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SMALL ARTERIES AND ENDOTHELIUM-DEPENDENT AND NON-ENDOTHELIUM DEPENDENT VASODILATION
110
Poster Sessions PO14 Arterial structure and function in relation to cardiovascular risk factors
controls. The expression of CD11b and CD63 on granulocytes and monocytes were measured by flow cytometry. RV diastolic functions were assessed by tricuspid annular tissue Doppler recordings. Backscatter cyclic variation (CV) and maximal intensity (IB) were measured in RV lateral wall from parasternal long-axis view. Results: The mean fluorescence intensity (MFI) of CD11b (45.3±17.8 vs 20.2±5.6, p<0.001) on granulocytes and CD63 (2.3±0.69 vs 1.5±0.28, p<0.001) on monocytes in BD patients were higher than those of controls. Patients with BD had smaller tricuspid annular early diastolic wave velocities (8.6±1.6 cm/s vs 10.0±1.7 cm/s, p=0.01) and early/late diastolic wave velocity ratios (E/A) (1.2±0.03 vs 1.3±0.03, p<0.001) than controls. MFI of CD11b and CD63 were inversely correlated with tricuspid annular E/A in BD patients. CV of RV was found to be diminished in BD patients than controls (8.5±1.25 dB vs 11.5±1.45 dB, p<0.001). MFI of CD11b and CD63 showed an inverse relation with CV of RV in BD patients. Conclusion: There is an association between the levels of cellular adhesion molecules, deterioration of RV diastolic function and altered myocardial ultrasonic reflectivity in BD. PO14-377
ENDOTHELIN-1 IS ASSOCIATED WITH CALCIUM SCORE IN ASYMPTOMATIC SUBJECTS, AS DIAGNOSED BY 64-ROW MULTIDETECTOR COMPUTED TOMOGRAPHY
Y. Ben Moshe 1,2 , A.B.R. Zeina 1,2 , G.H. Zaid 1,2 , E. Rivlin 1,2 , I. Maor 1,2 , E. Bar- Meir 1,2 , U. Rosenschein 1,2 , E. Goldhammer 1,2 . 1 Departments of Cardiology, Cardiac Rehabilitation & Biochemistry, Bnai-Zion Medical Center, Haifa, Israel; 2 Faculty of Medicine, Technion Background: endothelin-1 has been implicated in the inflammatory process taking place in active atheromatous coronary lesions associated with acute coronary syndromes. Aim: to investigate the association between plasma endothelin-1 and the extent, type of lesions, and severity of coronary artery disease in asymptomatic subjects, as assessed by a multidetector computed tomography (MDCT). Methods: 1020 coronary segments of 68 subjcets, age = 59.2±11.23 years, 51m, and 17f, were reviewed. Coronary computed tomography angiography (CCTA) was performed using 64- row MDCT scanner (VCT, GE Medical, Milwaukee, USA). Image reconstruction was done using the retrospective ECG gating method, and coronary arteries were reviewed using the AHA 15-segment model. Plaques were categorized as calcified, soft, or soft and calcified, and lesions as 0-25%, 25-50%, 50-75%, and 75-100% stenosis. Endothelin assessment was done using plasma EDTA human endothelin-1 immunoassay (R&D systems, Minneapolis, USA). Results: 76 lesions were soft, 11 calcified, and 251 soft and calcified. Endothelin correlated with Ca score, r = 0.39, p<0.001, similar correlation was found in patients with high Ca score and hypercholesterolemia, r = 0.44, p = 0.008. Significant correlation was found between Ca score, number of plaques and age, r = 0.52, p<0.001, and r= 0.6, p<0.0001. No correlation was found with sex, hypertension, smoking, previous infarction, percutaneous intervention, bypass surgery, number of plaques or percentage of stenosis. Conclusions: In non acute settings, endothelin-1 correlates with the presence of high calcium score as assessed by MDCT. It does not reflect probably the extent and severity of the disease. PO14-378
PREVALENCE OF STIFFNESS OF LARGE AND SMALL ARTERIES IN PATIENTS WITH ELEVATED CARDIOVASCULAR RISK
O. Sanchez, R. Fabregate, M. Fabregate, D. Coca-Robinot, A. Amador, J. Saban-Ruiz. Endothelial Pathology Unit, Internal Medicine Service, Ramon y Cajal Hospital, Madrid, Spain Introduction: The HDI/PulseWave™ CR-2000 Research Cardiovascular Profiling System obtains upper-arm blood pressure values and waveform data by non-invasive methods. Aims: 1-To determine the prevalence of abnormal large and/or small artery elasticity index. 2.-To determine its correlation with constitutional and biochemical factors, and hemodynamic characteristics. Methods: N=107, 27-81yrs (59,05±1,34), 54,5% M, 72 hypertensive,BMI (kg/m2 ) and waist (cm). Gly, creat, HDL, LDL, chol, TG, by Hitachi. Systolic BP (SBP), diastolic BP (DBP), mean BP (MBP), pulse pressure (PP); large (C1) and small (C2) artery elasticity index, systemic
vascular resistance (SVR), total vascular impedance (TVI), Cardiac output (CO), Stroke volume (SV) by HDI/PulseWave CR-2000. Statistical analysis: t-Student, Levene’s and Mann-Whitney Utest. Results: 1. The prevalence of abnormal large artery elasticity (C1) was 38,3%. These patients were older (p=0,001) and presented higher values in SBP (p<0,001), DBP (p=0,004), MBP (p<0,001) and PP (p<0,001). 2. In relation to C1 we also found significant differences in SVR (p<0,001), TVI (p<0,001), CO (p<0,001), and SV (p<0,001). 3. The prevalence of abnormal small artery elasticity (C2) was 43,9%. These patients were older (p<0,001) and had higher SBP (p=0,02), DBP (p=0,014), MBP (p<0,001), PP (p=0,010). 4. Related to C2 we found significant differences in SVR (p<0,001), TVI (p=0,007), CO (p=0,001), SV (p=0,004). 5. There is a relation between small artery elasticity and triglycerides levels, with higher values in abnormal C2 group (p=0,007). Conclusions: 1. The prevalence of stiffness of large and small arteries was of 38,3% and 43,9% respectively. 2. Both phenomenon go with changes in other cardiovascular parameters when they are evaluated by a novel research cardiovascular profiling system. 3. The stiffness of small arteries is related to levels of TG. This could suggest a new possible pathophysiological relation between vascular damage and hypertriglyceridemia. PO14-379
RELATION BETWEEN THE STIFFNESS OF LARGE/SMALL ARTERIES AND ENDOTHELIUM-DEPENDENT AND NON-ENDOTHELIUM DEPENDENT VASODILATION
J. Saban-Ruiz, R. Fabregate, M. Fabregate, O. Sanchez, S. Redondo, N. De La Torre, J. Campoy. Endothelial Pathology Unit, Internal Medicine Service, Ramon y Cajal Hospital, Madrid, Spain Introduction: The elasticity of blood vessels may be obtained by a novel non-invasive method using an HDI/PulseWave CR-2000 Research Cardiovascular Profiling System. Aims: 1. To determine the prevalence of abnormal large and/or small artery elasticity index in a high cardiovascular risk population. 2. To determine its correlation with macrovascular and/or microvascular endothelial dysfunction. Methods: N=94 patients, 27-81 yrs (58,83±1,44 SEM), 52,8% M, 61 hypertensives. Large (C1) and small (C2) artery elasticity index: HDI/PulseWave CR-2000. Macrovascular study: Endothelium (ED) and non-endothelium (NED) dependent vasodilation. Celermajer method (Flow Mediated dilation – FMD – and hyperemia). Microvascular study: DRT4, MOOR Instruments: Speed Relative Variation respect to Baseline (SRVB) (%). Peak-Flow Relative Variation respect to Isquemia-flow (PFRVI). Statistical analysis: t-student, chi-square and Mann-Whitney U test. Results: 1-The prevalence of abnormal large artery elasticity was 37,2%. 2-The prevalence of abnormal small artery elasticity was 43,9%. 3-We found that patients with this abnormal large or small artery elasticity were older that those with a normal result (p <0,001). 4-When assessed with the Celermajer method these patients had a smaller post-ischaemic hyperemia response (p=0,036) as well as a smaller vasodilating response to nitroglycerin (p=0,033). 5-In the group of patients with an abnormal C2 result, we found a smaller PFRVI (p=0,008). Conclusions: 1-The prevalence of stiffness in large and small arteries was of 37,2% y 43,9%, respectively. 2-We found a relation between the stiffness of large arteries and ED and NED vasodilation. 3-The stiffness of small arteries is related to a smaller ED vasodilation. PO14-380
CAROTID DILATATION AND CHOLESTEROLEMIA AT HYPERTENSIVE PATIENTS AS PREDICTOR FOR STROKE
B. Pojskic 1 , A. Macic-Dzankovic 2 . 1 Cardiology Department,Cantonal Hospital Zenica, Zenica, Bosnia and Herzegovina; 2 Department of Internal Diseases,General Hospital Sarajevo, Sarajevo, Bosnia and Herzegovina Backgrounds: Hypertension(HTA)and increased cholesterol are important risk factors in the development of atherosclerosis. However,it is not clear which degree of other structural changes in CCA and level of cholesterol in HTA are most likely to be predictor for CVD future events. Aim: Estimate cholesterol serum level and diametar (D) CCA as predictor for CVD future events in HTA group Methods: Noninvasive ultrasonography measurements of DCCA were made in two group of pts with HTA, GroupI with hystory of previous
76th Congress of the European Atherosclerosis Society, June 10–13, 2007, Helsinki, Finland
Poster Sessions PO14 Arterial structure and function in relation to cardiovascular risk factors
controls. The expression of CD11b and CD63 on granulocytes and monocytes were measured by flow cytometry. RV diastolic functions were assessed by tricuspid annular tissue Doppler recordings. Backscatter cyclic variation (CV) and maximal intensity (IB) were measured in RV lateral wall from parasternal long-axis view. Results: The mean fluorescence intensity (MFI) of CD11b (45.3±17.8 vs 20.2±5.6, p<0.001) on granulocytes and CD63 (2.3±0.69 vs 1.5±0.28, p<0.001) on monocytes in BD patients were higher than those of controls. Patients with BD had smaller tricuspid annular early diastolic wave velocities (8.6±1.6 cm/s vs 10.0±1.7 cm/s, p=0.01) and early/late diastolic wave velocity ratios (E/A) (1.2±0.03 vs 1.3±0.03, p<0.001) than controls. MFI of CD11b and CD63 were inversely correlated with tricuspid annular E/A in BD patients. CV of RV was found to be diminished in BD patients than controls (8.5±1.25 dB vs 11.5±1.45 dB, p<0.001). MFI of CD11b and CD63 showed an inverse relation with CV of RV in BD patients. Conclusion: There is an association between the levels of cellular adhesion molecules, deterioration of RV diastolic function and altered myocardial ultrasonic reflectivity in BD. PO14-377
ENDOTHELIN-1 IS ASSOCIATED WITH CALCIUM SCORE IN ASYMPTOMATIC SUBJECTS, AS DIAGNOSED BY 64-ROW MULTIDETECTOR COMPUTED TOMOGRAPHY
Y. Ben Moshe 1,2 , A.B.R. Zeina 1,2 , G.H. Zaid 1,2 , E. Rivlin 1,2 , I. Maor 1,2 , E. Bar- Meir 1,2 , U. Rosenschein 1,2 , E. Goldhammer 1,2 . 1 Departments of Cardiology, Cardiac Rehabilitation & Biochemistry, Bnai-Zion Medical Center, Haifa, Israel; 2 Faculty of Medicine, Technion Background: endothelin-1 has been implicated in the inflammatory process taking place in active atheromatous coronary lesions associated with acute coronary syndromes. Aim: to investigate the association between plasma endothelin-1 and the extent, type of lesions, and severity of coronary artery disease in asymptomatic subjects, as assessed by a multidetector computed tomography (MDCT). Methods: 1020 coronary segments of 68 subjcets, age = 59.2±11.23 years, 51m, and 17f, were reviewed. Coronary computed tomography angiography (CCTA) was performed using 64- row MDCT scanner (VCT, GE Medical, Milwaukee, USA). Image reconstruction was done using the retrospective ECG gating method, and coronary arteries were reviewed using the AHA 15-segment model. Plaques were categorized as calcified, soft, or soft and calcified, and lesions as 0-25%, 25-50%, 50-75%, and 75-100% stenosis. Endothelin assessment was done using plasma EDTA human endothelin-1 immunoassay (R&D systems, Minneapolis, USA). Results: 76 lesions were soft, 11 calcified, and 251 soft and calcified. Endothelin correlated with Ca score, r = 0.39, p<0.001, similar correlation was found in patients with high Ca score and hypercholesterolemia, r = 0.44, p = 0.008. Significant correlation was found between Ca score, number of plaques and age, r = 0.52, p<0.001, and r= 0.6, p<0.0001. No correlation was found with sex, hypertension, smoking, previous infarction, percutaneous intervention, bypass surgery, number of plaques or percentage of stenosis. Conclusions: In non acute settings, endothelin-1 correlates with the presence of high calcium score as assessed by MDCT. It does not reflect probably the extent and severity of the disease. PO14-378
PREVALENCE OF STIFFNESS OF LARGE AND SMALL ARTERIES IN PATIENTS WITH ELEVATED CARDIOVASCULAR RISK
O. Sanchez, R. Fabregate, M. Fabregate, D. Coca-Robinot, A. Amador, J. Saban-Ruiz. Endothelial Pathology Unit, Internal Medicine Service, Ramon y Cajal Hospital, Madrid, Spain Introduction: The HDI/PulseWave™ CR-2000 Research Cardiovascular Profiling System obtains upper-arm blood pressure values and waveform data by non-invasive methods. Aims: 1-To determine the prevalence of abnormal large and/or small artery elasticity index. 2.-To determine its correlation with constitutional and biochemical factors, and hemodynamic characteristics. Methods: N=107, 27-81yrs (59,05±1,34), 54,5% M, 72 hypertensive,BMI (kg/m2 ) and waist (cm). Gly, creat, HDL, LDL, chol, TG, by Hitachi. Systolic BP (SBP), diastolic BP (DBP), mean BP (MBP), pulse pressure (PP); large (C1) and small (C2) artery elasticity index, systemic
vascular resistance (SVR), total vascular impedance (TVI), Cardiac output (CO), Stroke volume (SV) by HDI/PulseWave CR-2000. Statistical analysis: t-Student, Levene’s and Mann-Whitney Utest. Results: 1. The prevalence of abnormal large artery elasticity (C1) was 38,3%. These patients were older (p=0,001) and presented higher values in SBP (p<0,001), DBP (p=0,004), MBP (p<0,001) and PP (p<0,001). 2. In relation to C1 we also found significant differences in SVR (p<0,001), TVI (p<0,001), CO (p<0,001), and SV (p<0,001). 3. The prevalence of abnormal small artery elasticity (C2) was 43,9%. These patients were older (p<0,001) and had higher SBP (p=0,02), DBP (p=0,014), MBP (p<0,001), PP (p=0,010). 4. Related to C2 we found significant differences in SVR (p<0,001), TVI (p=0,007), CO (p=0,001), SV (p=0,004). 5. There is a relation between small artery elasticity and triglycerides levels, with higher values in abnormal C2 group (p=0,007). Conclusions: 1. The prevalence of stiffness of large and small arteries was of 38,3% and 43,9% respectively. 2. Both phenomenon go with changes in other cardiovascular parameters when they are evaluated by a novel research cardiovascular profiling system. 3. The stiffness of small arteries is related to levels of TG. This could suggest a new possible pathophysiological relation between vascular damage and hypertriglyceridemia. PO14-379
RELATION BETWEEN THE STIFFNESS OF LARGE/SMALL ARTERIES AND ENDOTHELIUM-DEPENDENT AND NON-ENDOTHELIUM DEPENDENT VASODILATION
J. Saban-Ruiz, R. Fabregate, M. Fabregate, O. Sanchez, S. Redondo, N. De La Torre, J. Campoy. Endothelial Pathology Unit, Internal Medicine Service, Ramon y Cajal Hospital, Madrid, Spain Introduction: The elasticity of blood vessels may be obtained by a novel non-invasive method using an HDI/PulseWave CR-2000 Research Cardiovascular Profiling System. Aims: 1. To determine the prevalence of abnormal large and/or small artery elasticity index in a high cardiovascular risk population. 2. To determine its correlation with macrovascular and/or microvascular endothelial dysfunction. Methods: N=94 patients, 27-81 yrs (58,83±1,44 SEM), 52,8% M, 61 hypertensives. Large (C1) and small (C2) artery elasticity index: HDI/PulseWave CR-2000. Macrovascular study: Endothelium (ED) and non-endothelium (NED) dependent vasodilation. Celermajer method (Flow Mediated dilation – FMD – and hyperemia). Microvascular study: DRT4, MOOR Instruments: Speed Relative Variation respect to Baseline (SRVB) (%). Peak-Flow Relative Variation respect to Isquemia-flow (PFRVI). Statistical analysis: t-student, chi-square and Mann-Whitney U test. Results: 1-The prevalence of abnormal large artery elasticity was 37,2%. 2-The prevalence of abnormal small artery elasticity was 43,9%. 3-We found that patients with this abnormal large or small artery elasticity were older that those with a normal result (p <0,001). 4-When assessed with the Celermajer method these patients had a smaller post-ischaemic hyperemia response (p=0,036) as well as a smaller vasodilating response to nitroglycerin (p=0,033). 5-In the group of patients with an abnormal C2 result, we found a smaller PFRVI (p=0,008). Conclusions: 1-The prevalence of stiffness in large and small arteries was of 37,2% y 43,9%, respectively. 2-We found a relation between the stiffness of large arteries and ED and NED vasodilation. 3-The stiffness of small arteries is related to a smaller ED vasodilation. PO14-380
CAROTID DILATATION AND CHOLESTEROLEMIA AT HYPERTENSIVE PATIENTS AS PREDICTOR FOR STROKE
B. Pojskic 1 , A. Macic-Dzankovic 2 . 1 Cardiology Department,Cantonal Hospital Zenica, Zenica, Bosnia and Herzegovina; 2 Department of Internal Diseases,General Hospital Sarajevo, Sarajevo, Bosnia and Herzegovina Backgrounds: Hypertension(HTA)and increased cholesterol are important risk factors in the development of atherosclerosis. However,it is not clear which degree of other structural changes in CCA and level of cholesterol in HTA are most likely to be predictor for CVD future events. Aim: Estimate cholesterol serum level and diametar (D) CCA as predictor for CVD future events in HTA group Methods: Noninvasive ultrasonography measurements of DCCA were made in two group of pts with HTA, GroupI with hystory of previous
76th Congress of the European Atherosclerosis Society, June 10–13, 2007, Helsinki, Finland