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Preparation and in vitro evaluation of sulforaphane intragastric buoyant sustained-release tablets
Abstracts / Journal of Biotechnology 136S (2008) S22–S71
S53
I1-P-090
References
Application of response surface methodology to the production of polysaccharide by Agrobacterium sp
Kueh, R., Rahman, N.A., Merican, A.F., 2003. Computational docking of l-arginine and its structural analogues to C-terminal domain of Escherichia coli arginine repressor protein (ArgRc). J. Mol. Model. 9, 88–98. Ma karova K.S., Mironov, Gelfand, M.S., 2001. Conservation of the binding site for the arginine repressor in all bacterial lineages. Genome Biol. 2: research0013.10013.8. Naihu, W., 2002. Principle of Gene Engineering. Science Publishing Ltd, Beijing. Sakanyan, V., Petrosyan, P., Lecocq, M., Boven, A., Legrain, C., Demarez, M., Hallet, J.N., Glansdorff, N., 1996. Genes and enzymes of the acetyl cycle of arginine biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early steps of the arginine pathway. Microbiology 142, 99–108.
Ing-Lung Shih 1,∗ , Jin-Yee Yu 2 , Jane-Yii Wu 2 1
Department of Environmental Engineering, Da-Yeh University, Chang-Hwa, Taiwan 2 Department of Bioindustry Technology, Da-Yeh University, ChangHwa, Taiwan Agrobacterium sp. was studied for the production of curdlan, beta(1, 3)-d-glucans, by response surface methodology (RSM). Several operational and nutritional factors, such as initial pH, urea concentration, sucrose concentration, having the greatest influence on the curdlan production were identified. The curdlan production by Agrobacterium sp. was increased significantly when the strain was cultivated in the optimal medium developed by RSM as compared to conventional one-factor-at-a-time technique. The curdlan production rate of 0.88 g/l/h was obtained when Agrobacterium sp. was cultivated in the optimal medium developed by RSM, which was the highest curdlan production rate reported to date. The infrared (IR) and NMR spectra, the thermogram of DSC and pattern of Xray diffraction for the curdlan of the present study were almost identical to those of the authentic sample (from Sigma). The purified curdlan was a linear polysaccharide composed of exclusively -(1,3)-glucosidic linkages with the molecular weight of 155 kDa by GPC. Keywords: Response surface methodology; Curdlan; Beta-(1, 3)-dglucans; Agrobacterium sp.; Central composite design doi:10.1016/j.jbiotec.2008.07.111 I1-P-091 Isolation and molecular analysis of the gene cluster for the arginine biosynethetic gene from Corynebacterium crenatum Yonghua Xiong 1 , Xuelan Chen 2,∗ , Wenyi Tao 3 1
Sino-Germany Joint Research Institute, Nanchang University, Nanjing Donglu 235, Nanchang 330047, China 2 Bioscience School, Jiangxi Normal University, Ziyang Road 99, Nanchang 330022, China 3 Biotechnology school, Jiangnan University, Lihu Road 1800, Wuxi 214122, China E-mail address: [email protected] (X. Chen). In order to clarify the characteristics of the arginine biosynthetic genes of C. crenatum AS1.542, arginine biosynethetic gene cluster, containing argJ, argB, argD, argF and argR as well as partial argC, had been amplified and sequenced. The gene order argCJBDFR had been arranged by alignment the entire 6.08-kb DNA fragment. The four ORFs, argBDFR, locate in the same transcription unit. The transcript termination of argC gene is irrelevant with the rhofactor. Comparison with ArgJ polypeptide of C. glutamate, ornithine acetyltransferases from C. crenatum also belongs to monofunctional enzyme. The evolutionary implications of these data are discussed. Keywords: Arginine; Corynebacterium crenatum; Ornithine acetyltransferases; ArgCJBDFR
∗ Corresponding author. Tel.: +86 791 8120391; fax: +86 791 8112327.
doi:10.1016/j.jbiotec.2008.07.112 I1-P-092 Preparation and in vitro evaluation of sulforaphane intragastric buoyant sustained-release tablets Qipeng Yuan ∗ , Xiaodan Hou, Hao Liang, Chunfang Li Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, PR China An intragastric buoyant sustained-release tablet containing 30 mg sulforaphane (SFN) (Fahey et al., 2002; Fimognari et al., 2005; Shapiro et al., 2006) that used to eradicate gastric Helicobacter pylori was prepared by using hydroxypropyl methylcellulose K4M (HPMC K4M) and polyvinylpyrrolidone (PVP K30) as excipients. Several formulations were investigated, which were with different ratios of HPMC to PVP and without changing the content of sulforaphane. During the in vitro release study all the tablets could achieve floatation immediately when they were put into water and keep afloat for at least 12 h. But tablet compositions have great influence on drug release. When the ratio of HPMC to PVP decreased, the tablet showed a faster release profile. The formulation with 45% PVP not only prolonged the residence time of the chemical active ingredient in the stomach, but also reached the minimum inhibition concentration of the drug and sustained this concentration for 12 h. Moreover the release exponent n of this formulation was 0.6949, which indicated the non-Fickian diffusion (Ritger and Peppas, 1987; Rothenbacher and Brenner, 2003) occurred. Keywords: Sulforaphane; Helicobacter pylori; Sustained-release References Fahey, J.W., Haristoy, X., Dolan, P.M., Kensler, T.W., Scholtus, I., Stephenson, K.K., Talalay, P., Lozniewski, A., 2002. Sulforaphane inhibits extracellular, intracellular and antibiotic-resistant strains of Helicobacter pylori and prevents benzo(␣) pyrene-induced stomach tumors. Proc. Natl. Acad. Sci. 99, 7610–7615. Fimognari, C., Berti, F., Iori, R., Cantelli-Forti, G., Hrelia, P., 2005. Micronucleus formation and induction of apoptosis by different isothiocyanates and a mixture of isothiocyanates in human lymphocyte cultures. Mutat. Res. 582, 1–10. Ritger, P.L., Peppas, N.A., 1987. A simple equation for description of solute release. II. Fickian and anomalous release from swellable devices. J. Control Release 5, 37–42. Rothenbacher, D., Brenner, H., 2003. Burden of Helicobacter pylori and H. pylorirelated diseases in developed countries: recent developments and future implications. Microbes. Infect. 5, 693–703. Shapiro, T.A., Fahey, J.W., Dinkova-Kostova, A.T., Holtzclaw, W.D., Stephenson, K.K., Wade, K.L., Ye, L., Talalay, P., 2006. Safety, tolerance, and metabolism of broccoli sprout glucosinolates and isothiocyanates: a clinical phase I study. Nutr. Cancer 55, 53–62.
doi:10.1016/j.jbiotec.2008.07.113
S53
I1-P-090
References
Application of response surface methodology to the production of polysaccharide by Agrobacterium sp
Kueh, R., Rahman, N.A., Merican, A.F., 2003. Computational docking of l-arginine and its structural analogues to C-terminal domain of Escherichia coli arginine repressor protein (ArgRc). J. Mol. Model. 9, 88–98. Ma karova K.S., Mironov, Gelfand, M.S., 2001. Conservation of the binding site for the arginine repressor in all bacterial lineages. Genome Biol. 2: research0013.10013.8. Naihu, W., 2002. Principle of Gene Engineering. Science Publishing Ltd, Beijing. Sakanyan, V., Petrosyan, P., Lecocq, M., Boven, A., Legrain, C., Demarez, M., Hallet, J.N., Glansdorff, N., 1996. Genes and enzymes of the acetyl cycle of arginine biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early steps of the arginine pathway. Microbiology 142, 99–108.
Ing-Lung Shih 1,∗ , Jin-Yee Yu 2 , Jane-Yii Wu 2 1
Department of Environmental Engineering, Da-Yeh University, Chang-Hwa, Taiwan 2 Department of Bioindustry Technology, Da-Yeh University, ChangHwa, Taiwan Agrobacterium sp. was studied for the production of curdlan, beta(1, 3)-d-glucans, by response surface methodology (RSM). Several operational and nutritional factors, such as initial pH, urea concentration, sucrose concentration, having the greatest influence on the curdlan production were identified. The curdlan production by Agrobacterium sp. was increased significantly when the strain was cultivated in the optimal medium developed by RSM as compared to conventional one-factor-at-a-time technique. The curdlan production rate of 0.88 g/l/h was obtained when Agrobacterium sp. was cultivated in the optimal medium developed by RSM, which was the highest curdlan production rate reported to date. The infrared (IR) and NMR spectra, the thermogram of DSC and pattern of Xray diffraction for the curdlan of the present study were almost identical to those of the authentic sample (from Sigma). The purified curdlan was a linear polysaccharide composed of exclusively -(1,3)-glucosidic linkages with the molecular weight of 155 kDa by GPC. Keywords: Response surface methodology; Curdlan; Beta-(1, 3)-dglucans; Agrobacterium sp.; Central composite design doi:10.1016/j.jbiotec.2008.07.111 I1-P-091 Isolation and molecular analysis of the gene cluster for the arginine biosynethetic gene from Corynebacterium crenatum Yonghua Xiong 1 , Xuelan Chen 2,∗ , Wenyi Tao 3 1
Sino-Germany Joint Research Institute, Nanchang University, Nanjing Donglu 235, Nanchang 330047, China 2 Bioscience School, Jiangxi Normal University, Ziyang Road 99, Nanchang 330022, China 3 Biotechnology school, Jiangnan University, Lihu Road 1800, Wuxi 214122, China E-mail address: [email protected] (X. Chen). In order to clarify the characteristics of the arginine biosynthetic genes of C. crenatum AS1.542, arginine biosynethetic gene cluster, containing argJ, argB, argD, argF and argR as well as partial argC, had been amplified and sequenced. The gene order argCJBDFR had been arranged by alignment the entire 6.08-kb DNA fragment. The four ORFs, argBDFR, locate in the same transcription unit. The transcript termination of argC gene is irrelevant with the rhofactor. Comparison with ArgJ polypeptide of C. glutamate, ornithine acetyltransferases from C. crenatum also belongs to monofunctional enzyme. The evolutionary implications of these data are discussed. Keywords: Arginine; Corynebacterium crenatum; Ornithine acetyltransferases; ArgCJBDFR
∗ Corresponding author. Tel.: +86 791 8120391; fax: +86 791 8112327.
doi:10.1016/j.jbiotec.2008.07.112 I1-P-092 Preparation and in vitro evaluation of sulforaphane intragastric buoyant sustained-release tablets Qipeng Yuan ∗ , Xiaodan Hou, Hao Liang, Chunfang Li Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, PR China An intragastric buoyant sustained-release tablet containing 30 mg sulforaphane (SFN) (Fahey et al., 2002; Fimognari et al., 2005; Shapiro et al., 2006) that used to eradicate gastric Helicobacter pylori was prepared by using hydroxypropyl methylcellulose K4M (HPMC K4M) and polyvinylpyrrolidone (PVP K30) as excipients. Several formulations were investigated, which were with different ratios of HPMC to PVP and without changing the content of sulforaphane. During the in vitro release study all the tablets could achieve floatation immediately when they were put into water and keep afloat for at least 12 h. But tablet compositions have great influence on drug release. When the ratio of HPMC to PVP decreased, the tablet showed a faster release profile. The formulation with 45% PVP not only prolonged the residence time of the chemical active ingredient in the stomach, but also reached the minimum inhibition concentration of the drug and sustained this concentration for 12 h. Moreover the release exponent n of this formulation was 0.6949, which indicated the non-Fickian diffusion (Ritger and Peppas, 1987; Rothenbacher and Brenner, 2003) occurred. Keywords: Sulforaphane; Helicobacter pylori; Sustained-release References Fahey, J.W., Haristoy, X., Dolan, P.M., Kensler, T.W., Scholtus, I., Stephenson, K.K., Talalay, P., Lozniewski, A., 2002. Sulforaphane inhibits extracellular, intracellular and antibiotic-resistant strains of Helicobacter pylori and prevents benzo(␣) pyrene-induced stomach tumors. Proc. Natl. Acad. Sci. 99, 7610–7615. Fimognari, C., Berti, F., Iori, R., Cantelli-Forti, G., Hrelia, P., 2005. Micronucleus formation and induction of apoptosis by different isothiocyanates and a mixture of isothiocyanates in human lymphocyte cultures. Mutat. Res. 582, 1–10. Ritger, P.L., Peppas, N.A., 1987. A simple equation for description of solute release. II. Fickian and anomalous release from swellable devices. J. Control Release 5, 37–42. Rothenbacher, D., Brenner, H., 2003. Burden of Helicobacter pylori and H. pylorirelated diseases in developed countries: recent developments and future implications. Microbes. Infect. 5, 693–703. Shapiro, T.A., Fahey, J.W., Dinkova-Kostova, A.T., Holtzclaw, W.D., Stephenson, K.K., Wade, K.L., Ye, L., Talalay, P., 2006. Safety, tolerance, and metabolism of broccoli sprout glucosinolates and isothiocyanates: a clinical phase I study. Nutr. Cancer 55, 53–62.
doi:10.1016/j.jbiotec.2008.07.113