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Possibility of genetic-based prognosis of pulmonary tuberculosis in adolescents
S108
5 136
Abstracts
NEW EVIDENCE ON MHC-BASED DISASSORTATIVE ODOR PREFERENCE IN HUMANS: A STUDY WITH 58 BRAZILIAN STUDENTS Pablo C. Santos, Juliano A. Schinemann, Juarez Gabardo, Maria da Grac¸ a Bicalho, Genetics, Universidade Federal do Parana´ , Curitiba, PR, Brazil Increasing evidence suggests correlations between mate choice, odor preferences and the major histocompatibility complex (MHC) in a variety of species. It has been suggested that animals use body odor as a guide to identify possible mates as MHC-similar or dissimilar from their own. Preference for a MHCdissimilar mate is likely to enhance MHC (and overall) heterozygosity of an individual’s offspring. The aim of this work was to search, in humans, new evidence on this subject. Olfactory sessions were performed with 58 students (29 female, 29 male, average age 19.76, ranging from 18 to 27 years), in order to test if preferences for body odors correlate to a person’s HLA phenotype. Odors were collected through sweat and urine from all subjects. Women smelled at and scored all male odor samples (as pleasant, indifferent or unpleasant) and men did the same with female samples. All participants were serologically typed for their HLA-A and -B. Statistical comparisons were performed through Chi-square tests. A significant correlation was found when female smellers evaluated male sweat odors: perceived pleasantness of body odors tend to correlate negatively with the degree of HLA similarity between female smellers and male odor donors. TABLE 1 Number of HLA matches (M) between female smellers and male donors vs female scorings for male sweat samples Matches (M) Pleasant (%) Indifferent (%) Unpleasant (%) 0M 192 (48.24) 82 (20.60) 124 (31.16) 1M 154 (43.63) 90 (25.50) 109 (30.88) 2M 33 (41.25) 30 (37.50) 17 (21.25) 3M 2 (28.57) 4 (57.14) 1 (14.29) Total 381 (45.47) 206 (24.58) 251 (29.25) 2 ⫽ 15.712, d.f. ⫽ 6, P ⫽ 0.0154 Research was funded by the Laboratory of Immunogenetics and Histocompatibility/FUNPAR.
5 137
Total (%) 398 (100) 353 (100) 80 (100) 7 (100) 838 (100)
POSSIBILITY OF GENETIC-BASED PROGNOSIS OF PULMONARY TUBERCULOSIS IN ADOLESCENTS Ludmila N. Bubnova, Irina E. Pavlova, Marina N. Kondakova, Laboratory of Immunohematology, Research Institute of Hematology and transfusiology, St. Petersburg, Russian Federation; Research Institute of Physiopulmonology, St. Petersburg, Russian Federation Identification of genes, determining susceptibility or resistance to tuberculous infection, allows increasing the efficacy of its diagnostics and therapy based on genetic prognosis. HLA II class genes, especially HLADRB1*, are of the most interest for phthisiatry at the moment. The purpose of our study was to evaluate the possibility of genetic prognosis based on the study of HLA-DRB1*-polymorphism in patients with pulmonary tuberculosis. We have studied 50 adolescent patients with PTB, all of whom have undergone full clinic examination at the hospital. HLA-DRB*1 genes were typed by PCR-SSP; control group consisted of 346 healthy residents of North-West region of Russia. We have established that in the group of patients with pulmonary tuberculosis there was decreased frequency of HLA-DRB1*01 allele (10.0% vs. 24.0%, chisquare⫽4,95, p⬍0.05) which can be considered as evidence of protective role of this allele in development of tuberculous infection. The small cohort of patients with HLA-DRB1*01 allele in their phenotype was characterized by favorable course of the disease with marked positive clinical and X-ray dynamics. The obtained data allow us to evaluate the presence of HLA-DRB1*01 allele in patients’ genotype as a protective factor determining individual resistance to pulmonary tuberculosis. In case of disease development in patients with this protective allele the course of tuberculosis is more favourable.
5 136
Abstracts
NEW EVIDENCE ON MHC-BASED DISASSORTATIVE ODOR PREFERENCE IN HUMANS: A STUDY WITH 58 BRAZILIAN STUDENTS Pablo C. Santos, Juliano A. Schinemann, Juarez Gabardo, Maria da Grac¸ a Bicalho, Genetics, Universidade Federal do Parana´ , Curitiba, PR, Brazil Increasing evidence suggests correlations between mate choice, odor preferences and the major histocompatibility complex (MHC) in a variety of species. It has been suggested that animals use body odor as a guide to identify possible mates as MHC-similar or dissimilar from their own. Preference for a MHCdissimilar mate is likely to enhance MHC (and overall) heterozygosity of an individual’s offspring. The aim of this work was to search, in humans, new evidence on this subject. Olfactory sessions were performed with 58 students (29 female, 29 male, average age 19.76, ranging from 18 to 27 years), in order to test if preferences for body odors correlate to a person’s HLA phenotype. Odors were collected through sweat and urine from all subjects. Women smelled at and scored all male odor samples (as pleasant, indifferent or unpleasant) and men did the same with female samples. All participants were serologically typed for their HLA-A and -B. Statistical comparisons were performed through Chi-square tests. A significant correlation was found when female smellers evaluated male sweat odors: perceived pleasantness of body odors tend to correlate negatively with the degree of HLA similarity between female smellers and male odor donors. TABLE 1 Number of HLA matches (M) between female smellers and male donors vs female scorings for male sweat samples Matches (M) Pleasant (%) Indifferent (%) Unpleasant (%) 0M 192 (48.24) 82 (20.60) 124 (31.16) 1M 154 (43.63) 90 (25.50) 109 (30.88) 2M 33 (41.25) 30 (37.50) 17 (21.25) 3M 2 (28.57) 4 (57.14) 1 (14.29) Total 381 (45.47) 206 (24.58) 251 (29.25) 2 ⫽ 15.712, d.f. ⫽ 6, P ⫽ 0.0154 Research was funded by the Laboratory of Immunogenetics and Histocompatibility/FUNPAR.
5 137
Total (%) 398 (100) 353 (100) 80 (100) 7 (100) 838 (100)
POSSIBILITY OF GENETIC-BASED PROGNOSIS OF PULMONARY TUBERCULOSIS IN ADOLESCENTS Ludmila N. Bubnova, Irina E. Pavlova, Marina N. Kondakova, Laboratory of Immunohematology, Research Institute of Hematology and transfusiology, St. Petersburg, Russian Federation; Research Institute of Physiopulmonology, St. Petersburg, Russian Federation Identification of genes, determining susceptibility or resistance to tuberculous infection, allows increasing the efficacy of its diagnostics and therapy based on genetic prognosis. HLA II class genes, especially HLADRB1*, are of the most interest for phthisiatry at the moment. The purpose of our study was to evaluate the possibility of genetic prognosis based on the study of HLA-DRB1*-polymorphism in patients with pulmonary tuberculosis. We have studied 50 adolescent patients with PTB, all of whom have undergone full clinic examination at the hospital. HLA-DRB*1 genes were typed by PCR-SSP; control group consisted of 346 healthy residents of North-West region of Russia. We have established that in the group of patients with pulmonary tuberculosis there was decreased frequency of HLA-DRB1*01 allele (10.0% vs. 24.0%, chisquare⫽4,95, p⬍0.05) which can be considered as evidence of protective role of this allele in development of tuberculous infection. The small cohort of patients with HLA-DRB1*01 allele in their phenotype was characterized by favorable course of the disease with marked positive clinical and X-ray dynamics. The obtained data allow us to evaluate the presence of HLA-DRB1*01 allele in patients’ genotype as a protective factor determining individual resistance to pulmonary tuberculosis. In case of disease development in patients with this protective allele the course of tuberculosis is more favourable.