Population data of Y-chromosomal STRs in Russian males of the Primorye region population

Forensic Science International 159 (2006) 71–76 www.elsevier.com/locate/forsciint

Announcement of population data

Population data of Y-chromosomal STRs in Russian males of the Primorye region population Ru¨diger Lessig a,*, Jeanett Edelmann a, Werner J. Kleemann a, Valeri Kozhemyako b b

a Institute of Legal Medicine, University of Leipzig, Johannisallee 28, 04103 Leipzig, Germany Institute of Bioorganic Chemistry Far East Branch of Russian Academy of Sciences, 159 Stoletya St, Vladivostok, Russia

Received 31 January 2005; received in revised form 10 May 2005; accepted 10 May 2005 Available online 17 June 2005

Abstract Data of eight Y-chromosomal STRs, the so called ‘‘minimal core set’’, were obtained from 152 unrelated males of the Primorye region of Russia. The allelic frequencies correspond to other European populations. The background is a settlement of males from the European part of Russia, Ukraine and other states which were included in the former western part of the Soviet Union. On the other hand the distribution of the most frequent haplotypes differs to the Ukraine and Russian population. The most frequent haplotype was obtained five times in the population corresponding to 3.3%. The haplotype data reported here have been included into the Y-STR database maintained at the Institute of Legal Medicine, Humboldt-University, Berlin. # 2005 Elsevier Ireland Ltd. All rights reserved. Keywords: Population data; Short tandem repeat; Y-chromosome; Primorye region

1. Population

3. Primer and PCR

One hundred and fifty two unrelated males from the Primorye region of Russia (Fig. 1).

Following the manufacturer’s instructions for the Mentype1 Argus Y-MH kit (Biotype AG Dresden/Germany) in one multiplex reaction containing the eight Y-STRs of the ‘‘minimal core set’’ [4].

2. Extraction 4. Typing Qiagen extraction using the QIAamp DNA Blood Kit (Qiagen, Hilden/Germany).

* Corresponding author. Tel.: +49 341 9715118; fax: +49 341 9715109. E-mail address: [email protected] (R. Lessig).

The products were analysed using capillary electrophoresis (ABI Prism 3100 Avant DNA Sequencer, Applied Biosystems) in denaturing polymer (POP4). Allele assignment was performed by comparison to an allelic ladder (Biotype Dresden/Germany). The nomenclature for all loci follows the recommendations of the Y-STR Haplotype Reference Database [5].

0379-0738/$ – see front matter # 2005 Elsevier Ireland Ltd. All rights reserved. doi:10.1016/j.forsciint.2005.05.018

72

R. Lessig et al. / Forensic Science International 159 (2006) 71–76

Fig. 1. Map of the Primorye Region.

5. Data analysis

9. Other remarks

Allele frequencies were estimated by gene counting. The exclusion P power was calculated according to Nei [3] P(Y) P = (Pi)2, the haplotype diversity index h j ¼ ð1
fi2j Þ  N j =ðN j
1Þ to Takayama [6].

The population shows a distribution with low frequencies for most of the common minimal haplotpyes. The total haplotype diversity was estimated with 0.997 and the power of discrimination with 0.990. The power of discrimination of the analysed markers varies between 0.357 (DYS393) and 0.866 (DYS385). This is similar to other studies of populations from Russia and Ukraine [1]. Comparing the distribution of the most represented haplotpyes it differs in a small range to the populations of Ukraine (Kiev) and Russia (Moscow). The most common haplotype in the Primorye population is 15-13-30-25-11-11-13-11-14, which is not frequent in the Ukraine and Russian population. In the Ukraine population the most frequent haplotpye is 17-1330-25-11-11-13-10-14 and in the Russian population 16-1330-25-11-11-13-11-14. One duplication was found in DYS385 resulting in the genotype 10-11-14. The paper follows the guidelines for publication of population data requested by Forensic Science International [2].

6. Results See Tables 1 and 2. 7. Access to the data (haplotypes) http://www.yhrd.org.

8. Quality control Quality control exercise for Y-STRs organized by Dr. Lutz Roewer, Institute of Legal Medicine, Berlin.

R. Lessig et al. / Forensic Science International 159 (2006) 71–76

73

Table 1 Allele-/genotype frequencies of the Y-STRs in the Russian population Allele 9 10 11 12 13 14 15 16 17 19 22 23 24 25 26 27 28 29 30 31 32 33 Total PD

DYS19

0.079 0.263 0.224 0.322 0.112

DYS389 I

DYS389 II

DYS390

DYS391 0.007 0.493 0.480 0.020

0.013 0.105 0.697 0.184

DYS392

DYS393

0.770 0.039 0.079 0.099 0.013

0.007 0.066 0.789 0.125 0.013

0.007 0.066 0.250 0.257 0.375 0.046 0.020 0.086 0.199 0.483 0.146 0.060 0.007 1.000 0.758

1.000 0.468

1.000 0.694

1.000 0.725

1.000 0.525

1.000 0.390

Genotype

DYS385

Genotype

DYS385

10-11-14 10-14 10-15 10-18 11-11 11-13 11-14 11-15 11-16 12-12 12-13 12-14 12-15 12-16 12-17 13-13 13-14 13-15 13-16 13-16.3 13-17 14-14

0.007 0.013 0.007 0.007 0.013 0.119 0.291 0.146 0.013 0.013 0.020 0.020 0.007 0.007 0.007 0.013 0.040 0.020 0.013 0.013 0.020 0.033

14-15 14-16 15-16 16-17 16-18 16-20 17-19 19-19

0.079 0.007 0.020 0.026 0.007 0.007 0.007 0.013

1.000 0.357

1.000 0.868

Table 2 Haplotype frequencies of the Y-STRs in the Russian population DYS19

DYS389 I

DYS389 II

DYS390

DYS391

DYS392

DYS393

DYS385

Abs.

%

13 13 13 13 13 13 13 13 13 13 13 13 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14

13 13 13 14 13 13 13 14 13 14 13 13 13 13 13 13 12 12 12 12 12 13 13 12 12 12 13

30 30 30 32 30 30 31 31 31 29 29 30 30 28 29 29 27 28 28 28 30 29 30 28 28 29 29

24 24 24 24 25 25 25 25 23 23 23 23 22 23 23 24 22 22 22 22 22 22 22 23 23 23 23

10 10 10 10 10 10 10 10 10 11 10 10 10 10 11 11 10 10 10 10 10 10 10 10 10 10 10

11 11 11 11 11 11 11 11 12 12 13 12 11 11 13 13 11 11 11 11 11 11 11 11 11 11 11

13 13 13 13 13 13 13 13 13 13 13 14 12 12 12 12 13 13 13 13 13 13 13 13 13 13 13

16-17 16-17 16-18 16-20 19-19 19-19 16-17 17-19 12-12 13-17 13-16 12-12 13-15 13-17 11-14 11-15 12-14 12-13 13-14 13-15 14-14 13-15 13-14 14-14 14-15 13-14 14-14

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1

0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 1.3 0.7 0.7 0.7

74

R. Lessig et al. / Forensic Science International 159 (2006) 71–76

Table 2 (Continued ) DYS19

DYS389 I

DYS389 II

DYS390

DYS391

DYS392

DYS393

DYS385

Abs.

%

14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 16 16 16 16 16 16 16

13 12 12 13 14 13 14 13 13 13 14 14 14 14 14 13 14 14 14 14 14 14 13 14 14 13 13 13 12 12 12 13 13 13 13 13 11 13 13 13 13 13 13 13 13 13 14 13 14 14 13 13 11 13 13 14 13 13 13

30 29 28 30 31 29 30 29 29 30 30 31 30 30 30 28 30 31 30 30 29 30 30 31 31 29 30 29 28 28 28 29 30 29 30 32 27 30 31 32 30 30 30 30 30 30 31 29 32 30 29 29 27 30 31 30 29 29 29

24 22 23 24 23 24 19 23 24 24 23 23 24 23 23 23 23 23 24 22 23 23 24 23 23 23 23 25 24 25 23 24 24 25 25 25 26 24 24 24 25 25 25 25 26 25 24 24 23 23 23 23 24 24 24 24 25 25 25

10 11 11 11 10 10 11 11 11 11 10 10 10 11 12 10 10 10 10 11 11 11 11 10 10 9 10 10 11 11 10 10 10 10 10 10 10 11 11 11 11 11 11 11 11 10 10 11 10 10 11 10 10 10 10 10 10 10 10

11 11 11 11 13 13 13 13 13 13 14 14 14 14 11 14 14 14 14 14 14 14 14 15 14 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 12 13 13 12 14 15 14 11 11 11 11 11 11 11

13 13 13 13 13 13 13 13 13 13 13 13 13 13 14 14 14 14 14 14 14 14 14 14 11 12 12 12 12 12 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 14 14 14 15 13 13 13 13 13 13 13

16-17 13-13 14-14 11-15 14-16 11-14 13-13 12-14 11-11 11-14 11-13 11-14 11-15 12-13 11-13 12-13 11-13 11-13 11-13 11-14 11-13 11-13 11-13 11-13 12-16 13-16 12-17 11-14 13-16.3 13-17 13-14 11-14 13-14 11-14 11-14 12-15 11-15 11-15 11-14 14-15 10-11-14 11-14 12-14 11-15 11-14 11-14 11-14 11-15 14-15 11-13 11-11 11-13 11-14 11-14 14-15 11-14 11-13 11-14 11-15

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 1.3 0.7 0.7 0.7 0.7 0.7 1.3 0.7 3.3 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 2 1 5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

R. Lessig et al. / Forensic Science International 159 (2006) 71–76

75

Table 2 (Continued ) DYS19

DYS389 I

DYS389 II

DYS390

DYS391

DYS392

DYS393

DYS385

Abs.

%

16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 17 17 17 17 17 17 17 17 17 17 17 17 17 17

13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 14 12 14 14 13 14 13 13 13 14 13 12 13 13 13 13 13 13 14 13 13 13 13 13 13

30 30 30 31 30 30 31 30 30 30 30 31 32 29 29 30 30 31 30 31 31 29 31 32 30 29 30 30 31 32 30 28 30 30 30 30 32 31 33 29 30 30 30 30 30

25 25 25 25 26 23 23 24 24 24 24 24 24 25 25 25 25 25 25 25 25 26 26 25 26 23 23 23 25 25 25 24 24 25 25 25 23 24 24 25 25 25 25 26 24

10 10 10 10 10 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 12 12 10 11 11 11 11 11 10 10 10 10 10 11 11 11 11 11 11 11 11 11

11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 13 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 12

13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 14 14 14 14 15 12 13 13 13 13 13 13 13 13 13 13 13 13 13

11-13 11-14 11-15 14-15 11-14 11-14 15-15 11-13 11-14 11-15 14-15 14-15 15-15 11-14 11-15 11-14 11-15 11-14 11-16 14-15 11-14 11-14 11-14 11-15 11-16 10-18 14-15 15-16 11-15 11-15 11-15 13-16.3 10-14 10-15 11-13 11-14 14-15 11-13 13-14 11-13 11-13 11-14 11-15 11-14 11-15

1 3 1 1 1 1 1 1 1 1 1 2 1 3 1 3 4 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 2 1 1 3 1 1 1 1 1 1 1 1 1

0.7 2.0 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 1.3 0.7 2.0 0.7 2.0 2.6 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 1.3 0.7 0.7 0.7 1.3 0.7 0.7 2.0 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7

References [1] S.A. Kravchenko, P.A. Slominskii, L.A. Bets, A.V. Stepanova, A. Mikulich, S.A. Limborskaia, L.A. Livshits, Polymorphism of the STR-locus of Y chromosomes in Eastern Slavs in three populations from Belorussia, Russia and the Ukraine, Genetika 38 (2002) 97–104. [2] P. Lincoln, A. Carracedo, Publication of population data of human polymorphisms, Forensic Sci. Int. 110 (2000) 3–5.

[3] M. Nei, Analysis of gene diversity in subdivided populations, Proc. Natl. Acad. Sci. U.S.A. 70 (1973) 3321–3323. [4] V.L. Pascali, M. Dobosz, B. Brinkmann, Coordinating Y-chromosomal STR research for the courts, Int. J. Leg. Med. 112 (1999) 1. [5] L. Roewer, M. Krawczak, S. Willuweit, M. Nagy, C. Alves, A. Amorim, A. Anslinger, C. Augustin, A. Betz, E. Bosch, A. Caglia´, A. Carracedo, D. Corach, A.F. Dekairelle, T. Dobosz, B.M. Dupuy, S. Fu¨redi, C. Gehrig, L. Gusmao˜, J. Henke, L. Henke, M. Hidding, C. Hohoff, B. Hoste, M.A. Jobling, H.J. Ka¨rgel, P. de Knijff, R. Lessig, E. Liebeherr, M. Lorente, B.

76

R. Lessig et al. / Forensic Science International 159 (2006) 71–76 Martinez-Jarreta, P. Nievas, M. Nowak, W. Parson, V.L. Pascali, G. Penacino, R. Ploski, B. Rolf, A. Sala, U. Schmidt, C. Schmitt, P.M. Schneider, R. Szibor, J. Teifel-Greding, M. Kayser, Online reference database of European Y-chromoso-

mal short tandem repeat (STR) haplotypes, Forensic Sci. Int. 118 (2001) 106–113. [6] F. Tajima, Statistical method for testing the neutral mutation hypothesis by DNA polymorphism, Genetics 123 (1989) 585–595.