- Email: [email protected]
PPAR gamma activation can improve aberrant redox regulation in hypertension
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D. Rossin et al. / Free Radical Biology and Medicine 108 (2017) S18–S107
Solar ultraviolet (UV) radiation, especially UVB (290 nm-320 nm) component, causes DNA damage, pyrimidine dimmers, 8-hydroxy-2´-deoxyguanosine (8-OHdG), p53 induction, protein oxidation and generation of reactive oxygen species (ROS). Some polyphenols show the ability to protect the skin from the adverse effects of UVB radiation, including the risk of skin cancers. This study evaluated the protective effects of formulations containing citrus, rosemary and olive polyphenols (F1 and F2) against UVBinduced damage in human keratinocytes. F1 contained citrus, rosemary diterpenes and olive polyphenols and F2 citrus and olive flavones. The antioxidant capacity was determined using the ROS-sensitive dye 2’,7’-dichlorofluorescein diacetate (DCF-DA). Late apoptosis, mitochondrial membrane potential (MMP) and H2A.X histone phosphorylation were measured using Muse Cell Analyzer. The treatment with both formulations suppressed UVB-induced ROS production, and decreased late apoptosis, cell depolarization and DNA damage by H2A.X assay. In conclusion, these results suggest that the compounds present in both formulations might contribute to the observed protective effect. Formulations may be considered as candidates for oral or topical photoprotection and their mechanism of action may deserve further attention. E-mail address: [email protected] (N. Sánchez-Marzo) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.272
P-188
Antioxidant activity and photoprotective effect of citrus and olive formulations on UVB-induced damage in human keratinocytes Noelia Sánchez-Marzo 1, Almudena Pérez-Sánchez 1, Julián Castillo 2,3, María Herranz-López 1, Vicente Micol 1, Enrique Barrajón-Catalán 1 1
Molecular and Cell Biology Institute (IBMC), Miguel Hernández University (UMH), Avenida de la Universidad s/n, 03202, Elche, Alicante, Spain 2 Nutrafur S.A. Camino Viejo de Pliego, km.2, 30820, Alcantarilla, Murcia, Spain. Frutarom Group 3 Institute of Research into Aging, University of Murcia, 30100, Espinardo, Murcia, Spain
capacity. Both formulations also exhibited high protective effects on cell survival upon UVB radiation, finding a higher effect for F1 when both were used at same concentration. These results indicate that F1 may be considered as a better ingredient for oral or topical photoprotection. This suggests that, probably not only citrus and olive polyphenols but also rosemary compounds act in the protective action. E-mail address: [email protected] (N. Sánchez-Marzo) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.273
P-189
PPAR gamma activation can improve aberrant redox regulation in hypertension Ima Dovinova 1, Miroslava Kvandová 1, Miroslava Majzunova 1, Peter Balis 1, Linda Gresova 1, Miroslav Barancik 2 1
Institute of Normal and Pathological Physiology,Slovak Academy of Sciences,Bratislava, Slovak Republic 2 Institute for Heart Research, Slovak Academy of Sciences,Bratislava, Slovak Republic Keywords: PPAR gamma; Nrf2; redox regulation; hypertension
Dysregulation of redox- sensitive signaling plays an important role in develompment of hypertension. PPAR gamma belongs to nuclear receptors and is a potent nutritional sensor and redox regulator. In our study we focused on the role of PPAR gamma activation of RAS/ROS regulation, and antioxidant response in spontaneously hypertensive rats (SHR). Treatment was performed by gavage with PPAR gamma agonist pioglitazone (PIO - 10 mg/kg/ day, 10 days). Treatment with PPAR gamma agonist PIO in influenced blood pressure, redox- sensitive responses, lipid profile and homocystein level in age- dependent manner. The improvement parameters in hypertension and redox regulation have been observed in young animals, but not in adult SHR rats. PIO treatment influenced redox regulation (gene expression of Nrf2 and SOD isoforms) correlated with SOD a CAT activity and NO bioavailibility. PPAR gamma activation has been corelated with downregulation of and AT1R - RAS axis and „up-regulation“ of AT2R and Mas receptor. This most sensitive tissue responses in RAS/ROS signaling and NO level have been found primary in kidney of young hypertensive rats.
Keywords: Citrus; olive; rosemary; UVB radiation; antioxidant activity
E-mail address: [email protected] (I. Dovinova) UV radiation, absorbed by the epidermis, is the major cause of a variety of cutaneous disorders including photoageing and skin cancers. In recent years, an increase in the use of botanicals with antioxidant and anti-inflammatory properties as skin photoprotective agents is emerging. This study evaluated the antioxidant activity and the protective effect of citrus and olive formulations (F1 and F2), against UVB-induced damage in human keratinocytes. F1 contained citrus, rosemary diterpenes and olive polyphenols and F2 citrus and olive flavones. Total phenolic content was determined with Folin-Ciocalteu. The antioxidant activity was evaluated with different methods: trolox equivalent antioxidant capacity (TEAC), ferric reducing-antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Photoprotection study was performed determining the viability of HaCaT cells exposed to UVB through MTT assay. The F1 showed higher phenolic content and antioxidant
Acknowledgements
Supported by: APVV-0348-12, APVV-15–0565 and VEGA2/ 0148/17 http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.274
D. Rossin et al. / Free Radical Biology and Medicine 108 (2017) S18–S107
Solar ultraviolet (UV) radiation, especially UVB (290 nm-320 nm) component, causes DNA damage, pyrimidine dimmers, 8-hydroxy-2´-deoxyguanosine (8-OHdG), p53 induction, protein oxidation and generation of reactive oxygen species (ROS). Some polyphenols show the ability to protect the skin from the adverse effects of UVB radiation, including the risk of skin cancers. This study evaluated the protective effects of formulations containing citrus, rosemary and olive polyphenols (F1 and F2) against UVBinduced damage in human keratinocytes. F1 contained citrus, rosemary diterpenes and olive polyphenols and F2 citrus and olive flavones. The antioxidant capacity was determined using the ROS-sensitive dye 2’,7’-dichlorofluorescein diacetate (DCF-DA). Late apoptosis, mitochondrial membrane potential (MMP) and H2A.X histone phosphorylation were measured using Muse Cell Analyzer. The treatment with both formulations suppressed UVB-induced ROS production, and decreased late apoptosis, cell depolarization and DNA damage by H2A.X assay. In conclusion, these results suggest that the compounds present in both formulations might contribute to the observed protective effect. Formulations may be considered as candidates for oral or topical photoprotection and their mechanism of action may deserve further attention. E-mail address: [email protected] (N. Sánchez-Marzo) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.272
P-188
Antioxidant activity and photoprotective effect of citrus and olive formulations on UVB-induced damage in human keratinocytes Noelia Sánchez-Marzo 1, Almudena Pérez-Sánchez 1, Julián Castillo 2,3, María Herranz-López 1, Vicente Micol 1, Enrique Barrajón-Catalán 1 1
Molecular and Cell Biology Institute (IBMC), Miguel Hernández University (UMH), Avenida de la Universidad s/n, 03202, Elche, Alicante, Spain 2 Nutrafur S.A. Camino Viejo de Pliego, km.2, 30820, Alcantarilla, Murcia, Spain. Frutarom Group 3 Institute of Research into Aging, University of Murcia, 30100, Espinardo, Murcia, Spain
capacity. Both formulations also exhibited high protective effects on cell survival upon UVB radiation, finding a higher effect for F1 when both were used at same concentration. These results indicate that F1 may be considered as a better ingredient for oral or topical photoprotection. This suggests that, probably not only citrus and olive polyphenols but also rosemary compounds act in the protective action. E-mail address: [email protected] (N. Sánchez-Marzo) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.273
P-189
PPAR gamma activation can improve aberrant redox regulation in hypertension Ima Dovinova 1, Miroslava Kvandová 1, Miroslava Majzunova 1, Peter Balis 1, Linda Gresova 1, Miroslav Barancik 2 1
Institute of Normal and Pathological Physiology,Slovak Academy of Sciences,Bratislava, Slovak Republic 2 Institute for Heart Research, Slovak Academy of Sciences,Bratislava, Slovak Republic Keywords: PPAR gamma; Nrf2; redox regulation; hypertension
Dysregulation of redox- sensitive signaling plays an important role in develompment of hypertension. PPAR gamma belongs to nuclear receptors and is a potent nutritional sensor and redox regulator. In our study we focused on the role of PPAR gamma activation of RAS/ROS regulation, and antioxidant response in spontaneously hypertensive rats (SHR). Treatment was performed by gavage with PPAR gamma agonist pioglitazone (PIO - 10 mg/kg/ day, 10 days). Treatment with PPAR gamma agonist PIO in influenced blood pressure, redox- sensitive responses, lipid profile and homocystein level in age- dependent manner. The improvement parameters in hypertension and redox regulation have been observed in young animals, but not in adult SHR rats. PIO treatment influenced redox regulation (gene expression of Nrf2 and SOD isoforms) correlated with SOD a CAT activity and NO bioavailibility. PPAR gamma activation has been corelated with downregulation of and AT1R - RAS axis and „up-regulation“ of AT2R and Mas receptor. This most sensitive tissue responses in RAS/ROS signaling and NO level have been found primary in kidney of young hypertensive rats.
Keywords: Citrus; olive; rosemary; UVB radiation; antioxidant activity
E-mail address: [email protected] (I. Dovinova) UV radiation, absorbed by the epidermis, is the major cause of a variety of cutaneous disorders including photoageing and skin cancers. In recent years, an increase in the use of botanicals with antioxidant and anti-inflammatory properties as skin photoprotective agents is emerging. This study evaluated the antioxidant activity and the protective effect of citrus and olive formulations (F1 and F2), against UVB-induced damage in human keratinocytes. F1 contained citrus, rosemary diterpenes and olive polyphenols and F2 citrus and olive flavones. Total phenolic content was determined with Folin-Ciocalteu. The antioxidant activity was evaluated with different methods: trolox equivalent antioxidant capacity (TEAC), ferric reducing-antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Photoprotection study was performed determining the viability of HaCaT cells exposed to UVB through MTT assay. The F1 showed higher phenolic content and antioxidant
Acknowledgements
Supported by: APVV-0348-12, APVV-15–0565 and VEGA2/ 0148/17 http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.274