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PP213 DETERMINATION OF PLASMATIC LEVELS OF VITAMIN A, E, C, B12 AND FOLIC ACID IN EACH STAGE OF COLORECTAL CANCER
106 iodine, there were no significant differences in subclass of iodine between the day before and 4 weeks after initiation of TPN, except for T3 (0.7±0.3 vs 0.8±0.2, P < 0.01). Conclusion: Patients who receive long-term TPN administration might have accumulation of zinc and iron. Disclosure of Interest: None declared
PP213 DETERMINATION OF PLASMATIC LEVELS OF VITAMIN A, E, C, B12 AND FOLIC ACID IN EACH STAGE OF COLORECTAL CANCER M. Hronek1 , D. Solichova2 , R. Hyspler2 , A. Ticha2 , Z. Zadak2 . 1 Dept Biol and Med Sci, Charles University in Prague, Faculty of Pharmacy, 2 Dept Research and Development, Teaching Hospital, Hradec Kralove, Czech Republic Rationale: The aim of this study was to determine plasmatic levels of vitamins A, E, C B12 and folic acid that may be involved in the antioxidative activity process in cancerogenesis in patients with colorectal cancer (CCa) utilizable for nutritional support. Methods: Plasmatic levels of vitamins with antioxidative activity in two cohorts were determined. In the first group, nineteen men and eleven women in age 64±12 y with confirmed colorectal cancer, before treatment, were observed. 4 patients were in stage Dukes A, 16 patients in Dukes B, 5 patients in Dukes C and 4 patients in Dukes D. In the control cohort nineteen men and eleven women with benign disorders, such as inguinal hernia or varices without inflammatory responses were examined. Results: In all stages Dukes of CCa plasmatic levels of vitamin A were significantly lower than in control subjects (P =0.016). In stage Dukes A no statistical difference between the two cohorts was found. Significant differences in plasmatic levels by unpaired t-test were observed between lower plasmatic level in Dukes B of vitamin A (1.84±0.18 mmol/l) versus the control group (2.26±0.12 mmol/l), vitamin E (20.53±2.03 mmol/l versus 25.83±1.09 mmol/l); in Dukes C of vitamin C (25.83±1.09 mmol/l versus 36.96±5.27 mmol/l); in Dukes D of vitamin A (1.48±0.43 mmol/l versus (2.26±0.12 mmol/l). Conclusion: In the pilot study we have proved there are lower plasmatic levels of some vitamines with antioxidative activity in each stage of CCa. In regards of this deficit, to think about vitamins perorals or parenteral supplementation as part and parcel of nutritive support is important. Disclosure of Interest: None declared
PP214 IMMUNE IMPAIRMENT INDUCED BY PSYCHOLOGICAL STRESS WAS PREVENTED BY DIETARY SUPPLEMENTATION WITH APPLE POLYPHENOL ENRICHED EXTRACT IN A WATER AVOIDANCE STRESS (WAS) MOUSE MODEL E. Vazquez1 , M.J. Martín1 , A. Barranco1 , M. Ramirez1 , R. Rueda1 . 1 Discovery Technology R&D, Abbott Laboratories, Granada, Spain Rationale: The hypothalamic-pituitary-adrenocortical (HPA) system is a critical mediator of stress responses in
Poster presentations humans and rodents. This HPA axis is a potent modulator of immune system.The aim of this work was to test the potential immunoregulatory capabilities of an apple extract enriched in polyphenols (AP) in a mouse model of psychological stress Methods: 36 female Balb/C 8 weeks old mice were randomized in three groups: sham no stress (SG), control stress (CG) (both received AIN-93M control diet) and AP stress group (APG), fed on the same diet containing 0.3% AP (n = 12). We have applied a water-avoidance stress (WAS) protocol, consisting of placing a mouse for 1 h on a small platform located in the middle of a plastic container filled with water up to 1 cm below the platform during 10 consecutive days. Sham mice were kept in containers without water. Blood was collected to measure serum corticosterone immediately after the last stress session and subsequently mice were sacrificed. Different morphohistolologic parameters were recorded. Spleen and thymus lymphocytes were isolated and cultured to assess cell proliferation. IL-2 and IL-10 cytokine production as well as cell surface markers were measured by flow cytometry. Results: Chronic psychological stress induced an increase in serum corticosterone (p < 0.05), and a decrease in the number of spleen cells (p < 0.05) as well as in spleen proliferative rate (p < 0.05). The cytokine pattern in culture supernatants was also affected by stress (p < 0.05). Dietary AP were able to prevent all of these pernicious stress-driven effects (p < 0.05), since the data showed APG and CG were not statistically different for the above mentioned parameters. Conclusion: AP is an effective ingredient for preventing the effects of stress on some biochemical and immunological parameters. Disclosure of Interest: None declared
PP215 THE RELATIONSHIP BETWEEN BLOOD AND LIVER CONCENTRATIONS OF SELENIUM AND CHANGES DURING THE ACUTE PHASE RESPONSE J. Logue1 , A. Duncan2 , C. Roxburgh3 , P. Horgan3 , D.S. O’Reilly2 . 1 Cardiovascular and Medical Sciences, University of Glasgow, 2 Clinical Biochemistry, Glasgow Royal Infirmary, 3 Department of Surgery, University of Glasgow, Glasgow, United Kingdom Rationale: Plasma selenium concentration falls during the acute phase response making it a potentially unreliable measure of selenium status. We have examined the relationship between liver selenium and red blood cell (RBC) and plasma selenium concentration, and RBC glutathione peroxidase (GPX) activity, before and during the acute phase response. Methods: 31 patients (14 male, 17 female; mean age 64) who were due to have a liver resection for metastatic colorectal carcinoma were recruited. Blood were taken immediately pre-operatively (day 0) and 24, 48 and 72 hours post-operatively (day 1 3). Samples of healthy liver were taken from the resected lobe. Plasma, RBC and tissue selenium concentrations were measured using ICPMS after acid digestion. RBC GPX activity was measured by spectrophotometry. Simple Linear regression was used
PP213 DETERMINATION OF PLASMATIC LEVELS OF VITAMIN A, E, C, B12 AND FOLIC ACID IN EACH STAGE OF COLORECTAL CANCER M. Hronek1 , D. Solichova2 , R. Hyspler2 , A. Ticha2 , Z. Zadak2 . 1 Dept Biol and Med Sci, Charles University in Prague, Faculty of Pharmacy, 2 Dept Research and Development, Teaching Hospital, Hradec Kralove, Czech Republic Rationale: The aim of this study was to determine plasmatic levels of vitamins A, E, C B12 and folic acid that may be involved in the antioxidative activity process in cancerogenesis in patients with colorectal cancer (CCa) utilizable for nutritional support. Methods: Plasmatic levels of vitamins with antioxidative activity in two cohorts were determined. In the first group, nineteen men and eleven women in age 64±12 y with confirmed colorectal cancer, before treatment, were observed. 4 patients were in stage Dukes A, 16 patients in Dukes B, 5 patients in Dukes C and 4 patients in Dukes D. In the control cohort nineteen men and eleven women with benign disorders, such as inguinal hernia or varices without inflammatory responses were examined. Results: In all stages Dukes of CCa plasmatic levels of vitamin A were significantly lower than in control subjects (P =0.016). In stage Dukes A no statistical difference between the two cohorts was found. Significant differences in plasmatic levels by unpaired t-test were observed between lower plasmatic level in Dukes B of vitamin A (1.84±0.18 mmol/l) versus the control group (2.26±0.12 mmol/l), vitamin E (20.53±2.03 mmol/l versus 25.83±1.09 mmol/l); in Dukes C of vitamin C (25.83±1.09 mmol/l versus 36.96±5.27 mmol/l); in Dukes D of vitamin A (1.48±0.43 mmol/l versus (2.26±0.12 mmol/l). Conclusion: In the pilot study we have proved there are lower plasmatic levels of some vitamines with antioxidative activity in each stage of CCa. In regards of this deficit, to think about vitamins perorals or parenteral supplementation as part and parcel of nutritive support is important. Disclosure of Interest: None declared
PP214 IMMUNE IMPAIRMENT INDUCED BY PSYCHOLOGICAL STRESS WAS PREVENTED BY DIETARY SUPPLEMENTATION WITH APPLE POLYPHENOL ENRICHED EXTRACT IN A WATER AVOIDANCE STRESS (WAS) MOUSE MODEL E. Vazquez1 , M.J. Martín1 , A. Barranco1 , M. Ramirez1 , R. Rueda1 . 1 Discovery Technology R&D, Abbott Laboratories, Granada, Spain Rationale: The hypothalamic-pituitary-adrenocortical (HPA) system is a critical mediator of stress responses in
Poster presentations humans and rodents. This HPA axis is a potent modulator of immune system.The aim of this work was to test the potential immunoregulatory capabilities of an apple extract enriched in polyphenols (AP) in a mouse model of psychological stress Methods: 36 female Balb/C 8 weeks old mice were randomized in three groups: sham no stress (SG), control stress (CG) (both received AIN-93M control diet) and AP stress group (APG), fed on the same diet containing 0.3% AP (n = 12). We have applied a water-avoidance stress (WAS) protocol, consisting of placing a mouse for 1 h on a small platform located in the middle of a plastic container filled with water up to 1 cm below the platform during 10 consecutive days. Sham mice were kept in containers without water. Blood was collected to measure serum corticosterone immediately after the last stress session and subsequently mice were sacrificed. Different morphohistolologic parameters were recorded. Spleen and thymus lymphocytes were isolated and cultured to assess cell proliferation. IL-2 and IL-10 cytokine production as well as cell surface markers were measured by flow cytometry. Results: Chronic psychological stress induced an increase in serum corticosterone (p < 0.05), and a decrease in the number of spleen cells (p < 0.05) as well as in spleen proliferative rate (p < 0.05). The cytokine pattern in culture supernatants was also affected by stress (p < 0.05). Dietary AP were able to prevent all of these pernicious stress-driven effects (p < 0.05), since the data showed APG and CG were not statistically different for the above mentioned parameters. Conclusion: AP is an effective ingredient for preventing the effects of stress on some biochemical and immunological parameters. Disclosure of Interest: None declared
PP215 THE RELATIONSHIP BETWEEN BLOOD AND LIVER CONCENTRATIONS OF SELENIUM AND CHANGES DURING THE ACUTE PHASE RESPONSE J. Logue1 , A. Duncan2 , C. Roxburgh3 , P. Horgan3 , D.S. O’Reilly2 . 1 Cardiovascular and Medical Sciences, University of Glasgow, 2 Clinical Biochemistry, Glasgow Royal Infirmary, 3 Department of Surgery, University of Glasgow, Glasgow, United Kingdom Rationale: Plasma selenium concentration falls during the acute phase response making it a potentially unreliable measure of selenium status. We have examined the relationship between liver selenium and red blood cell (RBC) and plasma selenium concentration, and RBC glutathione peroxidase (GPX) activity, before and during the acute phase response. Methods: 31 patients (14 male, 17 female; mean age 64) who were due to have a liver resection for metastatic colorectal carcinoma were recruited. Blood were taken immediately pre-operatively (day 0) and 24, 48 and 72 hours post-operatively (day 1 3). Samples of healthy liver were taken from the resected lobe. Plasma, RBC and tissue selenium concentrations were measured using ICPMS after acid digestion. RBC GPX activity was measured by spectrophotometry. Simple Linear regression was used