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Plasma levels of enteroglucagon in patients affected by cystic fibrosis
P.51 PLASMA LEVELS OF ENTEROGLUCAGON IN PATIENTS AFFECTED BY CYSTIC FIBROSIS. M.Castro, V.Lucidi, M.Gambarara, F.Ferretti, P.Mariani, B.Papadatou, A.M.Colombo, S.Bella, R.Giampaolo. -----__-Division of Pediatric Gastroenterology, Ospedale Bambino Gesti, Roma, Italia.
Enteroglucagon (EG) is a gastrointestinal hormone which has a trophic effect on intestinal mucosa. Its production in the distal small intestine could be stimulated by the arrival of indigested food. We have studied the behaviour of plasmatic EG in patients affected by cystic fibrosis (C.F.), a pathology in which maldigestion is present. The study includes 21 children divided in 2 groups: group A including 13 children (9 males, 4 females), mean age: 6y 4m (range 1-12~1, affected by C.F., treated with pancreatic enzymes; group B including 8 children, same mean age, not affected by gastrointestinal diseases. Blood sample for EG dosage has been collected during fasting and has been treated with EDTA; separated plasma has been stored at -20 C . EG level has been determinated by radioimmunoassay with specific antibodies. Results are reported as following and they are expressed as a mean value T standard deviation; Student's two-tailed t test has been used to test differences between means. Group A: EG= 199 ? 42 pmol/l; group B: EG= 71 f 26 pmol/l, p 0,05. Our data show a statistically significant difference for fasting EG levels in the two groups. This difference is even more significant if we consider that our C.F. patients were already in substitutive opotherapy, probably insufficent to correct completely the enzyme's deficiency. Our results show the utility of fasting EG levels in patients affected by C.F. with a severe maldigestion; in particular, a high value of plasmatic EG could demostrate an incomplete correction of enzymatic defect.
p.52
WC"-UP
CRWT"
INFANTS.
MD
ba related to the nutritimnl
energy llrllutritim (PEW) on body ccqositim, Anthropmetric
-w-ts
OF EXTREIIELYMARASRIC
rstio(U/ll)~BD%,4492$36 grw. wre
state.
we studied the effect of the severity of protein
growth velocity md
energy atabolirn
at the urly
were rssesred at the start, w-d during the first 2 mmths
They were divided in two graqs
caloricinten
FUTURES
Digestive et Assistance Nutritive,
Wslades Paris.
catch-rq growth pattern my
wwp
unite de Remimtim
0. IKNLET, J. SILAS, C. RICWR.
Hopitat des Enfmts
therw.
PAMDOKIUL
ENERGY llETABoLtSNOLITOTAL PARENTERAL NUTRITIOY UPN):
A,,
wcording
to PRM dqrcc.
Group II n-14,
respxtiwly
Grap
5.7~3mnths
504&54ma/kg/d
md
I ~14,
U/N-,
5.e2.9
phase of Of TM
rutritial
in 26 infmts.
months, wciW/heipht
4012_~ sra. DailynitrO#mandnonprotein
405.2?72KJ/kg/d in Crap
md I, SD4__+6(kq/kgld
309.4+54KJ/kg/d in
II.
m
after 60 days of nutritional support, the U/N, Fat Body Rass/ucight(FSWU)
before md
and Lam
Body IWWheight
(LBIIIN)aI-*shoun.
G-1 Ln e&g+3
w/so FEWM
6.8 + 3
(x>
=
uV*m In tarp
+ 2.3
mwgy
ra@rcmt
283KJ/kg/d. The mgy weight prin yu ralrirr*nt YS
90.626
7D
12.2 2 3.2
3.4 = 3.3
96.9 + 1.7
B-c*_ 5.3
*?
87:
11
9.7 + 4.4 W-6
+ 3.4 gain w
922~176#/6D dsys cceposd
of 42.211fat.
The
calculated fran single regression analysis of wright gain against energy intake wa
cost of the wcibt
1~_305g/MJd
md
us
gain Y*
39.4KJ/g.
In Graq
II, height increase was 2.7$1.3cWDd
conposed of 27 X fat. Its energy cost UB
28.7KJlg. The maintmmcc
md
energy
zzoIJ/kg/d. these results indlcatc that in
but sIso of LRWI
2.) In the catch-q
accuwletes. 3.) lhe amroy is related to the rutritiml rnaintme
GluxP II dM
I, height increase wos 4.01+1.7m/60 c*yr and might
naintmmcc
~alujpp:
8
m
lxtrmely
nrresmic infmts on TPN 1.) there is not only a decrease of Fw
grcuth, there is m
cost of mplcting
initisl preferential it-creaseof LRM nd
state. 5.) tn I aban severe state of nslrutrition less calories WC
of wai(lht.6.1 At my
gfvm
less fat
tissue is Lcuer. 4.1 Thus, the rate at which a depleted LRM is restored
caloric intake &eve
greater in the more severely malnourished infant.
that wired
for mintcnace,
required for
the restoration rate is
Enteroglucagon (EG) is a gastrointestinal hormone which has a trophic effect on intestinal mucosa. Its production in the distal small intestine could be stimulated by the arrival of indigested food. We have studied the behaviour of plasmatic EG in patients affected by cystic fibrosis (C.F.), a pathology in which maldigestion is present. The study includes 21 children divided in 2 groups: group A including 13 children (9 males, 4 females), mean age: 6y 4m (range 1-12~1, affected by C.F., treated with pancreatic enzymes; group B including 8 children, same mean age, not affected by gastrointestinal diseases. Blood sample for EG dosage has been collected during fasting and has been treated with EDTA; separated plasma has been stored at -20 C . EG level has been determinated by radioimmunoassay with specific antibodies. Results are reported as following and they are expressed as a mean value T standard deviation; Student's two-tailed t test has been used to test differences between means. Group A: EG= 199 ? 42 pmol/l; group B: EG= 71 f 26 pmol/l, p 0,05. Our data show a statistically significant difference for fasting EG levels in the two groups. This difference is even more significant if we consider that our C.F. patients were already in substitutive opotherapy, probably insufficent to correct completely the enzyme's deficiency. Our results show the utility of fasting EG levels in patients affected by C.F. with a severe maldigestion; in particular, a high value of plasmatic EG could demostrate an incomplete correction of enzymatic defect.
p.52
WC"-UP
CRWT"
INFANTS.
MD
ba related to the nutritimnl
energy llrllutritim (PEW) on body ccqositim, Anthropmetric
-w-ts
OF EXTREIIELYMARASRIC
rstio(U/ll)~BD%,4492$36 grw. wre
state.
we studied the effect of the severity of protein
growth velocity md
energy atabolirn
at the urly
were rssesred at the start, w-d during the first 2 mmths
They were divided in two graqs
caloricinten
FUTURES
Digestive et Assistance Nutritive,
Wslades Paris.
catch-rq growth pattern my
wwp
unite de Remimtim
0. IKNLET, J. SILAS, C. RICWR.
Hopitat des Enfmts
therw.
PAMDOKIUL
ENERGY llETABoLtSNOLITOTAL PARENTERAL NUTRITIOY UPN):
A,,
wcording
to PRM dqrcc.
Group II n-14,
respxtiwly
Grap
5.7~3mnths
504&54ma/kg/d
md
I ~14,
U/N-,
5.e2.9
phase of Of TM
rutritial
in 26 infmts.
months, wciW/heipht
4012_~ sra. DailynitrO#mandnonprotein
405.2?72KJ/kg/d in Crap
md I, SD4__+6(kq/kgld
309.4+54KJ/kg/d in
II.
m
after 60 days of nutritional support, the U/N, Fat Body Rass/ucight(FSWU)
before md
and Lam
Body IWWheight
(LBIIIN)aI-*shoun.
G-1 Ln e&g+3
w/so FEWM
6.8 + 3
(x>
=
uV*m In tarp
+ 2.3
mwgy
ra@rcmt
283KJ/kg/d. The mgy weight prin yu ralrirr*nt YS
90.626
7D
12.2 2 3.2
3.4 = 3.3
96.9 + 1.7
B-c*_ 5.3
*?
87:
11
9.7 + 4.4 W-6
+ 3.4 gain w
922~176#/6D dsys cceposd
of 42.211fat.
The
calculated fran single regression analysis of wright gain against energy intake wa
cost of the wcibt
1~_305g/MJd
md
us
gain Y*
39.4KJ/g.
In Graq
II, height increase was 2.7$1.3cWDd
conposed of 27 X fat. Its energy cost UB
28.7KJlg. The maintmmcc
md
energy
zzoIJ/kg/d. these results indlcatc that in
but sIso of LRWI
2.) In the catch-q
accuwletes. 3.) lhe amroy is related to the rutritiml rnaintme
GluxP II dM
I, height increase wos 4.01+1.7m/60 c*yr and might
naintmmcc
~alujpp:
8
m
lxtrmely
nrresmic infmts on TPN 1.) there is not only a decrease of Fw
grcuth, there is m
cost of mplcting
initisl preferential it-creaseof LRM nd
state. 5.) tn I aban severe state of nslrutrition less calories WC
of wai(lht.6.1 At my
gfvm
less fat
tissue is Lcuer. 4.1 Thus, the rate at which a depleted LRM is restored
caloric intake &eve
greater in the more severely malnourished infant.
that wired
for mintcnace,
required for
the restoration rate is