- Email: [email protected]
Poster 161: Determining the Distribution of Subgingival Microbes of Korean Implant Patients Using 16S rRNA Analysis
Scientific Poster Session
POSTER 160 The Effect of the Depth of the Recipient Site and the Diameter of the Drills on the Implant’s Primary Stability in Pig’s Rib Jae-Young Ryu, DDS 5 Hak-Dong, Dong-Ku, Gwangju, 501-757, Korea (Ryu JY; Lim JS; Youn HJ, Ohk SH; Choi CH; Hong SJ; Kook MS; Park HJ; Oh HK) Statement of the Problem: This study was performed to compare and evaluate the effect of different depths of the recipient site and the diameters of the drills on the primary stability of an implant in a pig’s rib. Materials and Methods: An intact pig’s rib bigger than 8 mm in width and 20 mm in height, and a RBM (Resorbable Blasting Media) surface blasted at ⭋3.75 mm, and 8.0 mm long USII Osstem Implants (Osstem Co., Korea) were used. To measure the primary stability, Periotest (Simens AG, Germany) and Osstell (Model 6 Resonance Frequency Analyser: Integration Diagnostics Ltd., Sweden) were used. The rib was fixed on the vice and then the implants were randomly installed. The formation of the recipient site was done according to the manufacturer’s recommendations: an ⭋1.8 mm guide drill, a ⭋2.0 mm twist drill, a ⭋2.0/⭋3.0 mm pilot drill and a ⭋3.0 mm twist drill were used in that order. Tapping drills and countersink drills were not used. The objects were divided into 6 groups according to the method of formation of its recipient site: D3H3, D3H5, D3H7, D3.3H3, D3.3H5 and D3.3H7. For the D3H3 group (n⫽10), drilling was done using a ⭋1.8 mm guide drill through a ⭋3.0 mm twist drill into 3 mm in depth and then an implant ⭋3.75 mm, 8.0 mm in length was installed with 3mm in depth. The same drilling method was used for the D3H5 (n⫽10) and D3H7 (n⫽10) groups, but the drilling and placement depths were 5 mm and 7 mm, respectively. For the D3.3H3 group (n⫽10), drilling was done using an ⭋1.8 mm guide drill, a ⭋3.0 mm twist drill, a ⭋3.0/⭋3.3 mm pilot drill and a ⭋3.3 mm twist drill in that order. The drilling and placement depth were 3 mm. The same drilling method as in the D3.3H3 group was used for the D3.3H5 (n⫽10) and D3.3H7 (n⫽10) groups with the drilling and placement depths being 5 mm and 7 mm, respectively. The mean value was obtained by taking 4 measurements each on the medio-distal and bucco-lingual side perpendicular to the implant with using Periotest and Osstell. For statistical analysis, one-way ANOVA was used to statistically compare the mean value of each group and the correlations between the placement depths and the primary stability, and that of the measuring instruments were analyzed. Method of Data Analysis: n/a Results: The primary stability of the implants was increased as the placement depths increased (p⬍0.05), and this showed a proportional relationship (p⬍0.01). 43.e92
The primary stability was increased when the diameter of the recipient site was smaller than that of the implant, but this was without a significant difference. There was a strong correlation between Osstell and Periotest, which were used to evaluate the primary stability of the implant (p⬍0.01). Conclusion: These results indicate that increasing the placement depth of implants enhances the primary stability of an implant.
POSTER 161 Determining the Distribution of Subgingival Microbes of Korean Implant Patients Using 16S rRNA Analysis Jae-Young Ryu, DDS, 5 Hak-Dong, Dong-Ku, Gwangju, 501-757, Korea (Ryu JY; Son YW; Youn HJ, Ohk SH; Choi CH; Kook MS; Park HJ; Hong SJ; Oh HK) Statement of the Problem: 16S rRNA analysis is a method of bacterial identification that is useful for identifying bacteria that are otherwise difficult to identify by other means. 12 types of bacteria (A. actinomycetemcomitans, B. forsythensis, C. pneumoniae, E. corrodens, P. gingivalis, P. intermedia, P. nigrescens, S. epidermidis, S. aureus, S. mutans, T. denticola and L. casei) were evaluated in order to study their distribution among Korean patients with dental implants. Materials and Methods: The bacterial distributions of the dental implants and the teeth adjacent to the implants, the teeth opposite the implants and the contralateral teeth were evaluated by the 16S rRNA method. The exudates of the gingival crevice were acquired from those sites in 19 patients with dental implant, and the exudates underwent 16S rRNA synthesis and PCR to identify the microbial flora. Method of Data Analysis: n/a Results: 1. P. gingivalis (50.7%), S. mutans (42.7%) and L. casei (45.3%) were revealed as the most prevalent organisms among the 12 species that were identified. 2. Among the 19 patients, more than 4 species of the tested organisms were detected in 52.6% of the patients and several organisms were detected simultaneously in the adjacent teeth and opposite teeth in 57.9% of the patients. 3. The microbial distribution of the dental implants might be closely related to that of the adjacent teeth. Conclusion: These results suggest that the microbial distribution and oral hygiene of dental implants were closely related to the adjacent teeth. AAOMS • 2007
Scientific Poster Session
POSTER 162 Our Experience With Microtia Reconstruction Using Costocartilage Graft Brian R. Schneider, DDS, MD, 31274 Kenwood, Madison Heights, MI 48071 (Campos-Molina A; Torres I; Gutierrez R) Statement of the Problem: Congenital microtia Materials and Methods: A description of the technique for harvest of costochondral graft and reconstruction of the malformed ear. Method of Data Analysis: Case report Results: Photographs of the reconstruction results in children with congenital microtia Conclusion: The results after ear reconstruction are variable and often disappointing, but with this technique we have found a consistent acceptable ear reconstruction method.
sample, the material was submitted to the hematoxilineosin and Sirius-Red staining colloration. The speciments were analysed by ligth microscopy with a magnification of 400X an 800x. Results: The histological analyses of the graft group showed an integrated graft with the absence of inflammatory cells and approximately half of the bone trabecula showed empty lacuna. New woven bone was observed in areas of bone remodeling. In the Sirius-Red staining on polarized microscopy, new woven bone showed few and unorganized collagen fibers. The histological analyses of the do group showed a partially matured lamellar new bone with intensive proliferation of endosteal osteogenic cells in a well vascularized tissue. The Sirius-Red staining showed a large amount of collagen fibres, organized on the direction of the vector of forces of the lengthening. Conclusion: Although this study has a small group, the histology support some clinical studies reporting that the distraction osteogenesis is superior to the bone graft on the reconstruction of the compromised alveolar maxillary bone.
POSTER 163 Autogenous Bone Graft Versus Distraction Osteogenesis for Maxillary Augmentation an Histological Analysis Marcelo Melo Soares, DDS, PhD, R Guilherme Bannitz 90, CJ 12, Sao Paulo, 04532060, Brazil (Violim L; Guerra F; Marinho L) Statement of the Problem: Since the introduction of osseointegrated implants, the use of autogenous bone graft has been widely reported as the best choice for reconstruction of atrophied alveolar bone. However with the introduction of distraction osteogenesis for alveolar augmentation, several authors have described good results using distraction osteogenesis. The aim of this paper is to compare the use of distraction osteogenesis with autogenous bone graft on the rehabilitation of atrophied anterior maxilla. Materials and Methods: To this study biopsies were collected on two groups of four patients (graft group) submitted to autogenous bone graft (donor site mandibular ramus), and ten patients submitted to distraction osteogenesis (do group). On the graft group the biopsies were collected from patients with successful integration of the graft during the implant installation surgery, showing an average of ten months after the graft procedure Method of Data Analysis: To the do group, the biopsies were collected during the removal of the device and implant installation procedure, showing an average time of 4 months. On all the patients, a control biopsy was collected from the native bone. After the removal of the AAOMS • 2007
References Triplett G, Sterling R. Autogenous bone grafts and endossous implants. Complementary tchniques. J. Oral Maxillofac Surg. 54:48694,1986 Garcia A, Martin M, Vila P, gandara J A preliminary morphologic classification of the alveolar ridge after distraction osteogenesis. J. Oral Maxillofac Surg. 62:563-66,2004
POSTER 164 The New Technique for Harvesting Anterolateral Thigh Flap—Minimizing the Donor Site Morbidity Kazuyoshi Yajima, MD, Hondori 14-chome 1-1, Shiroishi-ku, Sapporo City, Hokkaido, 003-0027, Japan (Ueda M; Rin S; Nakajima Y; Tochihara Y; Yamoto Y; Mol W; Yamashita T) Statement of the Problem: ALT flap has been initially reported as a septo-cutaneous flap, however nowadays, it is known that the intra-muscular type is very common. When harvesting the ALT flap, sometimes a part of the vastus lateralis muscle, rectus femoris muscle and the associated nerves are sacrificed. In this presentation, we are going to demonstrate a harvesting technique to minimize the donor site morbidity using a video presentation. Materials and Methods: From 2005 to 2006, we performed reconstruction in 8 cases of oral cancer using an intramuscular type ALT flap.The important points re43.e93
POSTER 160 The Effect of the Depth of the Recipient Site and the Diameter of the Drills on the Implant’s Primary Stability in Pig’s Rib Jae-Young Ryu, DDS 5 Hak-Dong, Dong-Ku, Gwangju, 501-757, Korea (Ryu JY; Lim JS; Youn HJ, Ohk SH; Choi CH; Hong SJ; Kook MS; Park HJ; Oh HK) Statement of the Problem: This study was performed to compare and evaluate the effect of different depths of the recipient site and the diameters of the drills on the primary stability of an implant in a pig’s rib. Materials and Methods: An intact pig’s rib bigger than 8 mm in width and 20 mm in height, and a RBM (Resorbable Blasting Media) surface blasted at ⭋3.75 mm, and 8.0 mm long USII Osstem Implants (Osstem Co., Korea) were used. To measure the primary stability, Periotest (Simens AG, Germany) and Osstell (Model 6 Resonance Frequency Analyser: Integration Diagnostics Ltd., Sweden) were used. The rib was fixed on the vice and then the implants were randomly installed. The formation of the recipient site was done according to the manufacturer’s recommendations: an ⭋1.8 mm guide drill, a ⭋2.0 mm twist drill, a ⭋2.0/⭋3.0 mm pilot drill and a ⭋3.0 mm twist drill were used in that order. Tapping drills and countersink drills were not used. The objects were divided into 6 groups according to the method of formation of its recipient site: D3H3, D3H5, D3H7, D3.3H3, D3.3H5 and D3.3H7. For the D3H3 group (n⫽10), drilling was done using a ⭋1.8 mm guide drill through a ⭋3.0 mm twist drill into 3 mm in depth and then an implant ⭋3.75 mm, 8.0 mm in length was installed with 3mm in depth. The same drilling method was used for the D3H5 (n⫽10) and D3H7 (n⫽10) groups, but the drilling and placement depths were 5 mm and 7 mm, respectively. For the D3.3H3 group (n⫽10), drilling was done using an ⭋1.8 mm guide drill, a ⭋3.0 mm twist drill, a ⭋3.0/⭋3.3 mm pilot drill and a ⭋3.3 mm twist drill in that order. The drilling and placement depth were 3 mm. The same drilling method as in the D3.3H3 group was used for the D3.3H5 (n⫽10) and D3.3H7 (n⫽10) groups with the drilling and placement depths being 5 mm and 7 mm, respectively. The mean value was obtained by taking 4 measurements each on the medio-distal and bucco-lingual side perpendicular to the implant with using Periotest and Osstell. For statistical analysis, one-way ANOVA was used to statistically compare the mean value of each group and the correlations between the placement depths and the primary stability, and that of the measuring instruments were analyzed. Method of Data Analysis: n/a Results: The primary stability of the implants was increased as the placement depths increased (p⬍0.05), and this showed a proportional relationship (p⬍0.01). 43.e92
The primary stability was increased when the diameter of the recipient site was smaller than that of the implant, but this was without a significant difference. There was a strong correlation between Osstell and Periotest, which were used to evaluate the primary stability of the implant (p⬍0.01). Conclusion: These results indicate that increasing the placement depth of implants enhances the primary stability of an implant.
POSTER 161 Determining the Distribution of Subgingival Microbes of Korean Implant Patients Using 16S rRNA Analysis Jae-Young Ryu, DDS, 5 Hak-Dong, Dong-Ku, Gwangju, 501-757, Korea (Ryu JY; Son YW; Youn HJ, Ohk SH; Choi CH; Kook MS; Park HJ; Hong SJ; Oh HK) Statement of the Problem: 16S rRNA analysis is a method of bacterial identification that is useful for identifying bacteria that are otherwise difficult to identify by other means. 12 types of bacteria (A. actinomycetemcomitans, B. forsythensis, C. pneumoniae, E. corrodens, P. gingivalis, P. intermedia, P. nigrescens, S. epidermidis, S. aureus, S. mutans, T. denticola and L. casei) were evaluated in order to study their distribution among Korean patients with dental implants. Materials and Methods: The bacterial distributions of the dental implants and the teeth adjacent to the implants, the teeth opposite the implants and the contralateral teeth were evaluated by the 16S rRNA method. The exudates of the gingival crevice were acquired from those sites in 19 patients with dental implant, and the exudates underwent 16S rRNA synthesis and PCR to identify the microbial flora. Method of Data Analysis: n/a Results: 1. P. gingivalis (50.7%), S. mutans (42.7%) and L. casei (45.3%) were revealed as the most prevalent organisms among the 12 species that were identified. 2. Among the 19 patients, more than 4 species of the tested organisms were detected in 52.6% of the patients and several organisms were detected simultaneously in the adjacent teeth and opposite teeth in 57.9% of the patients. 3. The microbial distribution of the dental implants might be closely related to that of the adjacent teeth. Conclusion: These results suggest that the microbial distribution and oral hygiene of dental implants were closely related to the adjacent teeth. AAOMS • 2007
Scientific Poster Session
POSTER 162 Our Experience With Microtia Reconstruction Using Costocartilage Graft Brian R. Schneider, DDS, MD, 31274 Kenwood, Madison Heights, MI 48071 (Campos-Molina A; Torres I; Gutierrez R) Statement of the Problem: Congenital microtia Materials and Methods: A description of the technique for harvest of costochondral graft and reconstruction of the malformed ear. Method of Data Analysis: Case report Results: Photographs of the reconstruction results in children with congenital microtia Conclusion: The results after ear reconstruction are variable and often disappointing, but with this technique we have found a consistent acceptable ear reconstruction method.
sample, the material was submitted to the hematoxilineosin and Sirius-Red staining colloration. The speciments were analysed by ligth microscopy with a magnification of 400X an 800x. Results: The histological analyses of the graft group showed an integrated graft with the absence of inflammatory cells and approximately half of the bone trabecula showed empty lacuna. New woven bone was observed in areas of bone remodeling. In the Sirius-Red staining on polarized microscopy, new woven bone showed few and unorganized collagen fibers. The histological analyses of the do group showed a partially matured lamellar new bone with intensive proliferation of endosteal osteogenic cells in a well vascularized tissue. The Sirius-Red staining showed a large amount of collagen fibres, organized on the direction of the vector of forces of the lengthening. Conclusion: Although this study has a small group, the histology support some clinical studies reporting that the distraction osteogenesis is superior to the bone graft on the reconstruction of the compromised alveolar maxillary bone.
POSTER 163 Autogenous Bone Graft Versus Distraction Osteogenesis for Maxillary Augmentation an Histological Analysis Marcelo Melo Soares, DDS, PhD, R Guilherme Bannitz 90, CJ 12, Sao Paulo, 04532060, Brazil (Violim L; Guerra F; Marinho L) Statement of the Problem: Since the introduction of osseointegrated implants, the use of autogenous bone graft has been widely reported as the best choice for reconstruction of atrophied alveolar bone. However with the introduction of distraction osteogenesis for alveolar augmentation, several authors have described good results using distraction osteogenesis. The aim of this paper is to compare the use of distraction osteogenesis with autogenous bone graft on the rehabilitation of atrophied anterior maxilla. Materials and Methods: To this study biopsies were collected on two groups of four patients (graft group) submitted to autogenous bone graft (donor site mandibular ramus), and ten patients submitted to distraction osteogenesis (do group). On the graft group the biopsies were collected from patients with successful integration of the graft during the implant installation surgery, showing an average of ten months after the graft procedure Method of Data Analysis: To the do group, the biopsies were collected during the removal of the device and implant installation procedure, showing an average time of 4 months. On all the patients, a control biopsy was collected from the native bone. After the removal of the AAOMS • 2007
References Triplett G, Sterling R. Autogenous bone grafts and endossous implants. Complementary tchniques. J. Oral Maxillofac Surg. 54:48694,1986 Garcia A, Martin M, Vila P, gandara J A preliminary morphologic classification of the alveolar ridge after distraction osteogenesis. J. Oral Maxillofac Surg. 62:563-66,2004
POSTER 164 The New Technique for Harvesting Anterolateral Thigh Flap—Minimizing the Donor Site Morbidity Kazuyoshi Yajima, MD, Hondori 14-chome 1-1, Shiroishi-ku, Sapporo City, Hokkaido, 003-0027, Japan (Ueda M; Rin S; Nakajima Y; Tochihara Y; Yamoto Y; Mol W; Yamashita T) Statement of the Problem: ALT flap has been initially reported as a septo-cutaneous flap, however nowadays, it is known that the intra-muscular type is very common. When harvesting the ALT flap, sometimes a part of the vastus lateralis muscle, rectus femoris muscle and the associated nerves are sacrificed. In this presentation, we are going to demonstrate a harvesting technique to minimize the donor site morbidity using a video presentation. Materials and Methods: From 2005 to 2006, we performed reconstruction in 8 cases of oral cancer using an intramuscular type ALT flap.The important points re43.e93