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Polymorphonuclear leukocyte responses in adjuvant arthritis rats.
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References Silva, P.M.R. et al., 1989, Brazilian J. Med. Biol. Res. 22, 1281. Martins, M.A. et al., 1989, Br. J. Pharmacol., 96, 363. Leprovost, C. et al., 1988, Int. Arch. Allergy Appl. Immunol., 87, 9.
P.fr.149] Polymorphonudear leukocyte responses in adjuvant arthritis rats. Nose, T., Segawa, Y., Nozaki, M., Tsurumi, K. a n d Fujimura *, H. Department of Pharmacology, Gifu UniversitySchool of Medicine, Gifu 500 and * Research Institute for Production Development. Kyoto 606, Japan
In acute inflammation, peripheral blood polymorphonuclear leukocytes (PMNs) play important roles; migrating to extravascular tissue and so on. In this paper, the functional and morphological changes of PMNs in blood were evaluated in adjuvant-induced arthritis rats which were performed by injection of a suspension consisting of liquid paraffin and mycobacterium (0.5rag) into the plantar region of the hind-paw. A primary swelling of the injected foot reached a maximum 3 days after the injection (day 3), followed by a more pronounced chronic swelling (secondary inflammation) which started from day 10. The peripheral PMNs gradually increased with development of the arthritis; 4.6 times at day 10, compared to normal animals and the increments were maintained up to day 21. 1. Adhesion of PMNs to the surface of slide glass: Plasma and buffy coat fraction were individually removed from whole blood. The plasma was placed on a well of a tissue culture chamber slide and then the buffy coat was overlaid. After the incubation for 60 mitt, the slide was washed with Eagle's ME-mediun'l. Adhesive PMNs to the slide were counted. The PMN-adhesion was enhanced depending on the development of the arthritis. The maximal response was observed at day 10 when swelling in the non-injected hind-paw began. 2. NBT dye reducing capa~ty: The slide with adhesive FMNs was incubated with NBT (nitroblue tetrazc,lium) solution for 20 min. The reducing capacity increased with a peak at day 14. 3. Luminol-dependent chemiluminescence (CL): The enhancement of CL induced by zymosan m the ~uffy coat preparation was detected through 3 days after the adjuvant injection. However, the CL-development from day 7 was lower than the normal level. 4. Scanning electron microscopic study of PMNs: The PMNs from normal animals were almost spherical. At day 7, the PMNs from the arthritis rats were spread out with pseudopodia; migration. A multiple adhesion to PMN was observed from 10 to 21 days depending on the development of the arthritis. P.fr.150 ]
Participation of myeioperoxidase in phagocytosis of rat neutrophi!s Lee, E., Fujita, M. and Kariya, K. Department of Pharmacology, Faculty of PharmaceuticalSciences, Kobe-Gakuin University, lkawadani-cho, Nishi-ku, Kobe 673, Japan
Myeloperoxidase is present in bone marrow cells and is a marker in the neutrophilic granulocytes. We previously reported the purification and characterization of myeloperoxidase and eosinophil peroxidase from rat bone marrow (Kariya et al., 1987), which were participated in oxidative metabolism of the drugs (Lee et al., 1988). Recently we determined the level of myeloperoxidase of bone marrow in various strains of mice and showed the relationship between the level ~md the catalytic activity of the peroxidase. Myeloperoxidase is also observed in mammalian neutrophils and is thought to be involved in the bacteriocidal function. However, little is known about a role of myeloperoxidase in phagocytosis of neutrophils. Here, we examined the phagocytosis by flow cytometric analysis and discussed a participation of myeloperoxidase in the neutrophilic phagocytosis.
References Silva, P.M.R. et al., 1989, Brazilian J. Med. Biol. Res. 22, 1281. Martins, M.A. et al., 1989, Br. J. Pharmacol., 96, 363. Leprovost, C. et al., 1988, Int. Arch. Allergy Appl. Immunol., 87, 9.
P.fr.149] Polymorphonudear leukocyte responses in adjuvant arthritis rats. Nose, T., Segawa, Y., Nozaki, M., Tsurumi, K. a n d Fujimura *, H. Department of Pharmacology, Gifu UniversitySchool of Medicine, Gifu 500 and * Research Institute for Production Development. Kyoto 606, Japan
In acute inflammation, peripheral blood polymorphonuclear leukocytes (PMNs) play important roles; migrating to extravascular tissue and so on. In this paper, the functional and morphological changes of PMNs in blood were evaluated in adjuvant-induced arthritis rats which were performed by injection of a suspension consisting of liquid paraffin and mycobacterium (0.5rag) into the plantar region of the hind-paw. A primary swelling of the injected foot reached a maximum 3 days after the injection (day 3), followed by a more pronounced chronic swelling (secondary inflammation) which started from day 10. The peripheral PMNs gradually increased with development of the arthritis; 4.6 times at day 10, compared to normal animals and the increments were maintained up to day 21. 1. Adhesion of PMNs to the surface of slide glass: Plasma and buffy coat fraction were individually removed from whole blood. The plasma was placed on a well of a tissue culture chamber slide and then the buffy coat was overlaid. After the incubation for 60 mitt, the slide was washed with Eagle's ME-mediun'l. Adhesive PMNs to the slide were counted. The PMN-adhesion was enhanced depending on the development of the arthritis. The maximal response was observed at day 10 when swelling in the non-injected hind-paw began. 2. NBT dye reducing capa~ty: The slide with adhesive FMNs was incubated with NBT (nitroblue tetrazc,lium) solution for 20 min. The reducing capacity increased with a peak at day 14. 3. Luminol-dependent chemiluminescence (CL): The enhancement of CL induced by zymosan m the ~uffy coat preparation was detected through 3 days after the adjuvant injection. However, the CL-development from day 7 was lower than the normal level. 4. Scanning electron microscopic study of PMNs: The PMNs from normal animals were almost spherical. At day 7, the PMNs from the arthritis rats were spread out with pseudopodia; migration. A multiple adhesion to PMN was observed from 10 to 21 days depending on the development of the arthritis. P.fr.150 ]
Participation of myeioperoxidase in phagocytosis of rat neutrophi!s Lee, E., Fujita, M. and Kariya, K. Department of Pharmacology, Faculty of PharmaceuticalSciences, Kobe-Gakuin University, lkawadani-cho, Nishi-ku, Kobe 673, Japan
Myeloperoxidase is present in bone marrow cells and is a marker in the neutrophilic granulocytes. We previously reported the purification and characterization of myeloperoxidase and eosinophil peroxidase from rat bone marrow (Kariya et al., 1987), which were participated in oxidative metabolism of the drugs (Lee et al., 1988). Recently we determined the level of myeloperoxidase of bone marrow in various strains of mice and showed the relationship between the level ~md the catalytic activity of the peroxidase. Myeloperoxidase is also observed in mammalian neutrophils and is thought to be involved in the bacteriocidal function. However, little is known about a role of myeloperoxidase in phagocytosis of neutrophils. Here, we examined the phagocytosis by flow cytometric analysis and discussed a participation of myeloperoxidase in the neutrophilic phagocytosis.