- Email: [email protected]
Process for the preparation of human leukocyte and human gamma interferon
New Patents
A probe and method for treating samples in a well tray. The probe is specially configured to engage the well, and when so engaged, to provide an electrode structure within the well. The probe comprises an outer electrode in the form of a metal ring having a diameter just slightly less than the inner diameter of the well, a center electrode in the form of a metal rod, and a nonconductive (typically clear plastic) cover that extends over the well and holds the electrodes coaxial with respect to each other and with respect to the well. A metal pin is rigidly connected to the ring and extends parallel to the ring axis. The rod and pin pass through the cover so that their upper ends are accessible for electrical connections. Electrical connections are made to the portions of the rod and the pin located above the cover. As a safety measure, the electrical connections are preferably hidden under a cap that threadably engages the top portion of the rod and overlies the cover.
4695548 GEL INSERTS USEFUL IN ELECTROPHORESIS Charles R Cantor, David C Schwartz assigned to The Trustees of Columbia University in the City of New York Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.
4696897 PROCESS FOR PREPARING 2-KETO-L-GULONIC ACID Takayasu Sonoyama, Shigeo Yagi, Bunji Kageyama, Osaka, Japan assigned to Shionogi & Co Ltd 2-Keto-L-gulonic acid is prepared directly from D-glucose by microbial conversion utilizing mixed culturing on a medium containing Dglucose, employing two kinds of microorganisms; the first, a 2,5-diketo-D-gluconic acid producing microorganism which belongs to
”
genus Erwinia and the second, a 2-keto-Lgulonic acid producing microorganism which belongs to genus Brevibacterium or Corynebacterium. The incubation of the microorganisms in a medium containing D-glucose is used in the disclosed process. By-production of 2-keto-Dgluconic acid, the undesired isomer of the intended product is effectively prevented by employing mixed culturing because of the coexistence of both microorganisms in the medium during at least part of the entire cultivation. Namely, 2-keto-D-gluconic acid produced by the second microorganism is utilized by the first microorganism to produce 2,5-diketo-Dgluconic acid which is subsequently converted into 2-keto-L-gulonic acid by the second microorganism.
4696898 VECTOR ENCODING HEPATITIS B SURFACE ANTIGEN Renee A Fitts, Nanc Hsiung, Dean H Hamer assigned to Integrated Genetics Inc Recombinant DNA vector including (1) a promoter for a eukaryotic metalliothionein gene ligated to a gene sequence encoding hepatitis B surface antigen, and (2) at least the 69% transforming region of the bovine papilloma genome, expression of the hepatitis B surface antigen encoding gene sequence being under the control of the metallothionein promoter.
PROCESS FOR THE PREPARATION OF HUMAN LEUKOCYTE AND HUMAN GAMMA INTERFERON Miklos Toth, Valeria Endresz, Ilona Beladi, Sandor Toth, Szeged, Hungary assigned to EGYT Gyogyszervegyeszeti Gyar The invention relates to a process for the preparation of E60 - and +65 -interferon by isolating the buffy coat fraction of blood, removing the erythrocytes, suspending the leukocytes in a suitable nutrient medium and treating with an +60 -interferon inducer and with a + 65 -interferon inducer, which comprises producing + 60 -interferon and + 65 -interferon one after the other in the same leukocyte culture by pre-treating the suspension obtained after the separation of the leukocyte/huffy coat/fraction
New Patents
M
of blood, removal of the erythrocytes and suspending the leukocytes in a suitable nutrient medium with +O - or +62 -interferon, contacting with an +60 -interferon inducer, separating the liquid containing the +60 interferon from the cells, if desired recovering the + 0 -interferon, washing and suspending the cells in a suitable nutrient medium, treating with a mitogenic agent, separating the liquid comprising +60 -interferon from the cells and if desired recovering + 60 -interferon and if desired making + 60 -interferon free of + 60 -interferon. + RE + PA The advantage of the process is that +60 - and $65 -interferon are produced from the same leukocyte culture in two steps. This makes the process more economical and enables an effective utilization of the limitedly available Ieukocytes. + RE
4696937 THIOSULFONATE DERIVAT~ES FOR IN~BITING MALFORMATION OR DESTRUCTION OF RED BLOOD CORPUSCULES Bernard Sebille, Yves Beuzard, Hem-i Demame, Clamart, France assigned to Sanofi; Institut National de la Sante et de la Recherche Medicale (Inser The invention relates to novel thiosulfonate derivatives of general formula: See Patent for Tabular Presentation PS in which R represents a 1H-imidazol-Zyl, quinolin-Zyl, 5-amino-1,3,4thiadizol-Zyl or 4, S-dihydro-thiazol-2-yl radical and RI represents a lower alkyl, (hydroxy)lower alkyl, phenyl, (lower alkyl) phenyl, (hydroxy lower alkyl) phenyl, (lower alkanoyl) phenyl or quinolin-&yl radical. The thiosulfonate derivatives in question exert an inhibitory action with respect to the malformation or destruction of red blood corpuscules due to a genetic modification of haemoglobin or to parasites and can, therefore, be used in the treatment of drepanocytosis, malaria and babebiosis.
The adsorbent consists of a solid phase, completely or partially penetrated by or surfacecoated with a hydrophilic molecular polymer netting to which has been bound substituents or cross bridges having chain sequences of the structure See Patent for Tabular Presentation PS where X is ether oxygen, a thioether sulfur atom or a nitrogen atom and Y is a substituted or unsubstituted alkyl, aryl or heteroaromatic group. The solid phase consists of particles with a diameter of less than 1 mm and the molecular polymer netting consisting of a cross-linked polyhydroxy polymer such as a polysaccharide, preferably a polygalactane such as agar or agarose or a cross-linked ~lyamide, e.g. polyacryl amide. Y can be hydroxy alkyl or mercapto alkyl, or phenyl alkyl or phenyl substituted with halogen or nitro groups. The adsorbent is prepared by first converting in a known manner a hydrophilic polymer containing OH and/or CONH2 groups to a vinylsulfone substituted polymer for subsequent contact in alkali solution, with or without organic solvent, with an organic thiol compound. The adsorbent can be used for the fractionation of biopolymers such as nucleic acids, nucleotides, proteins and potypeptides, and has been found particularly well suited for the fractionation of serum proteins.
4697002 CALCITONIN GENE RELATED PEPTIDE ANALOGS WITH AMINO ACID SUBDSTITUENTS AT THE PENULTIMATE POSITION 36 Tomas Kempe New calcitonin gene related peptide analogs (CGRP) are disclosed which have biological activity of the same type as known calcitonin gene related peptides and which have an amino acid substituent at position 36 or the penultimate position instead of the naturally occurring amino acid&l 3 Ala. f RI?
4697242 4696980 SULFONE ACTIVATED THIOETHXR ADSORBENTS FOR THE SEPARATION OF PROTEINS AND THE LIKE Jerker Porath, S 18136 Liding Sweded
ADAPTIVE COMPUTING SYSTEM CAPABLE OF LEARNING AND DISCOVERY John H Holland, Arthur W Burks An electronic computing system capable of learning and discovery employing a language com-
A probe and method for treating samples in a well tray. The probe is specially configured to engage the well, and when so engaged, to provide an electrode structure within the well. The probe comprises an outer electrode in the form of a metal ring having a diameter just slightly less than the inner diameter of the well, a center electrode in the form of a metal rod, and a nonconductive (typically clear plastic) cover that extends over the well and holds the electrodes coaxial with respect to each other and with respect to the well. A metal pin is rigidly connected to the ring and extends parallel to the ring axis. The rod and pin pass through the cover so that their upper ends are accessible for electrical connections. Electrical connections are made to the portions of the rod and the pin located above the cover. As a safety measure, the electrical connections are preferably hidden under a cap that threadably engages the top portion of the rod and overlies the cover.
4695548 GEL INSERTS USEFUL IN ELECTROPHORESIS Charles R Cantor, David C Schwartz assigned to The Trustees of Columbia University in the City of New York Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.
4696897 PROCESS FOR PREPARING 2-KETO-L-GULONIC ACID Takayasu Sonoyama, Shigeo Yagi, Bunji Kageyama, Osaka, Japan assigned to Shionogi & Co Ltd 2-Keto-L-gulonic acid is prepared directly from D-glucose by microbial conversion utilizing mixed culturing on a medium containing Dglucose, employing two kinds of microorganisms; the first, a 2,5-diketo-D-gluconic acid producing microorganism which belongs to
”
genus Erwinia and the second, a 2-keto-Lgulonic acid producing microorganism which belongs to genus Brevibacterium or Corynebacterium. The incubation of the microorganisms in a medium containing D-glucose is used in the disclosed process. By-production of 2-keto-Dgluconic acid, the undesired isomer of the intended product is effectively prevented by employing mixed culturing because of the coexistence of both microorganisms in the medium during at least part of the entire cultivation. Namely, 2-keto-D-gluconic acid produced by the second microorganism is utilized by the first microorganism to produce 2,5-diketo-Dgluconic acid which is subsequently converted into 2-keto-L-gulonic acid by the second microorganism.
4696898 VECTOR ENCODING HEPATITIS B SURFACE ANTIGEN Renee A Fitts, Nanc Hsiung, Dean H Hamer assigned to Integrated Genetics Inc Recombinant DNA vector including (1) a promoter for a eukaryotic metalliothionein gene ligated to a gene sequence encoding hepatitis B surface antigen, and (2) at least the 69% transforming region of the bovine papilloma genome, expression of the hepatitis B surface antigen encoding gene sequence being under the control of the metallothionein promoter.
PROCESS FOR THE PREPARATION OF HUMAN LEUKOCYTE AND HUMAN GAMMA INTERFERON Miklos Toth, Valeria Endresz, Ilona Beladi, Sandor Toth, Szeged, Hungary assigned to EGYT Gyogyszervegyeszeti Gyar The invention relates to a process for the preparation of E60 - and +65 -interferon by isolating the buffy coat fraction of blood, removing the erythrocytes, suspending the leukocytes in a suitable nutrient medium and treating with an +60 -interferon inducer and with a + 65 -interferon inducer, which comprises producing + 60 -interferon and + 65 -interferon one after the other in the same leukocyte culture by pre-treating the suspension obtained after the separation of the leukocyte/huffy coat/fraction
New Patents
M
of blood, removal of the erythrocytes and suspending the leukocytes in a suitable nutrient medium with +O - or +62 -interferon, contacting with an +60 -interferon inducer, separating the liquid containing the +60 interferon from the cells, if desired recovering the + 0 -interferon, washing and suspending the cells in a suitable nutrient medium, treating with a mitogenic agent, separating the liquid comprising +60 -interferon from the cells and if desired recovering + 60 -interferon and if desired making + 60 -interferon free of + 60 -interferon. + RE + PA The advantage of the process is that +60 - and $65 -interferon are produced from the same leukocyte culture in two steps. This makes the process more economical and enables an effective utilization of the limitedly available Ieukocytes. + RE
4696937 THIOSULFONATE DERIVAT~ES FOR IN~BITING MALFORMATION OR DESTRUCTION OF RED BLOOD CORPUSCULES Bernard Sebille, Yves Beuzard, Hem-i Demame, Clamart, France assigned to Sanofi; Institut National de la Sante et de la Recherche Medicale (Inser The invention relates to novel thiosulfonate derivatives of general formula: See Patent for Tabular Presentation PS in which R represents a 1H-imidazol-Zyl, quinolin-Zyl, 5-amino-1,3,4thiadizol-Zyl or 4, S-dihydro-thiazol-2-yl radical and RI represents a lower alkyl, (hydroxy)lower alkyl, phenyl, (lower alkyl) phenyl, (hydroxy lower alkyl) phenyl, (lower alkanoyl) phenyl or quinolin-&yl radical. The thiosulfonate derivatives in question exert an inhibitory action with respect to the malformation or destruction of red blood corpuscules due to a genetic modification of haemoglobin or to parasites and can, therefore, be used in the treatment of drepanocytosis, malaria and babebiosis.
The adsorbent consists of a solid phase, completely or partially penetrated by or surfacecoated with a hydrophilic molecular polymer netting to which has been bound substituents or cross bridges having chain sequences of the structure See Patent for Tabular Presentation PS where X is ether oxygen, a thioether sulfur atom or a nitrogen atom and Y is a substituted or unsubstituted alkyl, aryl or heteroaromatic group. The solid phase consists of particles with a diameter of less than 1 mm and the molecular polymer netting consisting of a cross-linked polyhydroxy polymer such as a polysaccharide, preferably a polygalactane such as agar or agarose or a cross-linked ~lyamide, e.g. polyacryl amide. Y can be hydroxy alkyl or mercapto alkyl, or phenyl alkyl or phenyl substituted with halogen or nitro groups. The adsorbent is prepared by first converting in a known manner a hydrophilic polymer containing OH and/or CONH2 groups to a vinylsulfone substituted polymer for subsequent contact in alkali solution, with or without organic solvent, with an organic thiol compound. The adsorbent can be used for the fractionation of biopolymers such as nucleic acids, nucleotides, proteins and potypeptides, and has been found particularly well suited for the fractionation of serum proteins.
4697002 CALCITONIN GENE RELATED PEPTIDE ANALOGS WITH AMINO ACID SUBDSTITUENTS AT THE PENULTIMATE POSITION 36 Tomas Kempe New calcitonin gene related peptide analogs (CGRP) are disclosed which have biological activity of the same type as known calcitonin gene related peptides and which have an amino acid substituent at position 36 or the penultimate position instead of the naturally occurring amino acid&l 3 Ala. f RI?
4697242 4696980 SULFONE ACTIVATED THIOETHXR ADSORBENTS FOR THE SEPARATION OF PROTEINS AND THE LIKE Jerker Porath, S 18136 Liding Sweded
ADAPTIVE COMPUTING SYSTEM CAPABLE OF LEARNING AND DISCOVERY John H Holland, Arthur W Burks An electronic computing system capable of learning and discovery employing a language com-