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Preparative centrifugation; a practical approach
ht. J. Biochem.Vol. 25, No. 12, pp. 1981-1982, 1993 Printed in Great Britain
Pergamon Press Ltd.
BOOK REVIEWS
Gene Transcription; A Practical Approach-Edited by B. D. HAME~ and S. J. HIGGINS. 364~~. 1993. IRL/Oxford University Press, Oxford. HB $60, PB $42.
Preparative Centrifugatioo; by D. RICKW~~D. 399~~. Press, Oxford. PB f25.
The initial advances were made in the study of the DNA, RNA and transcription in Escherichiu co/i and viruses. Now there have been considerable advances in the study of transcription in the eukaryote with the discovery of three distinct RNA polymerases, techniques of cloning, mutagenesis, transfection, domain mapping, promoters and enhancers. This book concentrates on RNA polymerase II. It gives practical details how to deal with: assay of gene transcription in vitro; transcription of endogenous genes in mammalian cells; in vitro transcription with nuclear extracts from differentiated tissues; trangeneic animals; eukaryotic transcription factors; protein-DNA interactions; cloning and functional analysis of heterologous eukaryotic transcription factors in yeast; transcription controls, cis-elements and rrans-factors; fractionation of nucleic acids by gel electrophoresis.
The development in instrumentation and better control systems of centrifuges has allowed the more accurate and precise separation of specific identified fractions of the cell. The days are almost gone when you had to have the powerful centrifuge placed in a shed outside some way away, in case the rotor exploded and danced through the walls like a mis-guided missile. This book gives the theory and practical details how to obtain the best conditions for sub cellular separations. It deals with: theoretical aspects of practical centrifugation (differential pelleting, gradient separations; isopychnic; self forming gradients); centrifuges and rotors; conditions for density gradient separations; characterization of macromolecules and their interactions; measurement of sedimentation coefficients; isolation and characterization of membrane and cell organelles; separation of mammalian cells; separation in zonal rotors; specification of ultracentrifuge rotors; marker enzymes and chemical assays of subcellular fractions.
Protein Engineering; A Practical Approach-Edited by A. R. REEX, M. J. E. STERNBERGand R. WETZEL. 397~~. 1992. IRL/Oxford University Press, Oxford. PB E25.
A Practical Approach--Edited 1992. IRL/Oxford University
Gastrointestinal Regulatory PeptidesEdited by D. R. BROWN. Handbook of Experimental Pharmacology, Vol. 106. 446~~. 1993. Springer Verlag, Berlin. DM 480.
The problem is to produce a specific purified protein in significant quantities. This book provides some practical details that can help in this task. The chapters deal with: structural analysis of proteins, (a) protein crystallography, (b) NMR; molecular sequence data bases and their uses; design of protein structures; from protein sequence to structure; theoretical methods in protein engineering; protein stability, (a) reversible unfolding, kinetic and thermodynamic analysis, (b) enhanced folding, stabilization suppression of aggregation in oitro and in uivo; protein turnover-possible manipulations; chemical approaches to protein engineering; antibody combining sites; display of antibodies and proteins on the surface of bacteriophage fd; expression of proteins in prokaryotes; expression of proteins in yeast; genetic engineering of tumor necrosis factor; production of alpha I-antitrypsin-a case study.
The GI regulatory peptides (GIRP) have received increased interest since it was realized that many GIRP have regulatory roles in the CNS and other body systems. This book deals with: the localization and co-localization of GIRP; regulation of GIRP gene expression; post translation processing of GIRP, regulation of peptide secretion of endocrine cells; peptide receptors and signal transduction; proteolytic inactivation of GIRP; peptidergic regulation of acid secretion; peptides and enteric neural activity; GIRP regulation of smooth muscle contractility; regulation of intestinal electrolyte transport; regulation of GI blood flow; regulation of cell proliferation through multiple signalling pathways; mucosal immune function; pathophysiology of GIRP; GIRP as therapeutic agents. The reviews are of a high standard, as to be expected of this series.
Lipid Analysis; A Practical ApproacbEdited by R. J. HAMILTON and S. HAMILTON. 310~~. 1992. IRL/Oxford University Press, Oxford. PB f25.
Immunochemistry II-Edited by A. C. CLJELLO.IBRO Handbook methods in the Neurosciences, Vol. 14. 456~~. 1993. John Wiley, Chichester, U.K. f45.
Over the 40 years since the invention of gas liquid chromatography by Martin and James, there have been considerable developments in the techniques of lipid analysis. It is very easy to get contamination or to end up with a nasty sticky mess. This book gives practical details how to avoid the difficulties and deals with: the chemical nature of lipids; extraction of lipids and derivative formation; thin layer chromatography; gas chromatography; high performance liquid chromatography; radio tracers in lipid analysis; high resolution ‘H and ‘jC NMR; mass spectroscopy of lipids.
The first edition was published in 1983 and the present volume is updated and expanded. The methods deal with: synthetic peptides for the production of antibodies; testing antibody specificity; preparation of monoclonal antibodies; avidin-biotin; immuno gold staining; EM silver intensified colloidal gold; in situ hybridization; retrograde neuronal tracing; anterograde PHA-L tracing; golgi impregnation; ultrastructural immunocytochemistry; confocal microscopy applications; second messenger molecules-formaldehyde fixed cGMP. It gives the basic theory background for each
1981
Pergamon Press Ltd.
BOOK REVIEWS
Gene Transcription; A Practical Approach-Edited by B. D. HAME~ and S. J. HIGGINS. 364~~. 1993. IRL/Oxford University Press, Oxford. HB $60, PB $42.
Preparative Centrifugatioo; by D. RICKW~~D. 399~~. Press, Oxford. PB f25.
The initial advances were made in the study of the DNA, RNA and transcription in Escherichiu co/i and viruses. Now there have been considerable advances in the study of transcription in the eukaryote with the discovery of three distinct RNA polymerases, techniques of cloning, mutagenesis, transfection, domain mapping, promoters and enhancers. This book concentrates on RNA polymerase II. It gives practical details how to deal with: assay of gene transcription in vitro; transcription of endogenous genes in mammalian cells; in vitro transcription with nuclear extracts from differentiated tissues; trangeneic animals; eukaryotic transcription factors; protein-DNA interactions; cloning and functional analysis of heterologous eukaryotic transcription factors in yeast; transcription controls, cis-elements and rrans-factors; fractionation of nucleic acids by gel electrophoresis.
The development in instrumentation and better control systems of centrifuges has allowed the more accurate and precise separation of specific identified fractions of the cell. The days are almost gone when you had to have the powerful centrifuge placed in a shed outside some way away, in case the rotor exploded and danced through the walls like a mis-guided missile. This book gives the theory and practical details how to obtain the best conditions for sub cellular separations. It deals with: theoretical aspects of practical centrifugation (differential pelleting, gradient separations; isopychnic; self forming gradients); centrifuges and rotors; conditions for density gradient separations; characterization of macromolecules and their interactions; measurement of sedimentation coefficients; isolation and characterization of membrane and cell organelles; separation of mammalian cells; separation in zonal rotors; specification of ultracentrifuge rotors; marker enzymes and chemical assays of subcellular fractions.
Protein Engineering; A Practical Approach-Edited by A. R. REEX, M. J. E. STERNBERGand R. WETZEL. 397~~. 1992. IRL/Oxford University Press, Oxford. PB E25.
A Practical Approach--Edited 1992. IRL/Oxford University
Gastrointestinal Regulatory PeptidesEdited by D. R. BROWN. Handbook of Experimental Pharmacology, Vol. 106. 446~~. 1993. Springer Verlag, Berlin. DM 480.
The problem is to produce a specific purified protein in significant quantities. This book provides some practical details that can help in this task. The chapters deal with: structural analysis of proteins, (a) protein crystallography, (b) NMR; molecular sequence data bases and their uses; design of protein structures; from protein sequence to structure; theoretical methods in protein engineering; protein stability, (a) reversible unfolding, kinetic and thermodynamic analysis, (b) enhanced folding, stabilization suppression of aggregation in oitro and in uivo; protein turnover-possible manipulations; chemical approaches to protein engineering; antibody combining sites; display of antibodies and proteins on the surface of bacteriophage fd; expression of proteins in prokaryotes; expression of proteins in yeast; genetic engineering of tumor necrosis factor; production of alpha I-antitrypsin-a case study.
The GI regulatory peptides (GIRP) have received increased interest since it was realized that many GIRP have regulatory roles in the CNS and other body systems. This book deals with: the localization and co-localization of GIRP; regulation of GIRP gene expression; post translation processing of GIRP, regulation of peptide secretion of endocrine cells; peptide receptors and signal transduction; proteolytic inactivation of GIRP; peptidergic regulation of acid secretion; peptides and enteric neural activity; GIRP regulation of smooth muscle contractility; regulation of intestinal electrolyte transport; regulation of GI blood flow; regulation of cell proliferation through multiple signalling pathways; mucosal immune function; pathophysiology of GIRP; GIRP as therapeutic agents. The reviews are of a high standard, as to be expected of this series.
Lipid Analysis; A Practical ApproacbEdited by R. J. HAMILTON and S. HAMILTON. 310~~. 1992. IRL/Oxford University Press, Oxford. PB f25.
Immunochemistry II-Edited by A. C. CLJELLO.IBRO Handbook methods in the Neurosciences, Vol. 14. 456~~. 1993. John Wiley, Chichester, U.K. f45.
Over the 40 years since the invention of gas liquid chromatography by Martin and James, there have been considerable developments in the techniques of lipid analysis. It is very easy to get contamination or to end up with a nasty sticky mess. This book gives practical details how to avoid the difficulties and deals with: the chemical nature of lipids; extraction of lipids and derivative formation; thin layer chromatography; gas chromatography; high performance liquid chromatography; radio tracers in lipid analysis; high resolution ‘H and ‘jC NMR; mass spectroscopy of lipids.
The first edition was published in 1983 and the present volume is updated and expanded. The methods deal with: synthetic peptides for the production of antibodies; testing antibody specificity; preparation of monoclonal antibodies; avidin-biotin; immuno gold staining; EM silver intensified colloidal gold; in situ hybridization; retrograde neuronal tracing; anterograde PHA-L tracing; golgi impregnation; ultrastructural immunocytochemistry; confocal microscopy applications; second messenger molecules-formaldehyde fixed cGMP. It gives the basic theory background for each
1981