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Production of fibronectin and laminin in trabecular meshwork cultures mediated by phagocytosis
Thursday, Sep 24, 1992 La PalmalA
X ICER Abstracts
527
4 PRODUCTION OF FIBRONECTIN AND LAMININ IN TPABECUIAR MESHWORK CULTURES MRDIATED BY PRAGOCYTOSIS Bstrice Yue. I=abel -Jeana. w Department of Sciences, University of Illinois and Department of Ophthalmology,* Michigan, Ann Arbor, Michigan.
Eve Ophthalmology at Chicago, University
J.
and Visual Illinois, of
Trabecular meshwork cells are avid phagocytes. Using cultured bovine cells, we investigated the production of two cell attachment proteins, fibronectin and laminin, following phagocytosis. Cells were exposed to latex beads for 30 hr and the subsequent fibronectin and laminin production was measured by enzyme-linked immunosorbent assay. The amount of fibronectin associated with trabecular meshwork cells was reduced 4 and 8 hr after phagocytosis. Fibronectin, however, was returned to its normal value by 12 hr, and the normal level was retained through the 24-, 4S-, and 72-hr Similar changes time points. in laminin were also demonstrated. In addition, the. phagocytosed cells were less resistant than the control cells to trypsinization and resuspension from culture flasks at the 4- and S-hr time points. In vim, it has been shown that after phagocytosis, trabecular meshwork cells would round up, detach from trabecular beams, and migrate away. Our results suggest that such events may be a consequence of reduced cell-matrix cohesiveness related to the diminished levels of cell attachment proteins.
s.157
X ICER Abstracts
527
4 PRODUCTION OF FIBRONECTIN AND LAMININ IN TPABECUIAR MESHWORK CULTURES MRDIATED BY PRAGOCYTOSIS Bstrice Yue. I=abel -Jeana. w Department of Sciences, University of Illinois and Department of Ophthalmology,* Michigan, Ann Arbor, Michigan.
Eve Ophthalmology at Chicago, University
J.
and Visual Illinois, of
Trabecular meshwork cells are avid phagocytes. Using cultured bovine cells, we investigated the production of two cell attachment proteins, fibronectin and laminin, following phagocytosis. Cells were exposed to latex beads for 30 hr and the subsequent fibronectin and laminin production was measured by enzyme-linked immunosorbent assay. The amount of fibronectin associated with trabecular meshwork cells was reduced 4 and 8 hr after phagocytosis. Fibronectin, however, was returned to its normal value by 12 hr, and the normal level was retained through the 24-, 4S-, and 72-hr Similar changes time points. in laminin were also demonstrated. In addition, the. phagocytosed cells were less resistant than the control cells to trypsinization and resuspension from culture flasks at the 4- and S-hr time points. In vim, it has been shown that after phagocytosis, trabecular meshwork cells would round up, detach from trabecular beams, and migrate away. Our results suggest that such events may be a consequence of reduced cell-matrix cohesiveness related to the diminished levels of cell attachment proteins.
s.157