- Email: [email protected]
Population Data
Forensic Science International 126 (2002) 171±172
Announcement of population data
Population data on the X chromosome short tandem repeat loci HPRTB and AR in Taiwan Meng-Yi Chen, Fang-Chin Wu, Lin-Ming Meng, Shiu-Ming Cheng, Chi-Hsiang Chao, Chang-En Pu* Scienti®c and Technical Research Center, Ministry Justice Investigation Bureau, 74 Chung-Hua Road, Hsin-Tien City, Taipei County, 231, Taiwan, ROC Received 14 December 2001; received in revised form 20 January 2002; accepted 13 February 2002
Abstract This report contains the results of population studies on the X chromosome STR HPRTB and AR carried out in Taiwan. The numbers of unrelated individuals were 428: female 143 and male 285 for HPRTB locus, and 416: female 142 and male 274 for AR locus. Keywords: Forensic science; Short tandem repeats; X chromosome; HPRTB; AR; Population data
Population: Unrelated volunteer donors living in Taiwan. Extraction: DNA was extracted by using BioGeneFastTM 30 min DNA extraction kit (Texas BioGene Inc., TA, USA) and quantitated by a Fluorometer (DyNA Quant 200, Hoefer Pharmacia Biotech, San Francisco, CA, USA). PCR: PCR ampli®cation was performed by using 50 ng genomic DNA in a 25 ml reaction volume comprising 2.5 ml 10x STR buffer (Gold STR, Promega, Madison, WI, USA), 2.5 U Tag Gold Polymerase (Applied Biosystems, Foster City, CA USA), 1 mM AR primer and 1 mM HPRTB primer. Forward primers were 50 labeled with the following dyes: FAM, HEX to make the diplex PCR. PCR cycling conditions are, 95 8CÐ11 min, 30 cycles of 94 8CÐ1 min, 58 8CÐ
1 min and 72 8CÐ1 min, 60 8CÐ45 min, 4 8CÐforever, in a GeneAmp PCR System 9600 (Applied Biosystems, USA) Thermal Cycler. Each of the most common allele of each locus was sequenced to con®rm the number of the repeat units. Typing: ABI 310 (Applied Biosystem) with sequenced ladders referred from [1±3] and made by authors. Results: See Table 1. Analysis of data: POPGENE [4] and PowerStat Excel Template [5]. Access to the data: Email to [email protected]. Other remarks: The authors wish to thank Mr. Wu, GuoChun for his kindly help in DNA extraction, quantitation and sequencer handling.
* Corresponding author. Tel.: 886-2-2911-2241x3741; fax: 886-2-2913-8599. E-mail address: [email protected] (C.-E. Pu).
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M.-Y. Chen et al. / Forensic Science International 126 (2002) 171±172
Table 1 The allele frequencies for STR HPRTB and AR in a chinese population in Taiwan Allele
11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 39 H PD CE p
AR
HPRTB
Male
Female
Total
Male
Female
Total
0.0036 ± 0.0073 ± 0.0255 0.0146 0.0219 0.0255 0.0438 0.0584 0.0803 0.1971 0.1058 0.1168 0.1131 0.0803 0.0620 0.0109 0.0073 0.0073 0.0036 0.0036 ± 0.0036 0.0073 ± ± ± ±
± 0.0035 ± ± 0.0284 0.0213 0.0142 0.0106 0.0532 0.0745 0.0993 0.1631 0.0745 0.1170 0.0887 0.0851 0.0993 0.0213 0.0213 0.0035 ± 0.0142 0.0035 0.0035 ± 0.919 0.976 0.835 0.9960
0.0018 0.0018 0.0036 ± 0.0270 0.0180 0.0180 0.0180 0.0486 0.0667 0.0901 0.1802 0.0901 0.1171 0.1009 0.0829 0.0793 0.0162 0.0144 0.0054 0.0018 0.0090 0.0018 0.0036 0.0036 ± ± ± ±
0.0491 0.2877 0.4140 0.2070 0.0421 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±
0.0979 0.3111 0.3846 0.1643 0.0350 0.0070 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.762 0.866 0.531 0.7759
0.0736 0.2995 0.3993 0.1856 0.0385 0.0035 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±
H: observed heterozigosity, PD: power of discrimination, CE: a priori chance of exclusion, p: likelihood ratio test for Hardy±Weinberg equilibrium.
References [1] F.X. Xiao, A. Gilissen, J.J. Cassiman, R. Decorte, Quadruplex ¯uorescent STR typing system (HUMVWA HUMTH01 D21S11 and HPRT) with sequence-de®ned allelic ladders, Identi®cation of a new allele at D21S11, Forensic Sci. Int. 94 (1998) 39±46. [2] R. Szibor, S. Lautsch, I. Plate, N. Beck, Population data on the X chromosome short tandem repeat locus HPRTB in two regions of Germany, J. Forensic Sci. 45 (1) (2000) 231±233.
[3] T. Kishida, Y. Tamaki, Japanese population data on Xchromosomal STR locus AR, Jpn. J. Legal Med. 51 (1997) 376±379. [4] POPGENE, software distributed on the Internet for Population Genetic Analysis, A Joint Project of Ag for Molecular Biology and Biotechnology Centre, Center for International Forestry Research, University of Alberta. [5] A. Tereba, Tools for analysis of population statistics, Pro®les in DNA 2 (1999) 14±16.
Announcement of population data
Population data on the X chromosome short tandem repeat loci HPRTB and AR in Taiwan Meng-Yi Chen, Fang-Chin Wu, Lin-Ming Meng, Shiu-Ming Cheng, Chi-Hsiang Chao, Chang-En Pu* Scienti®c and Technical Research Center, Ministry Justice Investigation Bureau, 74 Chung-Hua Road, Hsin-Tien City, Taipei County, 231, Taiwan, ROC Received 14 December 2001; received in revised form 20 January 2002; accepted 13 February 2002
Abstract This report contains the results of population studies on the X chromosome STR HPRTB and AR carried out in Taiwan. The numbers of unrelated individuals were 428: female 143 and male 285 for HPRTB locus, and 416: female 142 and male 274 for AR locus. Keywords: Forensic science; Short tandem repeats; X chromosome; HPRTB; AR; Population data
Population: Unrelated volunteer donors living in Taiwan. Extraction: DNA was extracted by using BioGeneFastTM 30 min DNA extraction kit (Texas BioGene Inc., TA, USA) and quantitated by a Fluorometer (DyNA Quant 200, Hoefer Pharmacia Biotech, San Francisco, CA, USA). PCR: PCR ampli®cation was performed by using 50 ng genomic DNA in a 25 ml reaction volume comprising 2.5 ml 10x STR buffer (Gold STR, Promega, Madison, WI, USA), 2.5 U Tag Gold Polymerase (Applied Biosystems, Foster City, CA USA), 1 mM AR primer and 1 mM HPRTB primer. Forward primers were 50 labeled with the following dyes: FAM, HEX to make the diplex PCR. PCR cycling conditions are, 95 8CÐ11 min, 30 cycles of 94 8CÐ1 min, 58 8CÐ
1 min and 72 8CÐ1 min, 60 8CÐ45 min, 4 8CÐforever, in a GeneAmp PCR System 9600 (Applied Biosystems, USA) Thermal Cycler. Each of the most common allele of each locus was sequenced to con®rm the number of the repeat units. Typing: ABI 310 (Applied Biosystem) with sequenced ladders referred from [1±3] and made by authors. Results: See Table 1. Analysis of data: POPGENE [4] and PowerStat Excel Template [5]. Access to the data: Email to [email protected]. Other remarks: The authors wish to thank Mr. Wu, GuoChun for his kindly help in DNA extraction, quantitation and sequencer handling.
* Corresponding author. Tel.: 886-2-2911-2241x3741; fax: 886-2-2913-8599. E-mail address: [email protected] (C.-E. Pu).
0379-0738/02/$ ± see front matter PII: S 0 3 7 9 - 0 7 3 8 ( 0 2 ) 0 0 0 4 9 - X
172
M.-Y. Chen et al. / Forensic Science International 126 (2002) 171±172
Table 1 The allele frequencies for STR HPRTB and AR in a chinese population in Taiwan Allele
11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 39 H PD CE p
AR
HPRTB
Male
Female
Total
Male
Female
Total
0.0036 ± 0.0073 ± 0.0255 0.0146 0.0219 0.0255 0.0438 0.0584 0.0803 0.1971 0.1058 0.1168 0.1131 0.0803 0.0620 0.0109 0.0073 0.0073 0.0036 0.0036 ± 0.0036 0.0073 ± ± ± ±
± 0.0035 ± ± 0.0284 0.0213 0.0142 0.0106 0.0532 0.0745 0.0993 0.1631 0.0745 0.1170 0.0887 0.0851 0.0993 0.0213 0.0213 0.0035 ± 0.0142 0.0035 0.0035 ± 0.919 0.976 0.835 0.9960
0.0018 0.0018 0.0036 ± 0.0270 0.0180 0.0180 0.0180 0.0486 0.0667 0.0901 0.1802 0.0901 0.1171 0.1009 0.0829 0.0793 0.0162 0.0144 0.0054 0.0018 0.0090 0.0018 0.0036 0.0036 ± ± ± ±
0.0491 0.2877 0.4140 0.2070 0.0421 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±
0.0979 0.3111 0.3846 0.1643 0.0350 0.0070 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.762 0.866 0.531 0.7759
0.0736 0.2995 0.3993 0.1856 0.0385 0.0035 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±
H: observed heterozigosity, PD: power of discrimination, CE: a priori chance of exclusion, p: likelihood ratio test for Hardy±Weinberg equilibrium.
References [1] F.X. Xiao, A. Gilissen, J.J. Cassiman, R. Decorte, Quadruplex ¯uorescent STR typing system (HUMVWA HUMTH01 D21S11 and HPRT) with sequence-de®ned allelic ladders, Identi®cation of a new allele at D21S11, Forensic Sci. Int. 94 (1998) 39±46. [2] R. Szibor, S. Lautsch, I. Plate, N. Beck, Population data on the X chromosome short tandem repeat locus HPRTB in two regions of Germany, J. Forensic Sci. 45 (1) (2000) 231±233.
[3] T. Kishida, Y. Tamaki, Japanese population data on Xchromosomal STR locus AR, Jpn. J. Legal Med. 51 (1997) 376±379. [4] POPGENE, software distributed on the Internet for Population Genetic Analysis, A Joint Project of Ag for Molecular Biology and Biotechnology Centre, Center for International Forestry Research, University of Alberta. [5] A. Tereba, Tools for analysis of population statistics, Pro®les in DNA 2 (1999) 14±16.