- Email: [email protected]
Poster 88
E30
2006 CONGRESS ANNUAL MEETING ABSTRACTS
the week). These included: previsit planning, room standardization, team huddles, designating a clinic flow director, adjusting scheduling time by day of the week, and staggering of patient diagnostic categories. Key Words: Outpatient care; Rehabilitation. Poster 87 Correlation of Electrodiagnostic and Radiologic Severity in S1 Radiculopathy. S. Lee (Dankook University College of Medicine, South Korea), J. Hyun, J. Jeon, K. Kim. Disclosure: None declared. Objective: To investigate the correlation between the severity of electrodiagnostic abnormalities and the magnitude of disk herniation in S1 radiculopathy. Design: Retrospective review of electrodiagnostic and radiologic records. Setting: Outpatient clinic and electrodiagnostic laboratory in university hospital. Participants: 38 patients with definitive symptoms and signs of S1 radiculopathy, who showed electrodiagnostic and radiologic abnormalities. Interventions: Not applicable. Main Outcome Measures: Severity of electrodiagnostic abnormalities was graded according to the severity of each test result, and by the number of tests with abnormal findings. Magnitude of L5-S1 disk herniation was measured from magnetic resonance images. Results: No test results, including needle electromyography, F-wave, H-reflex, and somatosensory-evoked potential studies, showed significant correlation with magnetic resonance images. Number of abnormal test results did not correlate with the size of disk herniation either. Conclusions: Mechanical compression is not the sole determinant of severity of radiculopathy. Key Words: Electrodiagnosis; Radiculopathy; Rehabilitation. Poster 88 Nitric Oxide Mediated Protein Nitration in Experimental Radiculopathies: Inflammatory Versus Compressive. S. Lee (Dankook University College of Medicine, Cheonan, Choongnam, South Korea), J. Hynn, J. Jeon, C. Lee. Disclosure: None declared. Objective: To investigate the role of nitric oxide (NO)⫺ mediated protein nitration in the development of inflammatory and compressive radiculopathies. Design: Randomized controlled trial. Setting: Animal laboratory. Animals: 40 Sprague-Dawley rats randomly assigned to the nucleus pulposus, ligation, sham, or control groups. Interventions: Radiculopathy was induced by grafting autologous nucleus pulposus (NP group) or by ligation of nerve roots with silk (ligation group). Main Outcome Measures: Mechanical allodynia was determined by measuring the number of withdrawal responses from plantar stimulation, using calibrated nylon filaments. Motor weakness was evaluated and graded by observing the gait pattern. Nitrotyrosine content was assayed by Western blotting and immunohistochemical staining. Results: The NP and ligation groups showed increased mechanical allodynia postoperatively compared with the sham and control groups. Motor weakness was evident only in the ligation group. Western blotting demonstrated increased optical densities of nitrotyrosine bands in the NP and ligation groups, and immunoreactivities for nitrotyrosine were demonstrated in immunohistochemical staining. Conclusions: NO-mediated protein nitration should be among the athophysiologic mechanisms in compressive radiculopathy, as well as in inflammatory radiculopathy. Inhibition of NO overproduction may be helpful in rehabilitation of patients with radicular pain. Key Words: Nitric oxide; Radiculopathy; Rehabilitation. Arch Phys Med Rehabil Vol 87, October 2006
Poster 89: Cancelled. Poster 90 Expression of Muscular Growth Factor on Passive Stretching in the Rat Skeletal Muscle. S. Ikeda (Kagoshima University, Kogashima, Japan), K. Harada, Y. Kamikawa, A. Yoshida, Y. Nomoto, A. Oowatashi, K. Kawahira. Disclosure: None declared. Objective: To investigate the effect of muscle growth by passive stretching exercise on the rat skeletal muscle. Design: Case-control study. Setting: University laboratory. Animals: 10 male Wistar rats weighing 250 to 350g. Intervention: Continuous passive stretching of unilateral gastrocnemius muscle under anesthesia by ankle dorsiflexion for 15 minutes a day, and 4 procedures were done within the week. Then muscles were removed and ribonucleic acid (RNA) was extracted. Main Outcome Measure: Semiquantitation of the messenger RNA was done for the mechanogrowth factor, that is, an autocrine variant of insulin-like growth factor secreted from muscle, and myogenin, a myogenic transcription factor that enhances the gene expression of the muscle, using the reverse transcription polymerase chain reaction method. Results: Messenger RNA of the mechanogrowth factor was significantly increased in stretched gastrocnemius muscle as compared with the unstretched contralateral one. The expression of myogenin messenger RNA trend to increase, however, the difference was not significant. Conclusions: Brief passive stretching exercise induces the mechanogrowth factor. This finding suggests that passive stretching may prevent the disuse atrophy of patients in an unconscious state or with neurologic defects. Key Words: Reflex, stretch; Rehabilitation. Poster 91 Functional Recovery From Second Contralateral Infarction on the Rat Hemiplegic Model. K. Harada (Kagoshima University, Kogashima, Japan), S. Ikeda, Y. Kamikawa, A. Yoshida, Y. Nomoto, A. Oowatashi, K. Kawahira Disclosure: None declared. Objective: To investigate functional recovery after contralateral secondary infarction, using photochemically induced rat cerebral infarction model, in order to clarify the role of contralateral cerebral cortex in the rat hemiplegia model. Design: Case series. Setting: University laboratory. Animals: 8 Wistar rats at age 7 weeks. Interventions: Under irradiation of green light wavelength at 560nm on the sensorimotor area of right hemisphere, photo-sensitive rose bengal dye was injected intravenously to make first infarction. 2 weeks later, a secondary infarction was made in the left cerebral cortex after recovery from first hemiplegia. Main Outcome Measures: The beam walking test of Feeney et al and evaluation of functional recovery from hemiplegia. Result: 1 day after infarction, hemiplegia was observed; the score was 1 on both the first and second infarctions. The average recovery score was 6.8⫾0.1 by day 14 after the first infarction. However, after the second infarction, functional recovery was slow and reached a score of 4.2⫾0.5 at 14 days postinfarction. Conclusions: Our results suggest that contralateral cortex has an important role in the recovery process in hemiplegia after cerebral infarction. Key Words: Cerebral infarction; Recovery of function; Rehabilitation. Poster 92 Effects of Water Currents on the Muscular Activation of Certain Trunk Stabilizing Muscles. J.C. Colado (Catholic University of Murcia, Mutxamel, Alicante, Spain), V. Tella. Disclosure: None declared.
2006 CONGRESS ANNUAL MEETING ABSTRACTS
the week). These included: previsit planning, room standardization, team huddles, designating a clinic flow director, adjusting scheduling time by day of the week, and staggering of patient diagnostic categories. Key Words: Outpatient care; Rehabilitation. Poster 87 Correlation of Electrodiagnostic and Radiologic Severity in S1 Radiculopathy. S. Lee (Dankook University College of Medicine, South Korea), J. Hyun, J. Jeon, K. Kim. Disclosure: None declared. Objective: To investigate the correlation between the severity of electrodiagnostic abnormalities and the magnitude of disk herniation in S1 radiculopathy. Design: Retrospective review of electrodiagnostic and radiologic records. Setting: Outpatient clinic and electrodiagnostic laboratory in university hospital. Participants: 38 patients with definitive symptoms and signs of S1 radiculopathy, who showed electrodiagnostic and radiologic abnormalities. Interventions: Not applicable. Main Outcome Measures: Severity of electrodiagnostic abnormalities was graded according to the severity of each test result, and by the number of tests with abnormal findings. Magnitude of L5-S1 disk herniation was measured from magnetic resonance images. Results: No test results, including needle electromyography, F-wave, H-reflex, and somatosensory-evoked potential studies, showed significant correlation with magnetic resonance images. Number of abnormal test results did not correlate with the size of disk herniation either. Conclusions: Mechanical compression is not the sole determinant of severity of radiculopathy. Key Words: Electrodiagnosis; Radiculopathy; Rehabilitation. Poster 88 Nitric Oxide Mediated Protein Nitration in Experimental Radiculopathies: Inflammatory Versus Compressive. S. Lee (Dankook University College of Medicine, Cheonan, Choongnam, South Korea), J. Hynn, J. Jeon, C. Lee. Disclosure: None declared. Objective: To investigate the role of nitric oxide (NO)⫺ mediated protein nitration in the development of inflammatory and compressive radiculopathies. Design: Randomized controlled trial. Setting: Animal laboratory. Animals: 40 Sprague-Dawley rats randomly assigned to the nucleus pulposus, ligation, sham, or control groups. Interventions: Radiculopathy was induced by grafting autologous nucleus pulposus (NP group) or by ligation of nerve roots with silk (ligation group). Main Outcome Measures: Mechanical allodynia was determined by measuring the number of withdrawal responses from plantar stimulation, using calibrated nylon filaments. Motor weakness was evaluated and graded by observing the gait pattern. Nitrotyrosine content was assayed by Western blotting and immunohistochemical staining. Results: The NP and ligation groups showed increased mechanical allodynia postoperatively compared with the sham and control groups. Motor weakness was evident only in the ligation group. Western blotting demonstrated increased optical densities of nitrotyrosine bands in the NP and ligation groups, and immunoreactivities for nitrotyrosine were demonstrated in immunohistochemical staining. Conclusions: NO-mediated protein nitration should be among the athophysiologic mechanisms in compressive radiculopathy, as well as in inflammatory radiculopathy. Inhibition of NO overproduction may be helpful in rehabilitation of patients with radicular pain. Key Words: Nitric oxide; Radiculopathy; Rehabilitation. Arch Phys Med Rehabil Vol 87, October 2006
Poster 89: Cancelled. Poster 90 Expression of Muscular Growth Factor on Passive Stretching in the Rat Skeletal Muscle. S. Ikeda (Kagoshima University, Kogashima, Japan), K. Harada, Y. Kamikawa, A. Yoshida, Y. Nomoto, A. Oowatashi, K. Kawahira. Disclosure: None declared. Objective: To investigate the effect of muscle growth by passive stretching exercise on the rat skeletal muscle. Design: Case-control study. Setting: University laboratory. Animals: 10 male Wistar rats weighing 250 to 350g. Intervention: Continuous passive stretching of unilateral gastrocnemius muscle under anesthesia by ankle dorsiflexion for 15 minutes a day, and 4 procedures were done within the week. Then muscles were removed and ribonucleic acid (RNA) was extracted. Main Outcome Measure: Semiquantitation of the messenger RNA was done for the mechanogrowth factor, that is, an autocrine variant of insulin-like growth factor secreted from muscle, and myogenin, a myogenic transcription factor that enhances the gene expression of the muscle, using the reverse transcription polymerase chain reaction method. Results: Messenger RNA of the mechanogrowth factor was significantly increased in stretched gastrocnemius muscle as compared with the unstretched contralateral one. The expression of myogenin messenger RNA trend to increase, however, the difference was not significant. Conclusions: Brief passive stretching exercise induces the mechanogrowth factor. This finding suggests that passive stretching may prevent the disuse atrophy of patients in an unconscious state or with neurologic defects. Key Words: Reflex, stretch; Rehabilitation. Poster 91 Functional Recovery From Second Contralateral Infarction on the Rat Hemiplegic Model. K. Harada (Kagoshima University, Kogashima, Japan), S. Ikeda, Y. Kamikawa, A. Yoshida, Y. Nomoto, A. Oowatashi, K. Kawahira Disclosure: None declared. Objective: To investigate functional recovery after contralateral secondary infarction, using photochemically induced rat cerebral infarction model, in order to clarify the role of contralateral cerebral cortex in the rat hemiplegia model. Design: Case series. Setting: University laboratory. Animals: 8 Wistar rats at age 7 weeks. Interventions: Under irradiation of green light wavelength at 560nm on the sensorimotor area of right hemisphere, photo-sensitive rose bengal dye was injected intravenously to make first infarction. 2 weeks later, a secondary infarction was made in the left cerebral cortex after recovery from first hemiplegia. Main Outcome Measures: The beam walking test of Feeney et al and evaluation of functional recovery from hemiplegia. Result: 1 day after infarction, hemiplegia was observed; the score was 1 on both the first and second infarctions. The average recovery score was 6.8⫾0.1 by day 14 after the first infarction. However, after the second infarction, functional recovery was slow and reached a score of 4.2⫾0.5 at 14 days postinfarction. Conclusions: Our results suggest that contralateral cortex has an important role in the recovery process in hemiplegia after cerebral infarction. Key Words: Cerebral infarction; Recovery of function; Rehabilitation. Poster 92 Effects of Water Currents on the Muscular Activation of Certain Trunk Stabilizing Muscles. J.C. Colado (Catholic University of Murcia, Mutxamel, Alicante, Spain), V. Tella. Disclosure: None declared.