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Plerocercoids of Spirometra erinaceieuropaei reduse gene expression of tumor necrosis factor-α in mice.
Poster Sessions I Parasitology Intehaational47 (Suppl.) (1998) 283-389
364
P-0907
VACCIl@ FOi? THE l?ROl?HYIAXISOF SfIEsP
BCHINCCOCCOSIS
CD80 AND CD86 ON IMMUNE RESPONSES M HYHENO4EEIs m IN MICE.
IN UZEEKISTAN
Aminghanov Y. Uzbek scientific-research institute of veterinary science, helminthozoonotic laboratory, Tailyak, Gsmarkand region, Uzbekistan. Schinococcosis belongs to the number of wide-spread helminthosis in Uzbekistan and next countries to it. The average rate of Echinococcosis defeat in sheep is 30-6C%. The annual losses from this disease are more than 5 milliards sum in average. In this connection we tested the vaccine against Achinococcosis on 55 current-year-born lambs in the laboratory conditions. The animals were expoeed to slaughter in 6,9,12 months after the infection. The results of the slaughter showed,that 6 from II lambs of the 1st experimental group, which get ths vaccine in the dose of I ml,were not infected and it consists 54,s. During the autopsy of the rest 5 heads 41 bladders from 72 discovered were dead. From the I,5 ml dose of vaccine the effectiVf?neSS was 63,795. Yore than 7c% of bladders were dead.
P-0908
p-0909
pLERocEBcoED8 OF SPmm ERIMCEIEUROPAEI BEDUSE GENE EXPRESSION OF TUMOR NECROSIS FACTOR -o. IN MICE.
s Tanihata T., Fukumoto S., Wang H., Hirai K. Department of Madical zoology, Faculty of Medicine, Tottori University, Yonagu, Japan Tumor necroeie factor m-a called proinflammatory cytokine is the pleiotmpic cytokioe that exhibits potent immunologic and inflammatory pmpertiee both in vitm and in viva. To investigate e&cta of ~piirometro erinaceiewopei plemcercoid infection, gene expreeeion of TNF-a wae evaluated on the spleen and peritoneal macmphagee in mice by Northern blot analyeie. We firat examined effects of co-culture with plerocercoide on TNF-a mRNA expression6 in cultured peritoneal macrophagee of mice. This gene expression in lipopolyeaccharide(S) activated macmphagee wae euppreeeed by co-cultivation with plerocercoide. In the second series of experiments, we examined effects of excretorylsecretory (EZ) products of plerocercoids on peritoneal macmphagee. ES products suppressed TNF-a gene macmphages. In the third series, expreeeion in LPS-activated we carried out to determine whether or not plemrcoide auppreee TNF-a mRNA expreeeion in viva. Thioglycollateinduced peritoneal macrophagoe were obtained from mice infected with plerocercoida and were stimulated with LPS in vitro. These macmphagee showed lower expression of TNF-a mRNA than tboae of uninfected control mice. Finally, mice infectedwith plemcercoidswere intravenously injected with LPS. The TNF-a gene exproeeion in spleen were reduced rather
thanthatof non-infected mice. Our findings suggest that plerocercoidssuppress host immuneresponseby reductionof TNF-ageneexpression.
Asano K", WaLtenabe N**, Hisamitsu T*, Ohtomo H**
*Department University,
of Physiology,
School
of Medicine,
Showa
Shinagawa-ku and **Department of Tropical Medicine, Jikei University School of Medicine, Minato-ku. Tokyo, Japan
It has been shown that Hvmenoleuis eggs induce strong protection through DTH in mice. Interaction between CD28 on T cells and CD80 and CDt36 on APC is one of costimulatory signals for optimal activation of T cells. Effect of CD80 and CD86 on protection end DTH were examined in H. infected mice. nice were immunized with 1) single oral administration of 1. nqIL eggs. When mice were challenged with eggs, complete protection we8 observed. Treatment of anti-CD80 and/or anti-CD86 could not affectcaais.~pwbective.reaponee. Horeover, complete protec'cion..wasalso found in similar experiment in CD28 KO mice. DTH reaction with egg-Ag in immunized mice, determined by footpad swelling, wss partially suppressed by the treatment with anti-CDSC. IFN-Y and IL-2 production by mesenteric lymph node cells from immunized mice, stimulated with egg-Ag &) yj&~, wes suppressed by the treatment with anti-CDBC. 1.l11 .I .I No effect of anti-CD80 was found on DTH end cytokine production. These results indicate that protective immunity is induced without CD80 and CD86, but DTH reaction depends on CD86, suggesting segregation on mechanisms of proCection end DTH.
P-0910
PREFW-IIAL PRODUCllON OF DlFFERENT CYTOKINES BY MACROPHAGES STlMlJlATED Wll’H MCREl-ORY/SlXREIORY (Es) AND BODY MTRACT ANTlGENS OF ASCARIS SCJUM LARVAE
I(uroda 4 Yamasbita U wt of Immunology. University of Occupational and Environmental Health, Kitekyushu, Japan. Nematode infection generally activates Th2 response in the infected hosts and induces the increased level of serum 1gE and eosinophilia. However, the precise mechanism for Th2 activation in the nematode-infected host is not fully understood. In the early stage of infection, macxophags are initially activated and produce several effecter moleculesto initiate the immune responses. In this communication, we investigated the effect of ES and body antigens of Ascoris suum larvae on murine macrophages in vitro. Thioglycolate-induccd peritoneal macrophages were stimulated with several antigens for 18 hr and cytokines released in the culture supernatant was measured with ELISA. When compared with LPS or SAC as a contmlstimulation, ES antigen prepared from the culture supematant of Ascti suum larvae stimulated mecrophagee to produce higher amount of IL-la and lower amount of IL12p40. On tbe other hand, body antigen stimulated macrophages to produce lower amount of 1Lla and comparable amount of IL-12p40. IL-6 and TNF were only marginally produced by both Es and body antigens stimulation. These results suggest that Es and body antigens activate macrophages to preferentially induce different cytokines which may result in the preferential activation of Thl and Tb2 subsets.
364
P-0907
VACCIl@ FOi? THE l?ROl?HYIAXISOF SfIEsP
BCHINCCOCCOSIS
CD80 AND CD86 ON IMMUNE RESPONSES M HYHENO4EEIs m IN MICE.
IN UZEEKISTAN
Aminghanov Y. Uzbek scientific-research institute of veterinary science, helminthozoonotic laboratory, Tailyak, Gsmarkand region, Uzbekistan. Schinococcosis belongs to the number of wide-spread helminthosis in Uzbekistan and next countries to it. The average rate of Echinococcosis defeat in sheep is 30-6C%. The annual losses from this disease are more than 5 milliards sum in average. In this connection we tested the vaccine against Achinococcosis on 55 current-year-born lambs in the laboratory conditions. The animals were expoeed to slaughter in 6,9,12 months after the infection. The results of the slaughter showed,that 6 from II lambs of the 1st experimental group, which get ths vaccine in the dose of I ml,were not infected and it consists 54,s. During the autopsy of the rest 5 heads 41 bladders from 72 discovered were dead. From the I,5 ml dose of vaccine the effectiVf?neSS was 63,795. Yore than 7c% of bladders were dead.
P-0908
p-0909
pLERocEBcoED8 OF SPmm ERIMCEIEUROPAEI BEDUSE GENE EXPRESSION OF TUMOR NECROSIS FACTOR -o. IN MICE.
s Tanihata T., Fukumoto S., Wang H., Hirai K. Department of Madical zoology, Faculty of Medicine, Tottori University, Yonagu, Japan Tumor necroeie factor m-a called proinflammatory cytokine is the pleiotmpic cytokioe that exhibits potent immunologic and inflammatory pmpertiee both in vitm and in viva. To investigate e&cta of ~piirometro erinaceiewopei plemcercoid infection, gene expreeeion of TNF-a wae evaluated on the spleen and peritoneal macmphagee in mice by Northern blot analyeie. We firat examined effects of co-culture with plerocercoide on TNF-a mRNA expression6 in cultured peritoneal macrophagee of mice. This gene expression in lipopolyeaccharide(S) activated macmphagee wae euppreeeed by co-cultivation with plerocercoide. In the second series of experiments, we examined effects of excretorylsecretory (EZ) products of plerocercoids on peritoneal macmphagee. ES products suppressed TNF-a gene macmphages. In the third series, expreeeion in LPS-activated we carried out to determine whether or not plemrcoide auppreee TNF-a mRNA expreeeion in viva. Thioglycollateinduced peritoneal macrophagoe were obtained from mice infected with plerocercoida and were stimulated with LPS in vitro. These macmphagee showed lower expression of TNF-a mRNA than tboae of uninfected control mice. Finally, mice infectedwith plemcercoidswere intravenously injected with LPS. The TNF-a gene exproeeion in spleen were reduced rather
thanthatof non-infected mice. Our findings suggest that plerocercoidssuppress host immuneresponseby reductionof TNF-ageneexpression.
Asano K", WaLtenabe N**, Hisamitsu T*, Ohtomo H**
*Department University,
of Physiology,
School
of Medicine,
Showa
Shinagawa-ku and **Department of Tropical Medicine, Jikei University School of Medicine, Minato-ku. Tokyo, Japan
It has been shown that Hvmenoleuis eggs induce strong protection through DTH in mice. Interaction between CD28 on T cells and CD80 and CDt36 on APC is one of costimulatory signals for optimal activation of T cells. Effect of CD80 and CD86 on protection end DTH were examined in H. infected mice. nice were immunized with 1) single oral administration of 1. nqIL eggs. When mice were challenged with eggs, complete protection we8 observed. Treatment of anti-CD80 and/or anti-CD86 could not affectcaais.~pwbective.reaponee. Horeover, complete protec'cion..wasalso found in similar experiment in CD28 KO mice. DTH reaction with egg-Ag in immunized mice, determined by footpad swelling, wss partially suppressed by the treatment with anti-CDSC. IFN-Y and IL-2 production by mesenteric lymph node cells from immunized mice, stimulated with egg-Ag &) yj&~, wes suppressed by the treatment with anti-CDBC. 1.l11 .I .I No effect of anti-CD80 was found on DTH end cytokine production. These results indicate that protective immunity is induced without CD80 and CD86, but DTH reaction depends on CD86, suggesting segregation on mechanisms of proCection end DTH.
P-0910
PREFW-IIAL PRODUCllON OF DlFFERENT CYTOKINES BY MACROPHAGES STlMlJlATED Wll’H MCREl-ORY/SlXREIORY (Es) AND BODY MTRACT ANTlGENS OF ASCARIS SCJUM LARVAE
I(uroda 4 Yamasbita U wt of Immunology. University of Occupational and Environmental Health, Kitekyushu, Japan. Nematode infection generally activates Th2 response in the infected hosts and induces the increased level of serum 1gE and eosinophilia. However, the precise mechanism for Th2 activation in the nematode-infected host is not fully understood. In the early stage of infection, macxophags are initially activated and produce several effecter moleculesto initiate the immune responses. In this communication, we investigated the effect of ES and body antigens of Ascoris suum larvae on murine macrophages in vitro. Thioglycolate-induccd peritoneal macrophages were stimulated with several antigens for 18 hr and cytokines released in the culture supernatant was measured with ELISA. When compared with LPS or SAC as a contmlstimulation, ES antigen prepared from the culture supematant of Ascti suum larvae stimulated mecrophagee to produce higher amount of IL-la and lower amount of IL12p40. On tbe other hand, body antigen stimulated macrophages to produce lower amount of 1Lla and comparable amount of IL-12p40. IL-6 and TNF were only marginally produced by both Es and body antigens stimulation. These results suggest that Es and body antigens activate macrophages to preferentially induce different cytokines which may result in the preferential activation of Thl and Tb2 subsets.