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Practical methods in molecular biology
~- ‘625
&efect~eniist~~&d Biqenerget
.. : ., .I :%A_, :..-..;.is;usa~e~-~ . . . ..‘. ;F&ted ...’ ,.in, Tiie Nethedtids .~ .::- .,T . -&-& re~em.;fc; _’ .. ; :_: --. y.: .: .:I.._ ..‘I_ z.. : .._ .~.: _:_,-. : ~~ra~tica~:~h&~ds~ in‘ Motecglar. Biology. -RF. Schleif. .Verla& .Neg Y&k Heidelberg, Berlin, 1981, -xiii + .. ._ ., : : _... ..
..
::.
1. tid P.C. Wensink. Springer220 pp. QM69.00, US$32.20.
In this laboratory-m&h&l book a number of techniques used in molecular biology a& collect&&’ Th.ere..are two. chapters oti- growth and use of E. coli and phage, includjng.some basic genetics, and one describing a few enzyme assays as.examples. Two..c@apter& arti-devoted to the I¶ti~n and analysis of proteins and nucleic acids, +cludirig most .of .the chromato&?phic and electrophoretic methods generally used. One chapter describes methods for the construction of recombinant DNA molecules, E. &oli $&formation, screening recombinant DNA clones, radio-labelling of DNA and RNA, Southern blots and hybridization and in uitro translation systems. Finalli some useful assorted laboratory techniques are reported. In general, only one or very few of the possible .altemative‘ procedures are given, but they we highly reliable. Moreover, some important techniques for example DNA sequencing, have been completely omitted, considering the fact that collections of these can be Found in other publications. Thus, the reader should not expect to find an answer to every problem, but he can expect to find a large amount of useful information including many unorthodox laboratory methods which are seldom found in printed form. This book can be useful in any molecular
biology laboratory,
and it can be particularly recommended to those entering the field as students at the
beginning of their labor&tory experience. F. AMALDI Rome
Ions, Etectrodes and Membranes. J. Koryta. John Wiley St Sons, Chichester, New York, Brisbane, Toronto, Singapore, 1982, viii + 197 pages, f 17.00, US$39.60. This book is an introduction to el&trochemistry destined particularly for students cdming from other fields of science. It explains i& a simple physico-chemical manner the basic concepts and applications of el&trochemistry, also giving to the reader a fairly precise picture of ionic solutions and membrane structurti and behaviour. The author d&scribes.a b&c experiment when introducing a problem and builds upon this to. f&muIate a general law jnd ~discu& i(s cons&quences. Static and transport properties of elcctrcjlytd sqlutions are described in the first chapter. I& second cba$er’ de& with electrodes in equilibrium with iheir surroundings, .a.4~wey .a$ with~@t~&- and mechanisms of @e&rode processes including applic&ions to technoldgjr *fi to chemical_ +d biochemical amjlysis. The.. third chabte; is .d&ot* tbl.el&rocheticz& artificial ti,d ._bio!ogical membranks, and to : .. .’ ..: ..
&efect~eniist~~&d Biqenerget
.. : ., .I :%A_, :..-..;.is;usa~e~-~ . . . ..‘. ;F&ted ...’ ,.in, Tiie Nethedtids .~ .::- .,T . -&-& re~em.;fc; _’ .. ; :_: --. y.: .: .:I.._ ..‘I_ z.. : .._ .~.: _:_,-. : ~~ra~tica~:~h&~ds~ in‘ Motecglar. Biology. -RF. Schleif. .Verla& .Neg Y&k Heidelberg, Berlin, 1981, -xiii + .. ._ ., : : _... ..
..
::.
1. tid P.C. Wensink. Springer220 pp. QM69.00, US$32.20.
In this laboratory-m&h&l book a number of techniques used in molecular biology a& collect&&’ Th.ere..are two. chapters oti- growth and use of E. coli and phage, includjng.some basic genetics, and one describing a few enzyme assays as.examples. Two..c@apter& arti-devoted to the I¶ti~n and analysis of proteins and nucleic acids, +cludirig most .of .the chromato&?phic and electrophoretic methods generally used. One chapter describes methods for the construction of recombinant DNA molecules, E. &oli $&formation, screening recombinant DNA clones, radio-labelling of DNA and RNA, Southern blots and hybridization and in uitro translation systems. Finalli some useful assorted laboratory techniques are reported. In general, only one or very few of the possible .altemative‘ procedures are given, but they we highly reliable. Moreover, some important techniques for example DNA sequencing, have been completely omitted, considering the fact that collections of these can be Found in other publications. Thus, the reader should not expect to find an answer to every problem, but he can expect to find a large amount of useful information including many unorthodox laboratory methods which are seldom found in printed form. This book can be useful in any molecular
biology laboratory,
and it can be particularly recommended to those entering the field as students at the
beginning of their labor&tory experience. F. AMALDI Rome
Ions, Etectrodes and Membranes. J. Koryta. John Wiley St Sons, Chichester, New York, Brisbane, Toronto, Singapore, 1982, viii + 197 pages, f 17.00, US$39.60. This book is an introduction to el&trochemistry destined particularly for students cdming from other fields of science. It explains i& a simple physico-chemical manner the basic concepts and applications of el&trochemistry, also giving to the reader a fairly precise picture of ionic solutions and membrane structurti and behaviour. The author d&scribes.a b&c experiment when introducing a problem and builds upon this to. f&muIate a general law jnd ~discu& i(s cons&quences. Static and transport properties of elcctrcjlytd sqlutions are described in the first chapter. I& second cba$er’ de& with electrodes in equilibrium with iheir surroundings, .a.4~wey .a$ with~@t~&- and mechanisms of @e&rode processes including applic&ions to technoldgjr *fi to chemical_ +d biochemical amjlysis. The.. third chabte; is .d&ot* tbl.el&rocheticz& artificial ti,d ._bio!ogical membranks, and to : .. .’ ..: ..