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Pressure-diameter characteristics of the rat saphenous vein in vivo. Effects of using different technics to change intraluminal pressure
PRESSURE-DIAMETER CHARACTERISTICS OF THE RAT SAPHENOUS VEIN IN VIVO. EFFECTS OF USING DIFFERENT TECHNICS TO CHANGE INTRALUMINAL PRESSURE G.L. Nadasy, M. Lorant, M. Szentivanyi Jr., M. Toth. E.
PLATELET AND MYOCARDIAL MAST CELLS AS THE SOURCE OF STIMULATING NEOANGIOGENESIS FACTORS IN TMLR OF THE HEART G. K. Popov, E. S. Golovneva, M. S. Belsky
Monos Exp Res Dept and 2nd Inst Physiol, Semmelweis Univ Med, Budqwst, Hunga~ Aim of study: To test whether the observed in vivo rigidity of the rat saphenous vein depends on the dynamics of intraluminal pressure change and the technics of its the inducement. Methods: Intraluminal pressure in saphenous vein of anesthetized rats was changed by (i) graded occlusion of the thigh with an elastic thread at different rates, (ii) infusion of same . 1’ and withdrawal of blood through a side branch at different rates (iii) acute head-up and head-down tilt positions. Outer diameter of the exposed saphenous artery was measured by an in vivo Results: Independently of the method microangiograph. applied and of the rate of pressure change the vessels were showing substantial rigidity with the exception of lowest pressures (
Chelyabinsk State Institute of Laser Surgery, Chelyabinsk, Russia
smooth
muscle
contractlon-relaxation,
role can be attributed to distensibility. and CO I94B)
only
The aim of our study was to investigate the dynamic of morfofunctional changes of platelets and myocardial mast cells at the place of TMLR. Methods: TMLR model is reproduced in 90 Wistar rats using YAG-Nd laser (P=lOW, t=l-2 sec., CW). l-2 laser channels have been created. Platelets response was evaluated takiig into consideration their ultrastructure with electron microscopy. The mast cells were revealed by toluidin blue staining in serial myocardial slides. Results: ‘I’MLR proved to cause destruction and aggregation of the platelets in the laser channel and surrounding areas. The evident phenomenon of the mast cells degranulation in above mentioned heart areas was noted during the period from 1 to 20 days since the experiment. The degmnulation phenomenon of myocardial mast cells was preceded to the new microvessels genesis near laser channels. Conclusion: Degranulation platelets and myocardial mast cells seem to be the source of growth factors inducing neoangiogenesis.
a moderate
(OTKA TOOl77-95
FUNCTIONAL MORPHOLOGY OF BLOOD CAPILLARIES OF THE DOGS PAROTID GLAND AFTER DUCT LIGATION G. Salms, A. Dalmane, A. Skagers Department of Maxillofacial Surgery and Department of Histology, Riga Paul Stradina University, Latvia
p-HYDROXYPHENYLACETALDEHYDE (pHA), A PRODUCT OF L-TYROSINE OXIDATION BY THE MYELOPEROXIDASE SYSTEM OF PHAGOCYTES, INHIBITS ANGIOGENESIS S. Oh-ishi, H. Miyazaki, J. W. Heinecke, C. Fujii, T. Kizaki, T. Ookawara, S. Haga, C. Nakao, Y. Sato, and H. Ohno National Defense Medical College, University of Tsukuba, Osaka Prefectural Junior College of Health Science, Nagoya University, Japan, and Washington Universiry School of Medicine, USA
Parenchymal functional morphology of salivary glands are dependent from alteration in blood circulation. Embranchement of salivary glands blood capillaries are complicated because two capillary nets are organised. First are made around striated ducts but second is more narrowed around acinuses. Due to different pathologic situations as sialolithiasis first changes affect secondary capillary netting. It was observed after ligation of dogs salivary ducts. Morphology of blood capillaries are forming joint functional unit with connective tissue elements. Endothelium of salivary glands are fenestrated. Fingertip microvilli are on close contact to cell surface. There are two kind of endotheliocytes pale and dark can be divided by cytoplasmatic electron optical density. Lamina densa are fixed with thin anchoraging fibrils of collagen. Around blood capillaries plasmocytes connective tissue fibres and unmyelinated cable type nerve fibers has observed. Twenty days after ligation of salivary glands ducts complex changes in parenchyma are seen. Primary impairment is in blood capillary wall. Dark endotheliocytes are increase in quantity on blood capillary wall. Fibrillar structure of nucleus has lost but granular part still is present. Margination of heterochromatine are suggested about picnotic changes of nucleus. Cavity of dilatated endoplasmatic reticulum contain microgranular material. Bound ribosomas has disappeared. Basal plate are thickened in contact to apoptotic endotheliocytes. Plasmocytes and nerve tibres has dystrophic changes. We found that primary capillary reticulum around striated ducts had less changes in structure than reticulum surrounded by acinuses. We suppose that striated ducts were less injured matched to the acinuses.
Angiogenesis, the formation of new capillaries, is of central importance of physiological and pathophysiological processes, ranging from embryogenesis to wound healing, tumor growth and metastasis, and collateral circulation in atherosclerotic obstructive diseases. During exercise, capillary vessels in skeletal muscles are the major source of oxygen and nutrients. Exercise is also known to promote the infiltration of inflammatory cells like neutrophils into injured skeletal muscles. The aim of the present study was to investigate the role of oxidative products generated by phagocytes on capillary growth, using an in vitro angiogenesis system. The model system consisted of bovine capillary endothelial cells (BCEC) and First, we confirmed that rat smooth muscle cells. activated phagocytes converted L-tyrosine to the reactive aldehyde, pHA, through the myeloperoxidase (MPO) /peroxide/chloride system. Next, we examined the effect df pHA on angiogenesis. pHA dramatically inhibited the formation of caoillarv network. Moreover, pHA exerted cytotoxic effect’s on 6CEC. These observa
172
PLATELET AND MYOCARDIAL MAST CELLS AS THE SOURCE OF STIMULATING NEOANGIOGENESIS FACTORS IN TMLR OF THE HEART G. K. Popov, E. S. Golovneva, M. S. Belsky
Monos Exp Res Dept and 2nd Inst Physiol, Semmelweis Univ Med, Budqwst, Hunga~ Aim of study: To test whether the observed in vivo rigidity of the rat saphenous vein depends on the dynamics of intraluminal pressure change and the technics of its the inducement. Methods: Intraluminal pressure in saphenous vein of anesthetized rats was changed by (i) graded occlusion of the thigh with an elastic thread at different rates, (ii) infusion of same . 1’ and withdrawal of blood through a side branch at different rates (iii) acute head-up and head-down tilt positions. Outer diameter of the exposed saphenous artery was measured by an in vivo Results: Independently of the method microangiograph. applied and of the rate of pressure change the vessels were showing substantial rigidity with the exception of lowest pressures (
Chelyabinsk State Institute of Laser Surgery, Chelyabinsk, Russia
smooth
muscle
contractlon-relaxation,
role can be attributed to distensibility. and CO I94B)
only
The aim of our study was to investigate the dynamic of morfofunctional changes of platelets and myocardial mast cells at the place of TMLR. Methods: TMLR model is reproduced in 90 Wistar rats using YAG-Nd laser (P=lOW, t=l-2 sec., CW). l-2 laser channels have been created. Platelets response was evaluated takiig into consideration their ultrastructure with electron microscopy. The mast cells were revealed by toluidin blue staining in serial myocardial slides. Results: ‘I’MLR proved to cause destruction and aggregation of the platelets in the laser channel and surrounding areas. The evident phenomenon of the mast cells degranulation in above mentioned heart areas was noted during the period from 1 to 20 days since the experiment. The degmnulation phenomenon of myocardial mast cells was preceded to the new microvessels genesis near laser channels. Conclusion: Degranulation platelets and myocardial mast cells seem to be the source of growth factors inducing neoangiogenesis.
a moderate
(OTKA TOOl77-95
FUNCTIONAL MORPHOLOGY OF BLOOD CAPILLARIES OF THE DOGS PAROTID GLAND AFTER DUCT LIGATION G. Salms, A. Dalmane, A. Skagers Department of Maxillofacial Surgery and Department of Histology, Riga Paul Stradina University, Latvia
p-HYDROXYPHENYLACETALDEHYDE (pHA), A PRODUCT OF L-TYROSINE OXIDATION BY THE MYELOPEROXIDASE SYSTEM OF PHAGOCYTES, INHIBITS ANGIOGENESIS S. Oh-ishi, H. Miyazaki, J. W. Heinecke, C. Fujii, T. Kizaki, T. Ookawara, S. Haga, C. Nakao, Y. Sato, and H. Ohno National Defense Medical College, University of Tsukuba, Osaka Prefectural Junior College of Health Science, Nagoya University, Japan, and Washington Universiry School of Medicine, USA
Parenchymal functional morphology of salivary glands are dependent from alteration in blood circulation. Embranchement of salivary glands blood capillaries are complicated because two capillary nets are organised. First are made around striated ducts but second is more narrowed around acinuses. Due to different pathologic situations as sialolithiasis first changes affect secondary capillary netting. It was observed after ligation of dogs salivary ducts. Morphology of blood capillaries are forming joint functional unit with connective tissue elements. Endothelium of salivary glands are fenestrated. Fingertip microvilli are on close contact to cell surface. There are two kind of endotheliocytes pale and dark can be divided by cytoplasmatic electron optical density. Lamina densa are fixed with thin anchoraging fibrils of collagen. Around blood capillaries plasmocytes connective tissue fibres and unmyelinated cable type nerve fibers has observed. Twenty days after ligation of salivary glands ducts complex changes in parenchyma are seen. Primary impairment is in blood capillary wall. Dark endotheliocytes are increase in quantity on blood capillary wall. Fibrillar structure of nucleus has lost but granular part still is present. Margination of heterochromatine are suggested about picnotic changes of nucleus. Cavity of dilatated endoplasmatic reticulum contain microgranular material. Bound ribosomas has disappeared. Basal plate are thickened in contact to apoptotic endotheliocytes. Plasmocytes and nerve tibres has dystrophic changes. We found that primary capillary reticulum around striated ducts had less changes in structure than reticulum surrounded by acinuses. We suppose that striated ducts were less injured matched to the acinuses.
Angiogenesis, the formation of new capillaries, is of central importance of physiological and pathophysiological processes, ranging from embryogenesis to wound healing, tumor growth and metastasis, and collateral circulation in atherosclerotic obstructive diseases. During exercise, capillary vessels in skeletal muscles are the major source of oxygen and nutrients. Exercise is also known to promote the infiltration of inflammatory cells like neutrophils into injured skeletal muscles. The aim of the present study was to investigate the role of oxidative products generated by phagocytes on capillary growth, using an in vitro angiogenesis system. The model system consisted of bovine capillary endothelial cells (BCEC) and First, we confirmed that rat smooth muscle cells. activated phagocytes converted L-tyrosine to the reactive aldehyde, pHA, through the myeloperoxidase (MPO) /peroxide/chloride system. Next, we examined the effect df pHA on angiogenesis. pHA dramatically inhibited the formation of caoillarv network. Moreover, pHA exerted cytotoxic effect’s on 6CEC. These observa
172