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Protein involved in regenerating luciferin (N Kajiyama, JP)
The inventors W we 98/00532, PCT. The invention relates to oligonucleotides from the untranslated regions of housekeeping genes, and methods and compositions for modulating cell growth using same.
> Biosensor, method of
producing them and analysis methods using same (A Haouz et ai, FR) Zellweger Analytics SA et les inventeurs W we 98/02569, PCT. The invention concerns a biosensor comprising an enzymatic film and a transducer, characterised in that the film consists of a membrane with reagent groupings, at least one enzyme and two dialysis membranes. This bios~nsor has such stability that it can be used continuously for more than a month in an industrial analysing device mainly ensuring regeneration of the biosensor and the dosing parameter measurement.
> Multilayer enzyme electrode membranes and methods of making same (R McCaffrey et ai, US) Chiron Diagnostics Corp and the inventors W we 98/02736, PCT. A sensor for use in detecting a concentration of analyte in a sample solution. (... )
Analyse du genome > Method for sequencing nucleic acids using matrixassisted laser desorption ionization time-of-flight mass spectrometry (BH Wang and A Cohen, US Hybridon Inc W we 98/03684, PCT. > Method for contiguous genome sequencing (P Senapathy, US) The inventor N°we 98/02575, PCT. A new contiguous genome sequencing method is described which allows the contiguous sequencing of a very long DNA without need to be sucloned (... )
Animaux tra nsuentque s > Transgenic animal whose expression of the 48 BIOFUTUR 178 • Mai 1998
opiate receptor is modified (BLKieffer at ai, FR) Cnrs et les inventeurs W we 98/02534, peT. The invention concerns the use of a non-human transgenic mammal whose expression of a gene coding for an opiate receptor is modified, particularly in the nerve tissues with respect to a normal expression, in particular in the nerve tissues, for producing a medicine for the treatment of pathological conditions involving opiate receptors, in particular severe acute or chronic pains, drug addition or the prevention or the treatment of transplant rejections.
Plantes tr ansqenlque s > Controlled gene expression in plants (K Palme et ai, DE, UK) Max-Planck-Gesellschaft zur Forderung der Wissenchaften eV N°823480, EP. This invention relates to the control of gene expression in plants using either a chimeric transcriptional activator protein system, or a system based on suppressor tRNAs. (... )
Methodes d'amplification > Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific Tm (M Becker and M Majlessi, US) Gen-Probe Inc N° we 98/02582, PCT. The present invention concerns oligonucleotides containing one or more modified nucleotides which increased the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. (... )
Divers > Protein involved in re-
generating luciferin (N Kajiyama, JP)
Kikkoman Corp N°825257, EP. A protein having the ability to regenerate luciferin by acting on oxyluciferin and Dcysteine. By adding this protein to a luciferin/luciferase reaction system, the luminescence can persist and the amount of luciferase and luciferin used can be reduced.
>
Pressure~mediated binding of bion»leculer complexe. (OJ Litt at ai, US) Bioseq Inc and the inventors N" we 98/00032. PCT The invention relates to pressure-mediated dissociation of an analyte complexed with an endogenous binding partner to enable detection of a complex formed from the analyte and an exogenous binding factor, pressure-mediated association of an analyte and an exogenous binding partner to enable more rapid andlor more sensitive detection of an analyre, and pressure-mediated association and dissociation of biomolecular complexes to enable separation of one biomolecule from a complexe mixture. (... )
> High-throughput scree-
ning asay systems in mlcroscale fluidic devices (JW Paree et ai, US) Caliper Technologies Corp and the inventors N" WO 98/00231, PCT. The present invention provides microfluidic devices and methods that are useful for performing highthroughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems. (... )
> A recombinant enzyme
with mutlnase ~ (JA Wahleithner et ai, US, DK) Novo Nordisk NS and the inventors N°we 98/00528, PCT The present invention relates to method for constructing an expression vector comprising a rnutanase gene obtained from a filamentous fungus suitable for heterologous production cornpri-
sing the steps of isolating a DNA sequence encoding a mutanase from a filamentous fungus, introducing a kex2 or kex2-like site between the DNA sequences encoding the pro-peptide and the mature region of the mutanase, cloning the DNA sequence obtained in step into a suitable expression vector. (... )
> Composition and methods for modulating the length of telomeres (B Chabot, CAl Universita de Sherbrooke and the inventor N°we 98/00537, peT.
> Denaturation of double-
stranded nucleic acid (DC Purvis and S Watson, GB) Scientific Generics Ltd and the inventors N" we 98/00562, PCT. Double-stranded nucleic acid is denatured by subjecting a solution thereof to a voltage applied between electrodes spaced by no more than 1.5 mm in a time not previously achievable in electrochemical denaturation (... ) > Enzymatically removable linkers for solid phase synthesis (H Waldmann et ai, DE) BAF AG and the inventors N°we 98/01406, PCT > Determination of anaIytes by means of two markers (U Giesen et el, DE) Boehringer Mannheim GmbH and the inventors N" WO 98/01578, PCT. The combination of two detection mechanisms in a single apparatus leads to a process that enables the detected analytes to be easily quantified. Appropriate formats and reagent kits are disclosed. > Systeme de detection des interaction protelneprotelne (M Granger et M Schnarr, FR) Cnrs et les inventeurs N" we 98/01579, PCT. La presente invention concerne I'utilisation d'une sequence d'ADN cornprenant : une sequence nucleotidique codant une proteine indicatrice (gene indicateur), un prornoteur, un operareur
> Biosensor, method of
producing them and analysis methods using same (A Haouz et ai, FR) Zellweger Analytics SA et les inventeurs W we 98/02569, PCT. The invention concerns a biosensor comprising an enzymatic film and a transducer, characterised in that the film consists of a membrane with reagent groupings, at least one enzyme and two dialysis membranes. This bios~nsor has such stability that it can be used continuously for more than a month in an industrial analysing device mainly ensuring regeneration of the biosensor and the dosing parameter measurement.
> Multilayer enzyme electrode membranes and methods of making same (R McCaffrey et ai, US) Chiron Diagnostics Corp and the inventors W we 98/02736, PCT. A sensor for use in detecting a concentration of analyte in a sample solution. (... )
Analyse du genome > Method for sequencing nucleic acids using matrixassisted laser desorption ionization time-of-flight mass spectrometry (BH Wang and A Cohen, US Hybridon Inc W we 98/03684, PCT. > Method for contiguous genome sequencing (P Senapathy, US) The inventor N°we 98/02575, PCT. A new contiguous genome sequencing method is described which allows the contiguous sequencing of a very long DNA without need to be sucloned (... )
Animaux tra nsuentque s > Transgenic animal whose expression of the 48 BIOFUTUR 178 • Mai 1998
opiate receptor is modified (BLKieffer at ai, FR) Cnrs et les inventeurs W we 98/02534, peT. The invention concerns the use of a non-human transgenic mammal whose expression of a gene coding for an opiate receptor is modified, particularly in the nerve tissues with respect to a normal expression, in particular in the nerve tissues, for producing a medicine for the treatment of pathological conditions involving opiate receptors, in particular severe acute or chronic pains, drug addition or the prevention or the treatment of transplant rejections.
Plantes tr ansqenlque s > Controlled gene expression in plants (K Palme et ai, DE, UK) Max-Planck-Gesellschaft zur Forderung der Wissenchaften eV N°823480, EP. This invention relates to the control of gene expression in plants using either a chimeric transcriptional activator protein system, or a system based on suppressor tRNAs. (... )
Methodes d'amplification > Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific Tm (M Becker and M Majlessi, US) Gen-Probe Inc N° we 98/02582, PCT. The present invention concerns oligonucleotides containing one or more modified nucleotides which increased the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. (... )
Divers > Protein involved in re-
generating luciferin (N Kajiyama, JP)
Kikkoman Corp N°825257, EP. A protein having the ability to regenerate luciferin by acting on oxyluciferin and Dcysteine. By adding this protein to a luciferin/luciferase reaction system, the luminescence can persist and the amount of luciferase and luciferin used can be reduced.
>
Pressure~mediated binding of bion»leculer complexe. (OJ Litt at ai, US) Bioseq Inc and the inventors N" we 98/00032. PCT The invention relates to pressure-mediated dissociation of an analyte complexed with an endogenous binding partner to enable detection of a complex formed from the analyte and an exogenous binding factor, pressure-mediated association of an analyte and an exogenous binding partner to enable more rapid andlor more sensitive detection of an analyre, and pressure-mediated association and dissociation of biomolecular complexes to enable separation of one biomolecule from a complexe mixture. (... )
> High-throughput scree-
ning asay systems in mlcroscale fluidic devices (JW Paree et ai, US) Caliper Technologies Corp and the inventors N" WO 98/00231, PCT. The present invention provides microfluidic devices and methods that are useful for performing highthroughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems. (... )
> A recombinant enzyme
with mutlnase ~ (JA Wahleithner et ai, US, DK) Novo Nordisk NS and the inventors N°we 98/00528, PCT The present invention relates to method for constructing an expression vector comprising a rnutanase gene obtained from a filamentous fungus suitable for heterologous production cornpri-
sing the steps of isolating a DNA sequence encoding a mutanase from a filamentous fungus, introducing a kex2 or kex2-like site between the DNA sequences encoding the pro-peptide and the mature region of the mutanase, cloning the DNA sequence obtained in step into a suitable expression vector. (... )
> Composition and methods for modulating the length of telomeres (B Chabot, CAl Universita de Sherbrooke and the inventor N°we 98/00537, peT.
> Denaturation of double-
stranded nucleic acid (DC Purvis and S Watson, GB) Scientific Generics Ltd and the inventors N" we 98/00562, PCT. Double-stranded nucleic acid is denatured by subjecting a solution thereof to a voltage applied between electrodes spaced by no more than 1.5 mm in a time not previously achievable in electrochemical denaturation (... ) > Enzymatically removable linkers for solid phase synthesis (H Waldmann et ai, DE) BAF AG and the inventors N°we 98/01406, PCT > Determination of anaIytes by means of two markers (U Giesen et el, DE) Boehringer Mannheim GmbH and the inventors N" WO 98/01578, PCT. The combination of two detection mechanisms in a single apparatus leads to a process that enables the detected analytes to be easily quantified. Appropriate formats and reagent kits are disclosed. > Systeme de detection des interaction protelneprotelne (M Granger et M Schnarr, FR) Cnrs et les inventeurs N" we 98/01579, PCT. La presente invention concerne I'utilisation d'une sequence d'ADN cornprenant : une sequence nucleotidique codant une proteine indicatrice (gene indicateur), un prornoteur, un operareur