- Email: [email protected]
PP143-SUN: Effect of Dietary Amino Acids on Liver Function of Fructose Fed Rats
Nutrition and chronic diseases Conclusion: In our study we found that among diabetics with poor glycemic control proportion of undiagnosed ADHD almost 8 times higher as compared to patients with good glycemic control. Disclosure of Interest: E. Merzon Grant/Research Support from: Abbot, Novartis, E. Kitai: None Declared, A. Golan-Cohen: None Declared.
PP142-SUN HOME ENTERAL NUTRITION (HEN) HELPS TO REDUCE COMPLICATIONS, LENGTH OF STAY AND HEALTH-CARE COSTS IN ADULTS S. Klek1 , A. Hermanowicz2 , G. Dziwiszek3 , K. Matysiak4 , K. Szczepanek1 , P. Szybinski1 , T. Kowalczyk1 , K. Figula1 , R. Choruz1 , A. Galas5 . 1 General and Oncology Surgery Unit, Stanley Dudrick’s Memorial Hospital, Skawina, 2 Department of Pediatric Surgery, Medical University of Bialystok, Bialystok, 3 Home Enteral Nutrition Unit, Stomed, Ostroleka, 4 Chair of General, Gastroenterology and Oncology and Plastic Surgery, Medical University of Poznan, Poznan, 5 Chair of Epidemiology and Preventive Medicine, Jagiellonian University Medical College, Krakow, Poland Rationale: Home enteral nutrition (HEN) has always been recognized as a life-saving procedure, but with the ongoing economic crisis influencing health care, its cost-effectiveness has been questioned recently. The unique reimbursement situation in Poland enabled the otherwise ethically unacceptable, hence unavailable, comparison of the period of no-feeding and the long-term feeding and the subsequent analyses of the clinical value of the latter and its cost-effectiveness. Methods: The observational multicenter study in the group of 314 adult HEN patients (314 adults: 151 F and 163 M, mean age 59.3 SD = 19.8) was performed between January 2007 and July 2013. Two twelve-month periods were compared. During the first one patients were tube fed with home-made diet without monitoring, while during the other the complex HEN was carried out. The latter included tube feeding and complex monitoring from nutrition support team. Number of complications, hospital admissions, length of hospital stay (LOS), biochemical and anthropometric parameters and costs of hospitalization were compared. Results: Implementation of HEN enabled weight gain and stabilized liver function in both age groups, but it hardly influenced other tests. HEN implementation reduced the incidence of infectious complications (32.8 vs 13.4%, p < 0.001), the number of hospital admissions (1.84 before and 1.11 after HEN, P < 0.001) and LOS (36.7 vs 9.6 days, p < 0.001). Mean annual costs of hospitalization were reduced from 5513.12 to 1619.37 USD. Conclusion: The study proved that HEN improves the clinical outcome and decreases health-care costs. Disclosure of Interest: S. Klek Speaker bureau of: B Braun Nutricia Baxter Fresenius Nestle, A. Hermanowicz: None Declared, G. Dziwiszek: None Declared, K. Matysiak: None Declared, K. Szczepanek: None Declared, P. Szybinski: None Declared, T. Kowalczyk: None Declared, K. Figula: None Declared, R. Choruz: None Declared, A. Galas: None Declared.
S73 PP143-SUN EFFECT OF DIETARY AMINO ACIDS ON LIVER FUNCTION OF FRUCTOSE FED RATS P. Jegatheesan1 , S.D. Beutheu1 , G. Ventura1 , P. Marquet-de-Roug´ e1 , G. Sarfati2 , E. Nubret1 , L. Cynober1,2 , 1,2 1 J.-P. De Bandt . Laboratoire de Biologie de la Nutrition, Universit´ e Paris Descartes, 2 Clinical chemistry department, Hˆ opitaux Universitaires Paris Centre, APHP, Paris, France Rationale: Fructose consumption may be one of the key factors in the development of non-alcoholic fatty liver disease (NAFLD). Nevertheless adverse effects of fructose seem to be limited when it is consumed in a moderate and/or in a balanced diet. Our aim was to determine the effect of fructose diet along with non-essential amino acids (NEAA) in the development of NAFLD induced by high fructose. Methods: 18 males Sprague-Dawley rats (225 250 g) were randomly allocated to 3 groups and received a 60% fructose diet for 8 weeks either alone (F) or supplemented with NEAA (aspartate, glycine, histidine, proline, alanine, serine) (FNEAA). One group received a standard diet (40% cornstarch, 20% maltodextrin) with NEAA (CNEAA). Parameters studied were: body weight and food intake daily, hepatic steatosis (liver/body weight and triglyceride content), and metabolic status (plasma glucose, insulin, and triglycerides) at the end of the feeding period. Statistical analysis was performed with a Kruskal Wallis test followed by a Dunn test. Results: Food intake and body weight gain were similar between the 3 groups. Fructose led to a significant increase in liver/body weight, liver triglycerides and plasma triglycerides, which was attenuated by NEAA supply. Plasma glucose tended to increase in fructose fed rat while there was no observable alteration in insulinemia. F Plasma triglycerides (mM) Liver/body weight ratio (%) Liver triglycerides (mmol)
FNEAA a
0.88±0.24 3.6±0.2 a 721.5±98.5 a
CNEAA b
0.4±0.05 2.7±0.03 b 150±35.4 b
0.39±0.09 b 2.4±0.04 b 386.0±97.7 c
Data are expressed as mean±SEM, in a row a=b / =c: / p < 0.05.
Conclusion: NEAA intake or nitrogen supply protect against fructose-induced NAFLD. Our present mechanistic analysis suggests that the NEAA acts via the regulation of genes involved in hepatic triglyceride VLDL formation (Microsomal triglyceride transfer protein) but also in hepatic inflammation (Toll-like receptor 4). Disclosure of Interest: None Declared.
PP144-SUN DETERMINANTS OF VITAMIN D DEFICIENCY IN THE BALTIMORE LONGITUDINAL STUDY OF AGING M. Moberg1 , P. Elango2 , L. Ferrucci3 , M.A. Spruit4 , E.F. Wouters4 , E. Rutten4 . 1 Section of Respiratory Medicine, Hvidovre University Hospital, Hvidovre, Denmark; 2 Longitudinal Studies Section, Translational Gerontology Branch, 3 Longitudinal Studies Section, Clinical Research Branch, National Institute on Aging, Baltimore, Maryland, United States; 4 Research and education, CIRO+, Centre of expertise for chronic organ failure, Horn, Netherlands Rationale: Vitamin D deficiency (VDD) is common in patients with chronic obstructive pulmonary disease (COPD) and has
PP142-SUN HOME ENTERAL NUTRITION (HEN) HELPS TO REDUCE COMPLICATIONS, LENGTH OF STAY AND HEALTH-CARE COSTS IN ADULTS S. Klek1 , A. Hermanowicz2 , G. Dziwiszek3 , K. Matysiak4 , K. Szczepanek1 , P. Szybinski1 , T. Kowalczyk1 , K. Figula1 , R. Choruz1 , A. Galas5 . 1 General and Oncology Surgery Unit, Stanley Dudrick’s Memorial Hospital, Skawina, 2 Department of Pediatric Surgery, Medical University of Bialystok, Bialystok, 3 Home Enteral Nutrition Unit, Stomed, Ostroleka, 4 Chair of General, Gastroenterology and Oncology and Plastic Surgery, Medical University of Poznan, Poznan, 5 Chair of Epidemiology and Preventive Medicine, Jagiellonian University Medical College, Krakow, Poland Rationale: Home enteral nutrition (HEN) has always been recognized as a life-saving procedure, but with the ongoing economic crisis influencing health care, its cost-effectiveness has been questioned recently. The unique reimbursement situation in Poland enabled the otherwise ethically unacceptable, hence unavailable, comparison of the period of no-feeding and the long-term feeding and the subsequent analyses of the clinical value of the latter and its cost-effectiveness. Methods: The observational multicenter study in the group of 314 adult HEN patients (314 adults: 151 F and 163 M, mean age 59.3 SD = 19.8) was performed between January 2007 and July 2013. Two twelve-month periods were compared. During the first one patients were tube fed with home-made diet without monitoring, while during the other the complex HEN was carried out. The latter included tube feeding and complex monitoring from nutrition support team. Number of complications, hospital admissions, length of hospital stay (LOS), biochemical and anthropometric parameters and costs of hospitalization were compared. Results: Implementation of HEN enabled weight gain and stabilized liver function in both age groups, but it hardly influenced other tests. HEN implementation reduced the incidence of infectious complications (32.8 vs 13.4%, p < 0.001), the number of hospital admissions (1.84 before and 1.11 after HEN, P < 0.001) and LOS (36.7 vs 9.6 days, p < 0.001). Mean annual costs of hospitalization were reduced from 5513.12 to 1619.37 USD. Conclusion: The study proved that HEN improves the clinical outcome and decreases health-care costs. Disclosure of Interest: S. Klek Speaker bureau of: B Braun Nutricia Baxter Fresenius Nestle, A. Hermanowicz: None Declared, G. Dziwiszek: None Declared, K. Matysiak: None Declared, K. Szczepanek: None Declared, P. Szybinski: None Declared, T. Kowalczyk: None Declared, K. Figula: None Declared, R. Choruz: None Declared, A. Galas: None Declared.
S73 PP143-SUN EFFECT OF DIETARY AMINO ACIDS ON LIVER FUNCTION OF FRUCTOSE FED RATS P. Jegatheesan1 , S.D. Beutheu1 , G. Ventura1 , P. Marquet-de-Roug´ e1 , G. Sarfati2 , E. Nubret1 , L. Cynober1,2 , 1,2 1 J.-P. De Bandt . Laboratoire de Biologie de la Nutrition, Universit´ e Paris Descartes, 2 Clinical chemistry department, Hˆ opitaux Universitaires Paris Centre, APHP, Paris, France Rationale: Fructose consumption may be one of the key factors in the development of non-alcoholic fatty liver disease (NAFLD). Nevertheless adverse effects of fructose seem to be limited when it is consumed in a moderate and/or in a balanced diet. Our aim was to determine the effect of fructose diet along with non-essential amino acids (NEAA) in the development of NAFLD induced by high fructose. Methods: 18 males Sprague-Dawley rats (225 250 g) were randomly allocated to 3 groups and received a 60% fructose diet for 8 weeks either alone (F) or supplemented with NEAA (aspartate, glycine, histidine, proline, alanine, serine) (FNEAA). One group received a standard diet (40% cornstarch, 20% maltodextrin) with NEAA (CNEAA). Parameters studied were: body weight and food intake daily, hepatic steatosis (liver/body weight and triglyceride content), and metabolic status (plasma glucose, insulin, and triglycerides) at the end of the feeding period. Statistical analysis was performed with a Kruskal Wallis test followed by a Dunn test. Results: Food intake and body weight gain were similar between the 3 groups. Fructose led to a significant increase in liver/body weight, liver triglycerides and plasma triglycerides, which was attenuated by NEAA supply. Plasma glucose tended to increase in fructose fed rat while there was no observable alteration in insulinemia. F Plasma triglycerides (mM) Liver/body weight ratio (%) Liver triglycerides (mmol)
FNEAA a
0.88±0.24 3.6±0.2 a 721.5±98.5 a
CNEAA b
0.4±0.05 2.7±0.03 b 150±35.4 b
0.39±0.09 b 2.4±0.04 b 386.0±97.7 c
Data are expressed as mean±SEM, in a row a=b / =c: / p < 0.05.
Conclusion: NEAA intake or nitrogen supply protect against fructose-induced NAFLD. Our present mechanistic analysis suggests that the NEAA acts via the regulation of genes involved in hepatic triglyceride VLDL formation (Microsomal triglyceride transfer protein) but also in hepatic inflammation (Toll-like receptor 4). Disclosure of Interest: None Declared.
PP144-SUN DETERMINANTS OF VITAMIN D DEFICIENCY IN THE BALTIMORE LONGITUDINAL STUDY OF AGING M. Moberg1 , P. Elango2 , L. Ferrucci3 , M.A. Spruit4 , E.F. Wouters4 , E. Rutten4 . 1 Section of Respiratory Medicine, Hvidovre University Hospital, Hvidovre, Denmark; 2 Longitudinal Studies Section, Translational Gerontology Branch, 3 Longitudinal Studies Section, Clinical Research Branch, National Institute on Aging, Baltimore, Maryland, United States; 4 Research and education, CIRO+, Centre of expertise for chronic organ failure, Horn, Netherlands Rationale: Vitamin D deficiency (VDD) is common in patients with chronic obstructive pulmonary disease (COPD) and has