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Potamogeton nodosus: an aquatic source for bioactive compounds
Fitoterapia 70 Ž1999. 523]525
Short report
Potamogeton nodosus: an aquatic source for bioactive compounds Zenefar Alam, M. Mostaqul Huq, A. Jabbar, C.M. HasanU Department of Pharmacy, Faculty of Pharmacy, Uni¨ ersity of Dhaka, Dhaka-1000, Bangladesh
Received 8 March 1999; accepted 25 March 1999
Abstract 2-Hydroxyheptane-3,5-dione Ž1. was obtained from the petrol-soluble fraction ŽPE. of the ethanol extract of Potamogeton nodosus. Biological screening of PE revealed antibacterial activity and cytotoxicity. Compound 1 showed only antibacterial activity. Q 1998 Elsevier Science B.V. All rights reserved. Keywords: Potamogeton nodosus; 2-Hydroxyheptane-3,5-dione; Aliphatics; Antibacterial activity; Cytotoxicity
Plant. Potamogeton nodosus Poir. ŽPotamogetonaceae., fresh submerged aquatic herb, collected from a dead river of Chuadanga District, Bangladesh, in April 1997 and identified by Bangladesh National Herbarium, Dhaka. Uses in traditional medicine. The plant has been reported to be active against cancer, tuberculosis, acne, common cough and cold, wounds and abdominal discomfort w1x. U
Corresponding author. Tel.: q880-2-505420; fax: q880-2-865583 E-mail address: [email protected] ŽC.M. Hasan.
0367-326Xr98r$ - see front matter Q 1998 Elsevier Science B.V. All rights reserved. PII: S 0 3 6 7 - 3 2 6 X Ž 9 9 . 0 0 0 8 0 - 5
Z. Alam et al. r Fitoterapia 70 (1999) 523]525
524
Table 1 In vitro antibacterial activity of extractives from Potamogeton nodosusa Microorganisms
Diameter of zone of inhibition Žmm. PE Ž250 mgrdisc.
1 Ž200 mgrdisc.
Kanamycin Ž30 mgrdisc.
Gram-positive Bacillus megaterium QL38 Bacillus cereus QL29 Bacillus subtilis QL40 Sarcina lutea QL166 Staphylococcus aureus ATCC25923
12 7 13 ] 10
14 nd nd nd 12
32 29 30 31 30
Gram-negative Escherichia coli FPFC281 Klebsiella sp. Pseudomonas aeruginosa CRL Shigella flexneri AL30372 Shigella boydii AL17313 Shigella sonnei AJ8992 Salmonella paratyphi-B AG1407 Vibrio mimicus CRL Vibrio parahaemophylia CRL Vibrio cholerae AD66
12 10 ] ] 12 8 10 8 11 ]
12 ] nd nd 10 ] 9 7 nd nd
29 35 34 27 29 25 30 29 30 32
a
], No inhibition; nd, not done due scarcity of sample; PE, petrol-soluble fraction of ethanol extract; 1, 2-hydroxyheptane-3,5-dione.
Previously isolated constituents. An antibacterial furanoid diterpene, 15,16-epoxy12-oxo-8Ž17.,13Ž16.,14-labdatrien-19,20-olide w2x. New-isolated constituent. 2-Hydroxyheptane-3,5-dione Ž1., yield 0.01%, as colorless, fragrant oil, IR bands ŽKBr.: 3450, 2900, 1710, 1410, 1260, 1150 cmy1 ; 1 H-NMR Ž500 MHz, CDCl 3 .: d 4.12 Ž1H, q, J 7 Hz, H-2., 3.66 Ž2H, s, H-4., 2.31 Ž2H, q, J 7.32 Hz, H-6., 1.6 Ž3H, d, J 7 Hz, H-1., 0.88 Ž3H, t, J 7.32 Hz, H-7.. Tested material. Petrol-soluble fraction of the ethanol extract and therefrom isolated compound 1. Studied activity. Antibacterial activity by disc diffusion method w3x and cytotoxicity by brine shrimp lethality bioassay w4,5x. Used microorganisms. The test microorganisms Žsee Table 1. were obtained from the Institute of Nutrition and Food Science ŽINFS., University of Dhaka, Bangladesh. Results. Reported in Table 1 Žantibacterial activity.. The LC 50 Žmedian lethal concentration . of the brine shrimp lethality bioassay was 35.36 and 150 mgrml for PE and compound 1, respectively.
Z. Alam et al. r Fitoterapia 70 (1999) 523]525
525
Conclusions. Both PE and compound 1 showed antibacterial activity against most test microorganisms. PE exhibited also moderate cytotoxicity, not observed with compound 1. The obtained results may provide a support to some uses of the plants in traditional medicine.
Acknowledgements The authors wish to thank Md. Shawkat Ali, on Ph.D. study in Japan, for recording the NMR spectrum of the compound.
References w1x Bisaws SK, Ghosh SE. Bharatio Banaushadhi, vol. II, 2nd ed. Calcutta: Calcutta University Press, 1977:1457]1459. w2x Qais N, Mandal MR, Rashid MA et al. J Nat Prod 1998;61:156. w3x Baurer AW, Kirby WMM, Sherris JC, Truck M. Am J Clin Pathol 1966;45:493. w4x Meyer BN, Ferrigni NR, Jacobsen LB, Nicholos DE, Mclaughlin JL. Planta Med 1982;45:31. w5x Goldstein AL, Arnold L, Kalkan SM. Principles of drug action. 2nd ed. Wiley Biomedical Publication, 1974:376]381.
Short report
Potamogeton nodosus: an aquatic source for bioactive compounds Zenefar Alam, M. Mostaqul Huq, A. Jabbar, C.M. HasanU Department of Pharmacy, Faculty of Pharmacy, Uni¨ ersity of Dhaka, Dhaka-1000, Bangladesh
Received 8 March 1999; accepted 25 March 1999
Abstract 2-Hydroxyheptane-3,5-dione Ž1. was obtained from the petrol-soluble fraction ŽPE. of the ethanol extract of Potamogeton nodosus. Biological screening of PE revealed antibacterial activity and cytotoxicity. Compound 1 showed only antibacterial activity. Q 1998 Elsevier Science B.V. All rights reserved. Keywords: Potamogeton nodosus; 2-Hydroxyheptane-3,5-dione; Aliphatics; Antibacterial activity; Cytotoxicity
Plant. Potamogeton nodosus Poir. ŽPotamogetonaceae., fresh submerged aquatic herb, collected from a dead river of Chuadanga District, Bangladesh, in April 1997 and identified by Bangladesh National Herbarium, Dhaka. Uses in traditional medicine. The plant has been reported to be active against cancer, tuberculosis, acne, common cough and cold, wounds and abdominal discomfort w1x. U
Corresponding author. Tel.: q880-2-505420; fax: q880-2-865583 E-mail address: [email protected] ŽC.M. Hasan.
0367-326Xr98r$ - see front matter Q 1998 Elsevier Science B.V. All rights reserved. PII: S 0 3 6 7 - 3 2 6 X Ž 9 9 . 0 0 0 8 0 - 5
Z. Alam et al. r Fitoterapia 70 (1999) 523]525
524
Table 1 In vitro antibacterial activity of extractives from Potamogeton nodosusa Microorganisms
Diameter of zone of inhibition Žmm. PE Ž250 mgrdisc.
1 Ž200 mgrdisc.
Kanamycin Ž30 mgrdisc.
Gram-positive Bacillus megaterium QL38 Bacillus cereus QL29 Bacillus subtilis QL40 Sarcina lutea QL166 Staphylococcus aureus ATCC25923
12 7 13 ] 10
14 nd nd nd 12
32 29 30 31 30
Gram-negative Escherichia coli FPFC281 Klebsiella sp. Pseudomonas aeruginosa CRL Shigella flexneri AL30372 Shigella boydii AL17313 Shigella sonnei AJ8992 Salmonella paratyphi-B AG1407 Vibrio mimicus CRL Vibrio parahaemophylia CRL Vibrio cholerae AD66
12 10 ] ] 12 8 10 8 11 ]
12 ] nd nd 10 ] 9 7 nd nd
29 35 34 27 29 25 30 29 30 32
a
], No inhibition; nd, not done due scarcity of sample; PE, petrol-soluble fraction of ethanol extract; 1, 2-hydroxyheptane-3,5-dione.
Previously isolated constituents. An antibacterial furanoid diterpene, 15,16-epoxy12-oxo-8Ž17.,13Ž16.,14-labdatrien-19,20-olide w2x. New-isolated constituent. 2-Hydroxyheptane-3,5-dione Ž1., yield 0.01%, as colorless, fragrant oil, IR bands ŽKBr.: 3450, 2900, 1710, 1410, 1260, 1150 cmy1 ; 1 H-NMR Ž500 MHz, CDCl 3 .: d 4.12 Ž1H, q, J 7 Hz, H-2., 3.66 Ž2H, s, H-4., 2.31 Ž2H, q, J 7.32 Hz, H-6., 1.6 Ž3H, d, J 7 Hz, H-1., 0.88 Ž3H, t, J 7.32 Hz, H-7.. Tested material. Petrol-soluble fraction of the ethanol extract and therefrom isolated compound 1. Studied activity. Antibacterial activity by disc diffusion method w3x and cytotoxicity by brine shrimp lethality bioassay w4,5x. Used microorganisms. The test microorganisms Žsee Table 1. were obtained from the Institute of Nutrition and Food Science ŽINFS., University of Dhaka, Bangladesh. Results. Reported in Table 1 Žantibacterial activity.. The LC 50 Žmedian lethal concentration . of the brine shrimp lethality bioassay was 35.36 and 150 mgrml for PE and compound 1, respectively.
Z. Alam et al. r Fitoterapia 70 (1999) 523]525
525
Conclusions. Both PE and compound 1 showed antibacterial activity against most test microorganisms. PE exhibited also moderate cytotoxicity, not observed with compound 1. The obtained results may provide a support to some uses of the plants in traditional medicine.
Acknowledgements The authors wish to thank Md. Shawkat Ali, on Ph.D. study in Japan, for recording the NMR spectrum of the compound.
References w1x Bisaws SK, Ghosh SE. Bharatio Banaushadhi, vol. II, 2nd ed. Calcutta: Calcutta University Press, 1977:1457]1459. w2x Qais N, Mandal MR, Rashid MA et al. J Nat Prod 1998;61:156. w3x Baurer AW, Kirby WMM, Sherris JC, Truck M. Am J Clin Pathol 1966;45:493. w4x Meyer BN, Ferrigni NR, Jacobsen LB, Nicholos DE, Mclaughlin JL. Planta Med 1982;45:31. w5x Goldstein AL, Arnold L, Kalkan SM. Principles of drug action. 2nd ed. Wiley Biomedical Publication, 1974:376]381.