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Preferential synthesis of β-galactosidase after amino acid starvation
Vol. 9, No. 1, 1962
BIOCHEMICAL
PREFERENTU
SYNTHESIS
AFTER
Department Received
July
27,
sometimes
this
reported --E.
sidase,
coli
acids
gene
K-12
(Fig.
both
a negative
preferential
minutes
*U.
in the absence
1961).
synthesis
1962).
synthesis strain,
total such
starvation.
Service
Training
Fellow 84
of
by a single
was
of this observed
of a carbon
starvation are
if a
starved
for
is synthesized
even
In the inducible
strain,
was observed
of @-galactosidase
supply
the synthesis
methionine
the enzyme
rate.
mutant
strain,
if the cells
did not show
of C14-leucine,
p-galacto-
and the absence inducible
6, Yanagisawa
preferential
for
synthesis
starvation; source,
high
is controlled
of p-galactosidase
hthe
at very
In a constitutive
after
at unique
conditions.
of an exogenous
character
repressed
In the constitutive
Health
This
Ln the original
during
1, Curve
of methionine
S. Public
City
increase
abnormal
and inducible
in the presence
and a carbon
therefore,
This
rate
5).
is present
(Fig.
by the uptake
even
is strongly
methionine
restored.
York
cell
is synthesized
and Brenner,
1, Curve
source
under
methionineless
starvation
starvation
was
New
of a bacterial
p -galactosidase
initial
of b-galactosidase carbon
STARVATION
is broken
--et al , 1955).
high
methionine
source
balance
RNA
(Borek
a very
after
p-GALACTOSIDASE
Keiko Yanagis awa* Columbia University,
58-161,
caJ.led -RC (Stent
strain,
ACID
constituents
here,
synthesizes
ammo
OF
RESEARCH COMMUNICATIONS
1962
medium,
system
AMINO
of Zoology,
In a given rates;
AND BIOPHYSICAL
protein
during
when
methionine
synthesis,
studied
an abnormal is observed
behavior even
after
(Fig. 10
2).
BIOCHEMICAL
Vol. 9, No. 1, 1962
AND BIOPHYSICAL
RESEARCH COMMUNICATIONS
-STARVATION
0
30
60 TIME
90
I20
150
IN MINUTES
synthesis of B-galactosidase after Fig. 1, Preferential methionine starvation. Curves 1-4. control: glycerol and methionine were present The inducer IPTG was given to the inducible from 0 time. cells at 0 time. 1. E. coli (K-12) 58-161, constitutive. 2. k. coli (K-12) 58-161, inducible. 3. --E. coli 15, his- met-, inducible. 4. --E. coli (K-12) Ya-2, inducible. Starved for methionine and glycerol in the Curves 5-8. presence of IPTG. 5. --E. coli (K-12) 58-161, constitutive. 6. --E. coli (K-12) 58-161, inducible. 7. --E. coli 15, his’ met: 8, --E. co-ii (K-lTYa-2. As mentioned amount
of RNA
histidineless, RNA
after
1, curve
fl-galactosidase
the two seem
upon whether
strains
to favor
strain
60 minutes
starvation.
controlled 7, there
restoration
the difference
or by a difference the former
synthesizes
was
for
acids,
no preferential
is caused in inducibility.
a5
80% of the normal E. --
coli
p-galactosidase,
by ammo
of methionine.
view.
about
As a control,
and inducible
is stringently
in Figure
is not clear
this
methionineless
synthesis
shown
before,
in which
was used. synthesis
From
these
by a different The
15,
of results,
RC locus -
following
As
facts
it in
Vol. 9, No. 1, 1962
BIOCHEMICAL
AND BIOPHYSICAL
RESEARCH COMMIJNICATIONS
ro,ooo -
Y sP
METHIONINE
3
2 3
-STARVATION 5,000
AND
GLYCEROL
ADDED
-
s i 0
TIME
IN
MINUTES
uptake by E. coli (K-12) 58-161, Fig. 2, C14- leucine constitutive for fi -galactosidase. 1. Control: glycerol and methionine were present from 0 time. 2. Starved for methionine and glycerol for 60 minutes.
--E. coli which
sidase, 161 when Curve was sence
K-12
8).
After
deprived
at a high
of inducer
during with
this
preferential
state
of -RC locus
during
starvation,
accumulate.
and Brenner
of both
rate
58-161.
by amino amount
but not a direct since
p-galacto-
(1961) upon
In Ya-2,
the synthesis
of RNA
in 58-161.
after
relation
in the absence
has
of RNA
The
former
is more synthesizes
of methionine
Therefore, some
smaller
it would
relation
to the accumulation of a carbon
1,
or ab-
is much
60 minutes
in 58-161.
of the enzyme
(Fig.
of the presence the rate
than
58-
0 -galactosidase
However,
acids
crossing
and glycerol
starvation,
regardless
of the 80% observed synthesis
for
methionine
of methionine initial
coli
40% of the normal instead
and inducible
starvation.
E. --
controlled
starvation that
were
by Stent
60 minutes
synthesized
stringently
methionineless
was obtained
the cells
in comparison
about
Ya-2,
source
to the of RNA
RNA
did not
seem
BIOCHEMICAL
Vol. 9, No. 1, 1962
AND BIOPHYSICAL
RESEARCH COMMUNICATIONS
Acknowledgement The for
author
his guidance
by grants Health
from
wishes
to express
her
and encouragement. the National
thanks This
Science
to Professor
work
Foundation
F.
was supported and the U.
J. Ryan in part
S. Public
Service. References
Borek, Stent,
E.,
Rockenbach,
G. S. and Brenner,
Yanagisawa,
K. , Biochem.
J. and Ryan
A.,
S. , Proc.
Natl.
Acad.
Biophys.
Res.
Corn.
87
J. Bacterial.
11 , 318 (1955)
Sci. ,
2,
LT. S. -47, 88
(1962)
2005
(1961)
BIOCHEMICAL
PREFERENTU
SYNTHESIS
AFTER
Department Received
July
27,
sometimes
this
reported --E.
sidase,
coli
acids
gene
K-12
(Fig.
both
a negative
preferential
minutes
*U.
in the absence
1961).
synthesis
1962).
synthesis strain,
total such
starvation.
Service
Training
Fellow 84
of
by a single
was
of this observed
of a carbon
starvation are
if a
starved
for
is synthesized
even
In the inducible
strain,
was observed
of @-galactosidase
supply
the synthesis
methionine
the enzyme
rate.
mutant
strain,
if the cells
did not show
of C14-leucine,
p-galacto-
and the absence inducible
6, Yanagisawa
preferential
for
synthesis
starvation; source,
high
is controlled
of p-galactosidase
hthe
at very
In a constitutive
after
at unique
conditions.
of an exogenous
character
repressed
In the constitutive
Health
This
Ln the original
during
1, Curve
of methionine
S. Public
City
increase
abnormal
and inducible
in the presence
and a carbon
therefore,
This
rate
5).
is present
(Fig.
by the uptake
even
is strongly
methionine
restored.
York
cell
is synthesized
and Brenner,
1, Curve
source
under
methionineless
starvation
starvation
was
New
of a bacterial
p -galactosidase
initial
of b-galactosidase carbon
STARVATION
is broken
--et al , 1955).
high
methionine
source
balance
RNA
(Borek
a very
after
p-GALACTOSIDASE
Keiko Yanagis awa* Columbia University,
58-161,
caJ.led -RC (Stent
strain,
ACID
constituents
here,
synthesizes
ammo
OF
RESEARCH COMMUNICATIONS
1962
medium,
system
AMINO
of Zoology,
In a given rates;
AND BIOPHYSICAL
protein
during
when
methionine
synthesis,
studied
an abnormal is observed
behavior even
after
(Fig. 10
2).
BIOCHEMICAL
Vol. 9, No. 1, 1962
AND BIOPHYSICAL
RESEARCH COMMUNICATIONS
-STARVATION
0
30
60 TIME
90
I20
150
IN MINUTES
synthesis of B-galactosidase after Fig. 1, Preferential methionine starvation. Curves 1-4. control: glycerol and methionine were present The inducer IPTG was given to the inducible from 0 time. cells at 0 time. 1. E. coli (K-12) 58-161, constitutive. 2. k. coli (K-12) 58-161, inducible. 3. --E. coli 15, his- met-, inducible. 4. --E. coli (K-12) Ya-2, inducible. Starved for methionine and glycerol in the Curves 5-8. presence of IPTG. 5. --E. coli (K-12) 58-161, constitutive. 6. --E. coli (K-12) 58-161, inducible. 7. --E. coli 15, his’ met: 8, --E. co-ii (K-lTYa-2. As mentioned amount
of RNA
histidineless, RNA
after
1, curve
fl-galactosidase
the two seem
upon whether
strains
to favor
strain
60 minutes
starvation.
controlled 7, there
restoration
the difference
or by a difference the former
synthesizes
was
for
acids,
no preferential
is caused in inducibility.
a5
80% of the normal E. --
coli
p-galactosidase,
by ammo
of methionine.
view.
about
As a control,
and inducible
is stringently
in Figure
is not clear
this
methionineless
synthesis
shown
before,
in which
was used. synthesis
From
these
by a different The
15,
of results,
RC locus -
following
As
facts
it in
Vol. 9, No. 1, 1962
BIOCHEMICAL
AND BIOPHYSICAL
RESEARCH COMMIJNICATIONS
ro,ooo -
Y sP
METHIONINE
3
2 3
-STARVATION 5,000
AND
GLYCEROL
ADDED
-
s i 0
TIME
IN
MINUTES
uptake by E. coli (K-12) 58-161, Fig. 2, C14- leucine constitutive for fi -galactosidase. 1. Control: glycerol and methionine were present from 0 time. 2. Starved for methionine and glycerol for 60 minutes.
--E. coli which
sidase, 161 when Curve was sence
K-12
8).
After
deprived
at a high
of inducer
during with
this
preferential
state
of -RC locus
during
starvation,
accumulate.
and Brenner
of both
rate
58-161.
by amino amount
but not a direct since
p-galacto-
(1961) upon
In Ya-2,
the synthesis
of RNA
in 58-161.
after
relation
in the absence
has
of RNA
The
former
is more synthesizes
of methionine
Therefore, some
smaller
it would
relation
to the accumulation of a carbon
1,
or ab-
is much
60 minutes
in 58-161.
of the enzyme
(Fig.
of the presence the rate
than
58-
0 -galactosidase
However,
acids
crossing
and glycerol
starvation,
regardless
of the 80% observed synthesis
for
methionine
of methionine initial
coli
40% of the normal instead
and inducible
starvation.
E. --
controlled
starvation that
were
by Stent
60 minutes
synthesized
stringently
methionineless
was obtained
the cells
in comparison
about
Ya-2,
source
to the of RNA
RNA
did not
seem
BIOCHEMICAL
Vol. 9, No. 1, 1962
AND BIOPHYSICAL
RESEARCH COMMUNICATIONS
Acknowledgement The for
author
his guidance
by grants Health
from
wishes
to express
her
and encouragement. the National
thanks This
Science
to Professor
work
Foundation
F.
was supported and the U.
J. Ryan in part
S. Public
Service. References
Borek, Stent,
E.,
Rockenbach,
G. S. and Brenner,
Yanagisawa,
K. , Biochem.
J. and Ryan
A.,
S. , Proc.
Natl.
Acad.
Biophys.
Res.
Corn.
87
J. Bacterial.
11 , 318 (1955)
Sci. ,
2,
LT. S. -47, 88
(1962)
2005
(1961)