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Presence and distribution of nervous system — associated mast cells which may modulate experimental autoimmune encephalomyelitis
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MEASLES VIRUS INFECTION CAUSES EXPRESSION IN RAT BRAIN
OF CLASS
I AND CLASS II ANIIGENS
T. 01sson 2, J. blaehlen I, A. L6ve 1, L. Klareskog 4, E. Norrby 3 and K. Kristensson 1 i 2 3 Dept. Pathol. and Neurol., Hudding~ University Hospital, Dept. Virol, SBL, Karolinska Institute, Stockholm and -Dept. Med. Phys. Chem., Uppsala University, Uppsala, Sweden. An important factor for the outcome of a virus infection is the occurrence of cells expressing major histocompatibility (MHC) class I and class II antzgens sznce activation and function of T-cells are dependent on recognition of the virus antzqen in context with these molecules. It is interesting that the normal central nervous system has low levels of both class I and class II MHC antigens. We have asked to what extent these antigens can be Induced during fatal or non-fatal measles vlrus encephalitis zn the rat. In both sltuations the virus induced a marked increase in both class I and class II antigens as detected by immunostaining. The distribution of these molecules in the brain was much more widespread than the occurrence of viral antigen suggesting a soluble factor for their inductlon. In 14-day-old rats with a non-fatal infection there was a marked infzltration of T-lymphocytes of "cytotoxlc/suppressor" phenotype in the brain parenchyma, whereas T "helper" cell phenotypes mainly were located perivascularly. In brains from newborn rats with a fatal infection no or only few Iymphocytes were detected. Whether age-dependent f-cell response ts related to different of the dlsease is under investigation.
clinlcal
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PRESENCE AND DISTRIBUTION OF NERVOUS SYSTEM ASSOCIATED MAST CELLS WHICH MAY MODULATE EXPERIMENTALAUTOIMMUNE ENCEPHALOMYELITIS. Edward L. Orr, Ph.D., Dept. of Anatomy, Texas College of Osteopathic Medicine, Fort Worth, Texas, U.S.A. -
The presence and d i s t r i b u t i o n of mast c e l l s associated with the surfaces of the b r a i n , s p i n a l cord, dorsal r o o t g a n g lia (DRG's), and meninges have been evaluated in SJL mice as a prelude to i n v e s t i g a t i n g the role of these c e l l s in m e d i a t i n g or m o d u l a t i n g the development of the inflammation and edema of experimental autoimmune encephalomyelitis (EAE). To date, we have determined t h a t numerous mast c e l l s are c o n s i s t e n t l y found in association with the sinuses, blood vessels, and nerves of the cranial and spinal dura mater, and with the periosteum of the spinal canal. Numerous mast c e l l s are also found associated with the tissues and blood vessels of the velum interpositum, and along the trunks and larger branches of the cerebral a r t e r i e s . Substantial numbers of mast c e l l s are also present in the leptomeninges overlying the dorsal cerebellum, and in the leptomeninges found overlying and just caudal to the optic chiasma. F i n a l l y , large numbers of mast c e l l s are found e n c i r c l i n g the circumference of the DRG's, although the absolute number of mast c e l l s around each DRG depends on i t s size and location along the spinal canal. Thus, mast c e l l s are found p r i m a r i l y associated with blood vessels of the meninges, brain, spinal cord, and DRG's, and, thus, are i d e a l l y located to mediate the development of the inflammation and edema of EAE. Supported by an Organized Research Grant from T.C.O.M.
MEASLES VIRUS INFECTION CAUSES EXPRESSION IN RAT BRAIN
OF CLASS
I AND CLASS II ANIIGENS
T. 01sson 2, J. blaehlen I, A. L6ve 1, L. Klareskog 4, E. Norrby 3 and K. Kristensson 1 i 2 3 Dept. Pathol. and Neurol., Hudding~ University Hospital, Dept. Virol, SBL, Karolinska Institute, Stockholm and -Dept. Med. Phys. Chem., Uppsala University, Uppsala, Sweden. An important factor for the outcome of a virus infection is the occurrence of cells expressing major histocompatibility (MHC) class I and class II antzgens sznce activation and function of T-cells are dependent on recognition of the virus antzqen in context with these molecules. It is interesting that the normal central nervous system has low levels of both class I and class II MHC antigens. We have asked to what extent these antigens can be Induced during fatal or non-fatal measles vlrus encephalitis zn the rat. In both sltuations the virus induced a marked increase in both class I and class II antigens as detected by immunostaining. The distribution of these molecules in the brain was much more widespread than the occurrence of viral antigen suggesting a soluble factor for their inductlon. In 14-day-old rats with a non-fatal infection there was a marked infzltration of T-lymphocytes of "cytotoxlc/suppressor" phenotype in the brain parenchyma, whereas T "helper" cell phenotypes mainly were located perivascularly. In brains from newborn rats with a fatal infection no or only few Iymphocytes were detected. Whether age-dependent f-cell response ts related to different of the dlsease is under investigation.
clinlcal
courses
PRESENCE AND DISTRIBUTION OF NERVOUS SYSTEM ASSOCIATED MAST CELLS WHICH MAY MODULATE EXPERIMENTALAUTOIMMUNE ENCEPHALOMYELITIS. Edward L. Orr, Ph.D., Dept. of Anatomy, Texas College of Osteopathic Medicine, Fort Worth, Texas, U.S.A. -
The presence and d i s t r i b u t i o n of mast c e l l s associated with the surfaces of the b r a i n , s p i n a l cord, dorsal r o o t g a n g lia (DRG's), and meninges have been evaluated in SJL mice as a prelude to i n v e s t i g a t i n g the role of these c e l l s in m e d i a t i n g or m o d u l a t i n g the development of the inflammation and edema of experimental autoimmune encephalomyelitis (EAE). To date, we have determined t h a t numerous mast c e l l s are c o n s i s t e n t l y found in association with the sinuses, blood vessels, and nerves of the cranial and spinal dura mater, and with the periosteum of the spinal canal. Numerous mast c e l l s are also found associated with the tissues and blood vessels of the velum interpositum, and along the trunks and larger branches of the cerebral a r t e r i e s . Substantial numbers of mast c e l l s are also present in the leptomeninges overlying the dorsal cerebellum, and in the leptomeninges found overlying and just caudal to the optic chiasma. F i n a l l y , large numbers of mast c e l l s are found e n c i r c l i n g the circumference of the DRG's, although the absolute number of mast c e l l s around each DRG depends on i t s size and location along the spinal canal. Thus, mast c e l l s are found p r i m a r i l y associated with blood vessels of the meninges, brain, spinal cord, and DRG's, and, thus, are i d e a l l y located to mediate the development of the inflammation and edema of EAE. Supported by an Organized Research Grant from T.C.O.M.