- Email: [email protected]
Production of extracellular alkaline protease by new halotolerant alkaliphilic Bacillus sp. NPST-AK15 isolated from hyper saline soda lakes
Abstracts / Journal of Biotechnology 208 (2015) S5–S120
impacted, it has a molecular attraction that resists a tendency to flow and the resistance to penetration is direct proportional to the projectile’s velocity. For a tissue which is primarily like water, the resistance to penetration is proportional to the velocity squared. The brain is included in this group of materials. This paper shows the experimental results of impact tests using different weapon systems and ballistic gelatin. Lethal and non-lethal ammunition were shot. Also, a laboratory test for an arrow ammunition was performed in order to evaluate the impact with human tissue. The gelatin is widely used in these experiments because it is agreed to similarly respond to living tissue, without bones and muscles. The 20% (by mass) ballistic gelatin used in tests was made according to a NATO Allied Engineering Publication, Skin penetration assessment of non-lethal projectiles. Further, computer simulations of impact in gelatin were developed, both for reproducing the real tests and to analyze new loading cases. They give strong perspectives about tissue behavior and protective requirements. http://dx.doi.org/10.1016/j.jbiotec.2015.06.026
Oral Presentation Texts Using molecular tools to investigate the epidemiology of tick-borne diseases in Romania Mariana Ionita 1,∗ , Cornelia Silaghi 2 , Ioan Liviu Mitrea 1 , Kurt Pfister 2 1 Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agronomic Science and Veterinary Medicine, Bucharest, Romania 2 Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München, Germany
E-mail address: [email protected] (M. Ionita). In recent years, there has been increasing concern about the global spread of parasitic arthropods and the pathogens they transmit. Ticks are recognized among the most important vectors of diseases affecting both humans and animals. Ticks carry a wide range of pathogens (bacteria, viruses, protozoan) with an impact on animal and human health; some of these are recognized as important (re)emerging pathogens. The knowledge of pathogens transmitted by ticks in a given area is useful for assessing the risk of infection in humans and animals. One of the first steps for tick-borne disease risk assessment is the detection of the causing pathogens in their vectors. PCR amplification of pathogen DNA using species-specific primers is the standard for pathogen detection in ticks. In Romania, there is a very diverse ixodid tick fauna. Therefore, to assess the implication of ticks in the epidemiology of tick-borne diseases in Romania, a total of 619 ticks, including questing ticks and ticks collected from natural infected domestic animals (cattle, sheep, horses, dogs), were molecularly screened for tick-borne pathogens (TBP) using conventional PCR followed by sequencing or RealTime PCR. Tick species analyzed encompassed six species from different genera (Ixodes ricinus, Dermacentor marginatus, Dermacentor reticulatus, Rhipicephalus sanguineus sensu lato, Rhipicephalus bursa, Hyalomma marginatum). The results showed a wide distribution of very diverse bacterial (Rickettsia spp., Anaplasma phagocytophilum, Borrelia afzelii, Ehrlichia canis) and protozoan (Babesia spp., Theileria spp.) pathogens. These findings emphasize real risks for animal and human health in Romania and expand the known distribution of TBP in Europe. http://dx.doi.org/10.1016/j.jbiotec.2015.06.027
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Genetic parameters assessment in a Leghorn hen line Iuliana Neagu ∗ , Magda Gonciarov, Carmen Daniela Petcu, Margarita Oana Ghimpeteanu Department of Animal Production and Public Health, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania E-mail address: iuliana [email protected] (I. Neagu). The correct management of the breeding programs supposes the assessment of the genetic parameters in every generation of that population. The aim of the research consisted in the assessment of the genetic parameters of a Leghorn line by Restricted Maximum Likelihood method which assures positive estimates. There were sampled data for the 2014 generation regarding the number of eggs, egg weight at 28 weeks, egg weight at 34 weeks, body weight and age of the first egg. For variance-covariance analyze a linear mixed model was used (yij = + Ai + Cij ). The heritability was calculated as a ratio between the additive genetic variance and the total phenotypic variance. The results considered that the egg weight at 28 weeks (h2 = 0.216), the egg weight at 34 weeks (h2 = 0.214) and the body weight (h2 = 0.226), being considered intermediary transmitting characters. The number of eggs recorded a value of h2 = 0.036 and the age of the first egg h2 = 0.054, these characters being low heritable. The phenotypic correlation between the egg number and the age of first egg (rF = −0.423), is a very strict negative value and reveals the fact that hens which started laying eggs early are also good egg producers. The genotypic correlation between the egg number and the age of first egg (rG = 0.552) was very strict positive which shows that very early hens are not good egg producers, so a correlated for these characters selection could not be applied.
http://dx.doi.org/10.1016/j.jbiotec.2015.06.028 Production of extracellular alkaline protease by new halotolerant alkaliphilic Bacillus sp. NPST-AK15 isolated from hyper saline soda lakes Abdelnasser Salah Shebl Ibrahim ∗ , Ali A. Al Salamah, Yahya B. El Badawil, Mohamed A. El Tayeb Department of Botany and Microbiology, College of Science, King Saud University, Riyadh Saudi Arabia E-mail address: [email protected] (A.S.S. Ibrahim). Alkaline proteases are among the most important classes of industrial hydrolytic enzymes. The industrial demand for alkaline proteases with favorable properties continues to enhance the search for new enzymes. A new potent alkaline protease producing halotolerant alkaliphilic bacterium termed NPST-AK15, was isolated from hypersaline soda lakes, which affiliated to Bacillus sp strain NPST-AK15 based on 16S rRNA gene analysis (accession no. KP295749). Maximal protease production was obtained using yeast extract and fructose as nitrogen and carbon source, respectively. Strain NPST-AK15 can grow over a wide range of NaCl concentrations (0–20%), showing maximal growth and enzyme production at 0–5%, indicated the halotolerant nature of this bacterium. Ba and Ca ions enhanced enzyme production by about 1.6 and 1.3-fold respectively. The optimum temperature and pH for both enzyme production and cell growth was seen at 40 ◦ C and pH 11, respectively. Alkaline protease secretion was coherent with the growth
S14
Abstracts / Journal of Biotechnology 208 (2015) S5–S120
pattern, started at beginning of the exponential phase and reached maximal in mid stationary phase (36 h). Briefly, optimization of various fermentation parameters resulted in an increase of enzyme yield by 22.8-fold. NPST-AK15 and its extracellular alkaline protease with salt tolerance signify their potential applications in detergent formulations and other industries. http://dx.doi.org/10.1016/j.jbiotec.2015.06.029 Comparison of aroma production from castor oil by Yarrowia lipolytica in airlift and STR bioreactors Isabel Belo 1,∗ , Adelaide Braga 1 , Daniela Mesquita 1 , Luis Amaral 2 , Eugénio Ferreira 1 1
CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal 2 Instituto Politécnico de Coimbra, ISEC, DEQB, Coimbra, Portugal E-mail address: [email protected] (I. Belo). In aerobic cultures using hydrophobic substrates, such as the use of castor oil (CO) for ␥-decalactone production by Yarrowia lipolytica, the selection of bioreactor type is particularly important. Although stirred tanks (STR) are the most common industrial bioreactors used for aerobic fermentations, agitation conditions tend to cause some shearing stress to the cells. This can be prevented using aeration as driving force to promote agitation that is the principle of airlift reactors. This work analyses the influence of agitation (mechanical or pneumatic) and oxygen mass transfer coefficient (kLa) on ␥decalactone production by Y. lipolytica in a biphasic culture medium with CO as substrate. Y. lipolytica has the ability to perform the biotransformation of ricinoleic acid (the major fatty acid of CO), into ␥-decalactone through peroxisomal -oxidation. For both bioreactors ␥-decalactone productivity increased with kLa, in spite of the lower ␥-decalactone maximum concentration. On the other hand, higher oxygen transfer rate favored the accumulation of other lactone, such as 3-hydroxy-␥-decalactone. Higher values of ␥-decalactone concentration were obtained in the airlift (up to 3 g/L) compared to STR (up to 1.5 g/L). Also, the impact of agitation type in cell morphology was investigated using image analysis. Significant differences were observed between cellular populations of both bioreactors; in STR an increase in loose cells and quite irregular structures was observed, indicating that pneumatic agitation has less impact in cells morphology than mechanical agitation. http://dx.doi.org/10.1016/j.jbiotec.2015.06.030 A web-based application to inform consumers about the products based on corn and soybeans sold in Romania Domokos Alice Dorottya University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur 3-5, 400372, Cluj-Napoca, Romania
of their agricultural ingredients are organic. The use of genetically modified organisms (GMOs) and products derived from GMOs is still prohibited in the organic production. The products containing GMOs cannot be labeled as organic unless the ingredients containing GMOs have reached unintentionally in the product and if the proportion of GMOs in the ingredient is below 0.9%. The purpose of this paper is to present an interdisciplinary research by applying modern methods of computer science and molecular biology to inform the consumers about the products sold in Romania. To implement this, we creating a graphic interface, written in a component-based programming language PHP, and the information were stored in MySQL databases and it can be extracted to inform a consumer about the stored products. The database was populated with products based on corn and soybeans containing or not containing genetically modified organisms. The database also contained legislation on the marketing of these products. Products entered in the database have been tested in the laboratory “CERT-GMO” of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. The result of this process was a useful database, which contain information about products based on soy and maize sold in Romania. http://dx.doi.org/10.1016/j.jbiotec.2015.06.031 Analysis of the dynamics of the defensin from germinated lentil seeds Marian Butu 1,∗ , Steliana Rodino 2 , Marius Mihai Micu 3 , Petruta Petrache 3 , Romeo Catalin Cretu 3 , Raluca Florentina Cretu 4 , Alina Butu 1 1 National Institute of Research and Development for Biological Sciences, Bucharest, Romania 2 University of Agronomic Science and Veterinary Medicine, Bucharest, Romania; National Institute of Research and Development for Biological Sciences, Bucharest, Romania 3 University of Agronomic Science and Veterinary Medicine, Bucharest, Romania 4 The Bucharest University of Economic Studies, Romania
E-mail address: marian [email protected] (M. Butu). Defensins are the most known antimicrobial peptides synthesized by plants. Dynamics of the defensins is directly related with their mechanisms of action and the expression of antimicrobial activity. The aim of this research was to analyze the molecular dynamics of the defensin from germinated lentil (Lens culinaris) seeds and to study the potential biological activity. The structures of the defensin sequences were downloaded from RCSB Protein Data Bank. The simulations were performed in NPT ensemble with periodic boundary conditions. The simulation was analyzed with Gromacs package and VMD program. The analysis of trajectories was concentrated on dynamics of the disulfide bridges at various values of simulation temperature, between 300 and 500 K. The analyzed parameters were RMS, accessibility surface area, dihedrals and distances between C␣ atoms. Acknowledgements: This work has been financed by UEFISCDI, research contract PN-II-PT-PCCA 106/2012.
E-mail address: [email protected] (D. A. Dorottya). In June 2007, the European Council of Agriculture Ministers agreed on a new Council Regulation which contained objectives, clearly defined principles and general rules on organic production. In this Regulation was defined when the food can be labeled as “organic”. The rule is: food may be labeled as “organic” only if at least 95%
http://dx.doi.org/10.1016/j.jbiotec.2015.06.032
impacted, it has a molecular attraction that resists a tendency to flow and the resistance to penetration is direct proportional to the projectile’s velocity. For a tissue which is primarily like water, the resistance to penetration is proportional to the velocity squared. The brain is included in this group of materials. This paper shows the experimental results of impact tests using different weapon systems and ballistic gelatin. Lethal and non-lethal ammunition were shot. Also, a laboratory test for an arrow ammunition was performed in order to evaluate the impact with human tissue. The gelatin is widely used in these experiments because it is agreed to similarly respond to living tissue, without bones and muscles. The 20% (by mass) ballistic gelatin used in tests was made according to a NATO Allied Engineering Publication, Skin penetration assessment of non-lethal projectiles. Further, computer simulations of impact in gelatin were developed, both for reproducing the real tests and to analyze new loading cases. They give strong perspectives about tissue behavior and protective requirements. http://dx.doi.org/10.1016/j.jbiotec.2015.06.026
Oral Presentation Texts Using molecular tools to investigate the epidemiology of tick-borne diseases in Romania Mariana Ionita 1,∗ , Cornelia Silaghi 2 , Ioan Liviu Mitrea 1 , Kurt Pfister 2 1 Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agronomic Science and Veterinary Medicine, Bucharest, Romania 2 Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München, Germany
E-mail address: [email protected] (M. Ionita). In recent years, there has been increasing concern about the global spread of parasitic arthropods and the pathogens they transmit. Ticks are recognized among the most important vectors of diseases affecting both humans and animals. Ticks carry a wide range of pathogens (bacteria, viruses, protozoan) with an impact on animal and human health; some of these are recognized as important (re)emerging pathogens. The knowledge of pathogens transmitted by ticks in a given area is useful for assessing the risk of infection in humans and animals. One of the first steps for tick-borne disease risk assessment is the detection of the causing pathogens in their vectors. PCR amplification of pathogen DNA using species-specific primers is the standard for pathogen detection in ticks. In Romania, there is a very diverse ixodid tick fauna. Therefore, to assess the implication of ticks in the epidemiology of tick-borne diseases in Romania, a total of 619 ticks, including questing ticks and ticks collected from natural infected domestic animals (cattle, sheep, horses, dogs), were molecularly screened for tick-borne pathogens (TBP) using conventional PCR followed by sequencing or RealTime PCR. Tick species analyzed encompassed six species from different genera (Ixodes ricinus, Dermacentor marginatus, Dermacentor reticulatus, Rhipicephalus sanguineus sensu lato, Rhipicephalus bursa, Hyalomma marginatum). The results showed a wide distribution of very diverse bacterial (Rickettsia spp., Anaplasma phagocytophilum, Borrelia afzelii, Ehrlichia canis) and protozoan (Babesia spp., Theileria spp.) pathogens. These findings emphasize real risks for animal and human health in Romania and expand the known distribution of TBP in Europe. http://dx.doi.org/10.1016/j.jbiotec.2015.06.027
S13
Genetic parameters assessment in a Leghorn hen line Iuliana Neagu ∗ , Magda Gonciarov, Carmen Daniela Petcu, Margarita Oana Ghimpeteanu Department of Animal Production and Public Health, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania E-mail address: iuliana [email protected] (I. Neagu). The correct management of the breeding programs supposes the assessment of the genetic parameters in every generation of that population. The aim of the research consisted in the assessment of the genetic parameters of a Leghorn line by Restricted Maximum Likelihood method which assures positive estimates. There were sampled data for the 2014 generation regarding the number of eggs, egg weight at 28 weeks, egg weight at 34 weeks, body weight and age of the first egg. For variance-covariance analyze a linear mixed model was used (yij = + Ai + Cij ). The heritability was calculated as a ratio between the additive genetic variance and the total phenotypic variance. The results considered that the egg weight at 28 weeks (h2 = 0.216), the egg weight at 34 weeks (h2 = 0.214) and the body weight (h2 = 0.226), being considered intermediary transmitting characters. The number of eggs recorded a value of h2 = 0.036 and the age of the first egg h2 = 0.054, these characters being low heritable. The phenotypic correlation between the egg number and the age of first egg (rF = −0.423), is a very strict negative value and reveals the fact that hens which started laying eggs early are also good egg producers. The genotypic correlation between the egg number and the age of first egg (rG = 0.552) was very strict positive which shows that very early hens are not good egg producers, so a correlated for these characters selection could not be applied.
http://dx.doi.org/10.1016/j.jbiotec.2015.06.028 Production of extracellular alkaline protease by new halotolerant alkaliphilic Bacillus sp. NPST-AK15 isolated from hyper saline soda lakes Abdelnasser Salah Shebl Ibrahim ∗ , Ali A. Al Salamah, Yahya B. El Badawil, Mohamed A. El Tayeb Department of Botany and Microbiology, College of Science, King Saud University, Riyadh Saudi Arabia E-mail address: [email protected] (A.S.S. Ibrahim). Alkaline proteases are among the most important classes of industrial hydrolytic enzymes. The industrial demand for alkaline proteases with favorable properties continues to enhance the search for new enzymes. A new potent alkaline protease producing halotolerant alkaliphilic bacterium termed NPST-AK15, was isolated from hypersaline soda lakes, which affiliated to Bacillus sp strain NPST-AK15 based on 16S rRNA gene analysis (accession no. KP295749). Maximal protease production was obtained using yeast extract and fructose as nitrogen and carbon source, respectively. Strain NPST-AK15 can grow over a wide range of NaCl concentrations (0–20%), showing maximal growth and enzyme production at 0–5%, indicated the halotolerant nature of this bacterium. Ba and Ca ions enhanced enzyme production by about 1.6 and 1.3-fold respectively. The optimum temperature and pH for both enzyme production and cell growth was seen at 40 ◦ C and pH 11, respectively. Alkaline protease secretion was coherent with the growth
S14
Abstracts / Journal of Biotechnology 208 (2015) S5–S120
pattern, started at beginning of the exponential phase and reached maximal in mid stationary phase (36 h). Briefly, optimization of various fermentation parameters resulted in an increase of enzyme yield by 22.8-fold. NPST-AK15 and its extracellular alkaline protease with salt tolerance signify their potential applications in detergent formulations and other industries. http://dx.doi.org/10.1016/j.jbiotec.2015.06.029 Comparison of aroma production from castor oil by Yarrowia lipolytica in airlift and STR bioreactors Isabel Belo 1,∗ , Adelaide Braga 1 , Daniela Mesquita 1 , Luis Amaral 2 , Eugénio Ferreira 1 1
CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal 2 Instituto Politécnico de Coimbra, ISEC, DEQB, Coimbra, Portugal E-mail address: [email protected] (I. Belo). In aerobic cultures using hydrophobic substrates, such as the use of castor oil (CO) for ␥-decalactone production by Yarrowia lipolytica, the selection of bioreactor type is particularly important. Although stirred tanks (STR) are the most common industrial bioreactors used for aerobic fermentations, agitation conditions tend to cause some shearing stress to the cells. This can be prevented using aeration as driving force to promote agitation that is the principle of airlift reactors. This work analyses the influence of agitation (mechanical or pneumatic) and oxygen mass transfer coefficient (kLa) on ␥decalactone production by Y. lipolytica in a biphasic culture medium with CO as substrate. Y. lipolytica has the ability to perform the biotransformation of ricinoleic acid (the major fatty acid of CO), into ␥-decalactone through peroxisomal -oxidation. For both bioreactors ␥-decalactone productivity increased with kLa, in spite of the lower ␥-decalactone maximum concentration. On the other hand, higher oxygen transfer rate favored the accumulation of other lactone, such as 3-hydroxy-␥-decalactone. Higher values of ␥-decalactone concentration were obtained in the airlift (up to 3 g/L) compared to STR (up to 1.5 g/L). Also, the impact of agitation type in cell morphology was investigated using image analysis. Significant differences were observed between cellular populations of both bioreactors; in STR an increase in loose cells and quite irregular structures was observed, indicating that pneumatic agitation has less impact in cells morphology than mechanical agitation. http://dx.doi.org/10.1016/j.jbiotec.2015.06.030 A web-based application to inform consumers about the products based on corn and soybeans sold in Romania Domokos Alice Dorottya University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur 3-5, 400372, Cluj-Napoca, Romania
of their agricultural ingredients are organic. The use of genetically modified organisms (GMOs) and products derived from GMOs is still prohibited in the organic production. The products containing GMOs cannot be labeled as organic unless the ingredients containing GMOs have reached unintentionally in the product and if the proportion of GMOs in the ingredient is below 0.9%. The purpose of this paper is to present an interdisciplinary research by applying modern methods of computer science and molecular biology to inform the consumers about the products sold in Romania. To implement this, we creating a graphic interface, written in a component-based programming language PHP, and the information were stored in MySQL databases and it can be extracted to inform a consumer about the stored products. The database was populated with products based on corn and soybeans containing or not containing genetically modified organisms. The database also contained legislation on the marketing of these products. Products entered in the database have been tested in the laboratory “CERT-GMO” of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. The result of this process was a useful database, which contain information about products based on soy and maize sold in Romania. http://dx.doi.org/10.1016/j.jbiotec.2015.06.031 Analysis of the dynamics of the defensin from germinated lentil seeds Marian Butu 1,∗ , Steliana Rodino 2 , Marius Mihai Micu 3 , Petruta Petrache 3 , Romeo Catalin Cretu 3 , Raluca Florentina Cretu 4 , Alina Butu 1 1 National Institute of Research and Development for Biological Sciences, Bucharest, Romania 2 University of Agronomic Science and Veterinary Medicine, Bucharest, Romania; National Institute of Research and Development for Biological Sciences, Bucharest, Romania 3 University of Agronomic Science and Veterinary Medicine, Bucharest, Romania 4 The Bucharest University of Economic Studies, Romania
E-mail address: marian [email protected] (M. Butu). Defensins are the most known antimicrobial peptides synthesized by plants. Dynamics of the defensins is directly related with their mechanisms of action and the expression of antimicrobial activity. The aim of this research was to analyze the molecular dynamics of the defensin from germinated lentil (Lens culinaris) seeds and to study the potential biological activity. The structures of the defensin sequences were downloaded from RCSB Protein Data Bank. The simulations were performed in NPT ensemble with periodic boundary conditions. The simulation was analyzed with Gromacs package and VMD program. The analysis of trajectories was concentrated on dynamics of the disulfide bridges at various values of simulation temperature, between 300 and 500 K. The analyzed parameters were RMS, accessibility surface area, dihedrals and distances between C␣ atoms. Acknowledgements: This work has been financed by UEFISCDI, research contract PN-II-PT-PCCA 106/2012.
E-mail address: [email protected] (D. A. Dorottya). In June 2007, the European Council of Agriculture Ministers agreed on a new Council Regulation which contained objectives, clearly defined principles and general rules on organic production. In this Regulation was defined when the food can be labeled as “organic”. The rule is: food may be labeled as “organic” only if at least 95%
http://dx.doi.org/10.1016/j.jbiotec.2015.06.032