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Protective effects of beehive products and lactulose on chlorpromazine-induced liver damage in rats
Addendum ~ 6 " ~ COMPARISON OF SURGERY AND CHEMOTHERAPY IN ALVEOLAR ECHINOCOCCOSIS
Addendum
J. Watelet 1, S. Bresson-Hadni 2, F. Guillemin I , G. Sauve, I. Beurton 2, J.-P. Bronowicki 1. 1HGE, CHU, Nancy; 2HGE, CHU, Besancon, France The place of surgery and benzimidazole compounds in Echinococcus multilocularis is debated. We have investigated therapeutic and prognosis in 110 patients divided into 4 groups: Group A (n = 10) with small lesions and Group B (n = 50) with nonresectable lesions received exclusively mebendazole or/and albendazole. Group C (n = 18) could be cured by complete resection and Group D (n = 32) had a palliative surgery combined with chemotherapy. The criteria for failure were: complications, progression or recurrence, need to change treatment. These 4 groups did not differ significantly with regard to age, sex, origin, duration of chemotherapy. The number of complications was respectively 1/9 (group A), 19/50 (group B), 4/18 (group C), 19/32 (group D), p < 0.0001. Result of treatment was classified as improvement in 6 cases (group A), 32 cases (group B), 15 cases (group C) and in 12 cases (group D), p = 0.01. In spite of significant differences in hepatic lesions, vascular and biliary involvement, the survival mean (16 years for A, 13 years for B, 16 years for C and 19 years for D) and survival probability (A: 71%, B: 74%, C: 86%, D: 84% at 15 years) did not significantly differ in the four groups. Age and institution was able to infuence the survival level in univariate analysis and not significative in multivariate analysis. These results suggest a comparable efficacy of chemotherapy compared to surgery.
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PROTECTIVE EFFECTS OF BEEHIVE PRODUCTS AND LACTULOSE ON CHLORPROMAZINE-INDUCED LIVER DAMAGE IN RATS
D. Uzbekova, V. Makarova, L. Khvoynitskaya, L. Mirgorodskaya. Medical University, Ryazan, Russia The most serious adverse effect of chlorpromazine (Ch) is toxic hepatitis. Aim: To investigate the hepatoprotective effects of beehive product "Apiphytotonus" (AT) (2% royal jelly + 20% bee-collected pollen + 78% honey) and Lactulose (L) on chlorpromazine-induced liver damage. Methods: Ch (20 mg/kg/day) was given to male rats per os for 10 days. AT (500 mg/kg) or L (Inalco, Italy) (5 ml/kg/day) were given per os 10 days prior to Ch administration and during 10 days with Ch. The blood serum and hepatic homogenate were used to determine liver function, lipid peroxidation (LPO), sulfhydryl groups (SH-G) and glycogen contents and ammonia level (AL). Results: Ch intoxication resulted in marked LPO increase (expressed as MDA) and SH-G (p<0,001) and glycogen (p<0,05) decrease in liver; in blood serum alkaline phosphatase (AP) (p<0,001), ALT (p<0,001) and AL (p<0,001) increased. AT treatment of Ch-intoxicated rats normalised glycogen and SH-G contents in liver, MDA (p<0,05) and AL (p<0,05) partially decreased, ALT (p<0,001) and AP (p<0,001) activities markedly decreased. L treatment effectively reduced AL (by 3 fold) and MDA (p<0,001). Conclusions: AT having many active ingredients and natural antioxidants (glutathione, flavonoids, tocopherol, free SH-G) may protect Ch damage of liver. L may be useful in removing high AL, which has been formed during Ch intoxication and may be responsible for the induction of LPO.
Addendum
J. Watelet 1, S. Bresson-Hadni 2, F. Guillemin I , G. Sauve, I. Beurton 2, J.-P. Bronowicki 1. 1HGE, CHU, Nancy; 2HGE, CHU, Besancon, France The place of surgery and benzimidazole compounds in Echinococcus multilocularis is debated. We have investigated therapeutic and prognosis in 110 patients divided into 4 groups: Group A (n = 10) with small lesions and Group B (n = 50) with nonresectable lesions received exclusively mebendazole or/and albendazole. Group C (n = 18) could be cured by complete resection and Group D (n = 32) had a palliative surgery combined with chemotherapy. The criteria for failure were: complications, progression or recurrence, need to change treatment. These 4 groups did not differ significantly with regard to age, sex, origin, duration of chemotherapy. The number of complications was respectively 1/9 (group A), 19/50 (group B), 4/18 (group C), 19/32 (group D), p < 0.0001. Result of treatment was classified as improvement in 6 cases (group A), 32 cases (group B), 15 cases (group C) and in 12 cases (group D), p = 0.01. In spite of significant differences in hepatic lesions, vascular and biliary involvement, the survival mean (16 years for A, 13 years for B, 16 years for C and 19 years for D) and survival probability (A: 71%, B: 74%, C: 86%, D: 84% at 15 years) did not significantly differ in the four groups. Age and institution was able to infuence the survival level in univariate analysis and not significative in multivariate analysis. These results suggest a comparable efficacy of chemotherapy compared to surgery.
165
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PROTECTIVE EFFECTS OF BEEHIVE PRODUCTS AND LACTULOSE ON CHLORPROMAZINE-INDUCED LIVER DAMAGE IN RATS
D. Uzbekova, V. Makarova, L. Khvoynitskaya, L. Mirgorodskaya. Medical University, Ryazan, Russia The most serious adverse effect of chlorpromazine (Ch) is toxic hepatitis. Aim: To investigate the hepatoprotective effects of beehive product "Apiphytotonus" (AT) (2% royal jelly + 20% bee-collected pollen + 78% honey) and Lactulose (L) on chlorpromazine-induced liver damage. Methods: Ch (20 mg/kg/day) was given to male rats per os for 10 days. AT (500 mg/kg) or L (Inalco, Italy) (5 ml/kg/day) were given per os 10 days prior to Ch administration and during 10 days with Ch. The blood serum and hepatic homogenate were used to determine liver function, lipid peroxidation (LPO), sulfhydryl groups (SH-G) and glycogen contents and ammonia level (AL). Results: Ch intoxication resulted in marked LPO increase (expressed as MDA) and SH-G (p<0,001) and glycogen (p<0,05) decrease in liver; in blood serum alkaline phosphatase (AP) (p<0,001), ALT (p<0,001) and AL (p<0,001) increased. AT treatment of Ch-intoxicated rats normalised glycogen and SH-G contents in liver, MDA (p<0,05) and AL (p<0,05) partially decreased, ALT (p<0,001) and AP (p<0,001) activities markedly decreased. L treatment effectively reduced AL (by 3 fold) and MDA (p<0,001). Conclusions: AT having many active ingredients and natural antioxidants (glutathione, flavonoids, tocopherol, free SH-G) may protect Ch damage of liver. L may be useful in removing high AL, which has been formed during Ch intoxication and may be responsible for the induction of LPO.