholic acid is related to deoxycholic acid in faecal water

CANCER LETTERS

EISEVIER

Cancer

Letters

114 (1997)

293-294

Plasma deoxycholic acid is related to deoxycholic acid in faecal water Arendina van Faassenay*, Thomas Ochsenkiihnb, Saskia Houtermana, Elisabetb M.C. van der PloegC, Bas H. Bueno-de-Mesquitad, Marga C. Ockdd, Ekkehard Bayerd6rfferb, Ruud A. Janknegt” bDepartment ‘Depanment

‘Department of Urology, University Hospital, P.O. Box 5800, 6202 AZ Maastricht, The Netherlands of Internal Medicine II, Klinikum Grosshadem, University of Miinchen, Marchioninistrasse 15, 81377 Miinchen, Germany ‘Department of Dietetics, University Hospital, Maastricht, The Netherlands of Chronic Diseases and Environmental Epidemiology, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands

Abstract Bile acids are considered as a risk factor for colorectal carcinogenesis. They were analysed in samples of faecal water and plasma of fasting heparine blood from 23 urolithiasis patients. Linear regression showed that the highest percentage of variance (52%) was explained by the model: plasma deoxycholic acid (pmol/l) = -3.11 + 0.96(&0.25*) ?og deoxycholic acid in faecal water (PmolA) + 0.35(M.15*) pH of faecal water - [email protected]*) defacation frequency (number of stools/day); *P < 0.05, “P = 0.055. In future studies, analysing blood levels of unconjugated deoxycholic acid may substitute faecal measurements. 0 1997 Elsevier Science Ireland Ltd. Keywords: Bile acids; Colorectal cancer; Diet

1. Introduction

Unconjugated deoxycholic acid (DC) and isodeoxycholic acid in serum were found to be higher in patients with colorectal adenoma than in controls [ 11. Also, faecal bile acids were increased in patients with colorectal adenoma [2] and the rate of neoplastic polyp recurrence in the colon increased with the faecal concentration of bile acids [3]. DC in faecal water was lower when the intake of saturated fat was lower and the intake of dietary fibre from grains and from

*Corresponding 3614349.

author.

Tel.:

+31 43 3881237,

0304-3835/97/$17.00 0 1997 Elsevier PZZ SO304-3835(97)04683-l

fax:

+31 43

fruits and vegetables was higher [4]. The hypothesis of our study was that there is a correlation between the concentration of unconjugated bile acids in fasting plasma and those in faecal water. If the correlations are high enough, analysing plasma may substitute faecal measurements of bile acids.

2. Materials

and methods

We analysed unconjugated bile acids (BA) in baseline samples of faecal water and plasma of fasting heparine blood from 23 patients who were prescribed a calcium-restricted diet for urolithiasis due to hypercalciuria type II.

Faecal water was obtained by high speed centrifu-

Science

Ireland

Ltd. All rights reserved

gation of faeces and the BA by diethylether extraction of faecal water [4]. Bile acids from plasma were obtained by column chromatography after solvolysis of plasma. The unconjugated BA were analysed bv capillary gas-liquid chromatography /I ].

individual variability of DC in fasting and non-tasting blood will be measured, In conclusion. the concentration of unconjugated DC and ursodeoxycholic acid in fasting ptasma ia related to their respective faecal water concentrations. In future studiesanalysing blood DC levels may sub stitute faecal measurements.

3. Results Linear regression showed that the highest percentage of variance (52%) was explained by the model: plasma DC (pmol/l) = - 3.11 + 0.96+0.25*) ‘“log DC in faecat water (pmol/lj + 0.35(+0.15*) pH of faecal water - 0.41(&O. 19#j defacation frequency (number of stools/dayj; *P < 0.05, 'P = 0.055, For plasma ursodeoxycholic acid 27% of the variance was explained by 0.00 + 0.13(tij.O5)* “‘log ursodeoxycholic acid in faecal water: *P
Acknowledgements The study was financiahy supported by the European Commission in the Europe Against Cancer program.

Referewes I&iycrdiirl’fcr. I’.. Wi~+cche, vxondary

4. Discussion The amount of DC in faecal water contributes the most to the variance in plasma DC. A high defacation frequency mostly accompanies a low transit time and therefore there is less time to absorb BA in the colon. A low pH of faecal water may be a marker of a colonic environment that decreases the absorption of DC. pas-sibly due to increased adsorption of bile acids to hacterial debris and fibre. Further research wit1 focus on the relation between DC in plasma and intake of dietary fibre. Also. intra-

aden0miis

Imray. C.W.,

I,.. .Manne~, G.:\., K and Paumgartner. bile

in the serum

J.t’. with

( 1992)

colorectal

unconjugati or polyps,

McKeown-Fyssen, G., Bright-See. V. and the Toronto Polyp Prevention mized trial of s low fat high fibre colorcctal

polyps.

.I. Clin.

with

colorectal

A.. Barker. G.. Hendricksc. A.M., Baker. P.R. and

Faecal cancer

I ., I)uschedl. linconjugatcd

of patients

. . (ilIt ~ Xi. XX-271 C.H.E Kadley. S.. Davis. Donovan. I-4.. Lawson,

Neoptolemos. patients 1245.

xids

Ochaenkiihn, Cr. (1995)

Epidemiol..

biic Gut.

acids 33.

in

1239

E.. Bruce. W.R., Jazmajt. Grvq f 1994) A randodiet in the. recurrence of 47, 5?S

56.

van Lassen, A., Hazen, M.J., van den Hrandt. P.A., v%11 den Bogaard.. /\.I:.. Hermus. R.J.J. and Janknegt, R.A. (1993) Bile acids and pH values in total faeces and in faecal water from hahituatly ommvorous Nun,. 5X. 917. 031

and

vegetarian

subjects.

Am

.I. Clin.