PP114-MON CHANGE IN FOOD INTAKE PATTERNS LEADING TO TYPE 2 DIABETES IN INDIANS

Nutrition and chronic diseases II Methods: C57black6 mice were divided into the control group, which received standard CE-2 diet (n = 8), and the tryptophan group, which received CE-2 diet containing 0.5% l-tryptophan (n = 8). All mice were given a solution of 3.5% DSS daily for 12 days to induce colitis. Changes of body weight and bloody stool frequency were monitored. At 12 days after initial DSS administration, all mice were sacrificed. Middle colon tissues were examined histologically, and nitrotyrosine levels in the colon tissues of the DSS-induced control and tryptophan groups were compared. Further, nitrite and nitrate (NOx) levels in urine were compared among untreated animals, DSStreated control and tryptophan groups. Results: Body weight reduction and blood stool frequencies were significantly ameliorated in the tryptophan group throughout 2 12 days after the initial DSS administration, compared with the control group. Moreover, tryptophan ameliorated histological changes characteristic of colitis. Nitrotyrosine level (ng/g protein) in the colonic tissues was significantly reduced to 61.1±11.0 in the tryptophan group, compared with 87.8±7.4 in the control group. NOx levels in urine (mM) in the (DSS-induced) control group (821±181) and the tryptophan group (780±162) were significantly increased compared with the mice without DSS administration group (496±87). However, there were no siginificantly differences between the (DSS-induced) control group and the tryptophan group. Conclusion: Tryptophan treatment ameliorated DSSinduced colitis in this study. One of the anti-colitis mechanisms of tryptophan appears to be an anti-oxidative stress effect, and may be not via suppression of nitric oxide (NO) generation. Disclosure of Interest: None Declared

PP114-MON CHANGE IN FOOD INTAKE PATTERNS LEADING TO TYPE 2 DIABETES IN INDIANS V.L. Pratti1 , C.S. Chirumamilla2 . 1 Faculty of Bioscence engineering, University of Gent, gent, Belgium; 2 Norwegian Technological University, Trondheim, Norway Rationale: A cross sectional study was conducted to identify alterations in lifestyle of type 2 diabetes patients in India and to compare the consumption patterns with patients in Belgium. Methods: The study was conducted on 60 subjects (n = 30 from India and n = 30 from Belgium). Random sample of newly detected type 2 diabetes patients (2 years) reported to hospital in Hyderabad city, India, patients reported to Dietician at Ghent University Hospital, Gent, Belgium are selected for study. Food Frequency Questionnaire (FFQ) was used in India to estimate intake, patient’s diet recall was used in Belgium. Becel Institute nutrition software was used to estimate food intake of Belgians. Statistical package for Social Sciences (SPSS) version 18.0 was used for analysis. Independent sample t-test was used to compare consumption and nutrient intake of subjects. Chi-square analysis was used to compare frequencies. Results: See the table.

157

Age (y) HbA1c (%) Weight (kg) Height (cm) BMI (kg/m2 ) Energy (kcal) Protein (g) Fat (g) Carbohydrates (g) Fiber (g)

India

Belgium

p-value

47 8.95 70 168 23.8 2144±327 72±12 50±11 349±57 11±2

57

p < 0.0001

94 167 33.4 2630±580 108±27 101±31 307±87 38±12

p < 0.0001 p = 0.845 p < 0.0001 p < 0.0001 p < 0.0001 p < 0.0001 p = 0.03 p < 0.0001

Conclusion: Urbanization has drastically changed lifestyle and shifted dietary habits of the population in India. Traditional food preparations with staple cereals and grains were substituted with polished rice, refined wheat and grain products which lead to substantial reduction in fiber content of diet. High fat, high sugar intake, low physical activity, sedentary lifestyle incline to obesity, cardiovascular and other chronic diseases thus leading to undiagnosed insulin resistance when neglected consequently causing type 2 diabetes. Disclosure of Interest: V. Pratti Grant/Research Support from: VLIR-UOS, C. Chirumamilla: None Declared

PP115-MON Outstanding abstract ASSOCIATION BETWEEN VITAMIN D, INTACT PTH AND BLOOD PRESSURE: CUT POINT OF PTH FOR HYPERTENSION V.C. Garcia1 , N.J. Schuch1 , M.O. Cicca1 , S.R.G. Ferreira1 , L.A. Martini1 . 1 School of Public Health, University of S˜ ao Paulo, S˜ ao Paulo, Brazil Rationale: Low vitamin D has been associated with hypertension. Thus, the aim of this study was to evaluated whether vitamin D status biomarkers are related to blood pressure (BP) in adults. Methods: We evaluated 332 individuals (62% women), living in S˜ ao Paulo, Brazil, during all seasons. Anthropometric measurements, BP and a fasting blood sample were obtained. Serum concentration of 25(OH)D3 was measured by HPLC and iPTH by electrochemiluminescence. BP was obtained by mean of two measures. Vitamin D insufficiency was defined by 25(OH)D3 75 nmol/L, elevated iPTH >65 pg/mL and high BP 140/90 mmHg. Pearson’scorrelation and ROC Curve was performed by SPSS (v.18.0); significance level was p < 0.05. Results: Mean age was 50(15) years, BMI 29(6)kg/m2 , waist circumference 97(13) cm, serum calcium 9.3 (0.4) mg/dL and iPTH 41 (18) pg/mL. Elevated iPTH was present in 12% of the sample. Mean BP was 129/80 (18/11) mmHg. Mean vitamin D was 56 (17) nmol/L. Vitamin D insufficiency was present in 86% of the sample. No significant association was observed between BP and vitamin D insufficiency. However, a positive correlation was observed between iPTH with systolic (r = 0.168; p = 0.002) and diastolic BP (r = 0.168; p = 0.002), waist circumference (r = 0.172; p = 0.002) and BMI (r = 0.125; p = 0.023). ROC curve analysis comparing iPTH in prediction of hypertension showed AUC of 0.602 [IC95% AUC: (0.541; 0.663); p = 0.001] and cut point suggested