- Email: [email protected]
Protective effects of catechin, morin, myricetin, kaempferol, luteolin and naringin on human LDL carbamylation in vitro
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Abstracts
E Poster – [A-10-360-1] Alcoholic garlic (Allium sativum) extract increases ABCA1 mRNA expression in human THP-1 macrophages Malekpour-Dehkordi Zahra, Javadi Ebrahim, Doosti Mahmood, Paknejad Maliheh, Nourbakhsh Mitra, Yassa Narguess, Gerayesh-Nejad Siavash Tehran University of Medical Sciences, Tehran,Iran E-mail addresses: [email protected] (M.-D. Zahra), [email protected] (J. Ebrahim), [email protected] (D. Mahmood), [email protected] (P. Maliheh), [email protected] (N. Mitra), [email protected] (Y. Narguess), [email protected] (G.-N. Siavash) High density lipoprotein and apo A-I are major constituents in reverse cholesterol transport, a pathway by which accumulated cholesterol is transported from the vessel wall macrophages to the liver for excretion, thus preventing atherosclerosis. Recent studies have demonstrated that ATP-binding cassette transporter A1 (ABCA1) mediates the efflux of cholesterol and phospholipids to apolipoproteins, a process necessary for HDL formation. In the other hand, garlic (Allium sativum) and garlic extracts have been demonstrated to reduce the parameters associated with cardiovascular disease. Addition, garlic has antiatherogenic and antiathersclerotic properties. The goal of this study was to determine the effect of alcoholic garlic (Allium sativum) extract on the expression of ABCA1 mRNA in macrophages. The human monocyte THP-1 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS). The THP-1 monocyte cells were differentiated to macrophage using phorbol 12-myristate 13acetate. In order to determine an LC50 of alcoholic garlic extract on THP-1 macrophages MTT assay was used. Macrophage cells were treated with different concentrations of alcoholic garlic extract. Total RNA of treated macrophages was extracted and analyzed with real-time PCR. Results showed that alcoholic garlic extract increased the ABCA1 mRNA expression in macrophage THP1 cells (20–23%) compared with control (untreated cells). Results suggested that alcoholic garlic extract has potential effect on increasing ABCA1 expression in macrophages and possibly promote reverse cholesterol efflux. Keywords: Alcoholic garlic extract, ATP-binding cassette transporter A1
doi:10.1016/j.clinbiochem.2011.08.180
E Poster – [A-10-533-1] The assessment of biochemical parameters of iron metabolism and heme/non-heme iron intake in CAD patients in comparison with controls Tayebeh Zohrabia, Ahmad Movahedianb, Zamzam Paknahadc, Mohamad Hashemi Jazid,1, Mohammad Reza Maracye,2 a School of Pharmacy, Isfahan University of Medical Science, Isfahan, Iran b Department of Clinical Biochemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran c Department of Nutrition, Health Faculty, Medical Sciences University, Isfahan, Iran d Noor Hospital, Hasht Behesht Bulvar, Isfahan, Iran e Department of Epidemiology and Biostatistics, School of Health, Isfahan University of Medical Sciences, Iran E-mail addresses: [email protected] (T. Zohrabi), [email protected] (A. Movahedian),
[email protected] (Z. Paknahad), [email protected] (M.H. Jazi), [email protected] (M.R. Maracy) 1 Tel.: +98 311 2222255 (office). 2 Tel.: +98 311 7922773 (office). Introduction: The association between body iron stores level and coronary artery disease (CAD) is debated. It has been suggested that ferrous iron Fe(ΙΙ) can catalyze the production of reactive oxygen species and may cause oxidative damage. However the role of dietary factors such as iron intake in increasing iron stores is unknown. Thus, we investigated the relationship between body iron stores and dietary iron intake in CAD patients in comparison with controls. Methods: In this case–control study, 140 male patients (age ≥ 45 years) that referred for diagnostic coronary angiography were divided into two groups according to their angiographic results: a case group with ≥ 75% and a control group with < 75% stenosis in one of the coronary arteries. Exclusion criteria were BMI < 18.5 and > 29.9 kg/m2, inflammatory and chronic disease, hemochromatosis and iron supplement consumption. Blood sampling was performed before angiography and dietary information was collected by a food–frequency questionnaire. Results: Mean iron stores concentration were higher in the case group than control groups [ferritin (129.8 ± 99 vs 107.7 ± 75 ng/ml), transferrin (288.8 ± 53.9 vs 285 ± 58.9 μg/dl)], however these differences were not statistically significant; dietary mean iron intakes were significantly higher in the case group [total iron (21.1 ± 10.9 vs 16 ± 6.1), heme iron (3.1 ± 3 vs 2.2 ± 1.8) and non heme iron (17.9 ± 10.3 vs 13.7 ± 5.2)] (P = 0.011, P = 0.044, P = 0.014 ,respectively). Conclusions: Consumption of an iron rich diet could stimulate oxidative stress by increasing iron stores and development of coronary artery disease in men. Keywords: CAD, Ferritin, Transferring, Heme iron, Non heme iron, Angiography
doi:10.1016/j.clinbiochem.2011.08.181
E Poster – [A-10-568-1] Protective effects of catechin, morin, myricetin, kaempferol, luteolin and naringin on human LDL carbamylation in vitro Ghaffari Mohammad Ali, Shanaki Mehrnosh Ahwaz Jundishapur University of Medical Sciences, Ahvaz, Iran E-mail addresses: [email protected] (G.M. Ali), [email protected] (S. Mehrnosh) Introduction: Previous studies have shown that increase of carbamylated LDL (cLDL), a product of nonenzymatic modification of LDL in human serum by urea-derived cyanate, may cause cardiovascular complications in patients with chronic renal insufficiency. This study examined the inhibitory effects of catechin, morin, myricetin, kaempferol, luteolin and naringin on LDL carbamylation in an in vitro model. Methods: After isolation of LDL from plasma using ultracentrifuge technique, cyanate was added to it and then LDL carbamylation was estimated in absence and presence of catechin, morin, myricetin, kaempferol, luteolin and naringin by a colorimetric method at 530 nm. Results: We showed that six flavonoids, catechin, morin, myricetin, kaempferol, luteolin and naringin, inhibit LDL carbamylation, that the most effect was related to catechin. Conclusion: This study indicate that catechin, morin, myricetin, kaempferol, luteolin and naringin probably with antioxidant activity can inhibit LDL carbamylation and thus may have a role in ameliorating atherosclerotic risk of patient with kidney failure. However, further investigations as in vitro and in vivo, are needed to elucidate the exact effect of these flavonoids on uremic patients.
Abstracts
Keywords: Carbamylation, Catechin, Morin, Myricetin, Kaempferol, Luteolin, Naringin, LDL doi:10.1016/j.clinbiochem.2011.08.182
E Poster – [A-10-591-1] Changes of urinary iodine and thyrotropin during pregnancy in an iodine deficient area Beiranvand Asghara, Rostami Rahima, Salarilak Shakerb, Aghassi Mohammad Rezaa, Khalkhali Hamid Rezaa, Nouroozzadeh Jafara a Medical Faculty, Naslu-Urmia, West Azarbaijan, Iran b Medicine Faculty, Azad University, Tabriz, East Azarbaijan, Iran E-mail addresses: [email protected] (B. Asghar), [email protected] (R. Rahim), [email protected] (S. Shaker), [email protected] (A.M. Reza), [email protected] (K.H. Reza), [email protected] (N. Jafar) Introduction: Iodine is an essential component for proper thyroid function. In addition, its adequate availability during pregnancy is highly important for normal brain development of the fetus. Urinary iodine excretion (UIE) and thyrotropin (TSH) are indices of iodine intake and thyroid function. To assess iodine intake (I/Cr ratio) and thyroid function of pregnant women in Urmia city—West Azarbaijan province. Methods: 1078 pregnant women, referred to their 1st planned care, were interviewed at health centers (urban and amp; rural parts). Of these, 490 subjects were enrolled. Sera and urine samples were collected at two stages (1st and amp; 3rd trimesters). Urinary iodine excretion (UIE), urinary creatinine(Cr), TSH, and thyroproxidase antibody(TPO Ab) were measured. Results: Median I/Cr ratio was lower at 1st trimester than at 3rd trimester (46.4 vs.113.4, p< 0.0001). Mean(SD) of TSH(log-transformed) was lower at 1st trimester than at 3rd trimester also ( −0.15(0.48) vs. 0.23(0.32) IU/mL, p<0.001). The respected values for TPO Ab were 0.38 (0.94) and 0.25(0.79) IU/mL in order which showed significant increase (p <0.001). Subjects with positive TPO Ab were 10% at 1st trimester which reduced to 5% at 3rd trimester. Conclusion: our data reveals that a mild-moderate iodine deficiency is present among pregnant women in Urmia. The figures for TSH at the 1st trimester emphasize the need for a definitive reference range of TSH. Further studies are needed to address the reasons for the low iodine despite two decades after salt enrichment in Iran. Keywords: Iodine, TSH, Pregnancy, TPO antibody doi:10.1016/j.clinbiochem.2011.08.183
E Poster – [A-10-621-1] Effect of tomato juice on serum concentrations of IL-6, IL-8, CRP and TNF-α in female students' overweight or obese dormitory of Tehran University of Medical Sciences Ghavipour Mahsa, Saedi Ahmad, Djallali Mahmoud, Eshraghiyan Mohammad Reza School of Public Health, Tehran University of Medical Sciences, Iran E-mail addresses: [email protected] (G. Mahsa), [email protected] (S. Ahmad), [email protected] (D. Mahmoud), [email protected] (E.M. Reza) Introduction: the tomato and its products are the richest source of lycopene (the most powerful antioxidant among the carotenoids, and an anti-inflammatory factor). Since overweight and obesity
S81
increase the risk of inflammation and inflammatory diseases, this study is associated with the effect of tomato juice on serum concentrations of IL-6, IL-8, hsCRP, and TNF-α in overweight female students' weight or obese Tehran University dormitory. Method: In a clinical trial 118 overweight or obese individuals were chosen among female students at the dormitory of Tehran Medical Sciences University, and randomly selected 59 groups of people under control and intervention groups. The intervention group, received 300 ml of tomato juice (37 mg lycopene per day) to 20 days. Before and after the intervention period serum concentrations of hsCRP, IL-6, IL-8 and TNF-α in the two groups were compared. Results: Serum concentrations of IL-8 in the intervention group (from 129.26 to 119.35) was significantly decreased and in the control group (from 127.56 to 133.96) was increased. The difference in concentrations of IL-8 (intervention group: 9.91 and controls: 6.39) and TNF-α (intervention group: 8.25 and control group: 0.35) before and after intervention in both groups was significant. Changes in other variables were not significant. Discussion: The findings indicate that tomato juice may reduce inflammation in overweight and obese effectively. Keywords: Lycopene, Inflammatory biomarkers, Obesity, Inflammation
doi:10.1016/j.clinbiochem.2011.08.184
E Poster – [A-10-708-3] The effect of sour cherry juice on total antioxidant activity and testosterone concentrations in cigarette smoking men Khadem Ansari Mohammad Hassana, Shirpoor Rasoolb, Rasmi Yousefa, Shasavari Zahraa a Faculty of Medicine, Urmia University of Medical Science b Payame Noor University Tehran E-mail addresses: [email protected] (K.A.M. Hassan), [email protected] (S. Rasool), [email protected] (R. Yousef), [email protected] (S. Zahra) Introduction: Antioxidants are important groups of chemical compounds that protect body against destructive factors such as free active radicals resulted from food metabolism. To encounter by these destructive factors, body system produce some antioxidants like enzymes and hormones and some other antioxidants through regimen. Examination of sour cherry juice effect on Total Antioxidants (TAC) and testosterone hormone secretion in men addicted to smoking cigarettes and its comparison with addicted men without taking juice were studied. Method and materials: For measuring TAC, method by the use of Randox kit is applied and for testosterone measurement, direct competitive method of luminance (solid phase) by using Liasian , an automatic system, is employed.. Towenty five volunteers of male employes of Shadlee Company addicted to smoking cigarettes were registered and received 2 glasses ≈300 cc concentrated sour cherry juice daily, for one month, and also 25 persons of company's employees that also smoking cigarettes were chosen as a control group that they did not use sour cherry juice. After a month, five milliliter blood were taken and serum after 15 minutes centrifuge in room temperature is isolated for examination. Results: This research shows that drinking 300 cc sour cherry juice daily has a positive effect on TAC in people who addicted to smoking cigarettes(Pvalve = 0.000) but no significant change in testosterone hormone were observed. There is no significant changes in TAC and testosterone in control groups. Conlusion: Sour cherry shows increase in TAC level in cigarette smoking men but no effect on testosterone level.
Abstracts
E Poster – [A-10-360-1] Alcoholic garlic (Allium sativum) extract increases ABCA1 mRNA expression in human THP-1 macrophages Malekpour-Dehkordi Zahra, Javadi Ebrahim, Doosti Mahmood, Paknejad Maliheh, Nourbakhsh Mitra, Yassa Narguess, Gerayesh-Nejad Siavash Tehran University of Medical Sciences, Tehran,Iran E-mail addresses: [email protected] (M.-D. Zahra), [email protected] (J. Ebrahim), [email protected] (D. Mahmood), [email protected] (P. Maliheh), [email protected] (N. Mitra), [email protected] (Y. Narguess), [email protected] (G.-N. Siavash) High density lipoprotein and apo A-I are major constituents in reverse cholesterol transport, a pathway by which accumulated cholesterol is transported from the vessel wall macrophages to the liver for excretion, thus preventing atherosclerosis. Recent studies have demonstrated that ATP-binding cassette transporter A1 (ABCA1) mediates the efflux of cholesterol and phospholipids to apolipoproteins, a process necessary for HDL formation. In the other hand, garlic (Allium sativum) and garlic extracts have been demonstrated to reduce the parameters associated with cardiovascular disease. Addition, garlic has antiatherogenic and antiathersclerotic properties. The goal of this study was to determine the effect of alcoholic garlic (Allium sativum) extract on the expression of ABCA1 mRNA in macrophages. The human monocyte THP-1 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS). The THP-1 monocyte cells were differentiated to macrophage using phorbol 12-myristate 13acetate. In order to determine an LC50 of alcoholic garlic extract on THP-1 macrophages MTT assay was used. Macrophage cells were treated with different concentrations of alcoholic garlic extract. Total RNA of treated macrophages was extracted and analyzed with real-time PCR. Results showed that alcoholic garlic extract increased the ABCA1 mRNA expression in macrophage THP1 cells (20–23%) compared with control (untreated cells). Results suggested that alcoholic garlic extract has potential effect on increasing ABCA1 expression in macrophages and possibly promote reverse cholesterol efflux. Keywords: Alcoholic garlic extract, ATP-binding cassette transporter A1
doi:10.1016/j.clinbiochem.2011.08.180
E Poster – [A-10-533-1] The assessment of biochemical parameters of iron metabolism and heme/non-heme iron intake in CAD patients in comparison with controls Tayebeh Zohrabia, Ahmad Movahedianb, Zamzam Paknahadc, Mohamad Hashemi Jazid,1, Mohammad Reza Maracye,2 a School of Pharmacy, Isfahan University of Medical Science, Isfahan, Iran b Department of Clinical Biochemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran c Department of Nutrition, Health Faculty, Medical Sciences University, Isfahan, Iran d Noor Hospital, Hasht Behesht Bulvar, Isfahan, Iran e Department of Epidemiology and Biostatistics, School of Health, Isfahan University of Medical Sciences, Iran E-mail addresses: [email protected] (T. Zohrabi), [email protected] (A. Movahedian),
[email protected] (Z. Paknahad), [email protected] (M.H. Jazi), [email protected] (M.R. Maracy) 1 Tel.: +98 311 2222255 (office). 2 Tel.: +98 311 7922773 (office). Introduction: The association between body iron stores level and coronary artery disease (CAD) is debated. It has been suggested that ferrous iron Fe(ΙΙ) can catalyze the production of reactive oxygen species and may cause oxidative damage. However the role of dietary factors such as iron intake in increasing iron stores is unknown. Thus, we investigated the relationship between body iron stores and dietary iron intake in CAD patients in comparison with controls. Methods: In this case–control study, 140 male patients (age ≥ 45 years) that referred for diagnostic coronary angiography were divided into two groups according to their angiographic results: a case group with ≥ 75% and a control group with < 75% stenosis in one of the coronary arteries. Exclusion criteria were BMI < 18.5 and > 29.9 kg/m2, inflammatory and chronic disease, hemochromatosis and iron supplement consumption. Blood sampling was performed before angiography and dietary information was collected by a food–frequency questionnaire. Results: Mean iron stores concentration were higher in the case group than control groups [ferritin (129.8 ± 99 vs 107.7 ± 75 ng/ml), transferrin (288.8 ± 53.9 vs 285 ± 58.9 μg/dl)], however these differences were not statistically significant; dietary mean iron intakes were significantly higher in the case group [total iron (21.1 ± 10.9 vs 16 ± 6.1), heme iron (3.1 ± 3 vs 2.2 ± 1.8) and non heme iron (17.9 ± 10.3 vs 13.7 ± 5.2)] (P = 0.011, P = 0.044, P = 0.014 ,respectively). Conclusions: Consumption of an iron rich diet could stimulate oxidative stress by increasing iron stores and development of coronary artery disease in men. Keywords: CAD, Ferritin, Transferring, Heme iron, Non heme iron, Angiography
doi:10.1016/j.clinbiochem.2011.08.181
E Poster – [A-10-568-1] Protective effects of catechin, morin, myricetin, kaempferol, luteolin and naringin on human LDL carbamylation in vitro Ghaffari Mohammad Ali, Shanaki Mehrnosh Ahwaz Jundishapur University of Medical Sciences, Ahvaz, Iran E-mail addresses: [email protected] (G.M. Ali), [email protected] (S. Mehrnosh) Introduction: Previous studies have shown that increase of carbamylated LDL (cLDL), a product of nonenzymatic modification of LDL in human serum by urea-derived cyanate, may cause cardiovascular complications in patients with chronic renal insufficiency. This study examined the inhibitory effects of catechin, morin, myricetin, kaempferol, luteolin and naringin on LDL carbamylation in an in vitro model. Methods: After isolation of LDL from plasma using ultracentrifuge technique, cyanate was added to it and then LDL carbamylation was estimated in absence and presence of catechin, morin, myricetin, kaempferol, luteolin and naringin by a colorimetric method at 530 nm. Results: We showed that six flavonoids, catechin, morin, myricetin, kaempferol, luteolin and naringin, inhibit LDL carbamylation, that the most effect was related to catechin. Conclusion: This study indicate that catechin, morin, myricetin, kaempferol, luteolin and naringin probably with antioxidant activity can inhibit LDL carbamylation and thus may have a role in ameliorating atherosclerotic risk of patient with kidney failure. However, further investigations as in vitro and in vivo, are needed to elucidate the exact effect of these flavonoids on uremic patients.
Abstracts
Keywords: Carbamylation, Catechin, Morin, Myricetin, Kaempferol, Luteolin, Naringin, LDL doi:10.1016/j.clinbiochem.2011.08.182
E Poster – [A-10-591-1] Changes of urinary iodine and thyrotropin during pregnancy in an iodine deficient area Beiranvand Asghara, Rostami Rahima, Salarilak Shakerb, Aghassi Mohammad Rezaa, Khalkhali Hamid Rezaa, Nouroozzadeh Jafara a Medical Faculty, Naslu-Urmia, West Azarbaijan, Iran b Medicine Faculty, Azad University, Tabriz, East Azarbaijan, Iran E-mail addresses: [email protected] (B. Asghar), [email protected] (R. Rahim), [email protected] (S. Shaker), [email protected] (A.M. Reza), [email protected] (K.H. Reza), [email protected] (N. Jafar) Introduction: Iodine is an essential component for proper thyroid function. In addition, its adequate availability during pregnancy is highly important for normal brain development of the fetus. Urinary iodine excretion (UIE) and thyrotropin (TSH) are indices of iodine intake and thyroid function. To assess iodine intake (I/Cr ratio) and thyroid function of pregnant women in Urmia city—West Azarbaijan province. Methods: 1078 pregnant women, referred to their 1st planned care, were interviewed at health centers (urban and amp; rural parts). Of these, 490 subjects were enrolled. Sera and urine samples were collected at two stages (1st and amp; 3rd trimesters). Urinary iodine excretion (UIE), urinary creatinine(Cr), TSH, and thyroproxidase antibody(TPO Ab) were measured. Results: Median I/Cr ratio was lower at 1st trimester than at 3rd trimester (46.4 vs.113.4, p< 0.0001). Mean(SD) of TSH(log-transformed) was lower at 1st trimester than at 3rd trimester also ( −0.15(0.48) vs. 0.23(0.32) IU/mL, p<0.001). The respected values for TPO Ab were 0.38 (0.94) and 0.25(0.79) IU/mL in order which showed significant increase (p <0.001). Subjects with positive TPO Ab were 10% at 1st trimester which reduced to 5% at 3rd trimester. Conclusion: our data reveals that a mild-moderate iodine deficiency is present among pregnant women in Urmia. The figures for TSH at the 1st trimester emphasize the need for a definitive reference range of TSH. Further studies are needed to address the reasons for the low iodine despite two decades after salt enrichment in Iran. Keywords: Iodine, TSH, Pregnancy, TPO antibody doi:10.1016/j.clinbiochem.2011.08.183
E Poster – [A-10-621-1] Effect of tomato juice on serum concentrations of IL-6, IL-8, CRP and TNF-α in female students' overweight or obese dormitory of Tehran University of Medical Sciences Ghavipour Mahsa, Saedi Ahmad, Djallali Mahmoud, Eshraghiyan Mohammad Reza School of Public Health, Tehran University of Medical Sciences, Iran E-mail addresses: [email protected] (G. Mahsa), [email protected] (S. Ahmad), [email protected] (D. Mahmoud), [email protected] (E.M. Reza) Introduction: the tomato and its products are the richest source of lycopene (the most powerful antioxidant among the carotenoids, and an anti-inflammatory factor). Since overweight and obesity
S81
increase the risk of inflammation and inflammatory diseases, this study is associated with the effect of tomato juice on serum concentrations of IL-6, IL-8, hsCRP, and TNF-α in overweight female students' weight or obese Tehran University dormitory. Method: In a clinical trial 118 overweight or obese individuals were chosen among female students at the dormitory of Tehran Medical Sciences University, and randomly selected 59 groups of people under control and intervention groups. The intervention group, received 300 ml of tomato juice (37 mg lycopene per day) to 20 days. Before and after the intervention period serum concentrations of hsCRP, IL-6, IL-8 and TNF-α in the two groups were compared. Results: Serum concentrations of IL-8 in the intervention group (from 129.26 to 119.35) was significantly decreased and in the control group (from 127.56 to 133.96) was increased. The difference in concentrations of IL-8 (intervention group: 9.91 and controls: 6.39) and TNF-α (intervention group: 8.25 and control group: 0.35) before and after intervention in both groups was significant. Changes in other variables were not significant. Discussion: The findings indicate that tomato juice may reduce inflammation in overweight and obese effectively. Keywords: Lycopene, Inflammatory biomarkers, Obesity, Inflammation
doi:10.1016/j.clinbiochem.2011.08.184
E Poster – [A-10-708-3] The effect of sour cherry juice on total antioxidant activity and testosterone concentrations in cigarette smoking men Khadem Ansari Mohammad Hassana, Shirpoor Rasoolb, Rasmi Yousefa, Shasavari Zahraa a Faculty of Medicine, Urmia University of Medical Science b Payame Noor University Tehran E-mail addresses: [email protected] (K.A.M. Hassan), [email protected] (S. Rasool), [email protected] (R. Yousef), [email protected] (S. Zahra) Introduction: Antioxidants are important groups of chemical compounds that protect body against destructive factors such as free active radicals resulted from food metabolism. To encounter by these destructive factors, body system produce some antioxidants like enzymes and hormones and some other antioxidants through regimen. Examination of sour cherry juice effect on Total Antioxidants (TAC) and testosterone hormone secretion in men addicted to smoking cigarettes and its comparison with addicted men without taking juice were studied. Method and materials: For measuring TAC, method by the use of Randox kit is applied and for testosterone measurement, direct competitive method of luminance (solid phase) by using Liasian , an automatic system, is employed.. Towenty five volunteers of male employes of Shadlee Company addicted to smoking cigarettes were registered and received 2 glasses ≈300 cc concentrated sour cherry juice daily, for one month, and also 25 persons of company's employees that also smoking cigarettes were chosen as a control group that they did not use sour cherry juice. After a month, five milliliter blood were taken and serum after 15 minutes centrifuge in room temperature is isolated for examination. Results: This research shows that drinking 300 cc sour cherry juice daily has a positive effect on TAC in people who addicted to smoking cigarettes(Pvalve = 0.000) but no significant change in testosterone hormone were observed. There is no significant changes in TAC and testosterone in control groups. Conlusion: Sour cherry shows increase in TAC level in cigarette smoking men but no effect on testosterone level.