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PP280-MON: Outstanding abstract:Potential Intake of Vitamin A and D Through Branded Intravenous Lipid Emulsions
Vitamins, antioxidants and minerals PP279-MON Outstanding abstract MICRONUTRIENT LOSS DURING RENAL REPLACEMENT THERAPY FOR ACUTE KIDNEY INIJURY W. Oh1,2 , M. Rigby1 , B. Mafrici1 , A. Sharman2 , D. Harvey2 , S. Welham3 , R. Mahajan2 , D. Gardner4 , M. Devonald1,2 . 1 Renal and Transplant Unit, Nottingham University Hospitals NHS Trust, 2 School of Clinical Sciences, 3 School of Biosciences, 4 School of Veterinary Medicine and Science, University of Nottingham, Nottingham, United Kingdom Rationale: Patients with acute kidney injury (AKI) may require renal replacement therapy (RRT), which could result in losses of water-soluble micronutrients. The aim of this study is to quantify these losses during RRT in patients with AKI and to compare losses in intermittent haemodialysis (IHD) and sustained low-efficiency diafiltration (SLED-F). Methods: Adults with AKI requiring RRT are recruited into a single centre prospective observational study. Blood and RRT effluent are sampled at baseline, mid- and end-session from each participant during their first two RRT treatments. Samples are analysed using high performance liquid chromatography and inductively coupled mass spectrometry. Total micronutrient losses during dialysis are calculated by multiplying known concentrations by total volume of RRT effluent, adjusted to plasma concentrations and dialysis dose. Data are analysed by restricted maximum likelihood estimating equations. Full data are available for 7 IHD and 4 SLED-F patients. Results: Total plasma concentrations of all standard amino acids at baseline were similar between patients receiving IHD vs. SLED-F (992±120 vs. 1036±150 mmol/L, respectively) and did not change significantly by the end of each dialysis session (837±120 vs. 844±140 mmol/L, respectively) or after correction for Kt/V (IHD, 0.34 vs. SLED-F, 1.0). The unadjusted loss of amino acids was similar between dialysis types (IHD, 5.50±0.94 vs. SLED-F, 4.01±1.32 mmol/L). Trace elements were measurable in dialysate. The rate of loss of any trace element did not appear to differ throughout the course of a IHD or SLED-F session. There was no difference in trace element losses between RRT modalities. Conclusion: Unadjusted micronutrient losses in IHD and SLED-F are similar. It is unclear whether these losses are clinically significant. This will require further investigation, as is comparison with losses during continuous veno-venous haemofiltration. Disclosure of Interest: None Declared.
PP280-MON Outstanding abstract POTENTIAL INTAKE OF VITAMIN A AND D THROUGH BRANDED INTRAVENOUS LIPID EMULSIONS M.L. Forchielli1 , M. Conti2 , A. Stancari3 , S. Maselli3 , A.M. Guarguaglini3 , L. Loredana Bruno3 , D. Patrono2 , A. Pession1 , C. Puggioli3 , G. Bersani4 . 1 Pediatrics, university of Bologna Italy, 2 Analysis Laboratory, 3 Pharmacy Service, S Orsola-Malpighi Hosp, 4 Consulting Pharmacist, Bologna, Italy Rationale: No data exist for the vitamin A group and vitamin D2/D3 content in branded intravenous lipid emulsions (LEs). Our goal is to evaluate and quantify their concentrations in different LEs to assess whether they are clinically relevant. Methods: Analyses were carried out in triplicates on six LEs: 1) 30% soybean oil-based, 2) 20% olive-soybean oil based, 3) 10+10% soybean-medium-chain-triglyceride (MCT)
S233 coconut oil based, 4) 20% soybean-olive-MCT-fish-oil based, 5) 20% soybean-MCT-fish-oil based and 6) 10% pure fish oil based, respectively. Retinol and ergo-chole-calciferol were analyzed and quantified by a novel quali-quantitative Liquid Chromatography-Mass Spectrometry method after potassium hydroxide saponification, hexane extraction, reverse phase-liquid chromatography and specific multiple-residualmonitoring for retinol and vitamin D2/D3. The method proved to be extremely effective in term of sensitivity (3 ppb), specificity (mass spectrum), linearity (R2 = 0.99) and accuracy (CV <5%) for vitamin measurements in a complex lipid matrix. Statistics were provided by t-test. Results: Average retinol content was in the range of 0.2 1.0 mg/L in 1, 2, and 3 LEs, whereas it was higher (1 2 mg/L) in LEs containing fish-oil. Vitamin D content was in the range of 0.005 0.01 mg/L in fish-oil based LEs, but absent in those LEs containing purely vegetable oils. Conclusion: This study shows that retinol and vitamin D contents are variably present in LEs based on different lipid sources. These amounts need to be taken into account to provide correct intakes to patients. Disclosure of Interest: None Declared.
PP281-MON Outstanding abstract A RANDOMIZED CONTROLLED DOUBLE BLINDED CLINICAL TRIAL OF VITAMIN D3 SUPPLEMENTATION ON OBESITY PARAMETERS IN OBESE TYPE 2 DIABETES SUBJECTS IN THE UAE A. Sadiya1 . 1 Lifestyle Clinic, Rashid Centre for Diabetes and Research, Ajman, United Arab Emirates Rationale: The co-existence of vitamin D deficiency with obesity and type 2 diabetes is highly prevalent in this region. The objective of this study was to study the effect of Vitamin D3 supplementation on obesity parameters in obese Type 2 diabetes Emirati population in United Arab Emirates. Methods: A randomized double-blind clinical trial was conducted for 6 months with 87 vitamin D deficient obese, type 2 diabetic subjects. The study was divided into 3 phases; in Phase I the Vitamin D group (n = 45) received 6000IU Vitamin D3 /day (3 months), in Phase II 3000IU Vitamin D3/day or the placebo group (n = 42). During follow up both the arms were supplemented with 2200IU vitamin D3 /day for 6 months (Phase III). Results: At the baseline a significant (p < 0.01) positive association between body fat mass and body weight (r = 0.917) muscle mass (r = 0.47), water mass (r = 0.542), waist circumference (r = 0.815) and serum PTH (r = 0.284) was observed. On supplementation no significant changes were observed in body weight ( 0.8%, p = ns), BMI, waist circumference or fat mass ( 1.1%, p = ns). However, serum 25(OH)D peaked in phase I (28.5±9.2 vs 77.2±30.1, p = 0.003) followed by a decrease in phase II (61.4±18.8, p = 0.006), and consequently the PTH decreased in phase II (5.89±2.36 vs 5.89±2.36, p < 0.01) compared to baseline in vitamin D group. Conclusion: There is no significant influence of vitamin D supplementation on weight and fat loss in vitamin D deficient obese type 2 diabetic subjects of Arab ethnicity. However it normalized vitamin D status and reduced the incidence of eucalcemic PTH elevation. Disclosure of Interest: None Declared.
PP280-MON Outstanding abstract POTENTIAL INTAKE OF VITAMIN A AND D THROUGH BRANDED INTRAVENOUS LIPID EMULSIONS M.L. Forchielli1 , M. Conti2 , A. Stancari3 , S. Maselli3 , A.M. Guarguaglini3 , L. Loredana Bruno3 , D. Patrono2 , A. Pession1 , C. Puggioli3 , G. Bersani4 . 1 Pediatrics, university of Bologna Italy, 2 Analysis Laboratory, 3 Pharmacy Service, S Orsola-Malpighi Hosp, 4 Consulting Pharmacist, Bologna, Italy Rationale: No data exist for the vitamin A group and vitamin D2/D3 content in branded intravenous lipid emulsions (LEs). Our goal is to evaluate and quantify their concentrations in different LEs to assess whether they are clinically relevant. Methods: Analyses were carried out in triplicates on six LEs: 1) 30% soybean oil-based, 2) 20% olive-soybean oil based, 3) 10+10% soybean-medium-chain-triglyceride (MCT)
S233 coconut oil based, 4) 20% soybean-olive-MCT-fish-oil based, 5) 20% soybean-MCT-fish-oil based and 6) 10% pure fish oil based, respectively. Retinol and ergo-chole-calciferol were analyzed and quantified by a novel quali-quantitative Liquid Chromatography-Mass Spectrometry method after potassium hydroxide saponification, hexane extraction, reverse phase-liquid chromatography and specific multiple-residualmonitoring for retinol and vitamin D2/D3. The method proved to be extremely effective in term of sensitivity (3 ppb), specificity (mass spectrum), linearity (R2 = 0.99) and accuracy (CV <5%) for vitamin measurements in a complex lipid matrix. Statistics were provided by t-test. Results: Average retinol content was in the range of 0.2 1.0 mg/L in 1, 2, and 3 LEs, whereas it was higher (1 2 mg/L) in LEs containing fish-oil. Vitamin D content was in the range of 0.005 0.01 mg/L in fish-oil based LEs, but absent in those LEs containing purely vegetable oils. Conclusion: This study shows that retinol and vitamin D contents are variably present in LEs based on different lipid sources. These amounts need to be taken into account to provide correct intakes to patients. Disclosure of Interest: None Declared.
PP281-MON Outstanding abstract A RANDOMIZED CONTROLLED DOUBLE BLINDED CLINICAL TRIAL OF VITAMIN D3 SUPPLEMENTATION ON OBESITY PARAMETERS IN OBESE TYPE 2 DIABETES SUBJECTS IN THE UAE A. Sadiya1 . 1 Lifestyle Clinic, Rashid Centre for Diabetes and Research, Ajman, United Arab Emirates Rationale: The co-existence of vitamin D deficiency with obesity and type 2 diabetes is highly prevalent in this region. The objective of this study was to study the effect of Vitamin D3 supplementation on obesity parameters in obese Type 2 diabetes Emirati population in United Arab Emirates. Methods: A randomized double-blind clinical trial was conducted for 6 months with 87 vitamin D deficient obese, type 2 diabetic subjects. The study was divided into 3 phases; in Phase I the Vitamin D group (n = 45) received 6000IU Vitamin D3 /day (3 months), in Phase II 3000IU Vitamin D3/day or the placebo group (n = 42). During follow up both the arms were supplemented with 2200IU vitamin D3 /day for 6 months (Phase III). Results: At the baseline a significant (p < 0.01) positive association between body fat mass and body weight (r = 0.917) muscle mass (r = 0.47), water mass (r = 0.542), waist circumference (r = 0.815) and serum PTH (r = 0.284) was observed. On supplementation no significant changes were observed in body weight ( 0.8%, p = ns), BMI, waist circumference or fat mass ( 1.1%, p = ns). However, serum 25(OH)D peaked in phase I (28.5±9.2 vs 77.2±30.1, p = 0.003) followed by a decrease in phase II (61.4±18.8, p = 0.006), and consequently the PTH decreased in phase II (5.89±2.36 vs 5.89±2.36, p < 0.01) compared to baseline in vitamin D group. Conclusion: There is no significant influence of vitamin D supplementation on weight and fat loss in vitamin D deficient obese type 2 diabetic subjects of Arab ethnicity. However it normalized vitamin D status and reduced the incidence of eucalcemic PTH elevation. Disclosure of Interest: None Declared.