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PP-42 THE NEW CONTACTLESS METHOD FOR TROPHECTODERM BIOPSY
Posters PP-42 THE NEW CONTACTLESS METHOD FOR TROPHECTODERM BIOPSY Yulia Khramova1 , Sergey Sergeev1 , Inna Ilina2 , Dmitry Sitnikov1 , Andrey Ovchinnikov2 , Michael Agranat2 , Maria Semenova1 . 1 Lomonosov MSU, Moscow, Russia, 2 Joined Institute for High Temperatures RAS, Moscow, Russia Introduction: Embryo biopsy is a very important part of preimplantation genetic diagnosis. Nowadays the most popular method for embryo biopsy is mechanical method, but it requires timeconsuming pipette preparation, extensive practice and can lead to risk of embryo contamination and damage. We develop a new contactless technology for trophectoderm biopsy. The laser system with the functions of laser scalpel optical tweezers was created in the Joined Institute for High Temperatures RAS at the laboratory of laser plasma headed by M.B. Agranat. Method: In the experiment we used femtosecond laser impulses and nanosecond laser impulses. The laser beam was focused through 40× objective of invert microscope to a 2 mm diameter field. The experiment was created on the mouse embryos at the blastocyst stage at the beginning of hatching. At first we cut off some cells of the trophectoderm that were out of zona pellucida, with Cr:Forsterite seed oscillator and a regenerative amplifier, 100 fs, 10 Hz; then the system was switched to the regime of optical tweezers (cw fiber laser, 1064 nm) and the cut off cells were picked up to the optical trap and moved from the embryo. Results: The effectiveness of the procedure (a trophectoderm suitable for FISH analysis with alive embryo) was 62.2%. Conclusion: If we compare our method with the classical microsurgery technologies, laser-based biopsy offers several advantages. It allows less traumatic, more accurate and easier processing of the cells, while maintaining sterile requirements and decreasing the risk of embryo contamination.
PP-43 EFFICACY OF VITRIFICATION IN SIBLING OOCYTES Vijay Mangoli, Ranjana Mangoli, Sadhana Desai. Fertility Clinic & IVF Centre, Mumbai, India Background and Aim: Oocyte vitrification is now a well established procedure. However, its outcome varies between patients and also within subsequent cycles of same patient. This study was undertaken to assess efficacy of oocyte vitrification in terms of survival, fertilization, cleavage and blastocyst formation using sibling oocytes. Method: In couples undergoing IVF with normal semen parameters, when more than 16 MII oocytes were obtained, minimum 3 mature oocytes were vitrified using Quinn’s vitrification solutions and warmed on next day to inseminate with ICSI using same lot of sperm. Of the remaining oocytes, equal number was inseminated on day of Ovum Pick Up by ICSI as control. Oocytes from both control and study group were cultured for up to day 5 or 6. Their fertilization, cleavage, and blastocyst formation rates were compared.
S21 Results: Total 364 oocytes (182 in each group) from 52 patients were used for the present study. In the control group fertilization, cleavage and blastocyst formation rates were 141 (77.47%), 131 (92.9%), and 72 (54.9%) respectively. In the study group, survival rate was 171 (93.95%) and fertilization, cleavage, and blastocyst formation rates were 107 (62.5%), 96 (89.71%), and 49 (51.04%) respectively. Conclusion: Studying sibling oocytes is the most suitable option to assess efficacy of vitrification as, there are minimum variables in all related clinical and laboratory parameters. Though fertilization rate in study group was marginally less than control group, cleavage and blastocyst rates were comparable enough to confirm efficacy of oocyte vitrification. PP-44 REVITRIFIED BLASTOCYST TRANSFER: CLINICAL RESULTS Arancha Gal´ an Rivas, Sonia P´ erez-Albal´ a, Ana Cobo Cabal, Noelia Grau, Bel´ en Aparicio Ruiz, Marcos Meseguer Escriv´ a, María Jos´ e De Los Santos. IVI Valencia, Spain Background and Aim: High embryo survival after vitrification has increased the number of delayed transfers, when fresh transfer is not recommended. This increases the number of embryos undergoing several vitrification procedures before transfer. Our aim was to analyze survival and implantation rates of revitrified blastocysts that developed from embryos previously cryopreserved and thawed on cleavage stage (day +2 or day +3). Method: Retrospective study of 122 surplus revitrified blastocysts, previously cryopreserved on day +2 or day +3 of embryo development. Two groups were established according to the procedure undergone before the second vitrification: (1) extended culture from day +2 or day +3 to blastocyst (n = 82); (2) day +2 or day +3 with preimplantational genetic screening, PGS, (n = 40). Results: Overall survival rate of revitrified blastocyst was 81.1%, with statistically significant differences between both analyzed groups: 86.6% (1) vs 70.0% (2). Nevertheless we didn’t find differences in implantation rate (40.9% vs 32.1%), pregnancy rate (52.7% vs 50.0%) and abortion rate (27.5% vs 10%) between groups. Conclusion: Although revitrified bastocysts are not frequent and should be avoided when possible, these data show that similar pregnancy rates can be achieved. However, lower survival rate of the PGS-blastocysts was found, suggesting that the criteria to cryopreserve embryos is related to chromosomal normalcy rather than to embryo quality.
PP-43 EFFICACY OF VITRIFICATION IN SIBLING OOCYTES Vijay Mangoli, Ranjana Mangoli, Sadhana Desai. Fertility Clinic & IVF Centre, Mumbai, India Background and Aim: Oocyte vitrification is now a well established procedure. However, its outcome varies between patients and also within subsequent cycles of same patient. This study was undertaken to assess efficacy of oocyte vitrification in terms of survival, fertilization, cleavage and blastocyst formation using sibling oocytes. Method: In couples undergoing IVF with normal semen parameters, when more than 16 MII oocytes were obtained, minimum 3 mature oocytes were vitrified using Quinn’s vitrification solutions and warmed on next day to inseminate with ICSI using same lot of sperm. Of the remaining oocytes, equal number was inseminated on day of Ovum Pick Up by ICSI as control. Oocytes from both control and study group were cultured for up to day 5 or 6. Their fertilization, cleavage, and blastocyst formation rates were compared.
S21 Results: Total 364 oocytes (182 in each group) from 52 patients were used for the present study. In the control group fertilization, cleavage and blastocyst formation rates were 141 (77.47%), 131 (92.9%), and 72 (54.9%) respectively. In the study group, survival rate was 171 (93.95%) and fertilization, cleavage, and blastocyst formation rates were 107 (62.5%), 96 (89.71%), and 49 (51.04%) respectively. Conclusion: Studying sibling oocytes is the most suitable option to assess efficacy of vitrification as, there are minimum variables in all related clinical and laboratory parameters. Though fertilization rate in study group was marginally less than control group, cleavage and blastocyst rates were comparable enough to confirm efficacy of oocyte vitrification. PP-44 REVITRIFIED BLASTOCYST TRANSFER: CLINICAL RESULTS Arancha Gal´ an Rivas, Sonia P´ erez-Albal´ a, Ana Cobo Cabal, Noelia Grau, Bel´ en Aparicio Ruiz, Marcos Meseguer Escriv´ a, María Jos´ e De Los Santos. IVI Valencia, Spain Background and Aim: High embryo survival after vitrification has increased the number of delayed transfers, when fresh transfer is not recommended. This increases the number of embryos undergoing several vitrification procedures before transfer. Our aim was to analyze survival and implantation rates of revitrified blastocysts that developed from embryos previously cryopreserved and thawed on cleavage stage (day +2 or day +3). Method: Retrospective study of 122 surplus revitrified blastocysts, previously cryopreserved on day +2 or day +3 of embryo development. Two groups were established according to the procedure undergone before the second vitrification: (1) extended culture from day +2 or day +3 to blastocyst (n = 82); (2) day +2 or day +3 with preimplantational genetic screening, PGS, (n = 40). Results: Overall survival rate of revitrified blastocyst was 81.1%, with statistically significant differences between both analyzed groups: 86.6% (1) vs 70.0% (2). Nevertheless we didn’t find differences in implantation rate (40.9% vs 32.1%), pregnancy rate (52.7% vs 50.0%) and abortion rate (27.5% vs 10%) between groups. Conclusion: Although revitrified bastocysts are not frequent and should be avoided when possible, these data show that similar pregnancy rates can be achieved. However, lower survival rate of the PGS-blastocysts was found, suggesting that the criteria to cryopreserve embryos is related to chromosomal normalcy rather than to embryo quality.