- Email: [email protected]
warmed oocytes
S16
Abstracts of the 11th Biennial Conference of Alpha, Scientists in Reproductive Medicine
University Hospital, Aalborg, Denmark) between June 2014 and December 2015. The samples were assessed using the SCA® (Sperm Class Analyzer) CASA system. Results demonstrated a lower ejaculation volume with significantly different motion- path parameters (LIN: linearity, STR: straightness) and significantly higher velocity (VSL, VAP and VCL) parameters in sperm collected after only 2 hours compared to 4–7 days of sexual abstinence. Shorter abstinence also resulted in a significantly higher percentage of progressive motile sperm. These findings challenge the usefulness of the WHO reference values suggesting 2–7 days of abstinence in male-factor infertility treatments. Thus this study suggests a much shorter abstinence time, as low as 2 hours, in order to present the best possible semen quality for use in assisted reproductive technologies. Keywords: Sperm quality, Sexual abstinence, Consecutive ejacu lation, Computer assisted sperm analysis, Sperm class analyzer, SCA
PP-032 [Abstract:0060][Reviewed][Embryology]
Which is the optimal time of intracytoplasmic sperm injection (ICSI) after vitrified/warmed oocytes Bruna C Barros1, Raquel Mazetto1, Thais S Domingues2, Paulo C Serafini2, Eduardo L A Motta2, Jose Roberto Alegretti1 1 Embriology - Huntington Medicina Reprodutiva, São Paulo, Brazil; 2Clinical - Huntington Medicina Reprodutiva, São Paulo, Brazil Background: Vitrification produces glasslike solidification of living cells that completely avoids ice crystal formation during cooling and warming, widely used in oocyte cryopreservation for in-vitro fertilization (IVF). The aim of this study was to compare different times of sperm injection after vitrified/warmed oocytes and their development up to blastocysts. Materials and Methods: This prospective study included 50 cycles of vitrified/warmed oocyte using an open system protocol. For every cycle, a cohort of 6 to 12 oocytes were warmed and subdivided into two groups according to the ICSI time: A) ICSI performed 1:30 hours after oocytes warming. B) ICSI performed 2:00 hours after oocytes warming. All embryos were cultured until blastocyst stage and evaluated at day 1 (pronuclear stage), day 3 (cleavage grade) and day 5/6 (blastocyst formation and grade). The final analysis compared the top quality blastocysts rate per D3 cleaved embryos, between groups A and B using paired t Test. Significance was reached at p<0.05. Results: A total of 869 oocytes vitrified/warmed were fertilized by ICSI, 438 oocytes (group A) and 431 (group B). The mean number of oocytes/cycle were A: 4.6±0.12 and B: 4.1±0.12. The fertilization rate (A: 77.6% and B: 78.3%; p=0.871), and rate of blastocyst formation (A: 55.3% and B: 47.2%; p=0.267) were similar between groups. However, the top quality blastocyst according to Gardner grading (expansion 3/4 and grade A/B or expansion 5/6) was higher when the oocytes were injected 1:30 after warming (A: 38.6%) compared to those injected at 2:00 after warming (25.3%; p=0.034). Blastocysts were transferred according to morphological classification, independent of ICSI time. The overall clinical pregnancy rate was 58.7%. Conclusion: Vitrified oocytes manipulated by ICSI at 1:30 or 2:00h after warming showed no differences on fertilization rate and blastocyst formation. However top quality blastocyst rate was higher when the oocytes were injected earlier, after 1:30 hours post warming. Our findings suggest that 1:30 hour is enough for the structural reconfiguration of meiotical spindle, allowing the optimal time variation to reach better outcomes. This study was not designed to identify the oocyte competence after vitrification/ warming process, and further studies will be developed. Keywords: Oocyte vitrification, ICSI, Blastocyst
PP-033 [Abstract:0061][Reviewed][Andrology]
Lactobacillus rhamnosus PB01 can increase the serum total antioxidant capacity suggesting a potential positive effect on sperm motility Fereshteh Dardmeh1, Hiva Alipour1, Parisa Gazerani2, Sigríður Olga Magnúsdóttir3, Erik Brandsborg4, Hans Ingolf Nielsen1 1 Biomedicine Group, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark; 2SMI®, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark; 3 Department of clinical medicine, The faculty of Medicine, Aalborg University Hospital, Aalborg, Denmark; 4Bifodan A/S, Hundested, Denmark Disruption of the balance between oxidative and anti-oxidative processes has repeatedly been reported to affect sperm quality. Obesity as a medical condition has also been correlated with an overall poor semen quality and sub-fecundity. Antioxidant properties of some probiotics have been previously demonstrated. This study investigated the antioxidant capability and effect of probiotic Lactobacillus rhamnosus PB01 on sperm motility in dietinduced obese mice. Twenty-four 6 weeks old C57BL/6NTac mice were placed on either a normal or high-fat diet for 4 weeks to create the normal control or diet-induced obesity (DIO) models, respectively. The DIO and control groups were randomly sub divided into two groups and maintained on their respective diet with or without a single daily dose (1×109 CFU) of Lactobacillus rhamnosus PB01 (DSM 14870) (Bifodan A/S, Denmark) for another 4 weeks. At the end of the experimental period, the serum total antioxidant capacity (TAC) was assessed by the enzyme-linked immunosorbent assay (ELISA) and correlated with the sperm motility and kinematic parameters of semen samples analyzed by the Sperm Class Analyzer (Microptic S.L., Spain). The diet-induced obesity group demonstrated a reduced serum TAC, while probiotic supplementation was found to increase TAC in both DIO and normal control groups. The DIO group also demonstrated a clear reduction in several kinematic parameters (VCL, VSL, VAP, STR and LIN) including the percentage of progressive motile sperm. However, these alterations were reversed proportionally in the DIO-probiotics-supplemented group. It is therefore proposed that the applied probiotic might exert a protective role on sperm quality including sperm motility through mechanisms associated with its antioxidant capacity. The present results indicated that Lactobacillus rhamnosus PB01 could enhance serum TAC, which eventually plays a protective role against sperm damage under high-fat diet condition. This study supports the hypothesis of a probiotics’ positive effect on sperm motility and overall quality that can be tailored for future tests in obese sub-fertile men. Keywords: Total antioxidant capacity, Sperm quality, Sperm kinematics, Probiotics, Lactobacillus rhamnosus,
PP-034 [Abstract:0063][Reviewed][Genetics]
Case report: PGS can increase success in patients undergoing IVF-PGD treatment for Fragile-X disorder Carmen Miralles Cuadrado1, Alex Page1, Alison Campbell2 CARE London, London, UK; 2CARE Corporate, Manchester, UK
1
Introduction: Fragile-X associated disorders are a group of conditions referred to as trinucleotide repeat disorders. PGD is a technique recommended for carriers of a Fragile-X permutation that wish to have unaffected offspring. PGS is a technique used for the screening of chromosomes of an embryo, with the aim of identifying euploid embryos.
Abstracts of the 11th Biennial Conference of Alpha, Scientists in Reproductive Medicine
University Hospital, Aalborg, Denmark) between June 2014 and December 2015. The samples were assessed using the SCA® (Sperm Class Analyzer) CASA system. Results demonstrated a lower ejaculation volume with significantly different motion- path parameters (LIN: linearity, STR: straightness) and significantly higher velocity (VSL, VAP and VCL) parameters in sperm collected after only 2 hours compared to 4–7 days of sexual abstinence. Shorter abstinence also resulted in a significantly higher percentage of progressive motile sperm. These findings challenge the usefulness of the WHO reference values suggesting 2–7 days of abstinence in male-factor infertility treatments. Thus this study suggests a much shorter abstinence time, as low as 2 hours, in order to present the best possible semen quality for use in assisted reproductive technologies. Keywords: Sperm quality, Sexual abstinence, Consecutive ejacu lation, Computer assisted sperm analysis, Sperm class analyzer, SCA
PP-032 [Abstract:0060][Reviewed][Embryology]
Which is the optimal time of intracytoplasmic sperm injection (ICSI) after vitrified/warmed oocytes Bruna C Barros1, Raquel Mazetto1, Thais S Domingues2, Paulo C Serafini2, Eduardo L A Motta2, Jose Roberto Alegretti1 1 Embriology - Huntington Medicina Reprodutiva, São Paulo, Brazil; 2Clinical - Huntington Medicina Reprodutiva, São Paulo, Brazil Background: Vitrification produces glasslike solidification of living cells that completely avoids ice crystal formation during cooling and warming, widely used in oocyte cryopreservation for in-vitro fertilization (IVF). The aim of this study was to compare different times of sperm injection after vitrified/warmed oocytes and their development up to blastocysts. Materials and Methods: This prospective study included 50 cycles of vitrified/warmed oocyte using an open system protocol. For every cycle, a cohort of 6 to 12 oocytes were warmed and subdivided into two groups according to the ICSI time: A) ICSI performed 1:30 hours after oocytes warming. B) ICSI performed 2:00 hours after oocytes warming. All embryos were cultured until blastocyst stage and evaluated at day 1 (pronuclear stage), day 3 (cleavage grade) and day 5/6 (blastocyst formation and grade). The final analysis compared the top quality blastocysts rate per D3 cleaved embryos, between groups A and B using paired t Test. Significance was reached at p<0.05. Results: A total of 869 oocytes vitrified/warmed were fertilized by ICSI, 438 oocytes (group A) and 431 (group B). The mean number of oocytes/cycle were A: 4.6±0.12 and B: 4.1±0.12. The fertilization rate (A: 77.6% and B: 78.3%; p=0.871), and rate of blastocyst formation (A: 55.3% and B: 47.2%; p=0.267) were similar between groups. However, the top quality blastocyst according to Gardner grading (expansion 3/4 and grade A/B or expansion 5/6) was higher when the oocytes were injected 1:30 after warming (A: 38.6%) compared to those injected at 2:00 after warming (25.3%; p=0.034). Blastocysts were transferred according to morphological classification, independent of ICSI time. The overall clinical pregnancy rate was 58.7%. Conclusion: Vitrified oocytes manipulated by ICSI at 1:30 or 2:00h after warming showed no differences on fertilization rate and blastocyst formation. However top quality blastocyst rate was higher when the oocytes were injected earlier, after 1:30 hours post warming. Our findings suggest that 1:30 hour is enough for the structural reconfiguration of meiotical spindle, allowing the optimal time variation to reach better outcomes. This study was not designed to identify the oocyte competence after vitrification/ warming process, and further studies will be developed. Keywords: Oocyte vitrification, ICSI, Blastocyst
PP-033 [Abstract:0061][Reviewed][Andrology]
Lactobacillus rhamnosus PB01 can increase the serum total antioxidant capacity suggesting a potential positive effect on sperm motility Fereshteh Dardmeh1, Hiva Alipour1, Parisa Gazerani2, Sigríður Olga Magnúsdóttir3, Erik Brandsborg4, Hans Ingolf Nielsen1 1 Biomedicine Group, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark; 2SMI®, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark; 3 Department of clinical medicine, The faculty of Medicine, Aalborg University Hospital, Aalborg, Denmark; 4Bifodan A/S, Hundested, Denmark Disruption of the balance between oxidative and anti-oxidative processes has repeatedly been reported to affect sperm quality. Obesity as a medical condition has also been correlated with an overall poor semen quality and sub-fecundity. Antioxidant properties of some probiotics have been previously demonstrated. This study investigated the antioxidant capability and effect of probiotic Lactobacillus rhamnosus PB01 on sperm motility in dietinduced obese mice. Twenty-four 6 weeks old C57BL/6NTac mice were placed on either a normal or high-fat diet for 4 weeks to create the normal control or diet-induced obesity (DIO) models, respectively. The DIO and control groups were randomly sub divided into two groups and maintained on their respective diet with or without a single daily dose (1×109 CFU) of Lactobacillus rhamnosus PB01 (DSM 14870) (Bifodan A/S, Denmark) for another 4 weeks. At the end of the experimental period, the serum total antioxidant capacity (TAC) was assessed by the enzyme-linked immunosorbent assay (ELISA) and correlated with the sperm motility and kinematic parameters of semen samples analyzed by the Sperm Class Analyzer (Microptic S.L., Spain). The diet-induced obesity group demonstrated a reduced serum TAC, while probiotic supplementation was found to increase TAC in both DIO and normal control groups. The DIO group also demonstrated a clear reduction in several kinematic parameters (VCL, VSL, VAP, STR and LIN) including the percentage of progressive motile sperm. However, these alterations were reversed proportionally in the DIO-probiotics-supplemented group. It is therefore proposed that the applied probiotic might exert a protective role on sperm quality including sperm motility through mechanisms associated with its antioxidant capacity. The present results indicated that Lactobacillus rhamnosus PB01 could enhance serum TAC, which eventually plays a protective role against sperm damage under high-fat diet condition. This study supports the hypothesis of a probiotics’ positive effect on sperm motility and overall quality that can be tailored for future tests in obese sub-fertile men. Keywords: Total antioxidant capacity, Sperm quality, Sperm kinematics, Probiotics, Lactobacillus rhamnosus,
PP-034 [Abstract:0063][Reviewed][Genetics]
Case report: PGS can increase success in patients undergoing IVF-PGD treatment for Fragile-X disorder Carmen Miralles Cuadrado1, Alex Page1, Alison Campbell2 CARE London, London, UK; 2CARE Corporate, Manchester, UK
1
Introduction: Fragile-X associated disorders are a group of conditions referred to as trinucleotide repeat disorders. PGD is a technique recommended for carriers of a Fragile-X permutation that wish to have unaffected offspring. PGS is a technique used for the screening of chromosomes of an embryo, with the aim of identifying euploid embryos.